Langerhans’ Granules in Cells of an Eosinophilic Histiocytoma

1968 ◽  
Vol 26 ◽  
pp. 196-197
Author(s):  
John H. L. Watson ◽  
John L. Swedo ◽  
A. R. Morales
Keyword(s):  
Electron Microscopy ◽  
Tumor Cells ◽  
Present Report ◽  
Normal Skin ◽  
Uranyl Acetate ◽  
Dense Bodies ◽  
Lead Hydroxide ◽  
Membrane Bound ◽  
Numerous Microvillus ◽  
In Cells

Recent papers describe Langerhans’ granules in cells of normal skin, in Letterer-Siwe disease, and associated with osseous and pulmonary lesions of histiocytosis X. The present report concerns the structure and occurrence of these granules in a solitary tumor of the human posterior anal wall, diagnosed as an eosinophilic histiocytoma, confirmed by the electron microscopy. The investigation supports the hypothesis that the epidermal Langerhans’ cell is a macrophage. Samples of the recurrent tumor were fixed in cacodylate-buffered 6.4% glutaraldehyde, washed in cacodylate-buffered 0.2M sucrose, and postfixed in OSO4 for electron microscopy. Embedment was in Araldite, and sections were contrasted with uranyl acetate and lead hydroxide.The surface of the tumor cells was very irregular, with numerous microvillus-like projections, and was without both desmosomes and basement membrane. The nuclei were lobulated, and possessed both finely dispersed and coagulated chromatin, with multiple nucleoli. The system of parallel tubules and sacs which composed the Golgi was prominent in most cells. Numerous linear profiles of RER occurred. The RNA particles were also abundant throughout the cytoplasm in groups unrelated to the RER. Centrioles were seen frequently paranuclearly. The mitochondria, with well developed cristae, were frequently altered, being locally swollen, vacuolated or containing myelin figures, Fig. A. The most striking organelles were (i) the Langerhans’ granules (L), observed in abundance throughout the cytoplasm of most of the tumor cells, but predominantly in those cells which contained (ii) the fusiform or ovoid, membrane-bound, electron-dense bodies (B). Many of these rounded, dense bodies contained concentric, double-membranous structures, Figs. C and D.

Problems in Myogenesis Terminology in Electron Microscopy

1967 ◽  
Vol 25 ◽  
pp. 38-39
Author(s):  
P.E. Conen ◽  
J.U. Balis ◽  
C.D. Bell
Keyword(s):  
Electron Microscopy ◽  
Cell Types ◽  
Uranyl Acetate ◽  
Electron Microscopic ◽  
Accurate Identification ◽  
Microscopic Studies ◽  
A Cell ◽  
Lead Hydroxide ◽  
Developing Muscle

Myogenesis in man was studied using muscle from 19 fetuses of 8 to 16 weeks gestation which were processed with standard osmium-Epon or glutaraldehyde-osmium-Epon schedules and sections were stained in uranyl acetate and/or lead hydroxide. Particular emphasis was given during this study to presence of basement membrane and myofilaments as additional aids in classification of cell types present in developing muscle.Electron microscopy permits accurate identification of fibroblasts and early cells of muscle series and has been used in studies of myogenesis in chick, and rat. Light microscopy definitions for premyoblasts and myoblasts, and for myocytes at the myotube and muscle fiber stages of development are difficult to apply to electron microscopic studies without modification. For example the term myoblast was used differently by Tello, Katznelson and Boyd to designate a cell destined to become muscle but not recognizable as a muscle cell.

Cytopathology and ultrastructure of mild and severe strains of maize chlorotic dwarf virus in maize and johnsongrass

1993 ◽  
Vol 71 (5) ◽  
pp. 718-724 ◽  
Author(s):  
E. D. Ammar ◽  
R. E. Gingery ◽  
L. R. Nault
Keyword(s):  
Electron Microscopy ◽  
Uranyl Acetate ◽  
Dwarf Virus ◽  
Mesophyll Cells ◽  
White Stripe ◽  
Cytoplasmic Inclusions ◽  
Maize Leaves ◽  
Maize Chlorotic Dwarf Virus ◽  
In Cells

