Devibursaphelenchus alienae n. sp. isolated from the bark of dead Quercus aliena in Japan

Nematology ◽  
2021 ◽  
pp. 1-20
Author(s):  
Natsumi Kanzaki ◽  
Keiko Hamaguchi ◽  
Yuko Takeuchi-Kaneko
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Ribosomal Rna ◽  
Large Body ◽  
Oak Wilt ◽  
Japanese Oak Wilt ◽  
Female Tail ◽  
Tail Shape ◽  
Molecular Phylogenetic ◽  
Rna Genes

Summary A new Devibursaphelenchus species isolated from the bark of a dead Quercus aliena, which had been infected and killed by Japanese oak wilt, was collected in Shiga, Japan. The new species is characterised by the relatively large body in males (661-768 μm) and females (893-1071 μm), conspicuous male bursal flap, male spicule with long condylus and wide blade, female post-vulval uterine sac (PUS) 39-54 μm or 1.6-2.3 times the vulval body diam. long, vestigial female anus, and female tail forming a strongly ventrally recurved elongate conoid with bluntly pointed or narrowly rounded terminus. The new species is typologically similar to D. lini, sharing a large body, conspicuous bursal flap, long PUS, and spicule shape, but can be distinguished from it by the absence of variation in the female tail shape, i.e., the tail of the new species is always long and strongly ventrally curved, while the tail shape varies more in D. lini. A previous molecular phylogenetic analysis suggested that the new species is fairly close to D. lini, but can be distinguished from it by the 1.0% (16 bp) difference within 1.6 kb of the 18S and 3.7% (26 bps) difference within 0.7 kb of the D2-D3 LSU ribosomal RNA genes. The newly found nematode is described and illustrated herein as D. alienae n. sp.

Sheraphelenchus sucus n. sp. (Tylenchina: Aphelenchoididae) isolated from sap flow of Quercus serrata in Japan

Nematology ◽  
2013 ◽  
Vol 15 (8) ◽  
pp. 975-990 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Ryusei Tanaka
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Ribosomal Rna ◽  
Sap Flow ◽  
Additional Data ◽  
Quercus Serrata ◽  
Phylogenetic Status ◽  
Molecular Phylogenetic ◽  
Molecular Barcode ◽  
Rna Genes

A Sheraphelenchus species was isolated from a sample of sap exuding from a scar on the bark of Quercus serrata. Besides its generic characteristics, i.e., posteriorly located vulva in the female, male spicule with conspicuous dorsal limb and male tail with spike-like projection, the new species is characterised by a short stylet with a small basal swelling. The near-full-length of 18S and D2/D3 expansion segments of ribosomal RNA genes (near-full SSU and D2/D3LSU) were determined as its molecular barcode sequences and the phylogenetic status of the species (= genus Sheraphelenchus) was estimated using the near-full SSU. The molecular phylogenetic analysis revealed that the new species is included in the genus Bursaphelenchus, and is close to B. anatolius and B. kevini. Although Sheraphelenchus is phylogenetically inferred to occur within Bursaphelenchus, the new species is described herein as S. sucus n. sp., thereby retaining the genus Sheraphelenchus until additional data about morphological similarities between these two taxa are obtained.

Description of Ektaphelenchoides spondylis n. sp. (Nematoda: Ektaphelenchinae) isolated from Spondylis buprestoides (Coleoptera: Cerambycidae) in Japan

Nematology ◽  
2009 ◽  
Vol 11 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Robin Giblin-Davis ◽  
Barbara Center
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Large Subunit ◽  
Taxonomic Revision ◽  
Molecular Phylogenetic Analysis ◽  
Molecular Sequence ◽  
Male Ratio ◽  
Female Tail ◽  
Tail Shape ◽  
Molecular Phylogenetic

AbstractEktaphelenchoides spondylis n. sp. is described and figured from Spondylis buprestoides. The new species is characterised by the bipartite stylet conus, male spicule with rounded condylus, blunt rostrum and plate-like cucullus, long postuterine sac and long and smoothly tapering female tail. The new species is almost morphologically identical to E. compsi, but can be distinguished from it by stylet morphology, female tail shape, male ratio (a) and molecular sequence of the D2/D3 expansion segment of the large subunit of ribosomal DNA. In the molecular phylogenetic analysis, E. spondylis n. sp. formed a well supported clade with E. compsi within Ektaphelenchoides. Based upon molecular phylogenetic analysis with one locus, Ektaphelenchoides spp. formed a well supported clade and Ektaphelenchus and Cryptaphelenchus also formed a well supported clade. However, the molecular and morphological information available from our study are insufficient to revise the generic taxonomy of the subfamily. More sampling and an integrated taxonomic revision of the subfamily Ektaphelenchinae are needed.

