scholarly journals Two New Triterpenoid Saponins from the Structural Modification of Pseudoginsenoside-F11 and their Cytotoxic Activities

2016 ◽  
Vol 11 (5) ◽  
pp. 1934578X1601100
Author(s):  
Manman Li ◽  
Zhicai Wang ◽  
Pengfei Zhang ◽  
Yan Xu ◽  
Hongda Ding ◽  
...  
Keyword(s):  
Acid Hydrolysis ◽  
Tumor Cells ◽  
Structural Modification ◽  
Human Tumor ◽  
2D Nmr ◽  
Cytotoxic Activities ◽  
Human Tumor Cells ◽  
Triterpenoid Saponins ◽  
Spectroscopic Analyses ◽  
Hydrolysis Products

Two ginsenoside derivatives (1, 2) along with 2 known ginsenosides (3, 4) were isolated from the acid hydrolysis products of pseudoginsenoside-F11. Their structures were elucidated on the basis of spectroscopic analyses, including 1D, 2D NMR and HR-ESI-MS. Among them, (12 R, 20 S, 24 S)-20, 24; 12, 24-diepoxy-dammarane-3β, 6α-diol (1) and (20 R, 24 R)-dammar-20, 24-epoxy-3β, 6α, 12β, 25-tetraol (2) were identified as new triterpenoid saponins. They were subjected to assay for cytotoxic activities against six human tumor cells lines.

Extraction, Purification and Cytotoxic Activity of Cerebrosides from Asterina pectinifera

2013 ◽  
Vol 781-784 ◽  
pp. 682-686 ◽  
Author(s):  
Hai Jiao Zuo ◽  
Tian Wang ◽  
Yan Xia Qi ◽  
Qian Cheng Zhao ◽  
Zhi Bo Li
Keyword(s):  
Thin Layer ◽  
Tumor Cells ◽  
Human Tumor ◽  
Time Dependent ◽  
Cytotoxic Activities ◽  
Dependent Manner ◽  
Human Tumor Cells ◽  
Ethanol Extraction ◽  
Asterina Pectinifera ◽  
Layer Chromatography

Cerebrosides were extracted from Asterina pectinifera using ethanol extraction and silica gel column chromatography. The identification of the cerebrosides was established based on thin layer chromatography (TLC), UV, IR and HRMS. The cerebrosides exhibited moderate cytotoxic activities against Hela and BEL7402 cell lines. At the concentration range of 10-400μg/ml, cytotoxicity of cerebrosides on two human tumor cells showed a dose-and time-dependent manner.

Inhibition by dihydrorifampicin of RNA polymerases I and II isolated from nuclei of Rous sarcoma cells and human tumor cells HEp-2

1979 ◽  
Vol 44 (9) ◽  
pp. 2722-2736 ◽  
Author(s):  
Jindřich Kára ◽  
Zdeněk Hostomský
Keyword(s):  
Tumor Cells ◽  
Human Tumor ◽  
Tumor Cell Line ◽  
Selective Inhibition ◽  
Rna Polymerases ◽  
Embryonic Cells ◽  
Human Tumor Cells ◽  
Ribonucleic Acids ◽  
Rous Sarcoma ◽  
Sarcoma Cells

Dihydrorifampicin, a rifampicin derivative hydrogenated at the 18-19 carbon atoms of the aliphatic ansa chain of the rifampicin molecule, inhibits the enzymatic activity of RNA polymerases I and II, isolated from the nuclei of avian tumor cells (Rous sarcoma) and from the human tumor cell line HEp-2. The RNA polymerases from these tumors have been separated and partially purified by chromatography on DEAE Sephadex A-25 and characterized by the sensitivity to α-amanitin. The [3H]UMP-labeled ribonucleic acids synthesized in the isolated nuclei of Rous sarcoma cells in the presence and absence of DHR were analyzed by sedimentation analysis in sucrose density gradients. It was found that the synthesis of rRNAs and mRNAs is very significantly inhibited by dihydrorifampicin, whereas the synthesis of tRNAs is much less inhibited at the same DHR concentration (100μg/ml). The observed cytostatic effect of DHR on the growth of human tumor cells HEp-2 and embryonic cells in culture is apparently mediated by the selective inhibition of RNA polymerases I and II in human and avian cells. The relationship between the molecular structure of DHR and its affinity to RNA polymerases of eukaryotic cells is discussed.

The Sensitivity of Human Tumor Cells to the Cytotoxicity of Gold Polyacrylate (Aurumacryl)

BIOPHYSICS ◽  
2019 ◽  
Vol 64 (6) ◽  
pp. 930-935 ◽  
Author(s):  
D. B. Korman ◽  
E. I. Nekrasova ◽  
L. A. Ostrovskaya ◽  
O. O. Ryabaya ◽  
N. V. Bluhterova ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Human Tumor ◽  
Human Tumor Cells
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