Immunohistochemical Localization of Melanoma-Associated Antigen p94 kd200 with the Use of a Modified Avidin-Biotin-Complex Lectin Method

1987 ◽  
Vol 73 (5) ◽  
pp. 451-455
Author(s):  
Sylvia Garnis-Jones ◽  
David McLean ◽  
Ann Worth
Keyword(s):  
Antigen Expression ◽  
Immunohistochemical Localization ◽  
Lentigo Maligna ◽  
Blue Nevus ◽  
Superficial Spreading ◽  
Clin Pathol ◽  
Superficial Spreading Melanoma ◽  
Dysplastic Nevus ◽  
Dysplastic Nevi ◽  
Avidin Biotin Complex

The immunohistochemical localization of melanoma-associated antigen p94 kd200 was investigated in frozen sections of 3 congenital nevi, 4 benign intradermal nevi, 1 regressing nevus, 1 blue nevus, 1 dysplastic nevus, 1 lentigo maligna, 1 superficial spreading melanoma and 2 metastatic melanomas. The original avidin-biotin complex lectin method (Hsu SM, Raine L, Fanger H: Am. J. Clin. Pathol., 75: 734–738, 1981) was modified to detect the antigen. The sections were exposed to the monoclonal antibody to p94 kd200 (Hybritech Inc.), the linking biotin-labelled anti-mouse IgG, the avidin-biotin peroxidase complex and the 3-amino-9-ethylcarbazole solution in an incubator at 37 °C and 100% humidity. We found that the percentage of cells expressing p94 kd200 varied between 0 and 100% in congenital nevi, between 80 and 100% in benign intradermal nevi, between 0 and 20% in the regressing, blue and dysplastic nevi, and in the lentigo maligna, 80 to 100% in the superficial spreading melanoma, and between 0 and 40% in the metastatic melanomas. Positive cells were found to be hypomelanotic (did not have heavy melanin content). The intensity of labelling or the degree of antigen expression on benign and malignant hypomelanotic cells was also found to vary. These findings 1) reinforce the concept of quantitative rather than qualitative antigenic differences in benign and malignant cells 2) suggest that kd200 is lost with increasing pigment production 3) offer a potentially significant tool to investigate the antigenic changes during cell differentiation.

Lentigo Maligna and Superficial Spreading Melanoma Differ Biologically In Their In Situ Phase.

1999 ◽  
Vol 21 (1) ◽  
pp. 72
Author(s):  
A. Barzilai ◽  
S. Auslander ◽  
I. Goldberg ◽  
J. Kopolovic ◽  
H. Trau
Keyword(s):  
Lentigo Maligna ◽  
Superficial Spreading ◽  
Superficial Spreading Melanoma

Lentigo maligna and superficial spreading melanoma are different in their in situ phase: An immunohistochemical study

2002 ◽  
Vol 33 (10) ◽  
pp. 1001-1005 ◽  
Author(s):  
Silviu Auslender ◽  
Aviv Barzilai ◽  
Iris Goldberg ◽  
Juri Kopolovic ◽  
Henri Trau
Keyword(s):  
Immunohistochemical Study ◽  
Lentigo Maligna ◽  
Superficial Spreading ◽  
Superficial Spreading Melanoma

Long Term Results of Surgical Treatment of Melanoma of the Limbs

1976 ◽  
Vol 62 (2) ◽  
pp. 233-242 ◽  
Author(s):  
Natale Cascinelli ◽  
Gian Paolo Balzarini ◽  
Vincenzo Fontana ◽  
Alberto Morabito ◽  
Sergio Orefice
Keyword(s):  
Surgical Treatment ◽  
Primary Melanoma ◽  
Long Term Results ◽  
Depth Of Invasion ◽  
Node Dissection ◽  
Lentigo Maligna ◽  
Superficial Spreading ◽  
Superficial Spreading Melanoma ◽  
Nodular Melanoma