Description of Bursaphelenchus arthuroides sp. n. (Nematoda: Aphelenchoididae), a second parthenogenetic species of Bursaphelenchus Fuchs, 1937

Nematology ◽  
2012 ◽  
Vol 14 (1) ◽  
pp. 51-63 ◽  
Author(s):  
Jianfeng Gu ◽  
Jiangling Wang ◽  
Jingwu Zheng
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Molecular Phylogenetic Analysis ◽  
Dorsal Part ◽  
Species Status ◽  
Lateral Field ◽  
Middle Position ◽  
Parthenogenetic Species ◽  
Female Tail ◽  
Molecular Phylogenetic

Bursaphelenchus arthuroides sp. n. is described and figured from pine packaging wood originating in Brazil, and inspected in Ningbo harbour, P. R. China. The new species clearly belongs to the fungivorus group. It is characterised by four lines in the lateral field; spicules medium sized (15-17 μm), dorsal part distinctly sclerotised, with a high rounded condylus and a blunted pointed rostrum in the middle position, capitulum with a shallow depression, the posterior third of the dorsal limb ventrally bent, distal end broadly rounded without cucullus; the shape of the female tail conical and slim, posterior third distinctly ventrally bent with a finely pointed terminus; vulval lips slightly protruding but not forming a vulval flap and post-uterine sac extending for about half the vulva-anus distance. Species status is supported by ITS-RFLP patterns and molecular phylogenetic analysis based on partial LSU sequences, ITS1/2 and partial LSU sequences, which revealed that B. arthuroides sp. n. is closest to B. arthuri. Propagation tests also confirmed that the new species is parthenogenetic.

Description of Bursaphelenchus paraparvispicularis n. sp. (Nematoda: Aphelenchoididae) found in packaging wood from Hongkong, China

Nematology ◽  
2010 ◽  
Vol 12 (4) ◽  
pp. 557-566 ◽  
Author(s):  
Jianfeng Gu ◽  
Jiangling Wang ◽  
Weijun Duan ◽  
Helen Braasch ◽  
Wolfgang Burgermeister ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Molecular Phylogenetic Analysis ◽  
Species Status ◽  
Separate Species ◽  
Female Ratio ◽  
Lateral Field ◽  
Female Tail ◽  
Molecular Phylogenetic ◽  
Males And Females

Abstract Bursaphelenchus paraparvispicularis n. sp. is described and figured from pine packaging wood originating in Hongkong, China, and inspected in Ningbo harbour, China. The new species clearly belongs to the hofmanni group. It is characterised by a relatively stout body (a = 26.7 and 26.5 for males and females, respectively), three lines in the lateral field, seven caudal papillae, spicules relatively small (12.6-15.3 μm), mitten-shaped, with lamina dorsal line smoothly arcuate but calomus relatively straight, condylus squared or round, well developed, rostrum well developed with round terminus, cucullus absent, the shape of the female tail, which is short and ventrally bent with a bluntly pointed terminus, and vulval lips not forming a vulval flap. The new species is morphologically closest to B. parvispicularis and can be distinguished by smaller and stouter body, lower female ratio c′ (average 2.8 vs 4.4) and longer spicule condylus. The separate species status is supported by ITS-RFLP patterns and molecular phylogenetic analysis based on ITS1/2 and partial LSU sequences, which revealed that B. paraparvispicularis n. sp. is closest to B. parvispicularis.

Description and phylogeny of Bursaphelenchus luxuriosae n. sp. (Nematoda: Aphelenchoididae) isolated from Acalolepta luxuriosa (Coleoptera: Cerambycidae)

Nematology ◽  
2003 ◽  
Vol 5 (4) ◽  
pp. 565-572 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Kazuyoshi Futai
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Botrytis Cinerea ◽  
Bursaphelenchus Xylophilus ◽  
Lateral Field ◽  
Female Tail ◽  
Phylogenetic Status ◽  
Rflp Profile ◽  
Molecular Phylogenetic ◽  
Males And Females

