Value, Limitations, and Recommendations for Grading of Canine Cutaneous Mast Cell Tumors: A Consensus of the Oncology-Pathology Working Group

2021 ◽  
pp. 030098582110097
Author(s):  
Davide Berlato ◽  
Julie Bulman-Fleming ◽  
Craig A. Clifford ◽  
Laura Garrett ◽  
Joanne Intile ◽  
...  
Keyword(s):  
Mast Cell ◽  
Current Literature ◽  
Critical Review ◽  
Working Group ◽  
Relevant Information ◽  
Cancer Society ◽  
Clinical Interpretation ◽  
Histologic Grading ◽  
Mast Cell Tumors ◽  
Cutaneous Mast Cell

One of the primary objectives of the Oncology Pathology Working Group (OPWG), a joint initiative of the Veterinary Cancer Society and the American College of Veterinary Pathologists, is for oncologists and pathologists to collaboratively generate consensus documents to standardize aspects and provide guidelines for oncologic pathology. Consensus is established through review of relevant peer-reviewed literature relative to a subgroup’s particular focus. In this article, the authors provide a critical review of the current literature for grading of canine cutaneous mast cell tumors, suggest guidelines for reporting, and provide recommendations for its clinical interpretation. The article mainly focuses on histologic grading, but relevant information on mitotic count and cytological grading are also discussed. This document represents the opinions of the working group and the authors but does not constitute a formal endorsement by the American College of Veterinary Pathologists or the Veterinary Cancer Society.

scholarly journals Variation among Pathologists in the Histologic Grading of Canine Cutaneous Mast Cell Tumors with Uniform Use of a Single Grading Reference

2005 ◽  
Vol 17 (6) ◽  
pp. 561-564 ◽  
Author(s):  
N. C. Northrup ◽  
E. W. Howerth ◽  
B. G. Harmon ◽  
C. A. Brown ◽  
K. P. Carmicheal ◽  
...  
Keyword(s):  
Mast Cell ◽  
Histologic Grading ◽  
Mast Cell Tumors ◽  
Cutaneous Mast Cell ◽  
Is Value

Ten veterinary pathologists independently assigned histologic grades to the same 60 canine cutaneous mast cell tumors using the Patnaik classifications. The degree of agreement in grading among the pathologists was compared with the degree of agreement among the same pathologists in a previous study, in which each pathologist used the reference for grading that he/she uses routinely. Mean agreement improved significantly from 50.3% to 62.1% with uniform use of the Patnaik classifications ( P = 0.00001), suggesting that there is value in uniform application of a single grading scheme for canine cutaneous mast cell tumors. Agreement among pathologists was still not 100%, suggesting that a more objective grading scheme should be developed and that other histologic indicators of prognosis should be investigated.

scholarly journals Variation among Pathologists in Histologic Grading of Canine Cutaneous Mast Cell Tumors

2005 ◽  
Vol 17 (3) ◽  
pp. 245-248 ◽  
Author(s):  
Nicole C. Northrup ◽  
Barry G. Harmon ◽  
Tracy L. Gieger ◽  
Cathy A. Brown ◽  
K. Paige Carmichael ◽  
...  
Keyword(s):  
Mast Cell ◽  
Significant Variation ◽  
Low Grade ◽  
Histologic Grading ◽  
Mast Cell Tumors ◽  
Cutaneous Mast Cell ◽  
Mitotic Figures

Ten veterinary pathologists at 1 veterinary institution independently assigned histologic grades to the same 60 canine cutaneous mast cell tumors (MCTs). There was significant variation among pathologists in grading the MCTs ( P < 0.001). The probability of assigning a low grade was significantly higher for the pathologists in this study who use a published reference for histologic grading of canine cutaneous MCTs that allows subcutaneous MCTs or MCTs with mitotic figures to be included in the low-grade category ( P < 0.0001 and P < 0.0001, respectively).

Letter to the Editor re Hergt et al “Use of a 2-tier histologic grading system for canine cutaneous mast cell tumors on cytology specimens”

2016 ◽  
Vol 45 (4) ◽  
pp. 531-531
Author(s):  
Melinda Camus ◽  
Heather Priest ◽  
Jennifer Koehler ◽  
Elizabeth Driskell ◽  
Marcia Ilha ◽  
...  
Keyword(s):  
Mast Cell ◽  
Grading System ◽  
Histologic Grading ◽  
Mast Cell Tumors ◽  
Letter To The Editor ◽  
Cutaneous Mast Cell

Use of a 2-tier histologic grading system for canine cutaneous mast cell tumors on cytology specimens

2016 ◽  
Vol 45 (3) ◽  
pp. 477-483 ◽  
Author(s):  
Franziska Hergt ◽  
Wolf von Bomhard ◽  
Michael S. Kent ◽  
Johannes Hirschberger
Keyword(s):  
Mast Cell ◽  
Grading System ◽  
Histologic Grading ◽  
Mast Cell Tumors ◽  
Cutaneous Mast Cell

scholarly journals Proposal of a 2-Tier Histologic Grading System for Canine Cutaneous Mast Cell Tumors to More Accurately Predict Biological Behavior

