Description of Bursaphelenchus paraparvispicularis n. sp. (Nematoda: Aphelenchoididae) found in packaging wood from Hongkong, China

Nematology ◽  
2010 ◽  
Vol 12 (4) ◽  
pp. 557-566 ◽  
Author(s):  
Jianfeng Gu ◽  
Jiangling Wang ◽  
Weijun Duan ◽  
Helen Braasch ◽  
Wolfgang Burgermeister ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Molecular Phylogenetic Analysis ◽  
Species Status ◽  
Separate Species ◽  
Female Ratio ◽  
Lateral Field ◽  
Female Tail ◽  
Molecular Phylogenetic ◽  
Males And Females

Abstract Bursaphelenchus paraparvispicularis n. sp. is described and figured from pine packaging wood originating in Hongkong, China, and inspected in Ningbo harbour, China. The new species clearly belongs to the hofmanni group. It is characterised by a relatively stout body (a = 26.7 and 26.5 for males and females, respectively), three lines in the lateral field, seven caudal papillae, spicules relatively small (12.6-15.3 μm), mitten-shaped, with lamina dorsal line smoothly arcuate but calomus relatively straight, condylus squared or round, well developed, rostrum well developed with round terminus, cucullus absent, the shape of the female tail, which is short and ventrally bent with a bluntly pointed terminus, and vulval lips not forming a vulval flap. The new species is morphologically closest to B. parvispicularis and can be distinguished by smaller and stouter body, lower female ratio c′ (average 2.8 vs 4.4) and longer spicule condylus. The separate species status is supported by ITS-RFLP patterns and molecular phylogenetic analysis based on ITS1/2 and partial LSU sequences, which revealed that B. paraparvispicularis n. sp. is closest to B. parvispicularis.

Description of Bursaphelenchus yuyaoensis n. sp. (Nematoda: Aphelenchoididae) isolated from Pinus massoniana in China

Nematology ◽  
2014 ◽  
Vol 16 (4) ◽  
pp. 411-418 ◽  
Author(s):  
Jianfeng Gu ◽  
Jie He ◽  
Jiangling Wang ◽  
Xianfeng Chen
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Pinus Massoniana ◽  
Species Status ◽  
Separate Species ◽  
Lateral Field ◽  
Male Tail ◽  
Female Tail ◽  
Molecular Phylogenetic ◽  
Males And Females

Bursaphelenchus yuyaoensisn. sp. is described and figured from dyingPinus massonianain Yuyao, China. The new species clearly belongs to thehofmannigroup. It is characterised by relatively slim body (a = 43.7 and 36.2 for males and females, respectively), three lines in the lateral field, spicules relatively small (11.4-14.7 μm), mitten-shaped with the posterior third of the dorsal limb distinctly curved, condylus round and slightly dorsally bent, rostrum well developed with more or less rounded terminus, distal ends of spicules not forming a clear cucullus, male tail with a mucron-like process, female tail slightly ventrally bent with a bluntly rounded terminus, vulval lip not forming a vulval flap and post-uterine sac extending for about half the vulva-anus distance. The separate species status is supported by ITS-RFLP patterns and molecular phylogenetic analysis based on ITS1/2 sequences, which revealed thatB. yuyaoensisn. sp. is close toB. parvispicularisandB. paraparvispicularis.

Description of Bursaphelenchus arthuroides sp. n. (Nematoda: Aphelenchoididae), a second parthenogenetic species of Bursaphelenchus Fuchs, 1937

Nematology ◽  
2012 ◽  
Vol 14 (1) ◽  
pp. 51-63 ◽  
Author(s):  
Jianfeng Gu ◽  
Jiangling Wang ◽  
Jingwu Zheng
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Molecular Phylogenetic Analysis ◽  
Dorsal Part ◽  
Species Status ◽  
Lateral Field ◽  
Middle Position ◽  
Parthenogenetic Species ◽  
Female Tail ◽  
Molecular Phylogenetic

