Description of Ruehmaphelenchus digitulus sp. n. (Nematoda: Aphelenchoididae) found in packaging wood from Taiwan

Nematology ◽  
2012 ◽  
Vol 14 (4) ◽  
pp. 489-498 ◽  
Author(s):  
Jianfeng Gu ◽  
Jiangling Wang
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Rflp Pattern ◽  
Species Status ◽  
Separate Species ◽  
Lateral Field ◽  
Male And Female ◽  
Female Tail ◽  
Molecular Phylogenetic ◽  
Shape And Size

Ruehmaphelenchus digitulus sp. n., isolated from coniferous packaging wood from Taiwan, is described and figured. The new species is characterised by a relatively stout body with a = 28.0 (23.7-31.3) and 27.0 (22.9-32.7) for male and female, respectively, three lines in the lateral field, spicules relatively small (chord = 17.0-19.2 μm; arc = 13.8-16.6 μm) with weakly developed condylus and rostrum, bursa absent, vulva positioned at ca 80% of body length, vulva flap absent, vulval lips slightly protruding, post-uterine branch extending slightly over half of vulva to anus distance, and tail conoid, ca 2-3 anal body diam. long, with terminal process 7-10 μm long. The new species is the fourth known for the genus. It can be distinguished from R. martinii, R. asiaticus and R. thailandae mainly by the different shape and size of the spicules and the different shape and length of the female tail terminus. The separate species status is also supported by ITS-RFLP pattern and molecular phylogenetic analysis based on partial SSU ITS1/2 and partial LSU sequences.

Description of Bursaphelenchus paraparvispicularis n. sp. (Nematoda: Aphelenchoididae) found in packaging wood from Hongkong, China

Nematology ◽  
2010 ◽  
Vol 12 (4) ◽  
pp. 557-566 ◽  
Author(s):  
Jianfeng Gu ◽  
Jiangling Wang ◽  
Weijun Duan ◽  
Helen Braasch ◽  
Wolfgang Burgermeister ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Molecular Phylogenetic Analysis ◽  
Species Status ◽  
Separate Species ◽  
Female Ratio ◽  
Lateral Field ◽  
Female Tail ◽  
Molecular Phylogenetic ◽  
Males And Females

Abstract Bursaphelenchus paraparvispicularis n. sp. is described and figured from pine packaging wood originating in Hongkong, China, and inspected in Ningbo harbour, China. The new species clearly belongs to the hofmanni group. It is characterised by a relatively stout body (a = 26.7 and 26.5 for males and females, respectively), three lines in the lateral field, seven caudal papillae, spicules relatively small (12.6-15.3 μm), mitten-shaped, with lamina dorsal line smoothly arcuate but calomus relatively straight, condylus squared or round, well developed, rostrum well developed with round terminus, cucullus absent, the shape of the female tail, which is short and ventrally bent with a bluntly pointed terminus, and vulval lips not forming a vulval flap. The new species is morphologically closest to B. parvispicularis and can be distinguished by smaller and stouter body, lower female ratio c′ (average 2.8 vs 4.4) and longer spicule condylus. The separate species status is supported by ITS-RFLP patterns and molecular phylogenetic analysis based on ITS1/2 and partial LSU sequences, which revealed that B. paraparvispicularis n. sp. is closest to B. parvispicularis.

Description of Ruehmaphelenchus thailandae n. sp. (Nematoda: Aphelenchoididae) found in dunnage from Thailand

Nematology ◽  
2010 ◽  
Vol 12 (6) ◽  
pp. 869-876 ◽  
Author(s):  
Jianfeng Gu ◽  
Jiangling Wang
Keyword(s):  
New Species ◽  
Body Length ◽  
Rflp Pattern ◽  
Species Status ◽  
Separate Species ◽  
Lateral Field ◽  
The Third ◽  
Female Tail ◽  
Males And Females ◽  
Shape And Size

AbstractRuehmaphelenchus thailandae n. sp., isolated from deciduous dunnage from Thailand, is described and figured. The new species is characterised by the relatively stout body (a = 28.6 and 27.6 for males and females, respectively), three lines in the lateral field, spicules relatively small (12.0-13.3 μm) with weakly developed condylus and rostrum, bursa absent, vulva located at 79.5% of body length, vulval lips slightly swollen, length of post-uterine branch more than half of vulva to anus distance, female tail conoid, ca two anal body diam. long and with a broad terminal process up to 12 μm long. The new species is the third known for the genus. It can be distinguished from R. martinii and R. asiaticus mainly by the different shape and size of its spicules and different shape of the female tail terminus. The separate species status is also supported by ITS-RFLP pattern. Additional measurements of paratype R. asiaticus are appended.

