Are Tetranychus macfarlanei Baker & Pritchard and Tetranychus malaysiensis Ehara (Acari: Tetranychidae) one species? Morphological and molecular evidences for synonymy between these two spider mite species and a note on invasiveness of T. macfarlanei on okra and eggplant in India

2017 ◽  
Vol 22 (4) ◽  
pp. 467
Author(s):  
Mahran Zeity ◽  
Nagappa Srinivas ◽  
Chinnamade Channegowde Gowda
Keyword(s):  
Spider Mite ◽  
Morphological Characters ◽  
Molecular Data ◽  
Mite Species ◽  
Coi Gene ◽  
High Similarity ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
High Degree ◽  
Degree Of Similarity

Study of morphological characters of Tetranychus macfarlanei Baker & Pritchard and Tetranychus malaysiensis Ehara revealed high similarity by comparing all the important characters in addition to the characters pointed out by Ehara to separate those two species. Molecular phylogeny of seven Indian populations of T. macfarlanei and one population of T. malaysiensis from Philippines along with few distantly related species of Tetranychus was attempted. High degree of similarity between these two species at mitochondrial COI gene (96%) as well as ITS2 (rDNA) (96–99%) region was evident. Based on both morphological features and molecular data, T. malaysiensis is proposed as a junior synonym of T. macfarlanei based on ICZN’s law of priority. Also more female characters are prompted in this study to distinctly discriminate T. macfarlanei from its most resembling species, Tetranychus ludeni Zacher. Tetranychus macfarlanei has emerged as a pest of several cultivated crop plants in India. 

A new species of Carpenter Moths (Lepidoptera, Cossidae) from Tarbagatai (NE Kazakhstan) and Altai (SW Siberia, Russia) Mountains

Zootaxa ◽  
2020 ◽  
Vol 4896 (1) ◽  
pp. 85-95
Author(s):  
ROMAN V. YAKOVLEV ◽  
NAZAR A. SHAPOVAL ◽  
VADIM V. IVONIN ◽  
SVYATOSLAV A. KNYAZEV ◽  
GALINA N. KUFTINA ◽  
...  
Keyword(s):  
New Species ◽  
Male Genitalia ◽  
Molecular Data ◽  
Coi Gene ◽  
Wing Pattern ◽  
Altai Mountains ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
A New Species

We described a new cossid species, Dyspessa ulgen sp. nov. from the Tarbagatai and Altai Mountains and compared it to other taxa of Dyspessa reported from the region (D. tristis, D. saldaitisi, D. saissanica), as well as to morphologically similar D. ulula. The new species is most closely related to D. ulula but differs from the latter in the characteristics of the male genitalia, wing pattern, and molecular data (a 658 bp fragment of the mitochondrial COI gene). 

Description of a new species of the genus Maua Distant (Hemiptera, Cicadidae) from China

Zootaxa ◽  
2021 ◽  
Vol 4949 (3) ◽  
pp. 582-588
Author(s):  
JIALI WANG ◽  
JOANNES P. DUFFELS ◽  
CONG WEI
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Intraspecific Variation ◽  
Male Genitalia ◽  
Morphological Characters ◽  
Coi Gene ◽  
Morphological Observation ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
A New Species

A new species, Maua squeala sp. nov., is described from China. This species is similar to M. affinis Distant, 1905 and M. palawanensis Duffels, 2009, but can be distinguished by the shorter and more slender body of the new species, the lateral fasciae on the mesonotum and the shape of the male genitalia. The intraspecific variation of this species is discussed based on morphological observation combined with sequences of partial mitochondrial COI gene (DNA barcoding) of individuals exhibiting different morphological characters. 

scholarly journals Identification of Natural Hybrids between Ahlbergia frivaldszkyi (Lederer, 1853) and Callophrys rubi (Linnaeus, 1758) (Lepidoptera, Lycaenidae) Using Mitochondrial and Nuclear Markers

Insects ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1124
Author(s):  
Nazar A. Shapoval ◽  
Roman V. Yakovlev ◽  
Galina N. Kuftina ◽  
Vladimir A. Lukhtanov ◽  
Svyatoslav A. Knyazev ◽  
...  
Keyword(s):  
Morphological Characters ◽  
Natural Hybridization ◽  
Hybrid Origin ◽  
Coi Gene ◽  
Genetic Introgression ◽  
Mitochondrial Coi ◽  
Mitochondrial Haplotypes ◽  
Phylogenetic Studies ◽  
Mitochondrial Coi Gene ◽  
Evolutionary Consequences

