Mouse Hepatoblastomas: A Histologic, Ultrastructural, and Immunohistochemical Study

Hepatoblastomas from B6C3F1 and BALB/c mice were examined by light and electron microscopy and by immunohistochemical reactions for alpha-fetoprotein, keratin, and vimentin. Tumors occurred in one group of a chronic bioassay for the interaction of diet, genetic strain, and the carcinogen, 2-acetylaminofluorene. Tumors had several populations (including epithelial and mesenchymal cells) in various stages of differentiation. Neoplastic epithelial cells had features of embryonal hepatocytes, such as sparse cytoplasmic organelles, absence of glycogen, abundant free ribosomes, occasional bile canaliculi, and peroxisome-like dense bodies. Embryonal fibroblast-like cells had pleomorphic and folded nuclei with prominent perinuclear chromatin and dispersed cytoplasmic organelles. Fibroblast-like cells were surrounded by bundles of collagen fibrils. Intermediate or transitional types of cells were seen. No tumor cells were immunoreactive for mouse alpha-fetoprotein (AFP) antibody, unlike those in hepatocellular adenomas or carcinomas. Epithelial and mesenchymal tumor cells contained intermediate filaments throughout the cytoplasm; some of these cells stained for keratin but not for vimentin. These findings suggest that mouse hepatoblastomas are derived from bipotential liver blastema cells and are composed of a mixture of several cell populations.

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A Case of Iris Melanocytoma Demonstrating Diffuse Melanocytic Proliferation with Uncontrolled Intraocular Pressure

Case Reports in Ophthalmology ◽

10.1159/000464349 ◽

2017 ◽

Vol 8 (1) ◽

pp. 190-194 ◽

Cited By ~ 2

Author(s):

Mami Kusunose ◽

Yuji Sakino ◽

Yoshihiro Noda ◽

Tsutomu Daa ◽

Toshiaki Kubota

Keyword(s):

Electron Microscopy ◽

Intraocular Pressure ◽

Tumor Cells ◽

Trabecular Meshwork ◽

Light And Electron Microscopy ◽

Secondary Glaucoma ◽

Pigment Dispersion ◽

Large Tumor ◽

History Of ◽

Ring Melanoma

We report a rare case with histologically proven melanocytoma of the iris that demonstrated diffuse melanocytic proliferation with uncontrolled secondary glaucoma and investigate the etiology of the intraocular pressure elevation. The patient was a 78-year-old man with a history of darkened iris of his left eye. The intraocular pressure was 39 mm Hg. A slit-lamp examination showed a diffuse darkened iris, and a gonioscopic examination revealed open angle with circumferential heavy pigmentation. There was no pigment dispersion of the anterior chamber and no pigment deposition of the cornea. We suspected malignant ring melanoma in the left eye and enucleated it. The globe was examined with light and electron microscopy. Light microscopy revealed the presence of heavily pigmented tumor cells in the iris, ciliary body, trabecular meshwork, and Schlemm’s canal. A bleached preparation showed large tumor cells with central and paracentral nuclei without mitosis. Electron microscopy of the trabecular meshwork revealed melanin-bearing tumor cells invading the intertrabecular spaces, and the melanin granules were not phagocytosed in the trabecular cells. The mechanical obstruction of the aqueous flow by the tumor cells may be a major cause of secondary glaucoma in eyes with iris melanocytoma presenting diffuse proliferation.

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Osteogenic Sarcoma of the Breast. A Case Report

Tumori Journal ◽

10.1177/030089169207800110 ◽

1992 ◽

Vol 78 (1) ◽

pp. 43-46 ◽

Cited By ~ 8

Author(s):

Simonetta Bianchi ◽

Giorgio Malatantis ◽

Gaetano Cardona ◽

Giancarlo Zampi

Keyword(s):

Electron Microscopy ◽

Case Report ◽

Immunohistochemical Study ◽

Light And Electron Microscopy ◽

Osteogenic Sarcoma ◽

Epithelial Differentiation ◽

Osseous Metaplasia ◽

Ultrastructural Studies

A case of primary osteogenic sarcoma of the breast is reported. It should be distinguished from carcinoma with extensive osseous metaplasia. The results of light and electron microscopy including an immunohistochemical study are presented. Immunohistochemical and ultrastructural studies proved that the lesion, in the absence of epithelial differentiation, was a primary osteogenic sarcoma of the breast rather than a carcinoma with extensive osseous metaplasia. Diagnosis may be delayed because the tumor is confused clinically and mammographically with a calcific fibroadenoma.