Description of Bursaphelenchus yuyaoensis n. sp. (Nematoda: Aphelenchoididae) isolated from Pinus massoniana in China

Nematology ◽  
2014 ◽  
Vol 16 (4) ◽  
pp. 411-418 ◽  
Author(s):  
Jianfeng Gu ◽  
Jie He ◽  
Jiangling Wang ◽  
Xianfeng Chen
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Pinus Massoniana ◽  
Species Status ◽  
Separate Species ◽  
Lateral Field ◽  
Male Tail ◽  
Female Tail ◽  
Molecular Phylogenetic ◽  
Males And Females

Bursaphelenchus yuyaoensisn. sp. is described and figured from dyingPinus massonianain Yuyao, China. The new species clearly belongs to thehofmannigroup. It is characterised by relatively slim body (a = 43.7 and 36.2 for males and females, respectively), three lines in the lateral field, spicules relatively small (11.4-14.7 μm), mitten-shaped with the posterior third of the dorsal limb distinctly curved, condylus round and slightly dorsally bent, rostrum well developed with more or less rounded terminus, distal ends of spicules not forming a clear cucullus, male tail with a mucron-like process, female tail slightly ventrally bent with a bluntly rounded terminus, vulval lip not forming a vulval flap and post-uterine sac extending for about half the vulva-anus distance. The separate species status is supported by ITS-RFLP patterns and molecular phylogenetic analysis based on ITS1/2 sequences, which revealed thatB. yuyaoensisn. sp. is close toB. parvispicularisandB. paraparvispicularis.

Description of Bursaphelenchus arthuroides sp. n. (Nematoda: Aphelenchoididae), a second parthenogenetic species of Bursaphelenchus Fuchs, 1937

Nematology ◽  
2012 ◽  
Vol 14 (1) ◽  
pp. 51-63 ◽  
Author(s):  
Jianfeng Gu ◽  
Jiangling Wang ◽  
Jingwu Zheng
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Molecular Phylogenetic Analysis ◽  
Dorsal Part ◽  
Species Status ◽  
Lateral Field ◽  
Middle Position ◽  
Parthenogenetic Species ◽  
Female Tail ◽  
Molecular Phylogenetic

Bursaphelenchus arthuroides sp. n. is described and figured from pine packaging wood originating in Brazil, and inspected in Ningbo harbour, P. R. China. The new species clearly belongs to the fungivorus group. It is characterised by four lines in the lateral field; spicules medium sized (15-17 μm), dorsal part distinctly sclerotised, with a high rounded condylus and a blunted pointed rostrum in the middle position, capitulum with a shallow depression, the posterior third of the dorsal limb ventrally bent, distal end broadly rounded without cucullus; the shape of the female tail conical and slim, posterior third distinctly ventrally bent with a finely pointed terminus; vulval lips slightly protruding but not forming a vulval flap and post-uterine sac extending for about half the vulva-anus distance. Species status is supported by ITS-RFLP patterns and molecular phylogenetic analysis based on partial LSU sequences, ITS1/2 and partial LSU sequences, which revealed that B. arthuroides sp. n. is closest to B. arthuri. Propagation tests also confirmed that the new species is parthenogenetic.

Description and phylogeny of Bursaphelenchus luxuriosae n. sp. (Nematoda: Aphelenchoididae) isolated from Acalolepta luxuriosa (Coleoptera: Cerambycidae)

Nematology ◽  
2003 ◽  
Vol 5 (4) ◽  
pp. 565-572 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Kazuyoshi Futai
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Botrytis Cinerea ◽  
Bursaphelenchus Xylophilus ◽  
Lateral Field ◽  
Female Tail ◽  
Phylogenetic Status ◽  
Rflp Profile ◽  
Molecular Phylogenetic ◽  
Males And Females

AbstractBursaphelenchus luxuriosae n. sp. is described and figured. Specimens were collected from a 2-week-old culture on Botrytis cinerea. The new species is characterised by a body length of 897 (710-1159) μm in the female and 745 (621-887) μm in the male, relatively robust body (a = 33-39 in the female and 27-30 in the male), stylet ca 14 (11-16) μm long, four lines in the lateral field, the large (27-30 μm) arcuate spicule with a terminal cucullus, seven (2 + 1 + 2 + 2) male caudal papillae, the long, well developed vulval flap and the shape of the female tail which is long, tapered, and ventrally bent when killed by heat with an irregular or roughened dorsal contour near the tip and an irregular terminus. The new species is considered to belong to the Bursaphelenchus xylophilus group of the genus Bursaphelenchus and is most closely related to B. conicaudatus and B. fraudulentus in spicule shape, vulval flap and 'a' values of males and females. It is easily distinguished from these two species by the morphology of female tail. The RFLP profile confirms the distinctness of the new species within the B. xylophilus group. The phylogenetic status of B. luxuriosae n. sp. within the B. xylophilus group is indicated by molecular phylogenetic analysis. Bursaphelenchus luxuriosae n. sp. is assumed to be close to B. conicaudatus and to have diverged from the ancestor of the B. xylophilus group early in the speciation of the group.

