Diagnostic and phylogenetic utility of the first DNA barcode library for longhorn beetles (Coleoptera: Cerambycidae) from the Russian Far East

Zootaxa ◽  
2017 ◽  
Vol 4276 (3) ◽  
pp. 441 ◽  
Author(s):  
VASILY V. GREBENNIKOV ◽  
EDUARD JENDEK ◽  
MAXIM ED. SMIRNOV
Keyword(s):  
Dna Barcoding ◽  
Phylogenetic Signal ◽  
Russian Far East ◽  
Far East ◽  
Dna Barcode ◽  
Longhorn Beetles ◽  
Gene Coi ◽  
Online Library ◽  
Identification Tool ◽  
The Russian Far East

One hundred forty two specimens representing 56 species of longhorn beetles (Cerambycidae) from the Russian Far East were sequenced for a 658 bp fragment of the 5' end of the mitochondrial cytochrome oxidase subunit I gene (COI, =DNA barcode). The data are publicly available in an open access online library (dx.doi.org/10.5883/DS-CERRF). All analysed species could be differentiated using the standard online DNA barcode identification tool, except for a group of three sympatric colour-defined Menesia species. Seven Menesia records share the same Barcode Identification Number (=BIN), while the single specimen of M. flavotecta shares the same haplotype as some of M. sulphurata. Excluding the Menesia case, the Barcode Index Numbers (BINs) uniquely correspond to the analysed species, in all but the four specimens of Chlorophorus simillimus where they share two BINs. Although DNA barcoding aims to develop species identification systems, some phylogenetic signal was apparent in the data and in the Maximum Likelihood analysis, all four subfamilies were recovered as monophyletic. Notwithstanding the few detected deviations from the absolute taxonomic/phylogenetic match, DNA barcoding is a powerful identification tool with a capacity to place an undocumented record among its closest relatives. 

scholarly journals DNA-barcoding and a new data about the karyotype of Myotis petax (Chiroptera, Vespertilionidae) in the Russian Far East

2020 ◽  
Vol 14 (4) ◽  
pp. 483-500
Author(s):  
Uliana V. Gorobeyko ◽  
Irina V. Kartavtseva ◽  
Irina N. Sheremetyeva ◽  
Denis V. Kazakov ◽  
Valentin Yu. Guskov
Keyword(s):  
Dna Barcoding ◽  
Russian Far East ◽  
Far East ◽  
Intraspecific Variability ◽  
Diagnostic Feature ◽  
Nucleotide Variability ◽  
The Family ◽  
Coi Barcoding ◽  
First Time ◽  
The Russian Far East

The DNA-barcoding and chromosomal study of the eastern water bat, Myotis petax Hollister, 1912, from the earlier unexplored localities in the Russian Far East are carried out. The COI barcoding obtained for 18 from a total of 19 individuals captured in five localities in the Russian Far East showed the low nucleotide variability with the prevalence of the central, the most abundant haplotype. The chromosomal characteristics of eight M. petax specimens (2n = 44, NFa = 52) in the Russian Far East are clarified. The number and localization of NOR in karyotype of M. petax is described at the first time and differ from distributional patterns of NOR in the sibling species M. daubentonii Kuhl, 1819 that can be used as diagnostic feature. The considerable intraspecific variability in the distribution of heterochromatin material revealed is not typical of the genus Myotis, but it has been found in other species of the family Vespertilionidae.

Exploring species diversity and host plant associations of leaf-mining micromoths (Lepidoptera: Gracillariidae) in the Russian Far East using DNA barcoding

Zootaxa ◽  
2019 ◽  
Vol 4652 (1) ◽  
pp. 1-55 ◽  
Author(s):  
NATALIA KIRICHENKO ◽  
PAOLO TRIBERTI ◽  
EVGENIY AKULOV ◽  
MARGARITA PONOMARENKO ◽  
SVETLANA GOROKHOVA ◽  
...  
Keyword(s):  
Host Plant ◽  
Dna Barcoding ◽  
Russian Far East ◽  
Far East ◽  
Betula Platyphylla ◽  
Primorskii Krai ◽  
Plant Associations ◽  
Leaf Mining ◽  
First Time ◽  
The Russian Far East

