scholarly journals Improved Measurement of Monoclonal Paraproteins in Serum Using Agarose Gel Electrophoresis

1987 ◽  
Vol 24 (1) ◽  
pp. 73-76 ◽  
Author(s):  
P M Dennis ◽  
B Biegler ◽  
R Papas
Keyword(s):  
Cellulose Acetate ◽  
Gel Electrophoresis ◽  
Brilliant Blue ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Brilliant Blue G ◽  
Cellulose Acetate Electrophoresis ◽  
Coomassie Brilliant Blue ◽  
Ponceau S ◽  
Coomassie Brilliant

Serum paraproteins can be accurately and precisely measured by means of agarose gel electrophoresis and densitometry at 520 nm after staining with Coomassie Brilliant Blue G. The results obtained for this method agree closely with the quantity of paraprotein recoverable from preparative agarose gel electrophoresis. The method is preferable to cellulose acetate electrophoresis stained with Ponceau S.

scholarly journals Elution of proteins from polyacrylamide eels: a simple and economic procedure

1989 ◽  
Vol 31 (1) ◽  
pp. 44-47
Author(s):  
Vera Bongertz
Keyword(s):  
Gel Electrophoresis ◽  
Brilliant Blue ◽  
Polyacrylamide Gels ◽  
Coomassie Brilliant Blue ◽  
Coomassie Brilliant

A simplified methodology for the quantitative electroelution of proteins from polyacrylamide gels is described. After staining with Coomassie Brilliant Blue R 250, the identified bands are excised from the gel and the proteins eluted using a procedure developed for use in conventional tube gel electrophoresis equipment.

MEASUREMENT OF CANINE PITUITARY PROLACTIN

1973 ◽  
Vol 56 (2) ◽  
pp. 245-NP ◽  
Author(s):  
P. G. SALUJA ◽  
M. GRONOW ◽  
J. M. HAMILTON
Keyword(s):  
Isoelectric Focusing ◽  
Isoelectric Point ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Brilliant Blue ◽  
Coomassie Brilliant Blue ◽  
Ovine Prolactin ◽  
Pituitary Prolactin ◽  
Coomassie Brilliant

SUMMARY Isoelectric focusing in polyacrylamide gel followed by staining with Coomassie brilliant blue was used for the densitometric estimation of ovine prolactin standard and canine pituitary prolactin. The results were compared with those obtained by conventional polyacrylamide gel electrophoretic procedures and isoelectric focusing was found to be a valid technique for the estimation of prolactin and to offer greater technical convenience. The mobility of ovine and canine prolactin was similar in isoelectric focusing and gel electrophoresis. The isoelectric point of ovine and canine prolactin was found to be 6·17 and 6·61 respectively. Constant levels of prolactin were found in the pituitaries of bitches at oestrus.

Automatic assay of urinary protein using Coomassie Brilliant Blue G-250

1981 ◽  
Vol 113 (1) ◽  
pp. 197-201 ◽  
Author(s):  
Kiyoko Sano ◽  
Kiyoko Kanamori ◽  
Akihiko Shiba ◽  
Makoto Nakao
Keyword(s):  
Urinary Protein ◽  
Brilliant Blue ◽  
Brilliant Blue G ◽  
Coomassie Brilliant Blue ◽  
Coomassie Brilliant
Sign in / Sign up

Export Citation Format

Share Document