scholarly journals Blood Sampling Technique for Lactate and Pyruvate Estimation in Children

1977 ◽  
Vol 14 (1-6) ◽  
pp. 285-287 ◽  
Author(s):  
L. A. A. Kollée ◽  
J. L. Willems ◽  
A. F. M. De Kort ◽  
L. A. H. Monnens ◽  
J. M. F. Trijbels
Keyword(s):  
Blood Sample ◽  
Sampling Technique ◽  
Blood Sampling ◽  
Intravenous Cannula ◽  
Lactate And Pyruvate ◽  
Effects Of Stress

The effects of stress and muscular movements caused by direct venepuncture and of venestasis on the lactate and pyruvate levels in children have been examined. Direct venepuncture and venestasis significantly influence the obtained values. To measure reliable values for pyruvate and lactate in blood of children it seems advisable to insert an indwelling intravenous cannula. After 45 min of rest in bed the blood sample can be obtained.

scholarly journals A Study on Hematopoietic Stem Cell Donation Volunteer Retention between Swab Sampling Approach and Blood Sampling Approach: Evidence from Shanghai, China

2021 ◽  
Vol 18 (8) ◽  
pp. 4027
Author(s):  
Ke Yan ◽  
Gang Zhang ◽  
Guoqiang Zhao ◽  
Baosong Liu ◽  
Jun Lu
Keyword(s):  
Stem Cell ◽  
Blood Sample ◽  
Hematopoietic Stem Cell ◽  
Blood Sampling ◽  
Distribution Characteristics ◽  
Hematopoietic Stem ◽  
Volunteer Retention ◽  
Sample Group ◽  
Stem Cell Donation ◽  
Sampling Approach

The loss of hematopoietic stem cell donation (HSCD) volunteers is widespread worldwide. This study analyzed the distribution characteristics of volunteer retention between the swab sampling approach and blood sampling approach. The Shanghai branch of the China Bone Marrow Donation Program conducted a telephone follow-up with 18,963 volunteers to understand volunteer retention. Multiple logistic regression was used to analyze the distribution characteristics of volunteer retention between two different sampling approaches, and a forest plot was used to observe the distribution trend. Only 32.37% of the volunteers could be contacted, and the loss of volunteers was severe. The volunteer retention is influenced by sampling approaches and demographic characteristics, and Shanghai natives, the highly educated, and students had better retention. The volunteer retention of the swab group was better among young people and technicians, while the volunteer retention of the blood sample group was lower among public officials and workers, and the volunteer retention in the blood sample group was more significantly affected by changes in population characteristics. To enhance the stability of volunteers, managers should improve the contact channels and frequency, expand the ratio of stable volunteers, strengthen volunteer education in the process of collecting blood samples, and respect individuals’ willingness.

Effect of method of blood sample collection on adrenal activity in farmed red deer and sheep following administration of ACTH

1998 ◽  
Vol 67 (1) ◽  
pp. 157-164 ◽  
Author(s):  
I. Ferre ◽  
P. J. Goddard ◽  
A. J. Macdonald ◽  
C. A. Littlewood ◽  
E. I. Duff
Keyword(s):  
Blood Sample ◽  
Plasma Cortisol ◽  
Sampling Method ◽  
Red Deer ◽  
Blood Sampling ◽  
Domestic Sheep ◽  
Sample Collection ◽  
Acth Stimulation ◽  
Treatment Groups ◽  
Stimulation Tests

AbstractThe effect of method of blood sample collection (automatic blood sampling equipment (ABSE) v. manual) on cortisol and progesterone concentrations was investigated in 20 farmed red deer hinds and 20 domestic sheep ewes following dexamethasone and exogenous ACTH administration. Ten animals were subjected to either automatic sampling or manual sampling via jugular venipuncture in 1 week, with the treatment groups reversed in the 2nd week. The ABSE was programmed to collect a blood sample, then deliver 2 mg dexamethasone, collect a further blood sample 120 min later and then inject 100 fig ACTH. Thereafter, samples were collected at 15-min intervals during a 2·5 h period (12 samples in total). In the manual injection and sampling treatment, four samples were collected: (1) before dexamethasone administration, (2) before ACTH administration, (3) 60 min after ACTH administration, and (4) 150 min after ACTH administration. The success rate of blood sampling with ABSE was 80%. The overall mean packed cell volume (PCV) from samples collected by ABSE from both hinds and ewes was significantly lower than that from samples collected manually (P < 0·01) and PCV declined with time in manually sampled animals (P < 0·01). Plasma cortisol concentrations peaked at 45 min after ACTH administration in sheep and deer. In sheep, there was a marked fluctuation in the plasma cortisol concentrations with time. Both deer and sheep showed a reduced cortisol response to ACTH during week 2 irrespective of sampling method suggesting down-regulation of the response to ACTH. Maximum mean plasma progesterone concentration was reached at 15 to 30 min after ACTH administration. No significant differences in cortisol and progesterone responses due to blood sampling method were found in animals receiving prior dexamethasone treatment. This demonstrates that the ABSE has the ability to be used to effectively conduct ACTH stimulation tests without the need to handle the animals during the test.

