Leukotrienes C4 and D4 Increase the Ciliary Beat Frequency in Human Upper Airway Mucosa in Vitro

1998 ◽  
Vol 118 (4) ◽  
pp. 472-477 ◽  
Author(s):  
Carlos B. Cyrus ◽  
Bin Yang ◽  
Thomas V. McCaffrey
Keyword(s):  
Beat Frequency ◽  
Upper Airway ◽  
Ciliary Beat Frequency ◽  
Airway Mucosa ◽  
Contrast Microscopy ◽  
Leukotriene Receptor ◽  
Upper Respiratory ◽  
In Vitro Effects ◽  
Ciliary Beat

It has been suggested that leukotrienes C4 (LTC4) and D4 (LTD4) released from upper respiratory mucosa influence mucociliary transport during allergic reactions. We studied the in vitro effects of leukotrienes C4 and D4 on the ciliary beat frequency (CBF) of human adenoid explants over a 5-hour period. Tissue explants were cultured at 35° C in Minimum Essential Medium Eagle (MEM). The CBF was measured using phase contrast microscopy and microphotometry. Measurements of CBF were recorded in medium alone and in medium containing LTC4 or LTD4 at concentrations of 10−8 and 10−6 M. LTC4 and LTD4 increased CBF at concentrations of 10−8 and 10−6 M with increases of 20.51% ± 2.69% and 29.84% ± 4.06%, respectively. To determine the specificity of the LTC4 and LTD4 effects, the ciliated epithelium was treated with the specific leukotriene receptor antagonist LY-171,883 before administration of LTC4 and LTD4. LY-171,883 (10−6 M) significantly inhibited the ciliostimulatory effects of both leukotrienes. Our findings indicate that LTC4 and LTD4 increase CBF in vitro by activation of the LTD4 receptor.

Poster 22: Leukotrienes C4 and D4 Increase the Ciliary Beat Frequency in Human Upper Airway Mucosa in Vitro

1996 ◽  
Vol 115 (2) ◽  
pp. P130-P130
Author(s):  
Carlos B. Cyrus ◽  
Thomas V. McCaffrey ◽  
Bin Julia Yang
Keyword(s):  
Beat Frequency ◽  
Upper Airway ◽  
Ciliary Beat Frequency ◽  
Airway Mucosa ◽  
Ciliary Beat

In Vitro Study on the Influence of N-Chlorotaurine on the Ciliary Beat Frequency of Nasal Mucosa

2003 ◽  
Vol 17 (3) ◽  
pp. 149-152 ◽  
Author(s):  
Edmund Hofer ◽  
Andreas Neher ◽  
Andreas R. Gunkel ◽  
Markus Nagl
Keyword(s):  
Nasal Mucosa ◽  
Beat Frequency ◽  
In Vitro Study ◽  
Ciliary Beat Frequency ◽  
Clinical Investigations ◽  
Photometric Technique ◽  
In Vitro Effects ◽  
Toxic Potential ◽  
Ciliary Beat

Background The aim of this study was to investigate the in vitro effects of N-chlorotaurine (NCT), a new endogenous antimicrobial agent, on the nasal ciliary beat frequency (CBF) in nasal mucosa samples of 10 adult patients who underwent conchotomy. Methods CBF was measured by a photometric technique, combining a light microscope, a photometer, a photograph multiplier, and a computed analyzing unit. Results CBF decreased ∼10% after 20 minutes of incubation in aqueous 1% NCT solution compared with 3% in 0.9% saline, and 0.1 and 0.01% NCT had no effect. However, a solution of 7% cocaine, which is used routinely for shrinking the nasal mucosa before rhinoscopy lowered the CBF to 50% after the same incubation time. Conclusion These results confirm the very low toxic potential of NCT, and they are fundamental for clinical investigations regarding its topical application in the nasal and paranasal sinuses.

Modulation of Ciliary Beat Frequency in the Upper Airway by Prostaglandins

1996 ◽  
Vol 10 (4) ◽  
pp. 229-234 ◽  
Author(s):  
Rodney J. Schlosser ◽  
Ramon A. Franco ◽  
Judith M. Czaja ◽  
Thomas V. McCaffrey
Keyword(s):  
Culture Media ◽  
Beat Frequency ◽  
Upper Airway ◽  
Ciliary Beat Frequency ◽  
Prostaglandin E ◽  
Ciliary Activity ◽  
Maximum Increase ◽  
Endogenous Prostaglandins ◽  
Ciliary Beat

