Trends in Apolipoprotein B, Non–High-Density Lipoprotein, and Low-Density Lipoprotein for Adults 60 Years and Older by Use of Lipid-Lowering Medications

The phosphotungstate-MgCl2 method for precipitating very low density and low density lipoproteins from plasma precipitates up to about 7 % of high density lipoprotein. The heparin-MnCl2 method, used at a final MnCl2 concentration of 0092 mol/l, does not seem to precipitate any high density lipoprotein but precipitates essentially all apolipoprotein B containing very low density and low density lipoprotein. When the final concentration of MnCl2 is 0046 mol/l, precipitation of apolipoprotein B containing very low density and low density lipoprotein is frequently incomplete. The herapin-MnCl2 method, when the MnCl2 final concentration is 0·092 mol/l, is the preferred method for high density lipoprotein cholesterol measurement if the cholesterol assay is unaffected by either heparin or MnCl2. High density lipoprotein cholesterol results, using this method, are higher than with the phosphotungstate MgCl2 method and lower than with the heparin MnCl2 method when the MnCl2 final concentration is 0·046 mol/l.

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Age and Cardiovascular Risk Attributable to Apolipoprotein B, Low‐Density Lipoprotein Cholesterol or Non‐High‐Density Lipoprotein Cholesterol

Journal of the American Heart Association ◽

10.1161/jaha.116.003665 ◽

2016 ◽

Vol 5 (10) ◽

Cited By ~ 21

Author(s):

Allan D. Sniderman ◽

Shofiqul Islam ◽

Matthew McQueen ◽

Michael Pencina ◽

Curt D. Furberg ◽

...

Keyword(s):

Cardiovascular Risk ◽

High Density Lipoprotein ◽

High Density Lipoprotein Cholesterol ◽

Apolipoprotein B ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

High Density ◽

Lipoprotein Cholesterol ◽

Low Density ◽

Low Density Lipoprotein Cholesterol

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Decreased Efficiency of Very-Low-Density Lipoprotein Lipolysis Is Linked to Both Hypertriglyceridemia and Hypercholesterolemia, but It Can Be Counteracted by High-Density Lipoprotein

Nutrients ◽

10.3390/nu13041224 ◽

2021 ◽

Vol 13 (4) ◽

pp. 1224

Author(s):

Ewa Wieczorek ◽

Agnieszka Ćwiklińska ◽

Agnieszka Kuchta ◽

Barbara Kortas-Stempak ◽

Anna Gliwińska ◽

...

Keyword(s):

High Density Lipoprotein ◽

Electrophoretic Mobility ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

High Density ◽

Lipid Lowering ◽

Lower Percentage ◽

Very Low Density Lipoprotein ◽

Low Density ◽

Vldl Triglyceride

Impaired triglyceride-rich lipoprotein plasma catabolism is considered the most important factor for hypertriglyceridemia development. The aim of this study was to evaluate the impact of hypercholesterolemia and hypertriglyceridemia on the efficiency of lipoprotein lipase (LPL)-mediated very-low-density lipoprotein (VLDL)-triglyceride lipolysis and the role of high-density lipoprotein (HDL) in this process. Subjects with no history of cardiovascular disease (CVD) and untreated with lipid-lowering agents were recruited into the study and divided into normolipidemic, hypercholesterolemic, and hyperlipidemic groups. VLDL was isolated from serum and incubated with LPL in the absence or presence of HDL. For the hypercholesterolemic and hyperlipidemic groups, a significantly lower percentage of hydrolyzed VLDL-triglyceride was achieved compared to the normolipidemic group (p < 0.01). HDL enhanced the lipolysis efficiency in the hypercholesterolemic and hyperlipidemic groups on average by ~7% (p < 0.001). The lowest electrophoretic mobility of the VLDL remnants indicating the most effective lipolysis was obtained in the normolipidemic group (p < 0.05). HDL presence significantly reduced the electrophoretic mobility of the VLDL remnants for the hypercholesterolemic and hyperlipidemic groups (p < 0.05). The results of our study indicate that VLDL obtained from hypercholesterolemic and hyperlipidemic subjects are more resistant to lipolysis and are additional evidence of the need for early implementation of hypocholesterolemic treatment, already in asymptomatic CVD subjects.

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