2010 ◽  
Vol 48 (1) ◽  
pp. 147-155 ◽  
Author(s):  
M. Kiupel ◽  
J. D. Webster ◽  
K. L. Bailey ◽  
S. Best ◽  
J. DeLay ◽  
...  
Keyword(s):  
Mast Cell ◽  
Biological Behavior ◽  
Grading System ◽  
Histologic Grading ◽  
Mast Cell Tumors ◽  
Cutaneous Mast Cell

scholarly journals Stereology in Grading and Prognosis of Canine Cutaneous Mast Cell Tumors

2021 ◽  
pp. 030098582098513
Author(s):  
Mafalda Casanova ◽  
Sandra Branco ◽  
Inês Berenguer Veiga ◽  
André Barros ◽  
Pedro Faísca
Keyword(s):  
Mast Cell ◽  
Clinical Outcome ◽  
Prognostic Value ◽  
Histological Grade ◽  
Intraobserver Variability ◽  
Low Grade ◽  
High Grade ◽  
Mast Cell Tumors ◽  
Cutaneous Mast Cell ◽  
Grade Iii

Canine cutaneous mast cell tumors (ccMCTs) are currently graded according to Patnaik and Kiupel grading schemes. The qualitative and semiquantitative parameters applied in these schemes may lead to inter- and intraobserver variability. This study investigates the prognostic value of volume-weighted mean nuclear volume ([Formula: see text]), a stereological estimation that provides information about nuclear size and its variability. [Formula: see text] of 55 ccMCTs was estimated using the “point-sampled intercept” method and compared with histological grade and clinical outcome. The clinical history of dogs treated with surgical excision alone was available for 30 ccMCTs. Statistical differences in [Formula: see text] were found between grade II ([Formula: see text]= 115 ± 29 µm3) and grade III ccMCTs ([Formula: see text]= 197 ± 63 µm3), as well as between low-grade ([Formula: see text]= 113 ± 28 µm3) and high-grade ccMCTs ([Formula: see text]= 184 ± 63 µm3). An optimal cutoff value of [Formula: see text] ≥ 150 µm3 and [Formula: see text] ≥ 140 µm3 was determined for grade III and high-grade ccMCTs, respectively. In terms of prognosis, [Formula: see text] was not able to predict the clinical outcome in 42% of the cases; however, cases with [Formula: see text]<125 µm3 had a favorable outcome. These results indicate that, despite having limited prognostic value when used as a solitary parameter, [Formula: see text] is highly reproducible and is associated with histological grade as well as with benign behavior.

Correlation of histologic grading of canine mast cell tumors with Ki67/PCNA/AgNOR/c-Kit scores: 38 cases (2002-2003)

2004 ◽  
Vol 2 (2) ◽  
pp. 98-98 ◽  
Author(s):  
P. J. Bergman ◽  
D. M. Craft ◽  
S. J. Newman ◽  
K. Baer ◽  
M. A. Camps-Palau ◽  
...  
Keyword(s):  
Mast Cell ◽  
Histologic Grading ◽  
Mast Cell Tumors

scholarly journals Ancillary techniques on the evaluation of canine cutaneous mast cell tumors from Brazil

2016 ◽  
Vol 46 (10) ◽  
pp. 1804-1810 ◽  
Author(s):  
Mariana Martins Flores ◽  
Renata Dalcol Mazaro ◽  
Ingeborg Maria Langohr ◽  
Alma Roy ◽  
Keith Strother ◽  
...  
Keyword(s):  
Mast Cell ◽  
Pcr Amplification ◽  
The United States ◽  
Growth Fraction ◽  
Grading System ◽  
Low Grade ◽  
Ki 67 ◽  
Mast Cell Tumors ◽  
Cutaneous Mast Cell ◽  
Exon 11

ABSTRACT: The use of histologic classification by a 2-tier grading system only, immunohistochemistry (IHC) for KIT and Ki-67 and polymerase chain reaction (PCR) for internal tandem duplications (ITD) on exon 11 has improved the prognostication of canine cutaneous mast cell tumors (CCMTs) particularly in the United States. However, these techniques are not commonly used in most Brazilian laboratories. Likewise, no studies, to date, have investigated the occurrence of ITD in CCMTs from the country. Thus, this study tested the 2-tier grading system, the immunohistochemistry for KIT and Ki-67 and the PCR for exon 11 in a group of Brazilian CCMTs with the goal of investigating the applicability of these tests in a Brazilian laboratory. Of the 39 CCMTs, 69.2% (27/39) were identified as low-grade and 30.8% (12/39) as high-grade by a 2-tier grading system. All tumors had a KIT expression pattern II, and 30.6% (11/36) had a high growth fraction (Ki-67). PCR amplification was successful in four of the 11 tumors examined. Two of these (50%) were positive for ITD. This study highlights the importance of using auxiliary techniques in the CCMT evaluation, identifies limitations and confirms the applicability of these methods on a routine diagnostic basis in Brazil. Our results will help to improve the prognostication of CCMTs in Brazilian diagnostic laboratories, encouraging the use of supplementary methods.

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