Bursaphelenchus arthuroides sp. n. is described and figured from pine packaging wood originating in Brazil, and inspected in Ningbo harbour, P. R. China. The new species clearly belongs to the fungivorus group. It is characterised by four lines in the lateral field; spicules medium sized (15-17 μm), dorsal part distinctly sclerotised, with a high rounded condylus and a blunted pointed rostrum in the middle position, capitulum with a shallow depression, the posterior third of the dorsal limb ventrally bent, distal end broadly rounded without cucullus; the shape of the female tail conical and slim, posterior third distinctly ventrally bent with a finely pointed terminus; vulval lips slightly protruding but not forming a vulval flap and post-uterine sac extending for about half the vulva-anus distance. Species status is supported by ITS-RFLP patterns and molecular phylogenetic analysis based on partial LSU sequences, ITS1/2 and partial LSU sequences, which revealed that B. arthuroides sp. n. is closest to B. arthuri. Propagation tests also confirmed that the new species is parthenogenetic.

Description of Ruehmaphelenchus digitulus sp. n. (Nematoda: Aphelenchoididae) found in packaging wood from Taiwan

Nematology ◽  
2012 ◽  
Vol 14 (4) ◽  
pp. 489-498 ◽  
Author(s):  
Jianfeng Gu ◽  
Jiangling Wang
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Rflp Pattern ◽  
Species Status ◽  
Separate Species ◽  
Lateral Field ◽  
Male And Female ◽  
Female Tail ◽  
Molecular Phylogenetic ◽  
Shape And Size

Ruehmaphelenchus digitulus sp. n., isolated from coniferous packaging wood from Taiwan, is described and figured. The new species is characterised by a relatively stout body with a = 28.0 (23.7-31.3) and 27.0 (22.9-32.7) for male and female, respectively, three lines in the lateral field, spicules relatively small (chord = 17.0-19.2 μm; arc = 13.8-16.6 μm) with weakly developed condylus and rostrum, bursa absent, vulva positioned at ca 80% of body length, vulva flap absent, vulval lips slightly protruding, post-uterine branch extending slightly over half of vulva to anus distance, and tail conoid, ca 2-3 anal body diam. long, with terminal process 7-10 μm long. The new species is the fourth known for the genus. It can be distinguished from R. martinii, R. asiaticus and R. thailandae mainly by the different shape and size of the spicules and the different shape and length of the female tail terminus. The separate species status is also supported by ITS-RFLP pattern and molecular phylogenetic analysis based on partial SSU ITS1/2 and partial LSU sequences.

Description and phylogeny of Bursaphelenchus luxuriosae n. sp. (Nematoda: Aphelenchoididae) isolated from Acalolepta luxuriosa (Coleoptera: Cerambycidae)

Nematology ◽  
2003 ◽  
Vol 5 (4) ◽  
pp. 565-572 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Kazuyoshi Futai
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Botrytis Cinerea ◽  
Bursaphelenchus Xylophilus ◽  
Lateral Field ◽  
Female Tail ◽  
Phylogenetic Status ◽  
Rflp Profile ◽  
Molecular Phylogenetic ◽  
Males And Females

AbstractBursaphelenchus luxuriosae n. sp. is described and figured. Specimens were collected from a 2-week-old culture on Botrytis cinerea. The new species is characterised by a body length of 897 (710-1159) μm in the female and 745 (621-887) μm in the male, relatively robust body (a = 33-39 in the female and 27-30 in the male), stylet ca 14 (11-16) μm long, four lines in the lateral field, the large (27-30 μm) arcuate spicule with a terminal cucullus, seven (2 + 1 + 2 + 2) male caudal papillae, the long, well developed vulval flap and the shape of the female tail which is long, tapered, and ventrally bent when killed by heat with an irregular or roughened dorsal contour near the tip and an irregular terminus. The new species is considered to belong to the Bursaphelenchus xylophilus group of the genus Bursaphelenchus and is most closely related to B. conicaudatus and B. fraudulentus in spicule shape, vulval flap and 'a' values of males and females. It is easily distinguished from these two species by the morphology of female tail. The RFLP profile confirms the distinctness of the new species within the B. xylophilus group. The phylogenetic status of B. luxuriosae n. sp. within the B. xylophilus group is indicated by molecular phylogenetic analysis. Bursaphelenchus luxuriosae n. sp. is assumed to be close to B. conicaudatus and to have diverged from the ancestor of the B. xylophilus group early in the speciation of the group.

