Direct Tissue PCR and Genotyping for Species Identification in a Case of Laryngeal Blastomycosis
Keyword(s):
Otolaryngologic manifestations of infection with Blastomyces species are extremely rare and restricted geographically to recognized endemic regions. Here, we describe a case of laryngeal blastomycosis that presented as slowly progressive dysphonia. While a preliminary diagnosis was made using routine histopathology, a species identification of Blastomyces dermatitidis was made using polymerase chain reaction amplification and rapid genotyping without the need for fungal culture. All symptoms resolved following 1 month of antifungal therapy. Rapid molecular differentiation of B dermatitidis from Blastomyces gilchristii provides important insights into pathogenesis given recent recognition of differences in clinical spectra.
2003 ◽
Vol 48
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pp. 857-865
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1996 ◽
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pp. 471-478
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pp. 371-374
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pp. 117-131
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1990 ◽
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