scholarly journals Cell and humoral immunity in endemic pemphigus foliaceus

1995 ◽  
Vol 37 (1) ◽  
pp. 25-34
Author(s):  
Silvia Regina C. Sartori Barravieira ◽  
Neuza Lima Dillon ◽  
Maria Tereza Rezkallah-Iwasso ◽  
Maria Terezinha Serrão Peraçoli ◽  
Paulo Roberto Curi ◽  
...  
Keyword(s):  
Indirect Immunofluorescence ◽  
Laboratory Data ◽  
Clinical Signs ◽  
Lymphocyte Transformation ◽  
Skin Lesions ◽  
Direct Immunofluorescence ◽  
Pemphigus Foliaceus ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Cell Immunity

A study was conducted on 16 patients with pemphigus foliaceus, ten of them with the localized form (group G1) and six with the disseminated form (group G2). These patients were submitted to full blood counts, quantitation of mononuclear cell subpopulations by monoclonal antibodies, study of blastic lymphocyte transformation, and quantitation of circulating antibodies by the indirect immunofluorescence test, in order to correlate their clinical signs and symptoms and laboratory data with their immunological profile, and to determine the relationship between circulating autoantibody titers and lesion intensity and course of lesions under treatment. Leucocytosis was observed especially in group G2. All patients showed decreased relative CD3+ and CD4+ values and a tendency to decreased relative values of the CD8+ subpopulation. Blastic lymphocyte transformation indices in the presence of phytohemagglutinin were higher in patients (group G1+G2) than in controls. The indirect immunofluorescence test was positive in 100% of G2 patients and in 80% of G1 patients. The median value for the titers was higher in group G2 than in group G1. Analysis of the results as a whole permits us to conclude that cell immunity was preserved and that there was a relationship between antibody titers detected by the direct immunofluorescence test and extent of skin lesions.

Effect of Substrate on Indirect Immunofluorescence Test for Canine Pemphigus Foliaceus

1996 ◽  
Vol 33 (3) ◽  
pp. 332-336 ◽  
Author(s):  
T. Iwasaki ◽  
M. Shimizu ◽  
H. Obata ◽  
M. Ogata ◽  
M. Nagata ◽  
...  
Keyword(s):  
Indirect Immunofluorescence ◽  
Intercellular Space ◽  
Clinical Signs ◽  
Direct Immunofluorescence ◽  
Pemphigus Foliaceus ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
High Specifity ◽  
Circulating Autoantibodies ◽  
Monkey Esophagus

The effect of substrate on indirect immunofluorescence (IIF) tests for the detection of circulating autoantibodies was studied by examining sera from 14 canine pemphigus foliaceus patients, six sera with non-pemphigus dermatoses and ten normal dog sera against five different substrates from three species. These substrates included bovine esophagus, bovine nose, bovine tongue, monkey esophagus, and canine nose skin. Nine out of 14 (64.3%) sera from patients with canine pemphigus foliaceus showed intercellular space staining by indirect immunofluorescence using bovine esophagus as substrate. However, sera from nonpemphigus dermatoses and normal dog did not react with bovine esophagus. In other substrates, only bovine tongue showed 1/8 (12.5%) positive reaction at the intercellular space by sera from canine pemphigus foliaceus. Dog nose skin showed the intercellular space staining against ten of ten (100%) normal dog serum. Monkey esophagus showed the fluorescent deposit at the intercellular space in four of nine (44.4%) of pemphigus foliacues dog sera, however, four of ten (40%) of normal dog sera revealed nonspecific intercellular staining. These results indicate that the sensitivity and the specifity of IIF test in canine pemphigus foliaceus depend on the substrate. The best substrate for detecting circulating autoantibody in canine pemphigus foliaceus patients among five different substrates was bovine esophagus because of its sensitivy and high specifity. The diagnosis of canine pemphigus foliaceus should be made on the basis of a combination of clinical signs, histopathology, direct immunofluorescence, and the detection of circulating autoantibody.

scholarly journals Indirect immunofluorescence test for serodiagnosis of Legionnaires disease: evidence for serogroup diversity of Legionnaires disease bacterial antigens and for multiple specificity of human antibodies

1979 ◽  
Vol 9 (3) ◽  
pp. 379-383
Author(s):  
H W Wilkinson ◽  
B J Fikes ◽  
D D Cruce
Keyword(s):  
Indirect Immunofluorescence ◽  
Direct Immunofluorescence ◽  
Immunofluorescence Test ◽  
Serological Tests ◽  
Fourfold Increase ◽  
Human Antibodies ◽  
Indirect Immunofluorescence Test ◽  
Human Sera ◽  
Legionnaires Disease ◽  
Group 2

