A Three-Stage-Integrative Approach for the Identification of Potential Hepatotoxic Compounds From Botanical Products

2011 ◽  
Vol 30 (3) ◽  
pp. 287-299 ◽  
Author(s):  
Yecheng Jin ◽  
Xiaoping Zhao ◽  
Yufeng Zhang ◽  
Xiang Li ◽  
Xiaojing Nie ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Aristolochic Acid ◽  
Herbal Medicines ◽  
Integrative Approach ◽  
Challenging Problem ◽  
Liquid Chromatography Mass Spectrometry ◽  
Toxic Compounds ◽  
Botanical Products ◽  
Diphenyl Tetrazolium Bromide

With the increasing use of herbal medicines and dietary supplements, intensive concerns about their potential toxicities have been raised. Screening and identifying the toxic compounds from these botanical products composed by hundreds of components have become a critical but challenging problem. In this study, 3 methods, including fraction separation, an in-house-developed fluorescein diacetate-based automatic microscopy screening (FAMS) platform, and liquid chromatography-mass spectrometry-based compounds identification were integrated within the Three-Stage-Integrative (TSI) approach for the identification of potential hepatotoxicants from botanical products. The sensitivity and linear range of FAMS assay was validated and compared with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay by previously reported hepatotoxic compounds. The success of TSI approach was further demonstrated by its application to Fructus aristolochiae. Aristolochic acid IVa and aristolodione were tentatively identified to be potential hepatotoxicants in this plant. These applications suggested that our TSI approach provides an effective tool for identifying potential toxic compounds from botanical products.

scholarly journals Pyrrolizidine alkaloids in honey: determination with liquid chromatography-mass spectrometry method

2018 ◽  
Vol 62 (2) ◽  
pp. 173-181 ◽  
Author(s):  
Ewelina Kowalczyk ◽  
Krzysztof Kwiatek
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Pyrrolizidine Alkaloids ◽  
Solvent Mixture ◽  
Guidance Document ◽  
Liquid Chromatography Mass Spectrometry ◽  
Spectrometry Method ◽  
Toxic Compounds ◽  
Natural Origin

AbstractIntroductionPyrrolizidine alkaloids (PAs) are probably the most widespread toxins of natural origin. More than 6,000 plant species produce these toxic compounds. Bees can forage on flowers of plants producing PAs, which leads to contamination of honey with the toxic compounds. To determine the contamination of honey with PAs, a sensitive method based on liquid chromatography coupled with mass spectrometry has been developed.Material and MethodsPAs were extracted with 0.05 M sulphuric acid and purified with MCX cartridges. A solvent mixture consisting of ethyl acetate, methanol, acetonitrile, ammonia, and triethylamine (8:1:1:0.1:0.1, v/v) was used to wash alkaloids from the cartridges. After evaporation the residues were reconstituted in water and methanol mixture and subjected to LC–MS analysis.ResultsThe developed method was validated according to SANTE/11945/2015 requirements. The recovery was from 80.6% to 114.5%. The repeatability ranged from 2.3% to 14.6%, and the reproducibility was from 4.9% to 17.7%.ConclusionsA new method for the determination of PAs in honey has been developed and validated. All evaluated parameters were in accordance with the SANTE/11945/2015 guidance document. Out of 50 analysed honey samples, 16 (32%) were positive for the content of at least one PA.

scholarly journals Analysis of Chemical Properties of Edible and Medicinal Ginger by Metabolomics Approach

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Ken Tanaka ◽  
Masanori Arita ◽  
Hiroaki Sakurai ◽  
Naoaki Ono ◽  
Yasuhiro Tezuka
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Chemical Constituents ◽  
Herbal Medicines ◽  
Chemical Properties ◽  
Liquid Chromatography Mass Spectrometry ◽  
Comprehensive Understanding ◽  
Traditional Herbal Medicine ◽  
Derivatives Of ◽  
Bioactive Constituent

In traditional herbal medicine, comprehensive understanding of bioactive constituent is important in order to analyze its true medicinal function. We investigated the chemical properties of medicinal and edible ginger cultivars using a liquid-chromatography mass spectrometry (LC-MS) approach. Our PCA results indicate the importance of acetylated derivatives of gingerol, not gingerol or shogaol, as the medicinal indicator. A newly developed ginger cultivar,Z. officinalecv. Ogawa Umare or “Ogawa Umare” (OG), contains more active ingredients, showing properties as a new resource for the production of herbal medicines derived from ginger in terms of its chemical constituents and rhizome yield.

Sign in / Sign up

Export Citation Format

Share Document