Interleukin-1 excretion in urine specimens of renal transplant recipients with acute rejection using matrix dot-blot assay

1996 ◽  
Vol 63 (4) ◽  
pp. 421-423
Author(s):  
A. Mukhtar
Keyword(s):  
Renal Transplant ◽  
Acute Rejection ◽  
Inflammatory Responses ◽  
Mesangial Cells ◽  
Interleukin 1 ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Dot Blot ◽  
Dot Blot Assay ◽  
Urine Specimens

Interleukin-1 is a polypeptide mediator involved in the regulation of inflammatory responses. Mesangial cells IL-1 may act as an autocrine growth factor (Lovett et al., 1983). The local release of such growth factors may be important in the development of mesangial proliferative lesions characteristic of many forms of immune-mediated glomerulonephritis (Lovett et al., 1986). Secretion of IL-1 in urine specimens of renal transplant recipients with acute rejection was studied using matrix APAAP dot-blot assay, to determine any alteration of IL-1 changes that could reveal any pathological correlation. Twelve urine specimens from 6 persons with acute rejection revealed IL-1 staining in the dot-blot assay while there was no staining for IL-1 in the urine specimens of healthy persons. The immunological changes correlated with acute renal rejection could lead to autocrine and apocrine secretion of IL-1 its secretion in urine.

Effect of Lyophilized Urine of Acute Rejected Kidney Transplant Recipients on Mp48 Production by Mesangial Cells in Culture

1996 ◽  
Vol 63 (2) ◽  
pp. 258-260
Author(s):  
A. Mukhtar
Keyword(s):  
Renal Transplant ◽  
Mesangial Cells ◽  
Renal Transplant Recipients ◽  
Normal Kidney ◽  
Transplant Recipients ◽  
Kidney Transplant Recipients ◽  
Liver And Pancreas ◽  
Immunological Changes ◽  
Urine Specimens ◽  
Healthy Persons

MP48 is a protein expressed only in stimulated mesangials cells. This protein is expressed in diseased but not in normal kidney sections. It is also expressed in the liver and pancreas but not in the erythrocytes of healthy persons, whereas it is expressed in the erythrocytes of renal transplant recipients. This is the first study of the effect of lyophilized urine on MP48. The study showed that urine samples of acute rejected renal transplant recipients stimulated the cultured mesangial cells to produce MP48 protein, while the urine specimens of twelve normal persons did not. The immunological changes correlated with the acute renal rejection could lead to autocrine and apocrine secretion of IL-1 and other growth factors and their secretion in urine. Therefore, this experiment could be used to detect the effect of lyophilized urine specimens of acute rejected renal transplant recipients on cultured mesangial cells for production of MP48.

NFATC1genotypes affect acute rejection and long-term graft function in cyclosporine-treated renal transplant recipients

2017 ◽  
Vol 18 (4) ◽  
pp. 381-392 ◽  
Author(s):  
Qinxia Xu ◽  
Xiaoyan Qiu ◽  
Zheng Jiao ◽  
Ming Zhang ◽  
Jianping Chen ◽  
...  
Keyword(s):  
Renal Transplant ◽  
Acute Rejection ◽  
Graft Function ◽  
Renal Transplant Recipients ◽  
Transplant Recipients

Diagnostic significance of some urinary enzymes for detecting acute rejection crises in renal-transplant recipients: alanine aminopeptidase, alkaline phosphatase, gamma-glutamyltransferase, N-acetyl-beta-D-glucosaminidase, and lysozyme.

1986 ◽  
Vol 32 (10) ◽  
pp. 1807-1811 ◽  
Author(s):  
K Jung ◽  
J Diego ◽  
V Strobelt ◽  
D Scholz ◽  
G Schreiber
Keyword(s):  
Alkaline Phosphatase ◽  
Renal Transplant ◽  
Acute Rejection ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Urinary Enzymes ◽  
Gamma Glutamyltransferase ◽  
Alanine Aminopeptidase ◽  
Group A ◽  
Group B

Abstract We compared the diagnostic validity of five urinary enzymes--alanine aminopeptidase (EC 3.4.11.2), alkaline phosphatase (EC 3.1.3.1), gamma-glutamyltransferase (EC 2.3.2.2), N-acetyl-beta-D-glucosaminidase (EC 3.2.1.30), and lysozyme (EC 3.2.1.17)--as indicators of acute rejection crises in renal-transplant recipients. In 82 patients (group A), the excretion of each of these five enzymes was measured daily from transplantation until discharge from hospital. In another 69 patients (group B), enzyme determinations were made when the patient came for regular checkups (about every four to eight weeks). We used an "activity ratio" (the activity measured at a particular time compared with the activity on the preceding determination) value of 1.5 as the decision point. In group A, use of this discrimination point for alanine aminopeptidase, gamma-glutamyltransferase, and N-acetyl-beta-D-glucosaminidase yielded a specificity and sensitivity of about 90%. In group B, only alanine aminopeptidase had a greater diagnostic sensitivity than creatinine alone. Evidently, measurement of alanine aminopeptidase can be a helpful indicator of acute rejection crises, when interpreted in combination with other available relevant clinical, biochemical, and immunological data.

Sign in / Sign up

Export Citation Format

Share Document