The outcome of surgical treatment of malignant melanoma has been evaluated on the basis of 157 limbs patients observed at the National Cancer Institute of Milan from January 1950 to December 1973. It was found that sex, site of origin and excisional biopsy do not affect the prognosis. The presence of regional nodes metastases is the factor that weight most heavily on long term results: 57.6% of patients without regional node metastases and only 15.2% of patients with positive bodes are free of disease 10 years after treatment. The depth of invasion and histological type of melanoma were found to be useful because they reveal the potential aggressiveness of the tumor: 2 out of 11 cases of lentigo maligna, 4 out of 55 cases of superficial spreading melanoma, 23 out of 91 cases of nodular melanoma and 12 out of 79 cases of Clark's level III, 8 out of 44 level IV and 8 out of 14 level V had regional nodes metastases. These two parameters were found to be correlated: lentigo maligna and superficial spreading melanoma infiltrate little as a rule whereas about half of nodular melanoma were classified as levels IV or V. Since there is not at present a definite evidence that an « elective » node dissection achieves better risults than excision only of primary melanoma followed by a possible « curative » dissection the so called « prophilactic » node dissection is considered mainly as a « staging » procedure and indicated only for level V melanoma where the incidence of nodes metastases is higher than 50%.

scholarly journals Evolution of superficial spreading melanoma to resemble desmoplastic melanoma: case report

2021 ◽  
Author(s):  
Martin G. Cook ◽  
Barry W. E. M. Powell ◽  
Megan E. Grant ◽  
Adele C. Green
Keyword(s):  
Case Report ◽  
Clinical Management ◽  
Unusual Case ◽  
Primary Site ◽  
Mixed Tumor ◽  
Superficial Spreading ◽  
Superficial Spreading Melanoma ◽  
Desmoplastic Melanoma ◽  
Mixed Features ◽  
Melanoma Case

AbstractDesmoplastic melanoma commonly occurs on the head and neck in a pure form, but occasionally, it occurs in a mixed tumor with another type, usually superficial spreading melanoma (SSM), and rarely as a metastasis from a primary SSM. We report here a primary SSM on the leg of a 32-year-old male which metastasised to lymph nodes, and 10 years later recurred at the primary site initially with mixed features but evolving to resemble a uniformly desmoplastic, deeply invasive melanoma. This unusual case has implications for clinical management and is additionally notable for its reversal in behavior, from metastatic to local infiltrative type, correlating with the change in morphology.

scholarly journals Cutaneous Melanomas Arising during Childhood: An Overview of the Main Entities

2021 ◽  
Vol 8 (3) ◽  
pp. 301-314
Author(s):  
Arnaud de la Fouchardière ◽  
Felix Boivin ◽  
Heather C. Etchevers ◽  
Nicolas Macagno
Keyword(s):  
Lymph Node ◽  
Regional Lymph Node ◽  
Germline Mutations ◽  
Epithelioid Cells ◽  
Superficial Spreading ◽  
Superficial Spreading Melanoma ◽  
Cancer Predisposition Syndrome ◽  
Specific Morphology ◽  
Genetic Features ◽  
Molecular Assessment

Cutaneous melanomas are exceptional in children and represent a variety of clinical situations, each with a different prognosis. In congenital nevi, the risk of transformation is correlated with the size of the nevus. The most frequent type is lateral transformation, extremely rare before puberty, reminiscent of a superficial spreading melanoma (SSM) ex-nevus. Deep nodular transformation is much rarer, can occur before puberty, and must be distinguished from benign proliferative nodules. Superficial spreading melanoma can also arise within small nevi, which were not visible at birth, usually after puberty, and can reveal a cancer predisposition syndrome (CDKN2A or CDK4 germline mutations). Prognosis is correlated with classical histoprognostic features (mainly Breslow thickness). Spitz tumors are frequent in adolescents and encompass benign (Spitz nevus), intermediate (atypical Spitz tumor), and malignant forms (malignant Spitz tumor). The whole spectrum is characterized by specific morphology with spindled and epithelioid cells, genetic features, and an overall favorable outcome even if a regional lymph node is involved. Nevoid melanomas are rare and difficult to diagnose clinically and histologically. They can arise in late adolescence. Their prognosis is currently not very well ascertained. A small group of melanomas remains unclassified after histological and molecular assessment.

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