scholarly journals Detection of monoclonal protein by capillary zone electrophoresis can be challenged by iodinated contrast agent interference: a case report

2021 ◽  
Vol 31 (2) ◽  
Author(s):  
Clément Capaldo ◽  
Mourad Cheddad El Aouni ◽  
Damien Laurelli ◽  
Cyril Leven ◽  
Jean-Luc Carré
Keyword(s):  
Case Report ◽  
Contrast Agent ◽  
Capillary Zone Electrophoresis ◽  
Monoclonal Protein ◽  
Iodinated Contrast ◽  
Zone Electrophoresis ◽  
Iodinated Contrast Agent ◽  
Capillary Zone

False-Negative Result in the Detection of an IgM Monoclonal Protein by Capillary Zone Electrophoresis

2004 ◽  
Vol 50 (10) ◽  
pp. 1878-1880 ◽  
Author(s):  
Henrik Zetterberg ◽  
Herman Nilsson-Ehle
Keyword(s):  
Capillary Zone Electrophoresis ◽  
False Negative ◽  
False Negative Result ◽  
Monoclonal Protein ◽  
Zone Electrophoresis ◽  
Capillary Zone

Large abnormal peak on capillary zone electrophoresis due to contrast agent

2017 ◽  
Vol 55 (5) ◽  
pp. 608-611
Author(s):  
Rachel D Wheeler ◽  
Liqun Zhang ◽  
Joanna Sheldon
Keyword(s):  
Contrast Agent ◽  
Serum Sample ◽  
Capillary Zone Electrophoresis ◽  
Total Protein ◽  
Gel Electrophoresis ◽  
Normal Serum ◽  
Ct Angiogram ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Large Peak

Background Some iodinated radio-contrast media absorb ultraviolet light and can therefore be detected by capillary zone electrophoresis. If seen, these peaks are typically small with ‘quantifications’ of below 5 g/L. Here, we describe the detection of a large peak on capillary zone electrophoresis that was due to the radio-contrast agent, Omnipaque™. Methods Serum from a patient was analysed by capillary zone electrophoresis, and the IgG, IgA, IgM and total protein concentrations were measured. The serum sample was further analysed by gel electrophoresis and immunofixation. Results Capillary zone electrophoresis results for the serum sample showed a large peak with a concentration high enough to warrant urgent investigation. However, careful interpretation alongside the serum immunoglobulin concentrations and total protein concentration showed that the abnormal peak was a pseudoparaprotein rather than a monoclonal immunoglobulin. This was confirmed by analysis with gel electrophoresis and also serum immunofixation. The patient had had a CT angiogram with the radio-contrast agent Omnipaque™; addition of Omnipaque™ to a normal serum sample gave a peak with comparable mobility to the pseudoparaprotein in the patient’s serum. Conclusions Pseudoparaproteins can appear as a large band on capillary zone electrophoresis. This case highlights the importance of a laboratory process that detects significant electrophoretic abnormalities promptly and interprets them in the context of the immunoglobulin concentrations. This should avoid incorrect reporting of pseudoparaproteins which could result in the patient having unnecessary investigations.

scholarly journals Interference of Iodinated Contrast Media in Serum Capillary Zone Electrophoresis

2000 ◽  
Vol 46 (5) ◽  
pp. 736-737 ◽  
Author(s):  
María Luisa Arranz-Peña ◽  
Manuel González-Sagrado ◽  
Ana María Olmos-Linares ◽  
Nuria Fernández-García ◽  
Francisco Javier Martín-Gil
Keyword(s):  
Contrast Media ◽  
Capillary Zone Electrophoresis ◽  
Iodinated Contrast Media ◽  
Iodinated Contrast ◽  
Zone Electrophoresis ◽  
Capillary Zone

Physiology of Intraluminal Administration of Carbon Dioxide as a Contrast Medium

2020 ◽  
Vol 25 (44) ◽  
pp. 4656-4661 ◽  
Author(s):  
Nikolaos Patelis ◽  
Mikes Doulaptsis ◽  
Stylianos Kykalos ◽  
Eleftherios Spartalis ◽  
Anastasios Maskanakis ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Contrast Agent ◽  
20Th Century ◽  
English Literature ◽  
Human Organism ◽  
Digital Subtraction ◽  
Extensive Search ◽  
Iodinated Contrast ◽  
Iodinated Contrast Agent ◽  
Carbon Dioxide Co2

Background: Carbon dioxide (CO2) exists in nature around us. In the middle of the 20th century, the intraluminal injection of CO2 demonstrated similar results to those of Digital Subtraction Angiography (DSA) with an iodinated contrast agent (ICA). Since then, the technology behind CO2 DSA has developed significantly. Objective: The aim of this study is to inform physicians about the unique properties of CO2 and its physiology after intraluminal injection. Methods: An extensive search for English literature on the properties of CO2 and the physiology of intraluminal administration was conducted using Pubmed. Results: There is sufficient literature on the properties of CO2 and the physiology of CO2 DSA. A review of this literature explains what happens to the human organism after the injection of CO2. Conclusions: There is enough evidence that CO2 DSA is both effective, diagnostic and safe, but the properties of CO2 should be taken under consideration as complications occur, although rarely.

Zoledronate and Related Impurities Analysis by Capillary Zone Electrophoresis

2014 ◽  
Vol 10 (2) ◽  
pp. 231-234 ◽  
Author(s):  
Gisela Alvarez ◽  
María Foglia ◽  
Daniela Camporotondi ◽  
S. Giorgieri ◽  
Martín Desimone ◽  
...  
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Related Impurities ◽  
Zone Electrophoresis ◽  
Capillary Zone

Investigation of structure–mobility relationships and estimation of secondary structure of biopeptides by capillary zone electrophoresis

2005 ◽  
Author(s):  
Veronika Šolínová ◽  
Václav Kašička ◽  
Dušan Koval ◽  
Jan Hlaváček ◽  
Tomislav Barth
Keyword(s):  
Secondary Structure ◽  
Capillary Zone Electrophoresis ◽  
Zone Electrophoresis ◽  
Capillary Zone
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