scholarly journals Genetic Characterization of Cylindrospermopsis raciborskii (Cyanobacteria) Isolates from Diverse Geographic Origins Based on nifH and cpcBA-IGS Nucleotide Sequence Analysis

2002 ◽  
Vol 68 (5) ◽  
pp. 2567-2571 ◽  
Author(s):  
Julianne Dyble ◽  
Hans W. Paerl ◽  
Brett A. Neilan
Keyword(s):  
Genetic Diversity ◽  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Genetic Characterization ◽  
Geographic Origin ◽  
Pcr Primers ◽  
Cylindrospermopsis Raciborskii ◽  
Nucleotide Sequence Analysis ◽  
Geographic Origins

ABSTRACT Isolates of the toxic, N2-fixing species Cylindrospermopsis raciborskii from various geographic locations were analyzed with respect to their genetic diversity based on the nifH and cpcBA-IGS genes. Gene sequences clustered according to their geographic origin, with the nifH sequences separating into European, Australian, and American groups and the cpcBA-IGS sequences separating into American and European or Australian groups. PCR primers for both genes were designed to exclusively amplify DNA from Cylindrospermopsis species, and an additional primer set for cpcBA-IGS was designed to specifically amplify the American C. raciborskii strains.

Genetic Characterization of the Nitrate Reducing Community Based on narG Nucleotide Sequence Analysis

2003 ◽  
Vol 46 (1) ◽  
pp. 113-121 ◽  
Author(s):  
D. Ch�neby ◽  
S. Hallet ◽  
M. Mondon ◽  
F. Martin-Laurent ◽  
J. C. Germon ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Genetic Characterization ◽  
Nucleotide Sequence Analysis ◽  
Community Based

scholarly journals GENETIC DIFFERENTIATION OF THE KAMPAR RIVER'S GIANT FEATHERBACK (Chitala lopis BLEEKER 1851) BASE ON MITOCHONDRIAL DNA ANALYSIS

2017 ◽  
Vol 16 (2) ◽  
pp. 49 ◽  
Author(s):  
Arif Wibowo ◽  
Ridwan Affandi ◽  
Kadarwan Soewardi ◽  
Sudarto Sudarto
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Population Structure ◽  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Dna Analysis ◽  
Nucleotide Sequence Analysis ◽  
Mitochondrial Dna Control Region ◽  
Mitochondrial Dna Analysis ◽  
Important Fish

Although the giant featherback Chitala lopis is an important fish in Kampar River, the population structure has not been investigated. In this study, genetic diversity and population structure of giant featherback were examined using nucleotide sequence analysis of mitochondrial DNA control region for 54 fish collected from Kampar River.

scholarly journals Genetic diversity of the pandemic influenza A (H1N1) virus in Saudi Arabia

2014 ◽  
Vol 8 (12) ◽  
pp. 1563-1573 ◽  
Author(s):  
Norah Abdulhamed Uthman ◽  
Sayed Sartaj Sohrab ◽  
Ibrahim Hassan Kamal ◽  
Suha Abdulall Farraj ◽  
Badr Essa Masri ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Saudi Arabia ◽  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Pandemic Influenza ◽  
Influenza A ◽  
H1n1 Virus ◽  
Nucleotide Sequence Analysis ◽  
Influenza A H1n1 ◽  
Viral Diagnosis

Introduction: Pandemic influenza A (H1N1) virus emerged and spread globally in the spring of 2009. Saudi Arabia also witnessed a severe H1N1 pandemic virus epidemic with considerable morbidity and mortality in different parts of the kingdom beginning in June 2009. The influenza A(H1N1)pdm09 virus was detected in samples collected between May 2009 and November 2010 from Makkah region. This study provides data on the viral diagnosis and genetic diversity of hemagglutinin (HA) and neuraminidase (NA) genes of influenza A (H1N1)pdm09 virus from Saudi Arabia. Methodology: Nasopharyngeal swabs from 100 clinically infected patients in the peak of the outbreak were collected from Makkah region and processed for viral diagnosis by viral culture and real-time polymerase chain reaction (PCR). HA and NA genes of 10 selected samples were sequenced and analyzed. Results: A total of 100 samples were collected; only 10 samples were found to be positive for influenza A virus infection by real-time PCR. Nucleotide sequence analysis of the HA and NA genes of influenza A (H1N1) from Saudi Arabia showed significant similarities with selected isolates. The phylogenetic tree constructed for both HA and NA genes formed close clusters with selected reference isolates. Conclusions: Nucleotide sequence analysis and phylogenetic relationships of the HA and NA genes of influenza A (H1N1) virus from Saudi Arabia with selected reference isolates indicates that they were genetically close and most probably originated from influenza A(H1N1)pdm09.

scholarly journals Isolation and molecular characterization of a urease-negative Actinobacillus pleuropneumoniae mutant

2017 ◽  
Vol 30 (1) ◽  
pp. 172-174 ◽  
Author(s):  
Hiroya Ito ◽  
Sayaka Takahashi ◽  
Tetsuo Asai ◽  
Yutaka Tamura ◽  
Koshi Yamamoto
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Gene Cluster ◽  
Molecular Characterization ◽  
Dna Sequence ◽  
Actinobacillus Pleuropneumoniae ◽  
Nucleotide Sequence Analysis ◽  
Urease Gene ◽  
Negative Mutant

An atypical urease-negative mutant of Actinobacillus pleuropneumoniae serovar 2 was isolated in Japan. Nucleotide sequence analysis of the urease gene cluster revealed that the insertion of a short DNA sequence into the cbiM gene was responsible for the urease-negative activity of the mutant. Veterinary diagnostic laboratories should be watchful for the presence of aberrant urease-negative A. pleuropneumoniae isolates.

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