Stability of extemporaneously prepared preservative-free methylphenidate 5 mg/mL intravenous solution

Disclaimer In an effort to expedite the publication of articles , AJHP is posting manuscripts online as soon as possible after acceptance. Accepted manuscripts have been peer-reviewed and copyedited, but are posted online before technical formatting and author proofing. These manuscripts are not the final version of record and will be replaced with the final article (formatted per AJHP style and proofed by the authors) at a later time. Purpose To advance the implementation of consciousness-promoting therapies in patients with acute disorders of consciousness, the availability of potential therapeutic agents in formulations suitable for administration in hospitalized patients in the presence of complex comorbid conditions is paramount. The purpose of this study is to evaluate the long-term stability of extemporaneously prepared preservative-free methylphenidate hydrochloride (HCl) 5 mg/mL intravenous solution for experimental use. Methods A methylphenidate 5 mg/mL solution was prepared under proper aseptic techniques with Methylphenidate Hydrochloride, USP, powder mixed in sterile water for solution. Methylphenidate HCl 5 mg/mL solution was sterilized by filtration technique under USP <797>–compliant conditions. Samples were stored refrigerated (2-8°C) and analyzed at approximately days 1, 30, 60, 90, 180, and 365. At each time point, chemical and physical stability were evaluated by visual inspection, pH measurement, membrane filtration procedure, turbidometric or photometric technique, and high-performance liquid chromatography analysis. Results Over the 1-year study period, the samples retained 96.76% to 102.04% of the initial methylphenidate concentration. There was no significant change in the visual appearance, pH level, or particulate matter during the study period. The sterility of samples was maintained and endotoxin levels were undetectable throughout the 1-year stability period. Conclusion Extemporaneously prepared preservative-free methylphenidate 5 mg/mL intravenous solution was physically and chemically stable at 32, 61, 95, 186, and 365 days when stored in amber glass vials at refrigerated temperatures (2-8°C).

Analysis of physical properties of the HAT-P-12b planetary system

The exoplanet HAT-P-12b was observed at the Sierra Remote Observatories by a 0.7 meters diameter reflective robotic telescope CDK700 with the CCD photometric technique. This research using 317 images of the HAT-P-12b on April 14, 2017. They were analyzed the photometric photometry and calculated the physical properties by the AstroImageJ program. The results show that the radius of the exoplanet HAT-P-12b is 0.951 RJ, orbital period P about 3.2105 days. The center of page traverse Tc is 2457857.166110000 (BJD) and the total time of traverse T is 0.093519 days. The Star and Exoplanet View program was created the HAT-P-12b model that the decreasing brightness with 1.94864 percent the average distance between the exoplanet HAT-P-12b and the center is 0.0388 AU.

Development of a spectrophotometric method for determination of ions Cu (II) C N- (2-hydroxybenzoyl) -N '- (p-tosil)hydrazine in ammonia media

A spectrophotometric method for the determination of Cu (II) ions with N- (2-hydroxybenzoyl) -N '- (p-tosyl) hydrazine (GBSH) in ammonia media has been developed. The optimal conditions for the formation of a complex compound are found: the wavelength of maximum light absorption; pH complexation; optimal time for color development; the amount of photometric reagent. Under optimal conditions of complexation of GBSG with Cu (Ⅱ) ions (λ = 401 nm, pH = 9.2, τ = 30 minutes, VGBSG = 3.75 ml), a calibration graph is constructed. The Bouguer-Lambert-Beer law is fulfilled in the range from 0.13 to 1.59 mg Cu (II) / 25 ml. The true molar light absorption coefficient of the complex compound of HBSG with Cu (Ⅱ) ions in the ammonia medium was 1480. The molar ratios in the solution of the complex compound were determined by the methods of saturation, shift of equilibria and conductometric titration - [Cu (Ⅱ)]: [HBSG] = 1: 1. According to Babko's method, the conditional constant of stability of the GBSG complex with Cu (Ⅱ) ions was calculated and it was 4.4 × 1010. The reproducibility and relative error of the developed photometric technique were determined by the method of mathematical statistics.

Stability of Extemporaneously Prepared Preservative-Free Methylphenidate 5 mg/mL Intravenous Solution

Purpose: To advance the implementation of consciousness-promoting therapies in patients with acute disorders of consciousness, the availability of potential therapeutic agents in formulations suitable for administration in hospitalized patients in the presence of complex comorbid conditions is paramount. The purpose of this study is to evaluate the long-term stability of extemporaneously prepared preservative-free methylphenidate 5 mg/mL intravenous solution. Methods: A methylphenidate 5 mg/mL solution was prepared under proper aseptic techniques with Methylphenidate HCl USP powder mixed in sterile water for solution. Methylphenidate HCl 5 mg/ml solution was sterilized by filtration technique under USP <797> compliant conditions. Samples were stored refrigerated (2-8C) and analyzed at approximately day 1, 30, 60, 90, 180, and 365. At each time point, chemical and physical stability was evaluated by visual inspection, pH measurement, membrane filtration procedure, turbidometric or photometric technique, and high-performance liquid chromatography (HPLC) analysis. Results: Over the 1 year study period, the sample retained 96.76-102.04% of initial methylphenidate concentration. There was no significant change in the visual appearance, pH level or particulate matter during the study period. The sample maintained bacterial and fungi sterility, and endotoxin levels were undetectable throughout the 1 year stability period. Conclusions: Extemporaneously prepared preservative-free methylphenidate 5 mg/mL intravenous solution was physically and chemically stable for up to 365 days when stored in amber glass vials at refrigerated temperatures (2-8C).