In maize leaves experimentally infected with various isolates or strains of maize chlorotic dwarf virus, including a newly characterized strain (M1), and in naturally infected johnsongrass, only two types of cytoplasmic inclusions were consistently observed: (i) quasi-spherical electron-dense granular inclusions, and (ii) curved or straight bundles of fibrous inclusions. Both types were detected by light and (or) electron microscopy in vascular parenchyma and phloem cells, and less frequently in bundle-sheath and adjacent mesophyll cells. The dense granular inclusions usually contained numerous isometric virus-like particles, some of which may have been released into the surrounding cytoplasm. However, a high proportion of these inclusions in cells infected with the mild type strain and a type-like isolate (M8) were either devoid of or contained very few viruslike particles. In maize leaves infected with the white stripe (WS) isolate, the chloroplasts were markedly deformed; in leaves of stunted plants doubly infected with M8 and the serologically distinct M1 strain, some phloem cells appeared degenerated. Electron microscopy of preparations of purified M1 stained with uranyl acetate revealed both stain-impenetrable full particles and stain-penetrable empty or partially empty particles. Both full and apparently empty particles were also found in cells of maize leaves infected with M1, whereas with other strains and isolates, mainly full particles were found both in situ and in vitro. Key words: maize chlorotic dwarf virus, cytopathology, ultrastructure, maize, johnsongrass.

scholarly journals HALE STAIN FOR SIALIC ACID-CONTAINING MUCINS

1963 ◽  
Vol 19 (1) ◽  
pp. 223-228 ◽  
Author(s):  
Gabriel Gasic ◽  
Leonard Berwick
Keyword(s):  
Electron Microscopy ◽  
Sialic Acid ◽  
Tumor Cells ◽  
Potassium Permanganate ◽  
Ferric Oxide ◽  
Uranyl Acetate ◽  
Potassium Ferrocyanide ◽  
Ascites Tumor ◽  
Mouse Ascites

The feasibility of using the Hale stain to identify cellular sialic acid-containing mucins by electron microscopy was investigated. Three kinds of mouse ascites tumor cells were fixed in neutral buffered formalin, exposed to fresh colloidal ferric oxide, treated with potassium ferrocyanide, imbedded in Selectron, and sectioned for electron microscopy. Additional staining with uranyl acetate and potassium permanganate was done after sectioning in order to increase contrast. Those cells known to be coated with sialomucin showed deposits of electron-opaque ferric ferrocyanide crystals in the areas where sialomucin concentrations were expected. When these cells were treated with neuraminidase beforehand, these deposits did not appear. It was concluded that, with the precautions and modifications described, the Hale stain can be successfully combined with electron microscopy to identify sialomucin.

Uranyl Acetate as a Fixative—from pH 2.0 to 8.0

1971 ◽  
Vol 29 ◽  
pp. 490-491
Author(s):  
V. R. Mumaw ◽  
B. L. Munger
Keyword(s):  
Electron Microscopy ◽  
Oxalic Acid ◽  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
En Bloc ◽  
Tissue Sections ◽  
Normal Rat ◽  
Lead Hydroxide ◽  
Ultrathin Sections ◽  
Rat Tissue

Numerous applications utilizing uranyl acetate as an electron stain for electron microscopy have been described. Uranyl acetate has become a routine stain used in conjunction with lead hydroxide for staining ultrathin sections. En bloc staining with uranyl acetate following osmium tetroxide post-fixation produces undesirable effects on some cytoplasmic components, especially glycogen. Recent studies using uranyl acetate as a fixative and en bloc stain at pH 7.2 before osmification has shown uranyl acetate to have desirable fixation and staining qualities. Tissues treated with uranyl acetate at a pH of 2.0-8.0 were studied. Normal rat tissue was fixed in Karnovsky's paraformaldehyde-glutaraldehyde fixative. The tissue was post-fixed in 0.5% uranyl acetate in water at pH 2.0 and 0.5% uranyl acetate in 0.1M s-collidine with 0.01M oxalic acid at pH 4, pH 6.0, pH 7.2, and pH 8.0 for 1 hour at 4°C. Following several rinses of 0.1M s-collidine buffer, the tissues were treated with 1.33% osmium tetroxide 1 hour at 4°C followed by rapid dehydration in ethanol and embedded in Durcupan ACM. Tissue sections were stained with lead hydroxide.

