Effects of Bone Marrow Stromal Cells (BMSCs) on Behavior, Infarct Size and HIF-1α Expression in Stroke Rats

2021 ◽  
Vol 11 (11) ◽  
pp. 2274-2279
Author(s):  
Yingli Guo ◽  
Yanbin Meng ◽  
Jun Li ◽  
Hongsheng Wang ◽  
Junhong Guo
Keyword(s):  
Bone Marrow ◽  
Cognitive Function ◽  
Infarct Size ◽  
Brain Tissue ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Sham Group ◽  
Cell Number ◽  
Marrow Stromal Cells ◽  
Infarcted Tissue

This study aims to assess BMSCs’ effect on the behavior, infarct size and HIF-1α expression in stroke rats. Rats were separated into sham group, CVA group and BMSCs group with 10 rats in each group followed by analysis of neuroethology scores, brain tissue pathology and infarct size, and HIF-1α level in brain tissues. No difference of neurological scores was found between CVA group and BMSCs group after 3 hours (P > 0.05). After BMSCs transplantation, the nerve score was significantly reduced (P < 0.05) and cognitive function was significantly improved compared to CVA group. Compared with sham rats, CAV rats had a larger area of infarction and the infarcted tissue cells showed degeneration or necrosis with reduced cell number and obvious edema, which were all improved in BMSCs group. CVA group showed a larger area of infarct tissue (P < 0.05), which was reduced in BMSCs group (P < 0.05). Compared with sham group, CVA group showed significantly upregulated HIF-1α level (P < 0.05) which was reduced in BMSCs group (P < 0.05). BMSCs has a certain repair effect on the ethology of stroke rats possibly via inhibition of HIF-1α level in cerebral infarction and brain tissue.

Bone Marrow Stromal Cells Promotes Morphological Senescence of Prostate Cancer Cells and Inhibits Metastasis Associated 1 Gene Level

2022 ◽  
Vol 12 (4) ◽  
pp. 711-716
Author(s):  
Xuechuan Yan ◽  
Xin Wang ◽  
Haifeng Yang ◽  
Jian Yang ◽  
Haikun Li
Keyword(s):  
Prostate Cancer ◽  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Inhibitory Effect ◽  
Cell Number ◽  
Cell Senescence ◽  
Marrow Stromal Cells ◽  
Cancer Group ◽  
Gene Level

This study assessed the mechanism of Bone Marrow Stromal Cells (BMSCs) in prostate cancer (PC) and its effect on MTA-1 gene and PC cell senescence. PC-3 cells were assigned into QL group (prostate cancer group: normal culture) and GS group (BMSCs group: treated with BMSCs) followed by analysis of MTA-1 level, cell senescence, apoptosis and invasion. MTA-1 level in QL group (0.83±0.07) was significantly higher than GS group (0.14±0.02) (P < 0.05), indicating that BMSCs had an inhibitory effect on MTA-1 expression. Similar change of MTA-l mRNA was also found with higher level in QL group than GS group (P < 0.05). Cell senescence was found in QS group but not QL group, indicating that BMSCs promote cell senescence. Compared with GS group, QL group has a higher cell number in G0/G1 (67.13±6.45%) and S (19.59±3.35%) than GS group (G0/G1:50.51±2.19% and S: 11.42±1.61%) but lower G2/M (QL: 15.97±3.59% versus GS: 32.25±3.24%). QL group had significantly lower cell apoptosis rate at 35 h (5.21±1.2%) and 45 h (3.97±0.95%) than GS group at 35 h (17.85±1.23%), 45 h (10.21±1.26%) with elevated number of invasions. In conclusion, BMSCs promote PC-3 cell senescence and apoptosis by inhibiting the expression of MTA-1 and reduce cell invasion ability.

Glucocorticoid exposure reduces expression of sclerostin in bone marrow stromal cells

2013 ◽  
Author(s):  
Sylvia Thiele ◽  
Alexander Rauch ◽  
Jan P Tuckermann ◽  
Lorenz C Hofbauer ◽  
Martina Rauner
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells
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