scholarly journals Isolation and molecular characterization of a urease-negative Actinobacillus pleuropneumoniae mutant

2017 ◽  
Vol 30 (1) ◽  
pp. 172-174 ◽  
Author(s):  
Hiroya Ito ◽  
Sayaka Takahashi ◽  
Tetsuo Asai ◽  
Yutaka Tamura ◽  
Koshi Yamamoto

An atypical urease-negative mutant of Actinobacillus pleuropneumoniae serovar 2 was isolated in Japan. Nucleotide sequence analysis of the urease gene cluster revealed that the insertion of a short DNA sequence into the cbiM gene was responsible for the urease-negative activity of the mutant. Veterinary diagnostic laboratories should be watchful for the presence of aberrant urease-negative A. pleuropneumoniae isolates.

2002 ◽  
Vol 68 (5) ◽  
pp. 2567-2571 ◽  
Author(s):  
Julianne Dyble ◽  
Hans W. Paerl ◽  
Brett A. Neilan

ABSTRACT Isolates of the toxic, N2-fixing species Cylindrospermopsis raciborskii from various geographic locations were analyzed with respect to their genetic diversity based on the nifH and cpcBA-IGS genes. Gene sequences clustered according to their geographic origin, with the nifH sequences separating into European, Australian, and American groups and the cpcBA-IGS sequences separating into American and European or Australian groups. PCR primers for both genes were designed to exclusively amplify DNA from Cylindrospermopsis species, and an additional primer set for cpcBA-IGS was designed to specifically amplify the American C. raciborskii strains.


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