Description of Ruehmaphelenchus thailandae n. sp. (Nematoda: Aphelenchoididae) found in dunnage from Thailand

Nematology ◽  
2010 ◽  
Vol 12 (6) ◽  
pp. 869-876 ◽  
Author(s):  
Jianfeng Gu ◽  
Jiangling Wang
Keyword(s):  
New Species ◽  
Body Length ◽  
Rflp Pattern ◽  
Species Status ◽  
Separate Species ◽  
Lateral Field ◽  
The Third ◽  
Female Tail ◽  
Males And Females ◽  
Shape And Size

AbstractRuehmaphelenchus thailandae n. sp., isolated from deciduous dunnage from Thailand, is described and figured. The new species is characterised by the relatively stout body (a = 28.6 and 27.6 for males and females, respectively), three lines in the lateral field, spicules relatively small (12.0-13.3 μm) with weakly developed condylus and rostrum, bursa absent, vulva located at 79.5% of body length, vulval lips slightly swollen, length of post-uterine branch more than half of vulva to anus distance, female tail conoid, ca two anal body diam. long and with a broad terminal process up to 12 μm long. The new species is the third known for the genus. It can be distinguished from R. martinii and R. asiaticus mainly by the different shape and size of its spicules and different shape of the female tail terminus. The separate species status is also supported by ITS-RFLP pattern. Additional measurements of paratype R. asiaticus are appended.

Description of Ektaphelenchus ibericus n. sp. (Nematoda: Ektaphelenchinae) found in packaging wood from Spain

Nematology ◽  
2013 ◽  
Vol 15 (7) ◽  
pp. 871-878 ◽  
Author(s):  
Jianfeng Gu ◽  
Jiangling Wang ◽  
Xianfeng Chen ◽  
Xuan Wang
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Molecular Phylogenetic Analysis ◽  
Species Status ◽  
Separate Species ◽  
Lateral Lines ◽  
Molecular Phylogenetic

Ektaphelenchus ibericus n. sp. is described and figured. The new species was isolated from pine packaging wood from Spain that was inspected in Ningbo harbour, China, in 2011. Ektaphelenchus ibericus n. sp. is characterised by lip region without clear constriction, relatively short stylet (11-15 μm) lacking basal knobs, and four lateral lines. The female post-uterine sac is less than one body diam. long, rectum and anus are absent, and the tail is conoid with a sharply pointed terminus. Males are rare and have a conoid tail with a sharply pointed terminus, spicules mitten-shaped, 12.8 μm long (chord) and smoothly curved with a high and bluntly rounded condylus and bluntly pointed rostrum, distal end broadly rounded, and three pairs of ventral caudal papillae present. The separate species status is also supported by molecular phylogenetic analysis based on partial SSU, ITS1/2 and partial LSU sequences.

Peponium elgonense (Cucurbitaceae), a new species from Mount Elgon in Kenya

Phytotaxa ◽  
2020 ◽  
Vol 443 (2) ◽  
pp. 189-197
Author(s):  
FREDRICK MUNYAO MUTIE ◽  
PENINAH CHEPTOO RONO ◽  
VIVIAN KATHAMBI ◽  
NENG WEI ◽  
PERIS KAMAU ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Molecular Phylogenetic Analysis ◽  
Trnl Intron ◽  
Separate Species ◽  
Molecular Phylogenetic ◽  
Plastid Markers ◽  
Mount Elgon ◽  
A New Species

Peponium elgonense, a new species of Cucurbitaceae from Mount Elgon in Kenya, is described and illustrated here with photographs. This species is similar to P. cienkowskii and P. vogelii, but differs by being monoecious and having a longer ovary. A molecular phylogenetic analysis of Peponium based on ITS and five plastid markers (trnL–F, rpl20–rps12, matK, rbcL, and trnL intron) of four species of Peponium further shows that P. elgonense individuals cluster together supporting the argument to regard it as a separate species.