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TRANSMISSION ELECTRON MICROSCOPY OF FUNGAL NEMATODE-TRAPPING DEVICES

Canadian Journal of Plant Science ◽

10.4141/cjps79-121 ◽

1979 ◽

Vol 59 (3) ◽

pp. 785-795 ◽

Cited By ~ 7

Author(s):

S. S. TZEAN ◽

R. H. ESTEY

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Cytoplasmic Organelles ◽

Dense Bodies ◽

Transmission Electron ◽

Luminal Side ◽

Septal Pores ◽

Trapping Devices

The nematode-trapping devices of Arthrobotrys dactyloides (constricting rings), Monacrosporium cionopagum (adhesive columnar processes and scalariform loops) and a Dactylella sp. (sticky knobs) were investigated by electron microscopy. The cells of the constricting rings prior to inflation contained normal cytoplasmic organelles and some unusual, oblong, electron-dense inclusions in the luminal side of the protoplast, and lomasomes associated with papillate cylindrical bodies in the peripheral side. Their luminal walls differed from their peripheral walls in structure and thickness. After inflation, the ring cells had thinner luminal walls, the electron-dense inclusions were absent, there were fewer lomasomes, the cells had larger vacuoles, some of which contained electron-dense fine granules, and Woronin bodies were plugging the septal pores. It is postulated that the cells of constricting rings are inflated by means of rapidly generated gases rather than by an inflow of fluids. The sticky knob, adhesive columnar process, and scalariform loop trapping devices exhibited numerous globose electron-dense bodies, especially in their peripheral protoplasts.

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Correlative Light and Electron Microscopy of Rare Cell Populations in Zebrafish Embryos Using Laser Marks

Zebrafish ◽

10.1089/zeb.2015.1148 ◽

2015 ◽

Vol 12 (6) ◽

pp. 470-473 ◽

Cited By ~ 2

Author(s):

Mohammad Goudarzi ◽

Karina Mildner ◽

Felix Babatz ◽

Dietmar Riedel ◽

Christian Klämbt ◽

...

Keyword(s):

Electron Microscopy ◽

Light And Electron Microscopy ◽

Cell Populations ◽

Zebrafish Embryos

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Microscopic and cytochemical characterisation of haemocytes of the mud crab Scylla serrata (Forskål, 1775) (Decapoda, Portunidae)

Crustaceana ◽

10.1163/15685403-00003226 ◽

2013 ◽

Vol 86 (10) ◽

pp. 1234-1249 ◽

Cited By ~ 1

Author(s):

Brajendu Kumar ◽

A. Deepika ◽

M. Arumugam ◽

P. Mullainadhan ◽

M. Makesh ◽

...

Keyword(s):

Electron Microscopy ◽

Toluidine Blue ◽

Light And Electron Microscopy ◽

Morphological Features ◽

Scylla Serrata ◽

Mud Crab ◽

Cytoplasmic Organelles ◽

Well Differentiated ◽

Total Haemocyte Count ◽

Prophenol Oxidase

Haemocytes of the mud crab Scylla serrata (Forskål, 1775) were characterised based on morphological features using light and electron microscopy, and cytochemistry. The cells were identified as hyaline, semigranular and granular haemocytes. Hyaline cells were the smallest haemocytes among the three types identified, having the highest nucleo-cytoplasmic ratio. The cells showed a number of cytoplasmic organelles and also contained a few small as well as large-sized granules. Semigranular haemocytes possessed moderate numbers of large-sized granules or numerous small-sized granules and comparatively less numbers of organelles. Granular haemocytes were the largest haemocytes with the lowest nucleo-cytoplasmic ratio and contained many large-sized granules. Cytoplasmic organelles were least observed in the granular haemocytes. These three haemocyte morphotypes constituted 60, 21 and 19%, respectively, of the total haemocyte population, while the total haemocyte count was 7.31 × 106 to 7.18 × 107 with a mean of 2.86 × 107 cells ml−1. In cytochemical studies performed to localize carbohydrates, lipids and prophenol oxidase, all the haemocyte types were positive for PAS and toluidine blue, indicating the presence of mucopolysaccharides, whereas semigranular and granular haemocytes were rich in carbohydrates and lipid moieties. Besides, prophenol oxidase was localised within the granules of semigranular and granular haemocytes. Hyaline haemocytes showed an abundance of well differentiated cytoplasmic organelles and granules, and there was a distinct differentiation between semigranular and granular haemocytes in terms of granules and organelles. This is the first report of the characterisation of haemocytes of the mud crab.

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Aspects ultrastructuraux et cytochimiques des hématies nucléées de deux Annélides Polychètes Notomastus latericeus Sars et Glycera convoluta Keferstein

Canadian Journal of Zoology ◽

10.1139/z80-083 ◽

1980 ◽

Vol 58 (4) ◽

pp. 589-597 ◽

Cited By ~ 2

Author(s):

K. E. Sean ◽

B. Boilly

Keyword(s):

Electron Microscopy ◽

Iron Content ◽

Light And Electron Microscopy ◽

Cytoplasmic Organelles ◽

Glycera Convoluta

The structure and cytochemistry (pseudoperoxidase activity and iron content) of the nucleated erythrocytes of two polychaetes, Notomastus latericeus Sars and Glycera convoluta Keferstein, have been investigated using light and electron microscopy. These cells contain few cytoplasmic organelles but do possess iron-rich structures in vacuoles and free in the cytoplasm. Both species show a pseudoperoxidase activity throughout the cytoplasm and, in the case of Notomastus, in the nucleus as well. The cytological and cytochemical aspects of these cells suggest a low metabolism and a limited lifetime, particularly in Notomastus.