Organs of a Baikal seal affected by a morbillivirus

1990 ◽  
Vol 48 (3) ◽  
pp. 968-969
Author(s):  
O.I. Belykh ◽  
Ye.V. Likhoshway ◽  
Yu.V. Solodun ◽  
O.A. Goldberg ◽  
V.P. Kumarev
Keyword(s):  
Electron Microscopy ◽  
Measles Virus ◽  
Present Report ◽  
Protein A ◽  
Uranyl Acetate ◽  
Kidney Cells ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
Liver And Kidney ◽  
Ultrathin Sections

The population of Baikal seals Phoca sibirica has been plagued in 1987-88 by an unknown disease. Oligonucleotide probing of nucleic acids isolated from tissues of ill and dead animals, as well as immunological evidence and clinical data suggested that seals were infected by a morbillivirus. Morbillivirus antigen has been vizualized in dead seal tissues by immunoelectron microscopy (preembedding technique).The present report gives outline of electron microscopic studies of the tissues of infected Baikal seals. Morbillivirus antigens were vizualized as clusters of gold spheres by postembedding technique with monoclonal antibodies against measles virus and protein A-colloid gold conjugates in nuclei and cytoplasm of liver and kidney cells. Some clusters were associated with virus-like particles having a diameter of 80-100 nm. Electron microscopy of ultrathin sections stained with uranyl acetate revealed nucleocapsides having length of up to 1400 nm, and a diameter of 13-17 nm, morphologically similar to measles and seals distemper virus.

scholarly journals FINE STRUCTURE OBSERVATIONS OF THE UPTAKE OF INTRAVENOUSLY INJECTED PEROXIDASE BY THE RAT CHOROID PLEXUS

1967 ◽  
Vol 15 (3) ◽  
pp. 160-165 ◽  
Author(s):  
NORWIN H. BECKER ◽  
ALEX B. NOVIKOFF ◽  
H. M. ZIMMERMAN
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Cerebrospinal Fluid ◽  
Choroid Plexus ◽  
Smooth Endoplasmic Reticulum ◽  
Multivesicular Bodies ◽  
Adult Rats ◽  
Dense Bodies ◽  
Pinocytotic Vesicles ◽  
Membrane Bound

The uptake by the choroid plexus of adult rats of intravenously injected horseradish peroxidase has been investigated by electron microscopy. Within 4 min, the injected protein passes the capillary and is rapidly distributed through extracellular space and choroidal cells. Peroxidase enters the choroidal cells within coated vesicles which act as pinocytotic vesicles. At 15 min, peroxidase activity is present in numerous membrane-bound vesicles, multivesicular bodies, dense bodies and what appear to be segments of smooth endoplasmic reticulum. None of the peroxidase-containing organelles is seen to empty to the ventricular surface. Egress of the extracellular peroxidase into the cerebrospinal fluid is apparently blocked by apical zonulae occludentes between the choroidal cells.

The Use of Lead and Uranyl Ions As A Stain in Scanning Electron Microscopy

1976 ◽  
Vol 34 ◽  
pp. 314-315
Author(s):  
V. R. Mumaw ◽  
M. P. Goheen ◽  
B. L. Munger
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Uranyl Acetate ◽  
Distilled Water ◽  
Uranyl Ions ◽  
Cell Organelles ◽  
Lead Hydroxide ◽  
Definition Of ◽  
Cellular Components ◽  
Scanning Electron

Cellular components can be identified in cryofractured surfaces of osmium impregnated tissue examined in the scanning electron microscope (SEM). The present study compares the staining properties of uranyl and lead ions used to selectively enhance the definition of cell organelles. Tissues used for this study were liver, kidney and testis from normal rats. All of the tissue in this study was treated with osmium-thiocarbohydrazide-osmium (OTO) as described by Munger and Mumaw (1). Following the OTO treatment some tissues were placed in 1% aqueous uranyl acetate for 4 hrs. at room temp. Tissue stained with lead was placed in a Millonig's lead hydroxide solution for 2 hrs. Some tissue blocks were stained with uranyl acetate for 4 hrs. followed with 2 hrs. staining in lead hydroxide. Following each staining period the tissue was rinsed several times with distilled water before proceeding with the next step.