Pilostyles maya, a novel species from Mexico and the first cleistogamous species in Apodanthaceae (Cucurbitales)

Phytotaxa ◽  
2020 ◽  
Vol 440 (4) ◽  
pp. 255-267
Author(s):  
PACTLI F. ORTEGA-GONZÁLEZ ◽  
SANDRA RIOS-CARRASCO ◽  
CÉSAR ADRIÁN GONZÁLEZ-MARTÍNEZ ◽  
NATALIA BONILLA-CRUZ ◽  
SONIA VÁZQUEZ-SANTANA
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Novel Species ◽  
Critically Endangered ◽  
Molecular Phylogenetic Analysis ◽  
Morphological Evidence ◽  
Species Status ◽  
New Mexican ◽  
Molecular Phylogenetic ◽  
Mexican Species

Pilostyles maya (Apodanthaceae), a new Mexican species endemic to the Yucatán Peninsula, is described and illustrated. Ecological, geographical, molecular, and morphological evidence are provided to support the new species status of Pilostyles maya. A molecular phylogenetic analysis based on the mitochondrial matR and nuclear 18S genes recovered P. maya as sister to P. mexicana. It is distinguished from its congeners by having cleistogamous, hermaphroditic flowers, tepals with ciliate margins and specificity on a Bauhinia host. These traits are novel for the genus and family. We propose that P. maya be listed as Critically Endangered due to its small known area of distribution and threats from deforestation in its area of occurrence.

Description of Ruehmaphelenchus taedae n. sp. (Nematoda: Aphelenchoididae) found in Loblolly pine logs from the USA

Nematology ◽  
2021 ◽  
pp. 1-10
Author(s):  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Xinxin Ma ◽  
Xiaoling Lü ◽  
Xianfeng Chen
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Loblolly Pine ◽  
Excretory Pore ◽  
Lateral Field ◽  
Female Tail ◽  
The Status ◽  
The Usa ◽  
Males And Females ◽  
A New Species

Summary Ruehmaphelenchus taedae n. sp., isolated from Loblolly pine logs (Pinus taedae L.) from the USA, is described and figured. It is characterised by a relatively slim body (a = 42 and 43 for males and females, respectively), three lines in the lateral field, male spicules relatively small (12-18 μm) with high and dorsally bent condylus and weakly developed rostrum, bursal flap absent, short tail possessing a long terminal spike ending in a bluntly rounded tip and 8.7-13.3 μm long, vulva positioned at ca 83% of body length, vulval flap absent, vulval lips slightly protruding, post-vulval uterine branch extending for less than half of vulva to anus distance, and female tail conoid, ca 3-4 anal body diam. long, with 13.7-18.5 μm terminal projection. The new species can be separated from all other species of the genus by the male tail possessing a long terminal spike and the more anterior excretory pore. Detailed phylogenetic analysis based on 28S D2-D3 region sequences confirmed the status of this nematode as a new species.

Description of Ektaphelenchoides spondylis n. sp. (Nematoda: Ektaphelenchinae) isolated from Spondylis buprestoides (Coleoptera: Cerambycidae) in Japan

Nematology ◽  
2009 ◽  
Vol 11 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Robin Giblin-Davis ◽  
Barbara Center
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Large Subunit ◽  
Taxonomic Revision ◽  
Molecular Phylogenetic Analysis ◽  
Molecular Sequence ◽  
Male Ratio ◽  
Female Tail ◽  
Tail Shape ◽  
Molecular Phylogenetic

AbstractEktaphelenchoides spondylis n. sp. is described and figured from Spondylis buprestoides. The new species is characterised by the bipartite stylet conus, male spicule with rounded condylus, blunt rostrum and plate-like cucullus, long postuterine sac and long and smoothly tapering female tail. The new species is almost morphologically identical to E. compsi, but can be distinguished from it by stylet morphology, female tail shape, male ratio (a) and molecular sequence of the D2/D3 expansion segment of the large subunit of ribosomal DNA. In the molecular phylogenetic analysis, E. spondylis n. sp. formed a well supported clade with E. compsi within Ektaphelenchoides. Based upon molecular phylogenetic analysis with one locus, Ektaphelenchoides spp. formed a well supported clade and Ektaphelenchus and Cryptaphelenchus also formed a well supported clade. However, the molecular and morphological information available from our study are insufficient to revise the generic taxonomy of the subfamily. More sampling and an integrated taxonomic revision of the subfamily Ektaphelenchinae are needed.

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