Effect of Substrate on Indirect Immunofluorescence Test for Canine Pemphigus Foliaceus

The effect of substrate on indirect immunofluorescence (IIF) tests for the detection of circulating autoantibodies was studied by examining sera from 14 canine pemphigus foliaceus patients, six sera with non-pemphigus dermatoses and ten normal dog sera against five different substrates from three species. These substrates included bovine esophagus, bovine nose, bovine tongue, monkey esophagus, and canine nose skin. Nine out of 14 (64.3%) sera from patients with canine pemphigus foliaceus showed intercellular space staining by indirect immunofluorescence using bovine esophagus as substrate. However, sera from nonpemphigus dermatoses and normal dog did not react with bovine esophagus. In other substrates, only bovine tongue showed 1/8 (12.5%) positive reaction at the intercellular space by sera from canine pemphigus foliaceus. Dog nose skin showed the intercellular space staining against ten of ten (100%) normal dog serum. Monkey esophagus showed the fluorescent deposit at the intercellular space in four of nine (44.4%) of pemphigus foliacues dog sera, however, four of ten (40%) of normal dog sera revealed nonspecific intercellular staining. These results indicate that the sensitivity and the specifity of IIF test in canine pemphigus foliaceus depend on the substrate. The best substrate for detecting circulating autoantibody in canine pemphigus foliaceus patients among five different substrates was bovine esophagus because of its sensitivy and high specifity. The diagnosis of canine pemphigus foliaceus should be made on the basis of a combination of clinical signs, histopathology, direct immunofluorescence, and the detection of circulating autoantibody.

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Cell and humoral immunity in endemic pemphigus foliaceus

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46651995000100005 ◽

1995 ◽

Vol 37 (1) ◽

pp. 25-34

Author(s):

Silvia Regina C. Sartori Barravieira ◽

Neuza Lima Dillon ◽

Maria Tereza Rezkallah-Iwasso ◽

Maria Terezinha Serrão Peraçoli ◽

Paulo Roberto Curi ◽

...

Keyword(s):

Indirect Immunofluorescence ◽

Laboratory Data ◽

Clinical Signs ◽

Lymphocyte Transformation ◽

Skin Lesions ◽

Direct Immunofluorescence ◽

Pemphigus Foliaceus ◽

Immunofluorescence Test ◽

Indirect Immunofluorescence Test ◽

Cell Immunity

A study was conducted on 16 patients with pemphigus foliaceus, ten of them with the localized form (group G1) and six with the disseminated form (group G2). These patients were submitted to full blood counts, quantitation of mononuclear cell subpopulations by monoclonal antibodies, study of blastic lymphocyte transformation, and quantitation of circulating antibodies by the indirect immunofluorescence test, in order to correlate their clinical signs and symptoms and laboratory data with their immunological profile, and to determine the relationship between circulating autoantibody titers and lesion intensity and course of lesions under treatment. Leucocytosis was observed especially in group G2. All patients showed decreased relative CD3+ and CD4+ values and a tendency to decreased relative values of the CD8+ subpopulation. Blastic lymphocyte transformation indices in the presence of phytohemagglutinin were higher in patients (group G1+G2) than in controls. The indirect immunofluorescence test was positive in 100% of G2 patients and in 80% of G1 patients. The median value for the titers was higher in group G2 than in group G1. Analysis of the results as a whole permits us to conclude that cell immunity was preserved and that there was a relationship between antibody titers detected by the direct immunofluorescence test and extent of skin lesions.

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Indirect immunofluorescence test for serodiagnosis of Legionnaires disease: evidence for serogroup diversity of Legionnaires disease bacterial antigens and for multiple specificity of human antibodies

Journal of Clinical Microbiology ◽

10.1128/jcm.9.3.379-383.1979 ◽

1979 ◽

Vol 9 (3) ◽

pp. 379-383

Author(s):

H W Wilkinson ◽

B J Fikes ◽

D D Cruce

Keyword(s):

Indirect Immunofluorescence ◽

Direct Immunofluorescence ◽

Immunofluorescence Test ◽

Serological Tests ◽

Fourfold Increase ◽

Human Antibodies ◽

Indirect Immunofluorescence Test ◽

Human Sera ◽

Legionnaires Disease ◽

Group 2

Evidence obtained by others who used direct immunofluorescence staining to demonstrate serological differences among strains of Legionnaires disease bacterium prompted this study of parameters influencing the ability of the indirect immunofluorescence test to detect human antibodies to Legionnaires disease bacterium. A total of 25 Legionnaires disease bacterium strains, representing four serogroups, were used as immunofluorescence antigens to test selected human sera. The use of diethyl ether in preparing the antigens was discontinued when it was found that titers against ether-killed group 2 (Togus 1-like) antigens were impossible to determine. Instead, heat-killed suspensions of Legionnaires disease bacterium in 0.5% buffered normal chicken yolk sac were used to show the serogroup diversity of the strains and the serogroup specificity of the antibody response of some, but not all, patients with serological evidence of Legionnaires disease. These studies suggest that multiple antigens should be used in serological tests for Legionnaires disease. Furthermore, the fact that some sera contain antibodies that bind equally well to strains of all four serogroups implies that demonstration of a fourfold increase in titer of paired sera when tested with a single antigen should not be interpreted as evidence of infection with a strain of the same serogroup.