Mouse Hepatoblastomas: A Histologic, Ultrastructural, and Immunohistochemical Study

1988 ◽  
Vol 25 (4) ◽  
pp. 286-296 ◽  
Author(s):  
T. Nonoyama ◽  
F. Fullerton ◽  
G. Reznik ◽  
T. J. Bucci ◽  
J. M. Ward
Keyword(s):  
Electron Microscopy ◽  
Tumor Cells ◽  
Immunohistochemical Study ◽  
Light And Electron Microscopy ◽  
Alpha Fetoprotein ◽  
Cell Populations ◽  
Bile Canaliculi ◽  
Cytoplasmic Organelles ◽  
Dense Bodies ◽  
Hepatocellular Adenomas

Hepatoblastomas from B6C3F1 and BALB/c mice were examined by light and electron microscopy and by immunohistochemical reactions for alpha-fetoprotein, keratin, and vimentin. Tumors occurred in one group of a chronic bioassay for the interaction of diet, genetic strain, and the carcinogen, 2-acetylaminofluorene. Tumors had several populations (including epithelial and mesenchymal cells) in various stages of differentiation. Neoplastic epithelial cells had features of embryonal hepatocytes, such as sparse cytoplasmic organelles, absence of glycogen, abundant free ribosomes, occasional bile canaliculi, and peroxisome-like dense bodies. Embryonal fibroblast-like cells had pleomorphic and folded nuclei with prominent perinuclear chromatin and dispersed cytoplasmic organelles. Fibroblast-like cells were surrounded by bundles of collagen fibrils. Intermediate or transitional types of cells were seen. No tumor cells were immunoreactive for mouse alpha-fetoprotein (AFP) antibody, unlike those in hepatocellular adenomas or carcinomas. Epithelial and mesenchymal tumor cells contained intermediate filaments throughout the cytoplasm; some of these cells stained for keratin but not for vimentin. These findings suggest that mouse hepatoblastomas are derived from bipotential liver blastema cells and are composed of a mixture of several cell populations.

Immunogold localization of β1 integrin in mature avian smooth muscle

1995 ◽  
Vol 53 ◽  
pp. 1048-1049
Author(s):  
M. Stromer
Keyword(s):  
Electron Microscopy ◽  
Smooth Muscle ◽  
Cell Types ◽  
Immunogold Labeling ◽  
Β1 Integrin ◽  
Immunogold Localization ◽  
Binding Region ◽  
Dense Bodies ◽  
Transmission Electron ◽  
Membrane Bound

Integrins are α β heterodimeric transmembrane glycoproteins. The extracellular aminoterminal portion of the dimer contains the ligand-binding region and provides a linkage to the extracellular matrix and to other cells. The relatively smaller cytoplasmic carboxyterminal region interacts with the cytoskeleton and specifically interacts with α-actinin and with talin. Integrins are, thus, thought to be involved in signal transduction at the surface of many cell types. Smooth muscle cells contain α1 β1 integrin which has been immunolocalized in fibroblast focal contacts. Because aα-actinin and talin are components of attachment plaques which are sometimes referred to as plasma membrane-bound dense bodies in smooth muscle, we used immunogold labeling and transmission electron microscopy (TEM) to determine the location of β1 integrin at the smooth muscle cell surface.Strips of smooth muscle from mature chicken gizzards were isometrically clamped and were fixed in 2% paraformaldehyde in modified Krebs Ringer that contained 2mM Ca2+ and 2mM Mg2+ for 2½ hrs. at 2° C. Fixed strips were cut into 0.5-0.8mm cubes that were immersed in 0.15 M glycine for 1 hr.

Platinum Compounds as Stains for Electron Microscopy

1974 ◽  
Vol 32 ◽  
pp. 230-231
Author(s):  
S. K. Aggarwal ◽  
P. McAllister ◽  
R. W. Wagner ◽  
B. Rosenberg
Keyword(s):  
Electron Microscopy ◽  
Hela Cells ◽  
Uranyl Acetate ◽  
Sarcoma 180 ◽  
Platinum Compounds ◽  
Kb Cells ◽  
En Bloc ◽  
Human Lymphoma ◽  
Ultrathin Sections ◽  
Blue Coloration

Uranyl acetate has been used as an electron stain for en bloc staining as well as for staining ultrathin sections in conjunction with various lead stains (Fig. 1). Present studies reveal that various platinum compounds also show promise as electron stains. Certain platinum compounds have been shown to be effective anti-tumor agents. Of particular interest are the compounds with either uracil or thymine as one of the ligands (cis-Pt(II)-uracil; cis-Pt(II)-thymine). These compounds are amorphous, highly soluble in water and often exhibit an intense blue coloration. These compounds show enough electron density to be used as stains for electron microscopy. Most of the studies are based on various cell lines (human AV, cells, human lymphoma cells, KB cells, Sarcoma-180 ascites cells, chick fibroblasts and HeLa cells) while studies on tissue blocks are in progress.

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