AbstractBursaphelenchus luxuriosae n. sp. is described and figured. Specimens were collected from a 2-week-old culture on Botrytis cinerea. The new species is characterised by a body length of 897 (710-1159) μm in the female and 745 (621-887) μm in the male, relatively robust body (a = 33-39 in the female and 27-30 in the male), stylet ca 14 (11-16) μm long, four lines in the lateral field, the large (27-30 μm) arcuate spicule with a terminal cucullus, seven (2 + 1 + 2 + 2) male caudal papillae, the long, well developed vulval flap and the shape of the female tail which is long, tapered, and ventrally bent when killed by heat with an irregular or roughened dorsal contour near the tip and an irregular terminus. The new species is considered to belong to the Bursaphelenchus xylophilus group of the genus Bursaphelenchus and is most closely related to B. conicaudatus and B. fraudulentus in spicule shape, vulval flap and 'a' values of males and females. It is easily distinguished from these two species by the morphology of female tail. The RFLP profile confirms the distinctness of the new species within the B. xylophilus group. The phylogenetic status of B. luxuriosae n. sp. within the B. xylophilus group is indicated by molecular phylogenetic analysis. Bursaphelenchus luxuriosae n. sp. is assumed to be close to B. conicaudatus and to have diverged from the ancestor of the B. xylophilus group early in the speciation of the group.

Description of Ruehmaphelenchus fujiensis n. sp. (Tylenchomorpha: Aphelenchoididae) isolated from dead wood of Quercus crispula from Yamanashi, Japan

Nematology ◽  
2021 ◽  
pp. 1-18
Author(s):  
Natsumi Kanzaki
Keyword(s):  
New Species ◽  
Cryptic Species ◽  
Botrytis Cinerea ◽  
Ribosomal Rna ◽  
Dead Wood ◽  
Large Subunit ◽  
Small Subunit ◽  
Quercus Crispula ◽  
Female Tail ◽  
Molecular Phylogenetic

Summary Three undescribed Ruehmaphelenchus species were isolated from dead wood of Quercus crispula collected in three different localities in Japan. One of these three species was successfully cultured using Botrytis cinerea as its food fungus and is described and figured as R. fujiensis n. sp. The new species is characterised by the following features: male tail, conical with or without a projection; female tail, conical with variously shaped terminal mucron or projection; and male spicule possessing a relatively long and triangular condylus. The new species is close to, or almost a cryptic species of R. digitulus, i.e., the typological characters and morphometric values of these two species are mostly overlapping. In a molecular phylogenetic relationship inferred from near-full-length small subunit (18S) and D2-D3 expansion segments of the large subunit (28S) of ribosomal RNA, the new species is close to R. asiaticus, R. digitulus, R. formosanus, and Ruehmaphelenchus sp. NKZ202; however, it was clearly separated from these species.

Description of Basilaphelenchus brevicaudatus n. sp. (Aphelenchoidea: Tylaphelenchinae) from a dead forest tree in northern Iran

Nematology ◽  
2019 ◽  
Vol 21 (2) ◽  
pp. 147-158 ◽  
Author(s):  
Zeinab Mirzaie Fouladvand ◽  
Ebrahim Pourjam ◽  
Natsumi Kanzaki ◽  
Robin M. Giblin-Davis ◽  
Majid Pedram
Keyword(s):  
Body Length ◽  
Ribosomal Rna ◽  
Large Subunit ◽  
Forest Tree ◽  
Northern Iran ◽  
Female Tail ◽  
Molecular Phylogenetic ◽  
Golestan Province ◽  
Tail Tip ◽  
Rna Genes

Summary Basilaphelenchus brevicaudatus n. sp., the third species of this apparently rare genus, is described and illustrated. It was recovered from wood and bark samples from a dead forest tree in the Golestan province of northern Iran. It is typologically characterised by female body length (448 (365-492) μm), three lines in the lateral fields, raised cephalic region having a sclerotised vestibule and cephalic framework, stylet thin with delicate conus and thicker shaft, both parts lacking a visible lumen, and with three elongate, backwardly directed knobs, small, spherical to spade-shaped metacorpus with small, posteriorly located valve (at 72 (58-74)% of metacorpus length), simple vulva without flap at 72.5 (69-78)% of body length, post-vulval uterine sac 32.4 (29.0-37.0) μm long, functional rectum and anus, female tail conical, short (c′ = 2.6 (1.9-3.3) in female, and 2.5 (2.3-2.8) in male), dorsally convex and ventrally concave with blunt end or having a small mucron, both forms with a hyaline-like tip. Males common, with well-curved 9.2 (9.0-10.5) μm long spicules measured along the mid-line, three pairs of small caudal papillae (lacking the single P1 ventral papilla) and no bursa at tail tip, but with hyaline region, similar to females. Basilaphelenchus brevicaudatus n. sp. is compared with the two currently known species of the genus, the type species, B. persicus, and B. grosmannae. Molecular phylogenetic inferences using partial sequences of small and large subunit ribosomal RNA genes (SSU and LSU) from different isolates of the new species revealed that it belongs to the Tylaphelenchinae clade.

