scholarly journals Porcine Neural Progenitor Cells Derived from Tissue at Different Gestational Ages Can Be Distinguished by Global Transcriptome

2017 ◽  
Vol 26 (9) ◽  
pp. 1582-1595
Author(s):  
Jing Yang ◽  
Steven Menges ◽  
Ping Gu ◽  
Ronald Tongbai ◽  
Melissa Samuel ◽  
...  
Keyword(s):  
Gene Expression ◽  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Expression Profiles ◽  
Quantitative Polymerase Chain Reaction ◽  
Neural Progenitor ◽  
Age Related ◽  
Donor Cells ◽  
Global Transcriptome ◽  
The Impact

The impact of gestational age on mammalian neural progenitor cells is potentially important for both an understanding of neural development and the selection of donor cells for novel cell-based treatment strategies. In terms of the latter, it can be problematic to rely entirely on rodent models in which the gestational period is significantly shorter and the brain much smaller than is the case in humans. Here, we analyzed pig brain progenitor cells (pBPCs) harvested at 2 different gestational ages (E45 and E60) using gene expression profiles, obtained by microarray analysis and quantitative polymerase chain reaction (qPCR), across time in culture. Comparison of the global transcriptome of pBPCs from age-matched transgenic green flourescent protein (GFP)-expressing fetuses versus non- GFP-expressing fetuses did not reveal significant differences between the 2 cell types, whereas comparison between E45 and E60 pBPCs did show separation between the data sets by principle component analysis. Further examination by qPCR showed evidence of relative downregulation of proliferation markers and upregulation of glial markers in the gestationally older (E60) cells. Additional comparisons were made. This study provides evidence of age-related changes in the gene expression of cultured fetal porcine neural progenitors that are potentially relevant to the role of these cells during development and as donor cells for transplantation studies.

scholarly journals Downregulation of Neurodevelopmental Gene Expression in iPSC-Derived Cerebral Organoids Upon Infection by Human Cytomegalovirus

2021 ◽  
Author(s):  
Benjamin Shea O'Brien ◽  
Rebekah L Mokry ◽  
Megan L Schumacher ◽  
Kirthi Pulakanti ◽  
Sridhar Rao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Progenitor Cells ◽  
Human Cytomegalovirus ◽  
Neural Progenitor Cells ◽  
Treatment Options ◽  
Congenital Infection ◽  
Induced Pluripotent Stem Cell ◽  
Neural Progenitor ◽  
Human Neurons ◽  
The Impact

Human cytomegalovirus (HCMV) is a beta herpesvirus that, upon congenital infection, can cause severe birth defects including vision and hearing loss, microcephaly, and seizures. Currently, no approved treatment options exist for in utero infections. We previously demonstrated that HCMV infection decreases calcium signaling responses and alters neuronal differentiation in induced pluripotent stem cell (iPSC) derived neural progenitor cells (NPCs). Here we aimed to determine the impact of infection on the transcriptome in developing human neurons using iPSC-derived 3-dimensional cerebral organoids. We infected iPSC-derived cerebral organoids with HCMV encoding eGFP and sorted cell populations based on GFP signal strength. Significant transcriptional downregulation was observed including in key neurodevelopmental gene pathways in both the GFP (+) and intermediate groups. Interestingly, the GFP (-) group also showed downregulation of the same targets indicating a mismatch between GFP expression and viral infection. Using a modified HCMV virus destabilizing IE 1 and 2 proteins, we still observed significant downregulation of neurodevelopmental gene expression in infected neural progenitor cells. Together, these data indicate that IE viral proteins are not the main drivers of neurodevelopmental gene dysregulation in HCMV infected neural tissues suggesting therapeutically targeting IE gene expression is insufficient to restore neural differentiation and function.

Gene Expression Profiles and Metabolic Changes in Embryonic Neural Progenitor Cells Under Low Oxygen

2011 ◽  
Vol 13 (2) ◽  
pp. 113-120 ◽  
Author(s):  
Ling-Ling Zhu ◽  
Tong Zhao ◽  
Xin huang ◽  
Zhao-hui Liu ◽  
Li-ying Wu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Neural Progenitor ◽  
Metabolic Changes ◽  
Low Oxygen

scholarly journals Xenotransplantation of Human Neural Progenitor Cells to the Subretinal Space of Nonimmunosuppressed Pigs

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Karin Warfvinge ◽  
Philip H. Schwartz ◽  
Jens Folke Kiilgaard ◽  
Morten la Cour ◽  
Michael J. Young ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Mononuclear Cell ◽  
Neural Progenitor Cells ◽  
Retinal Vasculitis ◽  
Donor Cell ◽  
Neural Progenitor ◽  
Morphological Integration ◽  
Subretinal Space ◽  
Donor Cells ◽  
Human Neural Progenitor Cells

To investigate the feasibility of transplanting human neural progenitor cells (hNPCs) to the retina of nonimmunosuppressed pigs, cultured hNPCs were injected into the subretinal space of 5 adult pigs after laser burns were applied to promote donor cell integration. Postoperatively, the retinal vessels appeared normal without signs of exudation, bleeding, or subretinal elevation. Eyes were harvested at 10–28 days. H&E consistently showed mild retinal vasculitis, depigmentation of the RPE, and marked mononuclear cell infiltrate in the choroid adjacent to the site of transplantation. Human-specific antibodies revealed donor cells in the subretinal space at 10–13 days and smaller numbers within the retina on days 12 and 13, with evidence suggesting a limited degree of morphological integration; however, no cells remained at 4 weeks. The strong mononuclear cell reaction and loss of donor cells indicate that modulation of host immunity is likely necessary for prolonged xenograft survival in this model.

Proliferation deficits and gene expression dysregulation in Down's syndrome (Ts1Cje) neural progenitor cells cultured from neurospheres

2009 ◽  
Vol 87 (14) ◽  
pp. 3143-3152 ◽  
Author(s):  
Randal X. Moldrich ◽  
Luce Dauphinot ◽  
Julien Laffaire ◽  
Tania Vitalis ◽  
Yann Hérault ◽  
...  
Keyword(s):  
Gene Expression ◽  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Down's Syndrome ◽  
Down’S Syndrome ◽  
Neural Progenitor ◽  
Gene Expression Dysregulation ◽  
Cells Cultured

scholarly journals Transduction of human neural progenitor cells with foamy virus vectors for differentiation-dependent gene expression

Gene Therapy ◽  
2008 ◽  
Vol 16 (3) ◽  
pp. 349-358 ◽  
Author(s):  
I Rothenaigner ◽  
S Kramer ◽  
M Meggendorfer ◽  
A Rethwilm ◽  
R Brack-Werner
Keyword(s):  
Gene Expression ◽  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Foamy Virus ◽  
Neural Progenitor ◽  
Virus Vectors ◽  
Human Neural Progenitor Cells

scholarly journals Store-Operated CRAC Channels Regulate Gene Expression and Proliferation in Neural Progenitor Cells

2014 ◽  
Vol 34 (27) ◽  
pp. 9107-9123 ◽  
Author(s):  
A. Somasundaram ◽  
A. K. Shum ◽  
H. J. McBride ◽  
J. A. Kessler ◽  
S. Feske ◽  
...  
Keyword(s):  
Gene Expression ◽  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Neural Progenitor ◽  
Regulate Gene Expression ◽  
Crac Channels ◽  
Regulate Gene
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