scholarly journals Transmission Fourier Transform Infrared Spectroscopic Imaging, Mapping, and Synchrotron Scanning Microscopy with Zinc Sulfide Hemispheres on Living Mammalian Cells at Sub-Cellular Resolution

2020 ◽  
Vol 74 (5) ◽  
pp. 544-552 ◽  
Author(s):  
Ka Lung Andrew Chan ◽  
Ali Altharawi ◽  
Pedro Fale ◽  
Cai Li Song ◽  
Sergei G. Kazarian ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Synchrotron Radiation ◽  
Zinc Sulfide ◽  
Spatial Resolution ◽  
Single Point ◽  
Spectroscopic Imaging ◽  
Fourier Transform Infrared ◽  
Living Cells ◽  
Imaging Systems ◽  
Ft Ir

Fourier transform infrared (FT-IR) spectroscopic imaging and microscopy of single living cells are established label-free technique for the study of cell biology. The constant driver to improve the spatial resolution of the technique is due to the diffraction limit given by infrared (IR) wavelength making subcellular study challenging. Recently, we have reported, with the use of a prototype zinc sulfide (ZnS) transmission cell made of two hemispheres, that the spatial resolution is improved by the factor of the refractive index of ZnS, achieving a λ/2.7 spatial resolution using the synchrotron–IR microscopy with a 36× objective with numerical aperture of 0.5. To refine and to demonstrate that the ZnS hemisphere transmission device can be translated to standard bench-top FT-IR imaging systems, we have, in this work, modified the device to achieve a more precise path length, which has improved the spectral quality of the living cells, and showed for the first time that the device can be applied to study live cells with three different bench-top FT-IR imaging systems. We applied focal plane array (FPA) imaging, linear array, and a synchrotron radiation single-point scanning method and demonstrated that in all cases, subcellular details of individual living cells can be obtained. Results have shown that imaging with the FPA detector can measure the largest area in a given time, while measurements from the scanning methods produced a smoother image. Synchrotron radiation single-point mapping produced the best quality image and has the flexibility to introduce over sampling to produce images of cells with great details, but it is time consuming in scanning mode. In summary, this work has demonstrated that the ZnS hemispheres can be applied in all three spectroscopic approaches to improve the spatial resolution without any modification to the existing microscopes.

Protein hydration in living cells probed by Fourier transform infrared (FT-IR) spectroscopic imaging

The Analyst ◽  
2017 ◽  
Vol 142 (13) ◽  
pp. 2475-2483 ◽  
Author(s):  
H. Shinzawa ◽  
B. Turner ◽  
J. Mizukado ◽  
S. G. Kazarian
Keyword(s):  
Fourier Transform ◽  
Ir Spectra ◽  
Spectroscopic Imaging ◽  
Fourier Transform Infrared ◽  
Living Cells ◽  
Protein Hydration ◽  
Ft Ir ◽  
Ir Spectroscopic

FT-IR spectra of a HEK cell were analyzed with 2D disrelation mapping to reveal molecular states of water and protein hydration.

Time-Resolved Fourier Transform Infrared Spectroscopic Imaging

2003 ◽  
Vol 57 (4) ◽  
pp. 357-366 ◽  
Author(s):  
Rohit Bhargava ◽  
Ira W. Levin
Keyword(s):  
Fourier Transform ◽  
Imaging Modality ◽  
Spectroscopic Imaging ◽  
Fourier Transform Infrared ◽  
Dynamic Processes ◽  
Time Resolved ◽  
Ir Imaging ◽  
Infrared Spectroscopic ◽  
Infrared Spectroscopic Imaging ◽  
Ft Ir

Fourier transform infrared (FT-IR) imaging allows simultaneous spectral characterization of large spatial areas due to its multichannel detection advantage. The acquisition of large amounts of data in the multichannel configuration results, however, in a poor temporal resolution of sequentially acquired data sets, which limits the examination of dynamic processes to processes that have characteristic time scales of the order of minutes. Here, we introduce the concept and instrumental details of a time-resolved infrared spectroscopic imaging modality that permits the examination of repetitive dynamic processes whose half-lives are of the order of milliseconds. As an illustration of this implementation of step-scan FT-IR imaging, we examine the molecular responses to external electric-field perturbations of a microscopically heterogeneous polymer–liquid crystal composite. Analysis of the spectroscopic data using conventional univariate and generalized two-dimensional (2D) correlation methods emphasizes an additional capability for accessing of simultaneous spatial and temporal chemical measurements of molecular dynamic processes.

Variation in Composition of Bone Surrounding Implants

2006 ◽  
Vol 309-311 ◽  
pp. 19-22 ◽  
Author(s):  
Taketoshi Suwa ◽  
Toshiro Sakae ◽  
Hiroshi Nakada ◽  
Racquel Z. LeGeros ◽  
Kihei Kobayashi
Keyword(s):  
Fourier Transform ◽  
Light Microscopy ◽  
Crystal Size ◽  
Polarized Light ◽  
Spectroscopic Imaging ◽  
Fourier Transform Infrared ◽  
Polarized Light Microscopy ◽  
Tissue Structure ◽  
Infrared Spectroscopic Imaging ◽  
Ft Ir

Our studies previously demonstrated that new bone formed around implants can be classified into 3 or 4 types based on tissue structure and composition. Results of the present study, using polarized light microscopy, and microscopic Fourier transform infrared spectroscopic imaging (micro-FT-IR) and micro-XRD to examine different areas in the peri-implant new bone, suggest differences in crystallinity (crystal size) between pre-existing bone and peri-implant new bone.

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