Natural hybridization is rather widespread and common in animals and can have important evolutionary consequences. In terms of taxonomy, exploring hybridization and introgression is crucial in defining species boundaries and testing taxonomic hypotheses. In the present paper, we report on natural hybrid specimens between Ahlbergia frivaldszkyi (Lederer, 1853) and Callophrys rubi (Linnaeus, 1758). To test the hypothesis of their hybrid origin, we employed the molecular mitochondrial (COI gene) and nuclear (wingless, RPS5, and Ca-ATPase genes) markers commonly used in phylogenetic studies and explored the morphology of the specimens. Our analysis revealed that hybrids bear mitochondrial haplotypes of C. rubi, while nuclear fragments are heterozygous, sharing a combination of A. frivaldszkyi and C. rubi lineages. The hybrid specimens combine morphological characters of both genera. Our results for the first time empirically demonstrate the possibility of genetic introgression between these species and between the genera Callophrys and Ahlbergia on the whole.

Pilumnus Leach (Decapoda:Pilumnidae Samouelle) from Mediterranean and adjacent Atlantic waters: a COI analysis

2010 ◽  
Vol 24 (2) ◽  
pp. 182 ◽  
Author(s):  
Ulla Oliveira-Biener ◽  
Roland R. Melzer ◽  
Michael A. Miller
Keyword(s):  
Atlantic Ocean ◽  
Mediterranean Sea ◽  
Base Pair ◽  
Molecular Taxonomy ◽  
Morphological Characters ◽  
The Other ◽  
Coi Gene ◽  
Mediterranean Species ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene

A molecular taxonomy is presented based on 544 base pair sequences of the mitochondrial COI gene (Palumbi segment) from 141 bristle crabs of the genus Pilumnus from the Mediterranean Sea and adjacent Atlantic Ocean, plus outgroups. The sample includes all currently valid autochthonous Mediterranean species. Six well-supported COI lineages were detected, three of which exclusively comprise representatives of Pilumnus inermis, P. spinifer and P. villosissimus, respectively. On the other hand, crabs that would be attributed to P. hirtellus according to current morphology-based taxonomy were distributed over three COI clades, two of which are interpreted as representing cryptic lineages. We compare our findings to previous analyses using sets of external morphological characters, and discuss the partial mismatch between the morphology- and gene-based classifications.

Fixation, description and DNA barcode of a neotype for Botryllus schlosseri (Pallas, 1766) (Tunicata, Ascidiacea)

Zootaxa ◽  
2017 ◽  
Vol 4353 (1) ◽  
pp. 29 ◽  
Author(s):  
RICCARDO BRUNETTI ◽  
LUCIA MANNI ◽  
FRANCESCO MASTROTOTARO ◽  
CARMELA GISSI ◽  
FABIO GASPARINI
Keyword(s):  
Developmental Stages ◽  
Dna Barcode ◽  
Molecular Data ◽  
Coi Gene ◽  
Morphological Description ◽  
Botryllus Schlosseri ◽  
Colonial Ascidian ◽  
History Museum ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene

Botryllus schlosseri is a widespread colonial ascidian commonly considered cosmopolitan and amply used as model for researches ranging from developmental biology to immunobiology. Recently, molecular data lead to hypothesize that the species named B. schlosseri may consist of more than a single taxon. Indeed, five highly divergent clades, named A-E, have been genetically identified and are referred as cryptic species. In this context, and lacking both a type and a detailed morphological description, we believe that it is necessary, as a taxonomic reference point, to designate a neotype and re-describe the species. Therefore, a sample from the Lagoon of Venice (Adriatic Sea, Italy) was deposited as neotype in the Natural History Museum of Venice (Italy), preserved both in formalin and in 90% ethanol. Here we provide a morphological description of the suggested neotype of B. schlosseri that takes into account several developmental stages (oozooid, zooid of first blastogenic generations, and mature zooid) and is carefully compared with the previous descriptions of samples coming from other European and non-European localities. Finally, we associate our morphological description to a “DNA barcode”, consisting in a long fragment of the mitochondrial COI gene. Our description is associated to clade A, although at now we cannot guarantee that this association is univocal.

