Cacopsylla fraudatrix sp.n. (Hemiptera: Psylloidea) recognised from testis structure and mitochondrial gene COI

Zootaxa ◽  
2012 ◽  
Vol 3547 (1) ◽  
pp. 55 ◽  
Author(s):  
VALENTINA G. KUZNETSOVA ◽  
EUGENIA S. LABINA ◽  
NAZAR A. SHAPOVAL ◽  
ANNA MARYAŃSKA-NADACHOWSKA ◽  
VLADIMIR A. LUKHTANOV
Keyword(s):  
Related Species ◽  
Mitochondrial Gene ◽  
Coi Gene ◽  
Nucleotide Substitutions ◽  
Closely Related Species ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Molecular Phylogenetic ◽  
Gene Coi ◽  
Testis Structure

Cacopsylla fraudatrix Labina & Kuznetsova sp.n., collected from Vaccinium myrtillus in Bieszczady Mountains (S. Poland) is described and compared with the most closely related species, C. myrtilli (developing on V. myrtillus) and C. ledi (on Ledum palustre) as well as with a distantly related species C. vaccinii  (on V. bracteatum). The identity of the new species is supported by the structure of testes consisting of two follicles each, in contrast to four in C. myrtilli and C. ledi, and by a molecular phylogenetic analysis based on a 714 bp fragment of the mitochondrial COI gene. C. fraudatrix sp. n. forms a distinct clade in MP, ML and BI trees and differs from C. myrtilli by 28 fixed nucleotide substitutions (uncorrected p-distance = 3.92%) and from C. ledi by 26 fixed nucleotide substitutions (uncorrected p-distance = 3.64%).

scholarly journals A new subterranean Crematogaster with one ommatidium from Cambodia, based on morphology and DNA (Hymenoptera: Formicidae)

2019 ◽  
Vol 59 (2) ◽  
pp. 507-511
Author(s):  
Shingo Hosoishi
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Related Species ◽  
Coi Gene ◽  
Closely Related Species ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene

Abstract A subterranean myrmicine ant Crematogaster monocula sp. nov. is described based on two worker specimens from Cambodia. This species belongs to the C. biroi group of the subgenus Orthocrema Santschi, 1918, which is characterized by developed subpetiolar process, developed subpostpetiolar process and sculptured mesosoma. The new species is easily distinguished from other species of Crematogaster Lund, 1831 by having only one ommatidium. DNA sequences comprising a 761 bp fragment of the 3’ region of the mitochondrial COI gene were amplified and analyzed for C. monocula sp. nov. and other closely related species from the same group. The interspecific COI divergence is 17.8% for C. monocula sp. nov. and C. reticulata Hosoishi, 2009. This species is unique in having the smallest eye in the genus Crematogaster.

scholarly journals Ecological genetics of Adalia beetles: variability and symbiotic bacteria in european populations of the ten-spot ladybird beetle Adalia decempunctata

2019 ◽  
Vol 17 (4) ◽  
pp. 37-45
Author(s):  
Elena V. Shaikevich ◽  
Ilya A. Zakharov ◽  
Alois Honek
Keyword(s):  
Ecological Genetics ◽  
Coi Gene ◽  
Symbiotic Bacteria ◽  
Nucleotide Substitutions ◽  
Ladybird Beetle ◽  
Mitochondrial Coi ◽  
Glta Gene ◽  
Mitochondrial Coi Gene ◽  
Palearctic Species ◽  
Horizontal Transfers

Background. Adalia decempunctata L. (Coleoptera: Coccinellidae) ten-spot ladybird beetle, widespread morphologically variable Palearctic species. Materials and methods. DNA polymorphism and infection with Wolbachia, Spiroplasma and Rickettsia symbiotic bacteria were investigated. Results. Eight different haplotypes of the mitochondrial COI gene, seven of which were previously unknown, were found in 92 A. decempunctata individuals from nine European collection places: Prague, Rome, Florence, Hamburg, Paris, Stockholm, Moscow, Feodosia and Yalta. A. decempunctata is less variable in mtDNA compared to A. bipunctata. Symbiotic bacteria Wolbachia and Spiroplasma were not detected. Only Rickettsia infestation was found in A. decempunctata specimens, gathered in Stockholm and Feodosia. Rickettsia from A. decempunctata from Feodosia and Stockholm differ by 0.5% in gltA gene. Rickettsia from A. decempunctata from Feodosia is clustered with Rickettsia from A. bipunctata and Coccinella sp. based on the analysis of the gltA gene. Conclusion: Three of the eight mtDNA haplotypes are present in the A. decempunctata gene pool from geographically distant habitats. A small amount of nucleotide substitutions between Rickettsia from A. decempunctata and A. bipunctata suggests a single origin of the symbiont in the ladybirds of the genus Adalia, the results do not exclude subsequent horizontal transfers between individuals of both species.