Surface Functionalization by Hydrophobin-EPSPS Fusion Protein Allows for the Fast and Simple Detection of Glyphosate

Glyphosate, the most widely used pesticide worldwide, is under debate due to its potentially cancerogenic effects and harmful influence on biodiversity and environment. Therefore, the detection of glyphosate in water, food or environmental probes is of high interest. Currently detection of glyphosate usually requires specialized, costly instruments, is labor intensive and time consuming. Here we present a fast and simple method to detect glyphosate in the nanomolar range based on the surface immobilization of glyphosate’s target enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) via fusion to the hydrophobin Ccg2 and determination of enzyme activity with a malachite green assay, which is a common photometric technique to measure inorganic phosphate (Pi). The assay demonstrates a new approach for a fast and simple detection of pesticides.

Bok globule CB 17: polarization, extinction and distance

In this article, the results obtained from a polarimetric study of Bok globule CB 17 in both optical and submillimetre wavelengths are presented. Optical polarimetric observations in the R band (λ = 630 nm, Δλ = 120 nm) were conducted with the 1.04-m Sampurnanand Telescope, Aryabhatta Research Institute of observational sciencES (ARIES), in Nainital, India on 2016 March 9, while submillimetre polarimetric data are taken from the Submillimetre Common-User bolometer array POLarimeter (SCUPOL) data archive, which has been reanalysed. The contours of Herschel1 Spectral and Photometric Imaging Receiver (SPIRE) 500-μm dust continuum emission of CB 17 (typically a cometary-shaped globule) are overlaid on the Digital Sky Survey (DSS) image of CB 17 along with polarization vectors (optical and submm). The magnetic field strength at the core of the globule is estimated to be 99 μG. Using near-infrared photometric technique and Gaia data, the distance to CB 17 is found to be 253 ± 43 pc. The correlation between the various quantities of the globule is also studied. It is observed that the magnetic field in the cloud core as revealed by polarization measurements of the submillimetre dust emission is found to be almost aligned along the minor axis of the globule, which fits the magnetically regulated star formation model. A misalignment between core-scale magnetic field direction and molecular outflow direction is also found.

Triglyceride Levels on Electronic Cigarette Vapers in Palangka Raya

The electronic cigarette (e-cigarette) is one of the Nicotine Replacement Therapy uses electricity from battery power to deliver nicotine as aerosols. Nicotine can stimulate secretion of catecholamine lead to increase secretion of hepatic triglycerides to the blood vessels. This study aimed to asses the triglyceride levels of electronic cigarette smokers (also known as vapers) on a vaporizer community in Pahandut, Palangka Raya, Central Kalimantan. The cross-sectional design was carried out to asses triglyceride levels on 29 electronic cigarette users. Triglyceride assay was determined by an automated photometric technique using Photometers (5010 V5+ Robert Riele). This study found that mostly e-cigarette user considered normal triglyceride level as 75.9% with average triglyceride level 100.6 mg/dL, 13.8% considered borderline-high triglyceride level with average triglyceride level 180.8 mg/dL, and only 10.3% considered high triglyceride level with average triglyceride level 293.0 mg/dL.

Cholinesterase Activity on Palm Oil Plantation Workers in Parenggean, Kotawaringin Timur

Use of pesticide is widely used in palm oil plantation industry. Pesticide exposure can affect pesticide-spraying workers' health. This study aimed to determine acetylcholinesterase enzyme (AChE) activity levels and identify of poisoning risk factors on pesticide-spraying workers in a palm oil plantation Comanditaire Venootschap (CV) unit in Parenggeran, Kotawaringin Timur, Central Kalimantan. The cross-sectional study design was conducted to examine cholinesterase enzyme activity levels on 15 pesticide-spraying workers. The cholinesterase levels were measured using an automated photometric technique. This study found that all workers have normal cholinesterase activity levels. The average cholinesterase activity levels were 7065,36 U/L and 6528 U/L, respectively for male and female workers. It was also found that two male workers cholinesterase activity levels of using glyphosate pesticide were almost close to the lower limit of the normal levels range, 4720 U/L, and 4958 U/L, respectively. Use of Personal Protective Equipment (PPE) during the pesticide spraying and maximum time allowed of pesticide spraying in a day have to be considered as an important rule to avoid excessive pesticide exposure.