scholarly journals The discovery of Scutovertex ianus sp. nov. (Acari, Oribatida) – a combined approach of comparative morphology, morphometry and molecular data

2010 ◽  
Vol 79 (1) ◽  
pp. 39-55
Author(s):  
Tobias Pfingstl ◽  
Sylvia Schäffer ◽  
Ernst Ebermann ◽  
Günther Krisper
Keyword(s):  
New Species ◽  
Oribatid Mite ◽  
Intraspecific Variability ◽  
Comparative Morphology ◽  
Molecular Data ◽  
Mite Species ◽  
Coi Gene ◽  
Separate Species ◽  
Mitochondrial Coi ◽  
Molecular Phylogenetic

Based on morphological, morphometric and genetic data Scutovertex ianus sp. nov. is described as a new oribatid mite species. The traditional comparison with the morphologically most similar congeneric S. minutus and S. sculptus demonstrated that the new species shares certain characters with both species, but can be clearly identified by indistinct cuticular notogastral foveae in combination with short spiniform notogastral setae. Furthermore the eggs of S. ianus exhibit a different fine structure of the exochorion. The morphometric analysis of 16 continuous morphological variables separated the three species, S. minutus, S. sculptus and S. ianus with a certain overlap indicating minor size and shape differences in overall morphology. The molecular phylogenetic analysis of mitochondrial COI gene sequences supported the monophyly of all three investigated species and confirmed S. ianus as separate species with high bootstrap values. Each performed analysis approves the discreteness of S. ianus and the results contradict the formerly supposed large intraspecific variability of the representatives of the genus Scutovertex. The records of S. ianus are as yet restricted to the Eastern part of Austria and to one location in Germany, but findings of intermediary Scutovertex specimens from other European countries may refer to this new species.

scholarly journals Taxonomy and phylogenetic relationships of the coral genera Australomussa and Parascolymia (Scleractinia, Lobophylliidae)

2014 ◽  
Vol 83 (3) ◽  
pp. 195-S7 ◽  
Author(s):  
Roberto Arrigoni ◽  
Zoe T. Richards ◽  
Chaolun Allen Chen ◽  
Andrew H. Baird ◽  
Francesca Benzoni
Keyword(s):  
Histone H3 ◽  
Taxonomic Status ◽  
Its Region ◽  
Molecular Data ◽  
Coi Gene ◽  
Phylogenetic Position ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
The Family ◽  
Micromorphological Characters

Novel micromorphological characters in combination with molecular studies have led to an extensive revision of the taxonomy and systematics of scleractinian corals. In the present work, we investigate the macro- and micromorphology and the phylogenetic position of the genera Australomussa and Parascolymia, two monotypic genera ascribed to the family Lobophylliidae. The molecular phylogeny of both genera was addressed using three markers, the partial mitochondrial COI gene and the nuclear histone H3 and the ribosomal ITS region. Based on molecular data, Australomussa and Parascolymia belong to the Lobophylliidae and they cluster together with the genera Lobophyllia and Symphyllia within the same clade. While A. rowleyensis and P. vitiensis are closely related based on the three gene regions examined, their macro and micromorphology suggest that these species are distinct, differing in several characters, such as continuity and thickness of the costosepta, the number of septa, septal tooth height, spacing, and shape, and the distribution and shape of granules. Thus, we revise the taxonomic status of the genus Australomussa as a junior synonym of Parascolymia.

scholarly journals Molecular data confirm the presence of Nycticebus bengalensis on Langkawi Island, Malaysia

2019 ◽  
Vol 20 (4) ◽  
pp. 1115-1120
Author(s):  
BADRUL MUNIR MD ZAIN ◽  
KHAIRUL SYA’ADAH MOHHOYUA ◽  
NOR RAHMAN AIFAT ◽  
EHWAN NGADI ◽  
NORSHAQINAH AYOB ◽  
...  
Keyword(s):  
Significant Contribution ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Peninsular Malaysia ◽  
Coi Gene ◽  
Slow Loris ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Tree Topologies ◽  
High Bootstrap