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Fine Structure of a Transplantable Reticulum Cell Sarcoma. I. Light and Electron Microscopy of Viable and Necrotic Tumor Cells2

JNCI Journal of the National Cancer Institute ◽

10.1093/jnci/34.4.459 ◽

1965 ◽

Keyword(s):

Electron Microscopy ◽

Fine Structure ◽

Tumor Cells ◽

Light And Electron Microscopy ◽

Reticulum Cell ◽

Reticulum Cell Sarcoma ◽

Cell Sarcoma ◽

Necrotic Tumor

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Langerhans’ Granules in Cells of an Eosinophilic Histiocytoma

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010010072x ◽

1968 ◽

Vol 26 ◽

pp. 196-197

Author(s):

John H. L. Watson ◽

John L. Swedo ◽

A. R. Morales

Keyword(s):

Electron Microscopy ◽

Tumor Cells ◽

Present Report ◽

Normal Skin ◽

Uranyl Acetate ◽

Dense Bodies ◽

Lead Hydroxide ◽

Membrane Bound ◽

Numerous Microvillus ◽

In Cells

Recent papers describe Langerhans’ granules in cells of normal skin, in Letterer-Siwe disease, and associated with osseous and pulmonary lesions of histiocytosis X. The present report concerns the structure and occurrence of these granules in a solitary tumor of the human posterior anal wall, diagnosed as an eosinophilic histiocytoma, confirmed by the electron microscopy. The investigation supports the hypothesis that the epidermal Langerhans’ cell is a macrophage. Samples of the recurrent tumor were fixed in cacodylate-buffered 6.4% glutaraldehyde, washed in cacodylate-buffered 0.2M sucrose, and postfixed in OSO4 for electron microscopy. Embedment was in Araldite, and sections were contrasted with uranyl acetate and lead hydroxide.The surface of the tumor cells was very irregular, with numerous microvillus-like projections, and was without both desmosomes and basement membrane. The nuclei were lobulated, and possessed both finely dispersed and coagulated chromatin, with multiple nucleoli. The system of parallel tubules and sacs which composed the Golgi was prominent in most cells. Numerous linear profiles of RER occurred. The RNA particles were also abundant throughout the cytoplasm in groups unrelated to the RER. Centrioles were seen frequently paranuclearly. The mitochondria, with well developed cristae, were frequently altered, being locally swollen, vacuolated or containing myelin figures, Fig. A. The most striking organelles were (i) the Langerhans’ granules (L), observed in abundance throughout the cytoplasm of most of the tumor cells, but predominantly in those cells which contained (ii) the fusiform or ovoid, membrane-bound, electron-dense bodies (B). Many of these rounded, dense bodies contained concentric, double-membranous structures, Figs. C and D.

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Immuno-correlative light and electron microscopy (iCLEM) using SEM v1

10.17504/protocols.io.bu54ny8w ◽

2021 ◽

Author(s):

Viola Oorschot ◽

Jillian C Danne ◽

Benjamin Lindsey ◽

Jan Kaslin ◽

Georg Ramm

Keyword(s):

Electron Microscopy ◽

Light And Electron Microscopy ◽

High Resolution Electron Microscopy ◽

Cell Populations ◽

Tissue Sections ◽

Resolution Electron ◽

Stem And Progenitor Cells ◽

Scanning Em ◽

Nerve System ◽

Cytochemical Markers

Immuno- correlative light and electron microscopy (iCLEM) combines ultrastructural information obtained from high resolution electron microscopy with the use of genetically encoded or cytochemical markers. Immuno-CLEM takes advantage of the antigenicity preserved by Tokuyasu sample preparation to identify, quantify and characterise heterogeneous cell populations in small organisms, organs and tissue of healthy and diseased states. iCLEM can be used in combination with scanning EM (SEM), scanning TEM (STEM), and transmission EM (TEM). These protocols are well-suited, for example, for investigating neural stem and progenitor cell populations of the vertebrate nerve system and are available as separate protocols on protocol.io. Here, a method for iCLEM-SEM is described using an adult zebrafish telencephalon brain as a model. This organ is small in size allowing the complete dorsal telencephalic niche to be visualised in sections, and has diverse cell profiles and regenerative potential of local neural stem and progenitor cells. iCLEM-SEM provides a large quantifiable overview of 200 nm tissue sections without the presence of grid bars, and thicker sections enhance the immunofluorescent labelling.

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