Prostaglandins are ubiquitous within tissues and participate in several local regulatory functions. Previous reports have presented conflicting evidence on their role in ciliary activity in the upper airway. We studied the in vitro effects of prostaglandin E2 (PGE2) and a prostaglandin I2 analog (Iloprost) on the ciliary beat frequency (CBF) of human adenoid explants and rabbit sinus mucosa. Tissue explants were maintained in culture at 35°C. PGE2 or Iloprost was added to the culture media at concentrations of 10–6, to 10–12 M. CBF was determined using phase contrast microscopy and microphotometry. PGE2 increased CBF with a maximum increase of 54.6% in rabbit mucosa (P < 0.01) and 20.0% in human adenoid (P < 0.009). Iloprost increased CBF with a maximum increase of 35.2% in rabbit mucosa (P < 0.02) and 16.4% in human adenoid (P < 0.03). Induced production of endogenous prostaglandins was determined by treating specimens with a cyclooxygenase inhibitor (diclofenac) prior to addition of PGE2 or Iloprost. Diclofenac had no effect on the ciliostimulatory effects of PGE2 (P > 0.36), however it significantly decreased the ciliostimulatory effect of Iloprost (P < 0.037). Both PGE2 and Iloprost stimulate ciliary motility, yet the action of PGE2 is direct, whereas Iloprost stimulates cilia by release of endogenous prostaglandins in both the human and rabbit.

Histamine and leukotriene C4 effects on in vitro ciliary beat frequency of human upper respiratory cilia

1994 ◽  
Vol 251 (6) ◽  
pp. 325-328 ◽  
Author(s):  
P. J. Schuil ◽  
J. M. E. van Gelder ◽  
M. ten Berge ◽  
K. Graamans ◽  
E. H. Huizing
Keyword(s):  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Leukotriene C4 ◽  
Respiratory Cilia ◽  
Upper Respiratory ◽  
Ciliary Beat

Substance P and Ciliary Beat of Human Upper Respiratory Cilia in Vitro

1995 ◽  
Vol 104 (10) ◽  
pp. 798-802 ◽  
Author(s):  
Paul J. Schuil ◽  
Maartje Ten Berge ◽  
Kees Graamans ◽  
José M. E. Van Gelder ◽  
Egbert H. Huizing
Keyword(s):  
Substance P ◽  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Computer Assisted ◽  
Ciliary Activity ◽  
Upper Respiratory Tract ◽  
Upper Respiratory ◽  
Ciliary Beat

On stimulation of trigeminal nerve endings, neuropeptides are released into the nasal mucosa. Among these neuropeptides is substance P (SP). In this study, we determined the effect in vitro of SP, as well as SP together with thiorphan, a blocker of the SP-degrading enzyme neutral endopeptidase, on the ciliary beat frequency (CBF) of the human upper respiratory tract. Ciliated epithelium of human adenoid tissue was used in the experiments. The CBF was measured by means of a computer-assisted photoelectric method. Substance P (10−8 to 10−5 mol/L, n = 7) showed a small but statistically significant dose-dependent decrease in CBF. On perfusion with SP (10−8 to 10−5 mol/L, n = 8) in combination with thiorphan, no statistically significant effect was found. We conclude that SP does not have a direct effect on ciliary activity to such an extent that it will affect mucociliary transport in vivo.

Effects of Prostaglandins D, and E2on Ciliary Beat Frequency of Human Upper Respiratory Ciliain Vitro

1995 ◽  
Vol 115 (3) ◽  
pp. 438-442 ◽  
Author(s):  
P. J. Schuil ◽  
M. Ten Berge ◽  
J. M. E. Van Gelder ◽  
K. Graamans ◽  
E. H. Huizing
Keyword(s):  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Upper Respiratory ◽  
Ciliary Beat

The effects of in vitro cotitine on nasal ciliary beat frequency

1995 ◽  
Vol 20 (5) ◽  
pp. 465-469 ◽  
Author(s):  
A. M. AGIUS ◽  
M. WAKE ◽  
A. L. PAHOR ◽  
A. SMALLMAN
Keyword(s):  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Ciliary Beat

Effects of ethanol on in vitro ciliary motility

1988 ◽  
Vol 65 (4) ◽  
pp. 1617-1620 ◽  
Author(s):  
D. R. Maurer ◽  
J. Liebman
Keyword(s):  
Beat Frequency ◽  
Slow Motion ◽  
Ciliary Beat Frequency ◽  
Blood Levels ◽  
Ciliary Beating ◽  
Ciliary Motility ◽  
Social Drinking ◽  
High Concentrations ◽  
Ciliary Beat

Consumption of ethanol can impair lung function and slow total lung clearance. High concentrations of ethanol have been shown to slow or arrest ciliary beating. This study examined the effects of concentrations of alcohol comparable to blood levels achieved from social drinking on ciliary beat frequency. We obtained ciliated cells by brushing the trachea of unanesthetized sheep during fiber-optic bronchoscopy. The cells were suspended in a perfusion chamber and physiological conditions were maintained in vitro. Ciliary beat frequency and synchrony were determined by slow-motion analysis of video images obtained by interference contrast microscopy. Metachronal ciliary coordination was observed in all preparations. The ciliary beat frequency was stimulated at ethanol concentrations from 0.01 up to but not including 0.1%, unchanged at 0.5 and 1%, and slowed at 2%. While confirming inhibition of ciliary motility at very high ethanol levels, we observed no acute impairment of ciliary function at ethanol concentrations comparable to those achieved from social drinking. Indeed, we found an unexpected stimulation of ciliary beating at low levels of ethanol. How this alteration in ciliary beating would affect pulmonary clearance remains unknown at this time.

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