Abstract. Md-Zain BM, Mohhoyua KS, Aifat NR, Ngadi E, Ayob N, Rovie-Ryan JJ, Ampeng A, Mohd-Ridwan AR, Blair ME, Abdul-Latiff MAB. 2019. Molecular data confirm the presence of Nycticebus bengalensis on Langkawi Island, Malaysia. Biodiversitas 20: 1115-1120. Recent taxonomic reviews have stated the possibility of Bengal Slow Loris (Nycticebus bengalensis) presence in the Northern part of the Malay Peninsula. This study aims to confirm the presence of the Bengal Slow Loris in Malaysia by sequencing the mitochondrial COI gene from samples collected from Langkawi Island, Peninsular Malaysia, and Borneo. Phylogenetic analyses produced tree topologies that support the grouping of slow loris samples by their localities. The tree topologies further show that slow loris samples from Sarawak and Peninsular Malaysia form two distinct clades. The clade from Peninsular Malaysia was divided into two subclades, Langkawi and Selangor. The Langkawi slow loris subclade includes sequences from GenBank representing N. bengalensis, supported by a high bootstrap value. This mitochondrial DNA finding has a significant contribution to indicate the presence of the Bengal Slow Loris in Malaysia.

Identification of a species of deer fly attacking human and live stocks in Assam, India

ENTOMON ◽  
2019 ◽  
Vol 44 (3) ◽  
pp. 237-240
Author(s):  
Dipankar Biswas ◽  
Dibya Ranjan Bhattacharyya ◽  
Kaushal Yadav ◽  
Pranjal Jyoti Baruah ◽  
Biswa Jyoti Borkakoty
Keyword(s):  
Present Article ◽  
Sequence Data ◽  
Molecular Data ◽  
Northeast India ◽  
Species Level ◽  
Coi Gene ◽  
Mitochondrial Coi ◽  
Preliminary Examination ◽  
Mitochondrial Coi Gene ◽  
Sudden Appearance

During April 2018, a sudden appearance of some unknown flies was observed in some villages under Sibsagar district of Assam, Northeast India. The flies attacked in groups and had more attraction towards human than livestock. The present article describes the entomological and molecular identification of the fly at species level in Sibsagar district of Assam, northeast India. Preliminary examination revealed the fly to be an insect of order Diptera, Family Tabanidae and Genus Chrysops as they posses short proboscis, ocelli, third antennal joint with five divisions and wings demarcated with dark median cross-band. This was supported by molecular data where the partial nucleotide sequences of Mitochondrial COI gene revealed maximum identity (90.6%-92.3%) with genus Chrysops. The mitochondrial COI sequence data of Chrysops flavocinctus has been made available in NCBI Gen Bank. Gen Bank Accession No. MH998226.

scholarly journals Just the once will not hurt: DNA suggests species lumping over two oceans in deep-sea snails (Cryptogemma)

2020 ◽  
Vol 190 (2) ◽  
pp. 532-557 ◽  
Author(s):  
Paul Zaharias ◽  
Yuri I Kantor ◽  
Alexander E Fedosov ◽  
Francesco Criscione ◽  
Anders Hallan ◽  
...  
Keyword(s):  
Deep Sea ◽  
Species Delimitation ◽  
Molecular Data ◽  
Shell Morphology ◽  
Coi Gene ◽  
Type Specimens ◽  
Mitochondrial Coi ◽  
Species Complexes ◽  
Mitochondrial Coi Gene ◽  
Taxonomic Studies

Abstract The practice of species delimitation using molecular data commonly leads to the revealing of species complexes and an increase in the number of delimited species. In a few instances, however, DNA-based taxonomy has led to lumping together of previously described species. Here, we delimit species in the genus Cryptogemma (Gastropoda: Conoidea: Turridae), a group of deep-sea snails with a wide geographical distribution, primarily by using the mitochondrial COI gene. Three approaches of species delimitation (ABGD, mPTP and GMYC) were applied to define species partitions. All approaches resulted in eight species. According to previous taxonomic studies and shell morphology, 23 available names potentially apply to the eight Cryptogemma species that were recognized herein. Shell morphometrics, radular characters and geographical and bathymetric distributions were used to link type specimens to these delimited species. In all, 23 of these available names are here attributed to seven species, resulting in 16 synonymizations, and one species is described as new: Cryptogemma powelli sp. nov. We discuss the possible reasons underlying the apparent overdescription of species within Cryptogemma, which is shown here to constitute a rare case of DNA-based species lumping in the hyper-diversified superfamily Conoidea.

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