scholarly journals Macrophages Regulate Smooth Muscle Differentiation of Mesenchymal Stem Cells via a Prostaglandin F 2α −Mediated Paracrine Mechanism

2012 ◽  
Vol 32 (11) ◽  
pp. 2733-2740 ◽  
Author(s):  
Mi Jeong Lee ◽  
Min Young Kim ◽  
Soon Chul Heo ◽  
Yang Woo Kwon ◽  
Young Mi Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Smooth Muscle ◽  
Muscle Differentiation ◽  
Prostaglandin F ◽  
Smooth Muscle Differentiation

scholarly journals Nrf3-Pla2g7 Interaction Plays an Essential Role in Smooth Muscle Differentiation From Stem Cells

2012 ◽  
Vol 32 (3) ◽  
pp. 730-744 ◽  
Author(s):  
Qingzhong Xiao ◽  
Anna E. Pepe ◽  
Gang Wang ◽  
Zhenling Luo ◽  
Li Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Essential Role ◽  
Muscle Differentiation ◽  
Smooth Muscle Differentiation

scholarly journals Nuclear Fusion-Independent Smooth Muscle Differentiation of Human Adipose-Derived Stem Cells Induced by a Smooth Muscle Environment

Stem Cells ◽  
2012 ◽  
Vol 30 (3) ◽  
pp. 481-490 ◽  
Author(s):  
Rong Zhang ◽  
Gregory S. Jack ◽  
Nagesh Rao ◽  
Patricia Zuk ◽  
Louis J. Ignarro ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Nuclear Fusion ◽  
Muscle Differentiation ◽  
Adipose Derived Stem Cells ◽  
Smooth Muscle Differentiation

scholarly journals Single-Cell RNA-Sequencing and Metabolomics Analyses Reveal the Contribution of Perivascular Adipose Tissue Stem Cells to Vascular Remodeling

2019 ◽  
Vol 39 (10) ◽  
pp. 2049-2066 ◽  
Author(s):  
Wenduo Gu ◽  
Witold N. Nowak ◽  
Yao Xie ◽  
Alexandra Le Bras ◽  
Yanhua Hu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Single Cell ◽  
Rna Sequencing ◽  
Vascular Remodeling ◽  
Muscle Differentiation ◽  
Metabolic Reprogramming ◽  
Vascular Regeneration ◽  
Single Cell Rna Sequencing ◽  
Smooth Muscle Differentiation

Objective: Perivascular adipose tissue (PVAT) plays a vital role in maintaining vascular homeostasis. However, most studies ascribed the function of PVAT in vascular remodeling to adipokines secreted by the perivascular adipocytes. Whether mesenchymal stem cells exist in PVAT and play a role in vascular regeneration remain unknown. Approach and Results: Single-cell RNA-sequencing allowed direct visualization of the heterogeneous PVAT-derived mesenchymal stem cells (PV-ADSCs) at a high resolution and revealed 2 distinct subpopulations, among which one featured signaling pathways crucial for smooth muscle differentiation. Pseudotime analysis of cultured PV-ADSCs unraveled their smooth muscle differentiation trajectory. Transplantation of cultured PV-ADSCs in mouse vein graft model suggested the contribution of PV-ADSCs to vascular remodeling through smooth muscle differentiation. Mechanistically, treatment with TGF-β1 (transforming growth factor β1) and transfection of microRNA (miR)-378a-3p mimics induced a similar metabolic reprogramming of PV-ADSCs, including upregulated mitochondrial potential and altered lipid levels, such as increased cholesterol and promoted smooth muscle differentiation. Conclusions: Single-cell RNA-sequencing allows direct visualization of PV-ADSC heterogeneity at a single-cell level and uncovers 2 subpopulations with distinct signature genes and signaling pathways. The function of PVAT in vascular regeneration is partly attributed to PV-ADSCs and their differentiation towards smooth muscle lineage. Mechanistic study presents miR-378a-3p which is a potent regulator of metabolic reprogramming as a potential therapeutic target for vascular regeneration.

624 Isolation of muscle derived stem cells from rat and its smooth muscle differentiation

2004 ◽  
Vol 3 (2) ◽  
pp. 158
Author(s):  
J.Y. Lee ◽  
K. In gu ◽  
S.Y. Paik ◽  
S.H. Yuk ◽  
J.H. Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Muscle Differentiation ◽  
Smooth Muscle Differentiation
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