Effects of open field testing and associated handling v. handling alone on the adrenocortical reactivity of piglets around weaning

2002 ◽  
Vol 74 (3) ◽  
pp. 485-491 ◽  
Author(s):  
M. S. Herskin ◽  
K. H. Jensen
Keyword(s):  
Blood Sample ◽  
Open Field ◽  
Field Testing ◽  
Blood Sampling ◽  
Cortisol Response ◽  
Temporal Development ◽  
Novel Object ◽  
Before And After ◽  
Pre Treatment

AbstractEffects of open field testing and associated handling (including blood sampling) v. handling (with blood sampling) alone on adrenocortical reactivity were investigated in piglets around weaning. After weaning at day 28, piglets were mixed to form eight replicates with three litter each and kept on slatted floors. Treatments were open field/novel object testing and associated handling (OFT + H) v. handling alone (H) (no. = 40). Testing took place on the following days (weaning day 0): -4, 0, 1, 4 and 8 (no. = 16). The open field/novel object test lasted 10 min and blood was sampled by venipuncture before and after the test and/or handling procedures. In the first blood sample, plasma concentration of cortisol did not differ between OFT + H and H piglets. In the second blood sample, however, OFT + H piglets had a higher concentration of cortisol and a larger increase in cortisol than H piglets (P < 0·01). In the first blood sample the concentration of cortisol was affected by day (P < 0·05), with concentration on day -4 being lower than those on day 0, 1, and 4 and the concentration on day 8 was lower than the concentration on day 0 as well. However, no differences were found between days for the second blood sample nor the adrenocortical reactivity. In conclusion, the increase in pre-treatment cortisol in the first 4 days after weaning as well as the lack of changes in the adrenocortical reactivity after weaning suggest that the temporal development of HPA activity and reactivity in piglets after weaning are comparable with other, more standardized long-term stressors. Exposure to an open field/novel object test and associated handling results in higher adrenocortical reactivity than handling alone but the latter (including blood sampling) in itself accounts for proportionately 0·74 of the cortisol response.

Validation of a minimally invasive blood-sampling technique for the analysis of hormones in domestic rabbits, Oryctolagus cuniculus (Lagomorpha)

2004 ◽  
Vol 135 (1) ◽  
pp. 100-107 ◽  
Author(s):  
Christian C Voigt ◽  
Mirja Faßbender ◽  
Martin Dehnhard ◽  
Gudrun Wibbelt ◽  
Katarina Jewgenow ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Oryctolagus Cuniculus ◽  
Sampling Technique ◽  
Blood Sampling ◽  
Domestic Rabbits

Microdialysis combined blood sampling technique for the determination of rosiglitazone and glucose in brain and blood of gerbils subjected to cerebral ischemia

2011 ◽  
Vol 54 (4) ◽  
pp. 759-764 ◽  
Author(s):  
Wayne H.-H. Sheu ◽  
Hsiu-Chun Chuang ◽  
Shiu-Min Cheng ◽  
Maw-Rong Lee ◽  
Chi-Chi Chou ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Sampling Technique ◽  
Blood Sampling

ASSESSMENT OF A LANCET-AND-SWAB BLOOD SAMPLING TECHNIQUE FOR SURVEILLANCE OF ELEPHANT ENDOTHELIOTROPIC HERPESVIRUS INFECTION