The Russian Far East (RFE) is an important hotspot of biodiversity whose insect fauna remains understudied, particularly its Microlepidoptera. Here we explore the diversity of leaf-mining micromoths of the family Gracillariidae, their distribution and host plant associations in RFE using a combination of field observations and sampling, DNA barcoding, morphological analysis and literature review.                We collected 91 gracillariid specimens (45 larvae, 9 pupae and 37 adults) in 12 localities across RFE and identified 34 species using a combination of DNA barcoding and morphology. We provide a genetic library of 57 DNA barcodes belonging to 37 Barcode Index Numbers (BINs), including four BINs that could potentially represent species new to science. Leaf mines and leaf shelters are described and illustrated for 32 studied species, male or female genitalia as well as forewing patterns of adults are shown, especially for those species identified based on morphology.                Three species, Micrurapteryx caraganella (Hering), Callisto insperatella (Nickerl), and Phyllonorycter junoniella (Zeller) are newly recorded from RFE. Five species previously known from some regions of RFE, were found for the first time in Amurskaya Oblast: Phyllonorycter populifoliella (Treitschke), Primorskii Krai: Ph. sorbicola Kumata and Sahkalin Island: Caloptilia heringi Kumata, Ph. ermani (Kumata) and Ph. ulmifoliella (Hübner). Eight gracillariid–plant associations are novel to science: Caloptilia gloriosa Kumata on Acer pseudosieboldianum, Cameraria niphonica Kumata on A. caudatum subsp. ukurundense, Parornix ermolaevi Kuznetzov on Corylus sieboldiana, Phyllonorycter ermani (Kumata) on Betula platyphylla, Ph. nipponicella (Issiki) on Quercus mongolica, Ph. orientalis (Kumata) and Ph. pseudojezoniella Noreika on Acer saccharum, Ph. sorbicola on Prunus maakii. For the first time we documented the “green island” phenotype on Phyllonorycter cavella (Zeller) mines on Betula platyphylla.                Two pestiferous species have been recorded during our surveys: Micrurapteryx caraganella on ornamental Caragana arborescens in urban plantations in Amurskaya Oblast, and the lime leafminer Phyllonorycter issikii (Kumata), a species known to be native to RFE and invasive elsewhere in Russia and in European countries.                A revised checklist of RFE gracillariids has been compiled. It accounts for 135 species among which 17 species (13%) are only known to occur in RFE. The gracillariid fauna of RFE is more similar to the Japanese fauna (49%), than to the fauna of the rest of Russia (i.e European part and Siberia) (32%). 

scholarly journals A new Wagnerinus (Coleoptera: Curculionidae) from northern Japan: Description including a DNA barcode

Zootaxa ◽  
2008 ◽  
Vol 1740 (1) ◽  
pp. 15 ◽  
Author(s):  
HIRAKU YOSHITAKE ◽  
TOSHIHIDE KATO ◽  
UTSUGI JINBO ◽  
MOTOMI ITO
Keyword(s):  
Russian Far East ◽  
Far East ◽  
Dna Barcode ◽  
Plant Utilization ◽  
Sternite Viii ◽  
Biological Characters ◽  
Species Descriptions ◽  
Mitochondrial Cytochrome Oxidase ◽  
Northern Japan ◽  
The Russian Far East

Wagnerinus frugivorus sp. nov. (Ceutorhynchinae: Ceutorhynchini) is described from Hokkaido, northern Japan, based on morphological and biological characters. Morphologically, this new species closely resembles Wagnerinus carinulatus (Faust) from the Russian Far East and W. costatus (Hustache) from Japan and Korea in having an emarginate anterior margin of the rostrum, sternite VIII diminished to a pair of small sclerites, and longitudinal rows of endophallic sclerites. However, it is distinctive enough to be distinguished from W. carinulatus and W. costatus mainly by the more conspicuous mucrones of mid and hind tibiae, deeper concavities of ventrites I and II, and larger paired prominences of ventrite V. Also, W. frugivorus clearly differs from W. costatus in terms of host plant utilization, the former feeds on the seed capsules of Weigela middendorffiana (Caprifoliaceae) as larvae, while the latter utilizes midge galls on the axillary buds of Weigela species. In addition to general taxonomic information, we provide a 1366-bp fragment of the mitochondrial cytochrome oxidase subunit I gene from the holotype as a DNA barcode of W. frugivorus and discuss the importance of DNA barcoding combined with species descriptions.

scholarly journals DNA-barcoding and a new data about the karyotype of Myotis petax (Chiroptera, Vespertilionidae) in the Russian Far East

2020 ◽  
Vol 14 (4) ◽  
pp. 483-500
Author(s):  
Uliana V. Gorobeyko ◽  
Irina V. Kartavtseva ◽  
Irina N. Sheremetyeva ◽  
Denis V. Kazakov ◽  
Valentin Yu. Guskov
Keyword(s):  
Dna Barcoding ◽  
Russian Far East ◽  
Far East ◽  
Intraspecific Variability ◽  
Diagnostic Feature ◽  
Nucleotide Variability ◽  
The Family ◽  
Coi Barcoding ◽  
First Time ◽  
The Russian Far East

The DNA-barcoding and chromosomal study of the eastern water bat, Myotis petax Hollister, 1912, from the earlier unexplored localities in the Russian Far East are carried out. The COI barcoding obtained for 18 from a total of 19 individuals captured in five localities in the Russian Far East showed the low nucleotide variability with the prevalence of the central, the most abundant haplotype. The chromosomal characteristics of eight M. petax specimens (2n = 44, NFa = 52) in the Russian Far East are clarified. The number and localization of NOR in karyotype of M. petax is described at the first time and differ from distributional patterns of NOR in the sibling species M. daubentonii Kuhl, 1819 that can be used as diagnostic feature. The considerable intraspecific variability in the distribution of heterochromatin material revealed is not typical of the genus Myotis, but it has been found in other species of the family Vespertilionidae.