Description of Ektaphelenchus ibericus n. sp. (Nematoda: Ektaphelenchinae) found in packaging wood from Spain

Nematology ◽  
2013 ◽  
Vol 15 (7) ◽  
pp. 871-878 ◽  
Author(s):  
Jianfeng Gu ◽  
Jiangling Wang ◽  
Xianfeng Chen ◽  
Xuan Wang
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Molecular Phylogenetic Analysis ◽  
Species Status ◽  
Separate Species ◽  
Lateral Lines ◽  
Molecular Phylogenetic

Ektaphelenchus ibericus n. sp. is described and figured. The new species was isolated from pine packaging wood from Spain that was inspected in Ningbo harbour, China, in 2011. Ektaphelenchus ibericus n. sp. is characterised by lip region without clear constriction, relatively short stylet (11-15 μm) lacking basal knobs, and four lateral lines. The female post-uterine sac is less than one body diam. long, rectum and anus are absent, and the tail is conoid with a sharply pointed terminus. Males are rare and have a conoid tail with a sharply pointed terminus, spicules mitten-shaped, 12.8 μm long (chord) and smoothly curved with a high and bluntly rounded condylus and bluntly pointed rostrum, distal end broadly rounded, and three pairs of ventral caudal papillae present. The separate species status is also supported by molecular phylogenetic analysis based on partial SSU, ITS1/2 and partial LSU sequences.

Bursaphelenchus chengi sp. n. (Nematoda: Parasitaphelenchidae) isolated at Nanjing, China, in packaging wood from Taiwan

Nematology ◽  
2008 ◽  
Vol 10 (3) ◽  
pp. 335-346 ◽  
Author(s):  
Hongmei Li ◽  
Phap Quang Trinh ◽  
Lieven Waeyenberge ◽  
Maurice Moens
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Body Size ◽  
Posterior Margin ◽  
Petri Dish ◽  
Packaging Materials ◽  
Lateral Field ◽  
Female Tail ◽  
Domain Sequence ◽  
Lateral Lines

Abstract Bursaphelenchus chengi sp. n. is described and illustrated. Dauer juveniles were isolated from imported wood packaging materials from Taiwan to Nanjing Port, China. Bursaphelenchus chengi sp. n. was reared and maintained on Petri dish cultures of the fungus Botrytis cinerea. The new species is characterised by the medium body size in both sexes, the presence of only two incisures in the lateral field and the robust and strongly curved spicules. The spicule lamina is angular distally, the rostrum digitate and the condylus rounded. The tail is arcuate with a pointed terminus. The bursa is usually truncate with the posterior margin indented in some specimens or rounded with a fine axial point. Females have a small vulval flap formed by a short extension of the cuticle of the anterior lip, and a conical tail that gradually tapers to an almost straight or slightly recurved, pointed or rounded terminus. Because of the presence of two lateral lines, similar spicule shape, tapering female tail and the presence of a small vulval flap, B. chengi sp. n. should be grouped in the abietinus-group sensu Braasch. together with B. abietinus, B. antoniae, B. hellenicus, B. hylobianum and B. rainulfi. ITS-RFLP profiles support the proposal of the new species, and phylogenetic analysis of the 28S rDNA D2/D3 domain sequence places it close to B. antoniae and other species of the abietinus-group.

Bursaphelenchus rufipennis n. sp. (Nematoda: Parasitaphelenchinae) and redescription of Ektaphelenchus obtusus (Nematoda: Ektaphelenchinae), associates from nematangia on the hind wings of Dendroctonus rufipennis (Coleoptera: Scolytidae)

Nematology ◽  
2008 ◽  
Vol 10 (6) ◽  
pp. 925-955 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Robin M. Giblin-Davis ◽  
Yasmin J. Cardoza ◽  
Weimin Ye ◽  
Kenneth F. Raffa ◽  
...  
Keyword(s):  
New Species ◽  
Morphological Characters ◽  
Morphological Examination ◽  
Dendroctonus Rufipennis ◽  
Lateral Field ◽  
The Past ◽  
Female Tail ◽  
Molecular Phylogenetic ◽  
Voucher Specimens ◽  
Tail Tip

