IL-1β Promotes Expansion of IL-33+ Lung Epithelial Stem Cells Following RSV Infection During Infancy

2021 ◽  
Author(s):  
Luan D. Vu ◽  
Anh T.Q. Phan ◽  
Diego R. Hijano ◽  
David T Siefker ◽  
Heather Tillman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Epithelial Stem Cells ◽  
Lung Epithelial ◽  
Rsv Infection

scholarly journals Decreased expression of Met during differentiation in rat lung

2016 ◽  
Vol 60 (1) ◽  
Author(s):  
T. Kato ◽  
K. Oka ◽  
T. Nakamura ◽  
A. Ito
Keyword(s):  
Stem Cells ◽  
3D Culture ◽  
Immunofluorescence Assay ◽  
Lung Epithelial Cells ◽  
Alveolar Type ◽  
Cell Marker ◽  
Epithelial Stem Cells ◽  
Club Cell ◽  
Adult Mice ◽  
Lung Epithelial

<p>Organ-specific stem cells play key roles in maintaining the epithelial cell layers of lung. Bronchioalveolar stem cells (BASCs) are distal lung epithelial stem cells of adult mice. Alveolar type 2 (AT2) cells have important functions and serve as progenitor cells of alveolar type 1 (AT1) cells to repair the epithelium when they are injured. Hepatocyte growth factor (HGF) elicits mitogenic, morphogenic, and anti-apoptotic effects on lung epithelial cells through tyrosine phosphorylation of Met receptor, and thus is recognized as a pulmotrophic factor. To understand which cells HGF targets in lung, we identified the cells expressing Met by immunofluorescence assay. Met was strongly expressed in BASCs, which expressed an AT2 cell marker, pro-SP-C, and a club cell marker, CCSP. In alveoli, we found higher expression of Met in primary AT2 than in AT1 cells, which was confirmed using primary AT2 cells. We further examined the mitogenic activity of HGF in AT2-cell-derived alveolar-like cysts (ALCs) in 3D culture. Multicellular ALCs expressed Met, and HGF enhanced the ALC production. Taking these findings together, BASCs could also be an important target for HGF, and HGF-Met signaling could function more potent on cells that have greater multipotency in adult lung.</p>

Postnatal Lung Epithelial Stem Cells

2020 ◽  
Author(s):  
Jessica C. Orr ◽  
David R. Pearce ◽  
Robert E. Hynds
Keyword(s):  
Stem Cells ◽  
Epithelial Stem Cells ◽  
Lung Epithelial

Lung epithelial stem cells

2002 ◽  
Vol 197 (4) ◽  
pp. 527-535 ◽  
Author(s):  
W. R. Otto
Keyword(s):  
Stem Cells ◽  
Epithelial Stem Cells ◽  
Lung Epithelial

scholarly journals Lung epithelial stem cells express SARS-CoV-2 entry factors: implications for COVID-19

2020 ◽  
Author(s):  
Anna A. Valyaeva ◽  
Anastasia A. Zharikova ◽  
Artem S. Kasianov ◽  
Yegor S. Vassetzky ◽  
Eugene V. Sheval
Keyword(s):  
Stem Cells ◽  
Alveolar Epithelium ◽  
Cell Types ◽  
Regeneration Capacity ◽  
Rna Seq ◽  
Epithelial Stem Cells ◽  
Multipotent Stem Cells ◽  
High Death Rate ◽  
Lung Epithelial ◽  
High Death

AbstractSARS-CoV-2 can infiltrate the lower respiratory tract, resulting in severe respiratory failure and a high death rate. Normally, the airway and alveolar epithelium can be rapidly reconstituted by multipotent stem cells after episodes of infection. Here, we analyzed published RNA-seq datasets and demonstrated that cells of four different lung epithelial stem cell types express SARS-CoV-2 entry factors, including Ace2. Thus, stem cells can be potentially infected by SARS-CoV-2, which may lead to defects in regeneration capacity partially accounting for the severity of SARS-CoV-2 infection and its consequences.

scholarly journals Expression of SARS-CoV-2 entry factors in lung epithelial stem cells and its potential implications for COVID-19

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Anna A. Valyaeva ◽  
Anastasia A. Zharikova ◽  
Artem S. Kasianov ◽  
Yegor S. Vassetzky ◽  
Eugene V. Sheval
Keyword(s):  
Stem Cells ◽  
Alveolar Epithelium ◽  
Cell Types ◽  
Regeneration Capacity ◽  
Rna Seq ◽  
Epithelial Stem Cells ◽  
Multipotent Stem Cells ◽  
High Death Rate ◽  
Lung Epithelial ◽  
High Death

Abstract SARS-CoV-2 can infiltrate the lower respiratory tract, resulting in severe respiratory failure and a high death rate. Normally, the airway and alveolar epithelium can be rapidly reconstituted by multipotent stem cells after episodes of infection. Here, we analyzed published RNA-seq datasets and demonstrated that cells of four different lung epithelial stem cell types express SARS-CoV-2 entry factors, including Ace2. Thus, stem cells can be potentially infected by SARS-CoV-2, which may lead to defects in regeneration capacity partially accounting for the severity of SARS-CoV-2 infection and its consequences.

scholarly journals Expression and Role of Oct3/4 in Injury-Repair Process of Rat Alveolar Epithelium after 5-Fu Treatment

2017 ◽  
Vol 2017 ◽  
pp. 1-7
Author(s):  
Wen-ya Li ◽  
Xu-lv Ye ◽  
Xin-shan Jia ◽  
Lan-ling Jia
Keyword(s):  
Stem Cells ◽  
Embryonic Stem ◽  
Alveolar Epithelium ◽  
Repair Process ◽  
Lung Epithelial Cells ◽  
Clara Cell ◽  
Epithelial Stem Cells ◽  
Injury Repair ◽  
Alveolar Epithelial ◽  
Lung Epithelial

Objective. We aimed to investigate how the embryonic stem cell-related gene Oct3/4 changes during the injury-repair process of distal pulmonary epithelium induced by 5-fluorouracil (5-Fu).Methods. We have developed the lung injury model induced by 5-Fu and observed the dynamic changes of Oct3/4 by indirect immunofluorescence, Western blot, and quantitative real-time PCR. Immunofluorescence double staining was used to compare the positions of Oct3/4(+) cells and other reported alveolar epithelial stem cells.Results. Oct3/4(+) cells were not found in normal rat lung epithelial cells. However, after treatment with 5-Fu, Oct3/4(+) cells appeared at 12 h, reached the peak at 24 h, then decreased at 48 h, and eventually disappeared at 72 h. Oct3/4 was localized in the nucleus. We found that the sites of Clara cell secretory protein and surfactant protein-C dual positive cells were apparently different from Oct3/4(+) cells.Conclusions. Our results revealed that, in rat alveolar epithelium, expression of Oct3/4 could be induced after treatment with 5-Fu, then decreased gradually, and was silenced following the alveolar epithelial differentiation. We hold that Oct3/4(+) cells are lung stem cells, which can provide new evidence for identification and isolation of lung epithelial stem cells.

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