scholarly journals Incidence of louse flies on birds from the Russian Far East (Diptera: Hippoboscidae) and investigation of the status of some taxa by DNA barcoding

rej ◽  
2020 ◽  
Vol 29 (1) ◽  
pp. 327-335
Author(s):  
B. R. Meißner ◽  
F. Rodríguez-Vera ◽  
O. Hawlitschek ◽  
W. Heim ◽  
M. Jentzsch
Keyword(s):  
Dna Barcoding ◽  
Russian Far East ◽  
Far East ◽  
The Status ◽  
The Russian Far East

Morphological description and DNA barcoding of Hydrobaenus golovinensis sp. nov. (Diptera: Chironomidae: Orthocladiinae) from the Russian Far East

Zootaxa ◽  
2017 ◽  
Vol 4286 (2) ◽  
pp. 277
Author(s):  
EUGENYI A. MAKARCHENKO ◽  
MARINA A. MAKARCHENKO ◽  
ALEXANDER A. SEMENCHENKO ◽  
OLEG A. VELIAEV
Keyword(s):  
Dna Barcoding ◽  
Russian Far East ◽  
Far East ◽  
Instar Larva ◽  
Genetic Distances ◽  
Coi Gene ◽  
Morphological Description ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
The Russian Far East

Illustrated descriptions of adult male, pupa and fourth instar larva, as well as DNA barcoding results of Hydrobaenus golovinensis sp. nov. in comparison with closely related species H. majus Makarchenko et Makarchenko and H. sikhotealinensis Makarchenko et Makarchenko from the Russian Far East are provided. Partial mitochondrial COI gene (DNA barcoding) of the new species has been sequenced and uploaded to GenBank. Comparisons with corresponding regions of COI between H. golovinensis and other species in the genus produce K2P genetic distances of 10.3–14.3%, the values well above those associated with intraspecific variation. In contrast, genetic distances among 18 specimens are all within the range of 0–3.5%. The ML tree is also constructed using DNA barcodes obtained in the present study and those of other species of Hydrobaenus Fries from GenBank. 

New species of Ameletus Eaton, 1885 from the Russian Far East with notes on Ameletus camtschaticus Ulmer 1927 (Ephemeroptera: Ameletidae)

Zootaxa ◽  
2017 ◽  
Vol 4276 (2) ◽  
pp. 151
Author(s):  
TATIANA M. TIUNOVA ◽  
ALEKSANDR A. SEMENCHENKO ◽  
OLEG A. VELYAEV
Keyword(s):  
New Species ◽  
Developmental Stages ◽  
Mitochondrial Gene ◽  
Russian Far East ◽  
Far East ◽  
Dna Barcode ◽  
Sequence Divergence ◽  
Anal Margin ◽  
Ventral Plate ◽  
The Russian Far East

The male imagoes, larvae, and eggs of Ameletus allengaensis sp. nov. and Ameletus sirotskii sp. nov. from the Russian Far East are described. Based on the structure of the male genitalia, the imago and larvae of A. allengaensis sp. nov. and A. sirotskii sp. nov. are similar to those of A. camtschaticus, but the discovery of these new species and separation from A. camtschaticus were confirmed by studies of the morphology of the larvae and male imago, as well as molecular analysis. Identity of various developmental stages of the new species were confirmed by analysis of the mitochondrial gene cytochrome oxidase 1 (COI) DNA barcode, which was also used to reconstruct the phylogenetic relationships within the genus Ameletus. The intraspecific sequence divergence based on the Kimura-2-parameter (K2P) distance ranged from 0.0–2.5%, whereas the interspecific sequence divergence based on the K2P distance ranged from 6.2–7.9% within A. sirotskii sp. nov., A. allengaensis sp. nov. and A. camtschaticus. Male imagoes of A. allengaensis sp. nov., A. sirotskii sp. nov., and A. camtschaticus can be distinguished by the size and location of small denticles on the ventral plate of the penis. The larvae of A. allengaensis sp. nov. differ from those of A. sirotskii sp. nov. by the size of gills I and II. In A. allengaensis sp. nov., gill I is almost twice as small as gill II; in A. sirotskii sp. nov., gill I is only slightly smaller than gill II. Both new species differ from A. camtschaticus by gill II, which does not have an anal rib on the anal margin. 

Fish product mislabeling identified in the Russian far east using DNA barcoding

Gene Reports ◽  
2017 ◽  
Vol 8 ◽  
pp. 144-149 ◽  
Author(s):  
A. Nedunoori ◽  
S.V. Turanov ◽  
Yu.Ph. Kartavtsev
Keyword(s):  
Dna Barcoding ◽  
Russian Far East ◽  
Far East ◽  
Fish Product ◽  
The Russian Far East
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