2017 ◽  
Vol 48 (3) ◽  
pp. 659-667 ◽  
Author(s):  
Javier Lopez ◽  
L.do. Vet., M.Sc. ◽  
Jonathan Haycock ◽  
Andrew Mckenzie ◽  
Katharina Seilern-Moy ◽  
...  
Keyword(s):  
Sampling Technique ◽  
Blood Sampling ◽  
Herpesvirus Infection

scholarly journals Nurses’ Experiences of Blood Sample Collection from Children: A Qualitative Study from Swedish Paediatric Hospital Care

2021 ◽  
Author(s):  
Henrik Hjelmgren ◽  
Britt-Marie Ygge ◽  
Björn Nordlund ◽  
Nina Andersson
Keyword(s):  
Focus Group ◽  
Qualitative Study ◽  
Blood Sample ◽  
Hospital Care ◽  
Blood Sampling ◽  
Sample Collection ◽  
Focus Group Interviews ◽  
Paediatric Hospital ◽  
Group Interviews ◽  
Analytical Errors

Abstract Background: Blood sampling collections are necessary and important for diagnosis and treatment in paediatric hospital care. Nurses play an active role in helping the children with the blood sampling experience. Unfortunately, the blood sampling collection procedure is often affected by pre-analytical errors, leading to consequences such as delayed diagnosis, treatment and hospital stay, as well as repeated sampling. Moreover, children state that needle procedures are the worst experience of their hospital stay. Nurses working in children’s hospitals are responsible for conducting most of the needle related procedures but their experience of errors occurring during blood sample collection is unknown. The aim of this study therefore was to describe paediatric nurses’ experiences of blood sampling collections from children. Method: We used a qualitative study design with a (reflexive) thematic analysis (TA) method. Three focus group interviews were conducted, with 19 nurses from Sweden working at two different paediatric hospitals, focusing on their experiences of the blood sample collection procedure. Results: From the three focus group interviews we analysed patterns and meanings of the following themes: Paediatric blood sampling is a challenge for the nurses, Nurses’ feelings of frustration with unsuccessful samplings, Nurses believe in team work, Venous blood sampling was experienced as the best option, and Nurses’ thoughts and needs regarding skills development in paediatric blood sampling. Conclusion: The narrative results of this study illustrate that nurses working in paediatric hospital care face a big challenge in blood sampling collection from children. The nurses felt frustrated due to unsuccessful blood samplings and frequently could not understand why pre-analytical errors occurred. Nevertheless, they felt strengthened by colleagues in their team and shared feelings of responsibility to help each other with this complex procedure. The implications of this study are that paediatric hospital care needs to focus on improving guidelines for and increasing competence in blood sampling children and helping nurses to understand why samplings may be unsuccessful and how this can be avoided.

scholarly journals RESPON NYERI PADA NEONATUS PASKA PENGAMBILAN DARAH SETELAH DIBEDONG DI RUANG NEONATUS RSUD NGUDI WALUYO WLINGI

2015 ◽  
Vol 2 (1) ◽  
pp. 050-054
Author(s):  
Tri Winarti
Keyword(s):  
Muscle Tone ◽  
Venous Blood ◽  
Behavioral Responses ◽  
Sampling Technique ◽  
Sampling Procedure ◽  
Blood Sampling ◽  
Invasive Procedures ◽  
Facial Muscles ◽  
Sampling Procedures ◽  
Venous Blood Sampling

Newborns in hospitals often have a variety of invasive procedures, so that the baby is experiencingpain. Baby’s sense of discomfort and fear which will result from the pain can be observed throughthe tears. While wrapping the newborn to fit in a blanket (swaddling) can soothe a fussy baby or cry. Thepurpose of this study was to identify the effect of swaddling on pain response in neonates after venousblood sampling procedures in hospitals Ngudi Waluyo Wlingi. This study was descriptive. Samplestaken by accidental sampling technique with a sample of 35 neonates. The results showed that swaddledneonates after venous blood sampling procedure was 80% (28 respondents) pulse between 120–160times per minute, 100% (35 respondents) respiration between 30–60 times per minute and 51.4% (18respondents) SaO2 is greater than 92%. Behavioral responses such as shrill and loud cry was 8.4% (24respondents), stretching in some facial muscles and facial muscles stretching along each was 4.2% (12respondents), a strong move is 5.95% (17 respondents ), and increased muscle tone face with flexion ofthe fingers and toes was 7.7% (22 respondents).Use of swaddling post venous blood sampling proceduresin neonates can be used as a way of making neonates feel comfortable and calm.

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