Magnetic Field-Magnetic Nanoparticle Culture System Used to Grow In Vitro Murine Embryonic Stem Cells

2011 ◽  
Vol 11 (1) ◽  
pp. 36-44 ◽  
Author(s):  
Erika Regina Leal de Freitas ◽  
Paula Roberta Otaviano Soares ◽  
Rachel de Paula Santos ◽  
Regiane Lopes dos Santos ◽  
Elaine Paulucio Porfírio ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Culture System ◽  
Magnetic Nanoparticle ◽  
Embryonic Stem ◽  
Murine Embryonic Stem Cells

scholarly journals Surface antigen phenotypes of hematopoietic stem cells from embryos and murine embryonic stem cells

Blood ◽  
2009 ◽  
Vol 114 (2) ◽  
pp. 268-278 ◽  
Author(s):  
Shannon L. McKinney-Freeman ◽  
Olaia Naveiras ◽  
Frank Yates ◽  
Sabine Loewer ◽  
Marsha Philitas ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Fetal Liver ◽  
Embryonic Stem ◽  
Hematopoietic Stem ◽  
Murine Embryonic Stem Cells ◽  
Isolation And Characterization

Abstract Surface antigens on hematopoietic stem cells (HSCs) enable prospective isolation and characterization. Here, we compare the cell-surface phenotype of hematopoietic repopulating cells from murine yolk sac, aorta-gonad-mesonephros, placenta, fetal liver, and bone marrow with that of HSCs derived from the in vitro differentiation of murine embryonic stem cells (ESC-HSCs). Whereas c-Kit marks all HSC populations, CD41, CD45, CD34, and CD150 were developmentally regulated: the earliest embryonic HSCs express CD41 and CD34 and lack CD45 and CD150, whereas more mature HSCs lack CD41 and CD34 and express CD45 and CD150. ESC-HSCs express CD41 and CD150, lack CD34, and are heterogeneous for CD45. Finally, although CD48 was absent from all in vivo HSCs examined, ESC-HSCs were heterogeneous for the expression of this molecule. This unique phenotype signifies a developmentally immature population of cells with features of both primitive and mature HSC. The prospective fractionation of ESC-HSCs will facilitate studies of HSC maturation essential for normal functional engraftment in irradiated adults.

scholarly journals Gelatin Electrospun Mat as a Potential Co-culture System for In Vitro Production of Sperm Cells from Embryonic Stem Cells

2020 ◽  
Vol 6 (10) ◽  
pp. 5823-5832
Author(s):  
Mina Vardiani ◽  
Marefat Ghaffari Novin ◽  
Morteza Koruji ◽  
Hamid Nazarian ◽  
Ellen Goossens ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Culture System ◽  
Embryonic Stem ◽  
Sperm Cells ◽  
In Vitro Production ◽  
Vitro Production
Sign in / Sign up

Export Citation Format

Share Document