Abstract Two species of aphelench, Bursaphelenchus rufipennis n. sp. and Ektaphelenchus obtusus, were isolated from the 'nematangia', cocoon-like structures found at the base of the hind wings of Dendroctonus rufipennis. The nematangia contained adult females of E. obtusus and the dauer juveniles of B. rufipennis n. sp. Only B. rufipennis n. sp. could be cultured on Monilinia fructicola on LGPDA (lactic acid-treated, glycerol-supplemented, potato dextrose agar). The new species of Bursaphelenchus is described and figured and some additional morphological characters are ascribed to E. obtusus, E. josephi, E. sandiaensis, E. smaelus (= E. prolobos) and E. terebranus after examination of type and/or voucher specimens. Bursaphelenchus rufipennis n. sp. has an adult body length of ca 500-1000 μm, medium a ratios (ca 25-38 for females and ca 30-40 for males), b ratios of ca 8-13 (female) and 7-11 (male), c ratios of ca 15-22 (female and male), c′ ratios of ca 3-4 (female) and ca 2-3 (male), and is characterised by three incisures in the lateral field, mitten-shaped spicules and a conical female tail that curves ventrally and possesses a variable tail tip. The new species is morphologically closest to B. corneolus, B. curvicaudatus, B. gerberae, B. paracorneolus and B. talonus. Morphological examination of type and/or voucher specimens of five Ektaphelenchus species revealed coarse transverse body annulation and three pairs of male caudal papillae (except for the two species where males are not described). Clear typological differences were observed among these five Ektaphelenchus species in the structure of the lip region, presence/absence of stylet knobs and male spicule morphology. Although these characters have not been consistently documented in the past, they may be diagnostic for species in the genus. Molecular phylogenetic analysis based on SSU and D2/D3 LSU sequences revealed that B. rufipennis n. sp. was closest to B. paracorneolus and that E. obtusus was closest to species of Ektaphelenchoides and a Cryptaphelenchus sp.

A redefinition of the genus Troglocharinus Reitter, 1908, with description of new species (Coleoptera, Leiodidae, Cholevinae, Leptodirini)

Zootaxa ◽  
2021 ◽  
Vol 4926 (2) ◽  
pp. 151-188
Author(s):  
JAVIER FRESNEDA ◽  
VALERIA RIZZO ◽  
JORDI COMAS ◽  
IGNACIO RIBERA
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Valid Species ◽  
Distribution Area ◽  
Undescribed Species ◽  
Separate Species ◽  
Male And Female ◽  
New Material

We redefine the genus Troglocharinus Reitter, 1908 based on a phylogenetic analysis with a combination of mitochondrial and molecular data. We recovered the current Speonomites mengeli (Jeannel, 1910) and S. mercedesi (Zariquiey, 1922) as valid, separate species within the Troglocharinus clade, not directly related to Speonomites Jeannel, 1910, a finding corroborated by a detailed study of the male and female genitalia. In consequence, we reinstate Speonomus mercedesi Zariquiey, 1922 stat. nov. as a valid species, transfer both of them to the genus Troglocharinus, T. mengeli (Jeannel, 1910) comb. nov. and T. mercedesi (Zariquiey, 1922) comb. nov., and redescribe the genus. The study of new material from the distribution area of the former S. mengeli revealed the presence of two undescribed species, T. sendrai sp. nov. and T. fadriquei sp. nov., which we describe herein. We designate the lectotype of Speonomus vinyasi Escolà, 1971 to fix its identity, as among its syntypes there are two different species. In agreement with the results of the phylogenetic analyses we establish the synonymy between the genus Speonomites and Pallaresiella Fresneda, 1998 syn. nv. 

Peponium elgonense (Cucurbitaceae), a new species from Mount Elgon in Kenya

Phytotaxa ◽  
2020 ◽  
Vol 443 (2) ◽  
pp. 189-197
Author(s):  
FREDRICK MUNYAO MUTIE ◽  
PENINAH CHEPTOO RONO ◽  
VIVIAN KATHAMBI ◽  
NENG WEI ◽  
PERIS KAMAU ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Molecular Phylogenetic Analysis ◽  
Trnl Intron ◽  
Separate Species ◽  
Molecular Phylogenetic ◽  
Plastid Markers ◽  
Mount Elgon ◽  
A New Species

Peponium elgonense, a new species of Cucurbitaceae from Mount Elgon in Kenya, is described and illustrated here with photographs. This species is similar to P. cienkowskii and P. vogelii, but differs by being monoecious and having a longer ovary. A molecular phylogenetic analysis of Peponium based on ITS and five plastid markers (trnL–F, rpl20–rps12, matK, rbcL, and trnL intron) of four species of Peponium further shows that P. elgonense individuals cluster together supporting the argument to regard it as a separate species.

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