scholarly journals DNA barcodes: Evaluating the potential of COI to diffentiate closely related species of Elachista (Lepidoptera: Gelechioidea: Elachistidae) from Australia

Zootaxa ◽  
2006 ◽  
Vol 1170 (1) ◽  
pp. 1 ◽  
Author(s):  
LAURI KAILA ◽  
GUNILLA STÅHLS
Keyword(s):  
Related Species ◽  
Coi Gene ◽  
Closely Related Species ◽  
Additional Tool ◽  
Putative Species ◽  
Species Complexes ◽  
Coi Barcoding ◽  
Gene Coi ◽  
Ecological Differences ◽  
Identification Tool

We compared DNA barcoding to “traditional” taxonomic tools in clarifying relationships in complexes of closely related, putative “species” of Elachistinae moths (Gelechioidea: Elachistidae) occurring in Australia. A 705 bp fragment of the 3’-end of cytochrome c oxidase subunit I gene (COI) was used. This mtDNA fragment did not differentiate between all species-level taxa that could be defined by morphological and/or ecological differences. Different evolutionary rates of COI among closely related lineages were observed. Although our findings are based on the variability of the 3’ end of the COI gene and not the 5’ end barcode fragment, we are convinced that thorough exploration of traditional morphology and ecology is a prerequisite for exploring insufficiently known taxonomies by the barcode approach. The sole use of COI barcoding, whether considering COI-5’ or COI-3’ fragment, may fail to recognize closely related species. Our results discourage this approach for delimitation of closely related species, but its use is encouraged as an additional tool for exploring little known taxonomies or as an identification tool for previously thoroughly studied species complexes.

A review of the genus Forficuloecus Conci (Phthiraptera: Philopteridae) from parrots (Psittaciformes: Psittacidae), with descriptions of four new species

Zootaxa ◽  
2008 ◽  
Vol 1859 (1) ◽  
pp. 49 ◽  
Author(s):  
ROGER D. PRICE ◽  
KEVIN P. JOHNSON ◽  
RICARDO L. PALMA
Keyword(s):  
New Species ◽  
Coi Gene ◽  
Chewing Louse ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Genetic Distinctiveness

Ten species, including four new species, of the chewing louse genus Forficuloecus Conci, 1941, are recognized from Australasian parrots, and a key is given for their identification. The new species and their type hosts are: F. cameroni ex the Red-winged Parrot, Aprosmictus erythropterus (J.F. Gmelin, 1788); F. banksi ex the Mulga Parrot, Psephotus varius Clark, 1910; F. wilsoni ex the Northern Rosella, Platycercus venustus (Kuhl, 1820); and F. josephi ex the Bourke's Parrot, Neopsephotus bourkii (Gould, 1841). Partial sequences of the mitochondrial COI gene support the genetic distinctiveness of these new species.

Are Tetranychus macfarlanei Baker & Pritchard and Tetranychus malaysiensis Ehara (Acari: Tetranychidae) one species? Morphological and molecular evidences for synonymy between these two spider mite species and a note on invasiveness of T. macfarlanei on okra and eggplant in India

2017 ◽  
Vol 22 (4) ◽  
pp. 467
Author(s):  
Mahran Zeity ◽  
Nagappa Srinivas ◽  
Chinnamade Channegowde Gowda
Keyword(s):  
Spider Mite ◽  
Morphological Characters ◽  
Molecular Data ◽  
Mite Species ◽  
Coi Gene ◽  
High Similarity ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
High Degree ◽  
Degree Of Similarity

Study of morphological characters of Tetranychus macfarlanei Baker & Pritchard and Tetranychus malaysiensis Ehara revealed high similarity by comparing all the important characters in addition to the characters pointed out by Ehara to separate those two species. Molecular phylogeny of seven Indian populations of T. macfarlanei and one population of T. malaysiensis from Philippines along with few distantly related species of Tetranychus was attempted. High degree of similarity between these two species at mitochondrial COI gene (96%) as well as ITS2 (rDNA) (96–99%) region was evident. Based on both morphological features and molecular data, T. malaysiensis is proposed as a junior synonym of T. macfarlanei based on ICZN’s law of priority. Also more female characters are prompted in this study to distinctly discriminate T. macfarlanei from its most resembling species, Tetranychus ludeni Zacher. Tetranychus macfarlanei has emerged as a pest of several cultivated crop plants in India. 

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