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An Erythrodermic Variant of Pemphigus Foliaceus with Puzzling Histologic and Immunopathologic Features

Journal of Cutaneous Medicine and Surgery ◽

10.2310/7750.2007.00021 ◽

2007 ◽

Vol 11 (5) ◽

pp. 179-184 ◽

Cited By ~ 5

Author(s):

Jennifer D. Peterson ◽

Sophie M. Worobec ◽

Lawrence S. Chan

Keyword(s):

Indirect Immunofluorescence ◽

Antinuclear Antibody ◽

Granular Layer ◽

Basement Membrane Zone ◽

Direct Immunofluorescence ◽

Pemphigus Foliaceus ◽

Laboratory Studies ◽

Drug Induced ◽

Neutrophilic Infiltrate ◽

Monkey Esophagus

Background: Pemphigus foliaceus is an autoimmune blistering disorder that affects the skin owing to autoantibodies against desmoglein 1. Methods: We employed clinical, histologic, immunopathologic, and serum laboratory studies to investigate a case of an erythrodermic variant of pemphigus foliaceus in an elderly man following treatment with bisoprolol-hydrochlorothiazide. Results: Early histopathology revealed psoriasiform dermatitis, but later biopsies showed subcorneal and granular layer separation with neutrophilic infiltrate. Direct immunofluorescence showed intercellular deposits of immunoglobulin G throughout the epidermis, granular staining of C3 along the basement membrane zone, and fibrin and C3 deposition around the blood vessels. Indirect immunofluorescence on monkey esophagus showed a titer of greater than 1:1,280. Indirect immunofluorescence on rat bladder, antinuclear antibody, lupus panel, and kidney function panel were all negative. Conclusion: There are no reports in the literature of pemphigus foliaceus being induced by bisoprolol, but reports exist of propanolol resulting in drug-induced pemphigus foliaceus.

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Recognition of a Non-HLA-ABC Antigen Present on B and T Lymphocytes and Monocytes only Detectable with the Indirect Immunofluorescence Test

Vox Sanguinis ◽

10.1159/000460447 ◽

1979 ◽

Vol 36 (3) ◽

pp. 150-158

Author(s):

F. Décary ◽

F.W.A. Verheugt ◽

L. van Helden-Henningheim ◽

E. von Riesz ◽

R. Schreuder-van Gelder ◽

...

Keyword(s):

T Lymphocytes ◽

Indirect Immunofluorescence ◽

Immunofluorescence Test ◽

Indirect Immunofluorescence Test ◽

Antigen Present ◽

B And T Lymphocytes

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Behaviour of indirect immunofluorescence test and some cerebrospinal parameters in neurocysticercosis

Arquivos de Neuro-Psiquiatria ◽

10.1590/s0004-282x1987000100005 ◽

1987 ◽

Vol 45 (1) ◽

pp. 33-43 ◽

Cited By ~ 2

Author(s):

Amauri Braga Simonetti ◽

Jorge Teixeira

Keyword(s):

Cerebrospinal Fluid ◽

Indirect Immunofluorescence ◽

False Positive ◽

Clinical Evidence ◽

Routine Analysis ◽

Immunofluorescence Test ◽

Indirect Immunofluorescence Test ◽

Immunofluorescent Test ◽

Positive Results

Cerebrospinal fluid from 53 patients with clinical evidence of neurocysticercosis and 11 who suffered from several diseases were studied to evaluate the behaviour of indirect immunofluorescence test and some parameters of routine analysis. In neurocysticercosis there were pleocitosis in 88.7% of cases, eosinophilorrachia in 60.3%o, hyperproteinorrachia in 71.7% and hypoglucorrachia in 13.2%. The indirect immunofluorescence test was positive in 19.2% of cases but false-positive results were found when the samples showed xanthochromia or erythrocyte contamination. The authors discuss their results in comparison with those in literature and conclude that the immunofluorescent test is sensitive and useful in diagnosis of neurocysticercosis, except when the interferents previously mentioned are present.

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