scholarly journals COMPARISON OF THE GOLD-THIOCHOLINE AND GOLD-THIOLACETIC ACID METHODS FOR THE HISTOCHEMICAL LOCALIZATION OF ACETYLCHOLINESTERASE AND CHOLINESTERASES

1966 ◽  
Vol 14 (6) ◽  
pp. 443-454 ◽  
Author(s):  
GEORGE B. KOELLE ◽  
CORNELIA G. GROMADZKI
Keyword(s):  
Electron Density ◽  
High Specificity ◽  
Potential Usefulness ◽  
Histochemical Localization ◽  
High Electron ◽  
High Electron Density ◽  
Selective Inhibitors ◽  
Electron Microscopic ◽  
Thiolacetic Acid

The thiocholine (ThCh) and thiolacetic acid (ThAc) methods for the histochemical localization of acetylcholinesterase (AChE) and cholinesterases (ChE's) were modified to permit the substitution of aurous gold for copper and lead, respectively, as the capturing agent. The chief advantage of the modifications is that the precipitates formed (AuThCh-phosphate and AuS) have fine, colloidal dimensions and high electron density, thus improving their potential usefulness as electron microscopic methods. By means of selective inhibitors of AChE (10–5 M BW 284), of ChE's ( 10–7 M DFP or 3.10–8 M Nu-683), and of both enzymes (10–5 M DFP or eserine), it was shown that the gold thiocholine (AuThCh) method retains the high specificity of the original ThCh method, whereas the gold thiolacetic acid (AuThAc) method, like the lead ThAc procedure, is considerably less specific. However, the AuThAc method appears to permit finer localization than the AuTuCh technique.

scholarly journals 1-naphthol-pyronin B as a novel substrate for silver intensification: application to light and electron microscopic immunocytochemistry of neuroendocrine systems.

1990 ◽  
Vol 38 (8) ◽  
pp. 1209-1214 ◽  
Author(s):  
R Toni ◽  
R M Lechan
Keyword(s):  
Electron Density ◽  
Paraventricular Nucleus ◽  
Tissue Section ◽  
High Electron ◽  
High Electron Density ◽  
Electron Microscopic ◽  
Electron Microscopic Immunocytochemistry ◽  
Standard Methods ◽  
Neuronal Systems ◽  
Double Immunolabeling

We describe a modification of silver intensification of immunoperoxidase end-product using 1-naphthol (1N) and 1N enhanced by pyronin B after suppressing nonspecific tissue argyrophilia with a solution of penicillamine and merthiolate buffered near neutral pH. This approach facilitates the preservation of a second antigen sequentially labeled in the same tissue section for light microscopic double immunolabeling experiments and also allows retention of ultrastructural detail. Using this protocol, we obtained rapid and uniform silver intensification of somatostatin (SRIF)-immunoreactive (IR) neuronal perikarya and processes in the rat hypothalamic paraventricular nucleus (PVN). Ultrastructurally, 1N- and 1N-pyronin B-silver intensified reaction product was clearly recognized by the presence of a coarse intracellular precipitate of high electron density. Light microscopic double-immunolabeling studies demonstrated the association between SRIF- and thyrotropin-releasing hormone (TRH)-IR neuronal systems in the PVN. We propose that silver intensification of 1N and 1N-pyronin B is a useful alternative to standard methods of silver intensification of immunoperoxidase reaction product at both light and ultrastructural levels and may be particularly amenable for double-immunolabeling studies.

Unusually high electron density in an intermolecular non-bonding region: Role of metal substrate

2017 ◽  
Vol 28 (4) ◽  
pp. 759-764 ◽  
Author(s):  
Chen-Guang Wang ◽  
Zhi-Hai Cheng ◽  
Xiao-Hui Qiu ◽  
Wei Ji
Keyword(s):  
Electron Density ◽  
Metal Substrate ◽  
High Electron ◽  
High Electron Density

New insights into electrolyte-component biased and transfer- and transport-limited charge recombination in dye-sensitized solar cells

RSC Advances ◽  
2015 ◽  
Vol 5 (103) ◽  
pp. 84959-84966 ◽  
Author(s):  
Dong-Li Gao ◽  
Yi Wang ◽  
Ping Zhang ◽  
Li-Min Fu ◽  
Xi-Cheng Ai ◽  
...  
Keyword(s):  
Solar Cells ◽  
Electron Density ◽  
Dye Sensitized Solar Cells ◽  
Charge Recombination ◽  
High Electron ◽  
High Electron Density ◽  
Dye Sensitized ◽  
Electrolyte Component ◽  
Sensitized Solar Cells

Charge recombination takes place, respectively, within the frameworks of transfer- and transport-limited recombination mechanisms, at low and high electron density.

High electron density β-(Al0.17Ga0.83)2O3/Ga2O3 modulation doping using an ultra-thin (1 nm) spacer layer

2020 ◽  
Vol 127 (21) ◽  
pp. 215706 ◽  
Author(s):  
Nidhin Kurian Kalarickal ◽  
Zhanbo Xia ◽  
Joe F. McGlone ◽  
Yumo Liu ◽  
Wyatt Moore ◽  
...  
Keyword(s):  
Electron Density ◽  
High Electron ◽  
High Electron Density ◽  
Modulation Doping ◽  
Spacer Layer

Donor-Stabilized Silylene/Phosphine-Supported Carbon(0) Center with High Electron Density

2017 ◽  
Vol 56 (24) ◽  
pp. 6891-6895 ◽  
Author(s):  
Thibault Troadec ◽  
Tatsuya Wasano ◽  
Romaric Lenk ◽  
Antoine Baceiredo ◽  
Nathalie Saffon-Merceron ◽  
...  
Keyword(s):  
Electron Density ◽  
High Electron ◽  
High Electron Density

Doping design of GaN-based heterostructure field-effect transistors with high electron density for high-power applications

2003 ◽  
Vol 200 (1) ◽  
pp. 168-174 ◽  
Author(s):  
Narihiko Maeda ◽  
Takehiko Tawara ◽  
Tadashi Saitoh ◽  
Kotaro Tsubaki ◽  
Naoki Kobayashi
Keyword(s):  
Electron Density ◽  
High Power ◽  
Field Effect ◽  
Field Effect Transistors ◽  
High Electron ◽  
High Electron Density ◽  
Heterostructure Field Effect Transistors

VARIATIONS IN SOLAR TIME OF EXTENSIVE AIR SHOWERS

1961 ◽  
Vol 39 (6) ◽  
pp. 935-940
Author(s):  
B. G. Wilson ◽  
J. G. G. Dionne ◽  
F. Terentiuk
Keyword(s):  
Electron Density ◽  
Extensive Air Showers ◽  
High Electron ◽  
High Electron Density ◽  
Air Showers ◽  
Solar Time ◽  
Wide Range

150,000 extensive air showers of high electron density have been recorded at an altitude of 2283 meters. Analysis of the data in solar time for a wide range of densities shows no evidence for the large semidiurnal variations previously reported by other groups. A possible explanation of this difference may lie in the different methods of selecting the showers.

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