Description of Ruehmaphelenchus digitulus sp. n. (Nematoda: Aphelenchoididae) found in packaging wood from Taiwan

Nematology ◽  
2012 ◽  
Vol 14 (4) ◽  
pp. 489-498 ◽  
Author(s):  
Jianfeng Gu ◽  
Jiangling Wang
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Rflp Pattern ◽  
Species Status ◽  
Separate Species ◽  
Lateral Field ◽  
Male And Female ◽  
Female Tail ◽  
Molecular Phylogenetic ◽  
Shape And Size

Ruehmaphelenchus digitulus sp. n., isolated from coniferous packaging wood from Taiwan, is described and figured. The new species is characterised by a relatively stout body with a = 28.0 (23.7-31.3) and 27.0 (22.9-32.7) for male and female, respectively, three lines in the lateral field, spicules relatively small (chord = 17.0-19.2 μm; arc = 13.8-16.6 μm) with weakly developed condylus and rostrum, bursa absent, vulva positioned at ca 80% of body length, vulva flap absent, vulval lips slightly protruding, post-uterine branch extending slightly over half of vulva to anus distance, and tail conoid, ca 2-3 anal body diam. long, with terminal process 7-10 μm long. The new species is the fourth known for the genus. It can be distinguished from R. martinii, R. asiaticus and R. thailandae mainly by the different shape and size of the spicules and the different shape and length of the female tail terminus. The separate species status is also supported by ITS-RFLP pattern and molecular phylogenetic analysis based on partial SSU ITS1/2 and partial LSU sequences.

Molecular phylogeny of freshwater snails and limpets (Panpulmonata: Hygrophila)

2020 ◽  
Vol 190 (2) ◽  
pp. 518-531 ◽  
Author(s):  
Ahmed J Saadi ◽  
Angus Davison ◽  
Christopher M Wade
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Phylogeny ◽  
Ribosomal Rna ◽  
Large Subunit ◽  
Taxonomic Revision ◽  
Monophyletic Group ◽  
Molecular Phylogenetic Analysis ◽  
Freshwater Snails ◽  
Molecular Phylogenetic ◽  
Rna Genes

Abstract We have undertaken a comprehensive molecular phylogenetic analysis of the Hygrophila based on 3112 sites of the large subunit and 5.8S ribosomal RNA genes. A clear basal division between Chilinoidea and Lymnaeoidea is observed. The monophyly of Acroloxidae, Lymnaeidae and Physidae is also well established. However, Planorbidae are not supported as a monophyletic group, because the Bulinidae cluster within Planorbidae. The Amphipepleinae within Lymnaeidae and both Planorbinae and Ancylinae within Planorbidae are strongly supported as monophyletic subfamilies. However, the Aplexinae within Physidae and the Lymnaeinae within Lymnaeidae are not recovered. A new taxonomic revision of the Hygrophila is proposed based on the findings of this molecular phylogeny, and the implications for the evolution of chirality are discussed.

Description of Bursaphelenchus yuyaoensis n. sp. (Nematoda: Aphelenchoididae) isolated from Pinus massoniana in China

Nematology ◽  
2014 ◽  
Vol 16 (4) ◽  
pp. 411-418 ◽  
Author(s):  
Jianfeng Gu ◽  
Jie He ◽  
Jiangling Wang ◽  
Xianfeng Chen
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Pinus Massoniana ◽  
Species Status ◽  
Separate Species ◽  
Lateral Field ◽  
Male Tail ◽  
Female Tail ◽  
Molecular Phylogenetic ◽  
Males And Females

Bursaphelenchus yuyaoensisn. sp. is described and figured from dyingPinus massonianain Yuyao, China. The new species clearly belongs to thehofmannigroup. It is characterised by relatively slim body (a = 43.7 and 36.2 for males and females, respectively), three lines in the lateral field, spicules relatively small (11.4-14.7 μm), mitten-shaped with the posterior third of the dorsal limb distinctly curved, condylus round and slightly dorsally bent, rostrum well developed with more or less rounded terminus, distal ends of spicules not forming a clear cucullus, male tail with a mucron-like process, female tail slightly ventrally bent with a bluntly rounded terminus, vulval lip not forming a vulval flap and post-uterine sac extending for about half the vulva-anus distance. The separate species status is supported by ITS-RFLP patterns and molecular phylogenetic analysis based on ITS1/2 sequences, which revealed thatB. yuyaoensisn. sp. is close toB. parvispicularisandB. paraparvispicularis.

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