Evidence obtained by others who used direct immunofluorescence staining to demonstrate serological differences among strains of Legionnaires disease bacterium prompted this study of parameters influencing the ability of the indirect immunofluorescence test to detect human antibodies to Legionnaires disease bacterium. A total of 25 Legionnaires disease bacterium strains, representing four serogroups, were used as immunofluorescence antigens to test selected human sera. The use of diethyl ether in preparing the antigens was discontinued when it was found that titers against ether-killed group 2 (Togus 1-like) antigens were impossible to determine. Instead, heat-killed suspensions of Legionnaires disease bacterium in 0.5% buffered normal chicken yolk sac were used to show the serogroup diversity of the strains and the serogroup specificity of the antibody response of some, but not all, patients with serological evidence of Legionnaires disease. These studies suggest that multiple antigens should be used in serological tests for Legionnaires disease. Furthermore, the fact that some sera contain antibodies that bind equally well to strains of all four serogroups implies that demonstration of a fourfold increase in titer of paired sera when tested with a single antigen should not be interpreted as evidence of infection with a strain of the same serogroup.

Recognition of a Non-HLA-ABC Antigen Present on B and T Lymphocytes and Monocytes only Detectable with the Indirect Immunofluorescence Test

Vox Sanguinis ◽  
1979 ◽  
Vol 36 (3) ◽  
pp. 150-158
Author(s):  
F. Décary ◽  
F.W.A. Verheugt ◽  
L. van Helden-Henningheim ◽  
E. von Riesz ◽  
R. Schreuder-van Gelder ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Indirect Immunofluorescence ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Antigen Present ◽  
B And T Lymphocytes

scholarly journals Behaviour of indirect immunofluorescence test and some cerebrospinal parameters in neurocysticercosis

1987 ◽  
Vol 45 (1) ◽  
pp. 33-43 ◽  
Author(s):  
Amauri Braga Simonetti ◽  
Jorge Teixeira
Keyword(s):  
Cerebrospinal Fluid ◽  
Indirect Immunofluorescence ◽  
False Positive ◽  
Clinical Evidence ◽  
Routine Analysis ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Immunofluorescent Test ◽  
Positive Results

Cerebrospinal fluid from 53 patients with clinical evidence of neurocysticercosis and 11 who suffered from several diseases were studied to evaluate the behaviour of indirect immunofluorescence test and some parameters of routine analysis. In neurocysticercosis there were pleocitosis in 88.7% of cases, eosinophilorrachia in 60.3%o, hyperproteinorrachia in 71.7% and hypoglucorrachia in 13.2%. The indirect immunofluorescence test was positive in 19.2% of cases but false-positive results were found when the samples showed xanthochromia or erythrocyte contamination. The authors discuss their results in comparison with those in literature and conclude that the immunofluorescent test is sensitive and useful in diagnosis of neurocysticercosis, except when the interferents previously mentioned are present.

Evaluation of the automated indirect immunofluorescence test for anti-dsDNA antibodies

2019 ◽  
Vol 498 ◽  
pp. 143-147 ◽  
Author(s):  
Shiji Wu ◽  
Feng Wang ◽  
Jin Huang ◽  
Botao Yin ◽  
Min Huang ◽  
...  
Keyword(s):  
Indirect Immunofluorescence ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Dsdna Antibodies

Correlation between enzyme immunoassay and indirect immunofluorescence test for high titer of anti-ribosomal-P antibodies

2012 ◽  
Vol 413 (5-6) ◽  
pp. 650-652 ◽  
Author(s):  
Kyeong-Hee Kim ◽  
Bo-Ram Kim ◽  
Ji-Eun Kim ◽  
Kwang-Sook Woo ◽  
Jin-Yeong Han ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Indirect Immunofluorescence ◽  
High Titer ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Ribosomal P

Therapy of Alveococcosis in Man

1977 ◽  
Vol 5 (5) ◽  
pp. 367-368 ◽  
Author(s):  
C E Lüdin ◽  
K Gyr ◽  
K Karoussos
Keyword(s):  
Partial Hepatectomy ◽  
Indirect Immunofluorescence ◽  
The Other ◽  
Immunofluorescence Test ◽  
Serological Tests ◽  
Passive Haemagglutination ◽  
Indirect Immunofluorescence Test ◽  
Reliable Methods

Diagnostic, therapeutic and prognostic aspects are interpreted on the basis of two cases of Echinococcus alveolaris. Both patients had undergone partial hepatectomy and were subsequently treated chemotherapeutically. In one case dehydroemetine and mebendazole (Vermox®, Janssen), and in the other case metrifonate (Bilarcil®, Bayer A. G.) were used. The diagnosis of alveococcosis of the liver was demonstrated in one case histologically on the occasion of appendectomy, and in the other case by the indirect immunofluorescence test and passive haemagglutination. In case of suspected alveococcosis these serological tests are imperative, as they are the most reliable methods of demonstrating this disease. The latest trend in therapy aims at a combination of surgery and chemotherapy. Mebendazole seems to be promising as an anthelmintic agent for the treatment of alveococcosis. Remission of the disease was obtained in either case.

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