Silk fibroin nanoparticles for celecoxib and curcumin delivery: ROS-scavenging and anti-inflammatory activities in an in vitro model of osteoarthritis

2019 ◽  
Vol 137 ◽  
pp. 37-45 ◽  
Author(s):  
Barbara Crivelli ◽  
Elia Bari ◽  
Sara Perteghella ◽  
Laura Catenacci ◽  
Milena Sorrenti ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
In Vitro Model ◽  
Ros Scavenging ◽  
Silk Fibroin Nanoparticles ◽  
Anti Inflammatory ◽  
Vitro Model

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

Anti-inflammatory effects of dietary phenolic compounds in an in vitro model of inflamed human intestinal epithelium

2010 ◽  
Vol 188 (3) ◽  
pp. 659-667 ◽  
Author(s):  
Thérèse Sergent ◽  
Neil Piront ◽  
Julie Meurice ◽  
Olivier Toussaint ◽  
Yves-Jacques Schneider
Keyword(s):  
Phenolic Compounds ◽  
Intestinal Epithelium ◽  
In Vitro Model ◽  
Anti Inflammatory ◽  
Vitro Model ◽  
Human Intestinal Epithelium

Anti-inflammatory activity of Cannabis sativa L. extracts in an in vitro model of skin inflammation

2017 ◽  
Author(s):  
M Fumagalli ◽  
E Sangiovanni ◽  
B Pacchetti ◽  
S Piazza ◽  
M Dell'Agli
Keyword(s):  
In Vitro Model ◽  
Cannabis Sativa ◽  
Skin Inflammation ◽  
Inflammatory Activity ◽  
Anti Inflammatory ◽  
Vitro Model

scholarly journals Chondroprotective Effects of Genistein against Osteoarthritis Induced Joint Inflammation

Nutrients ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 1180 ◽  
Author(s):  
Feng-Cheng Liu ◽  
Chih-Chien Wang ◽  
Jeng-Wei Lu ◽  
Chian-Her Lee ◽  
Shao-Chi Chen ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Joint Inflammation ◽  
Pathway Activation ◽  
Anti Cancer ◽  
Oa Chondrocytes ◽  
Anti Inflammatory ◽  
Vitro Model ◽  
Nitric Oxide Synthase 2 ◽  
Oxide Synthase

Genistein is an isoflavone extracted from soybean (Glycine max). This compound has anti-inflammatory, anti-oxidative, and anti-cancer effects; however, the mechanism underlying the effects of genistein on IL-1β-stimulated human osteoarthritis (OA) chondrocytes remains unknown. Our objectives in this study were to explore the anti-inflammatory effects of genistein on IL-1β-stimulated human OA chondrocytes and to investigate the potential mechanisms which underlie them. Our results from an in-vitro model of osteoarthritis indicate that genistein inhibits the IL-1β-induced expression of the catabolic factors nitric oxide synthase 2 (NOS2), cyclooxygenase 2 (COX-2), and matrix metalloproteinases (MMPs). Genistein was shown to stimulate Ho-1 expression, which has been associated with Nrf-2 pathway activation in human chondrocytes. In a rat model, genistein was also shown to attenuate the progression of traumatic osteoarthritis. Taken together, these results demonstrate the effectiveness of genistein in mediating the inflammation associated with joint disorders. Our results also indicate that genistein could potentially serve as an alternative therapeutic treatment for OA.

Antioxidative and Anti-Inflammatory Activities of Akebia quinata Extracts in an In Vitro Model of Acute Alcohol-Induced Hepatotoxicity

2017 ◽  
Vol 20 (9) ◽  
pp. 912-922 ◽  
Author(s):  
Sang Hoon Lee ◽  
Young Sun Song ◽  
Yoonhwa Jeong ◽  
Kwang Suk Ko
Keyword(s):  
In Vitro Model ◽  
Anti Inflammatory ◽  
Vitro Model

Four new triterpenoids from Chisocheton paniculatus and their anti-inflammatory activities

2012 ◽  
Vol 90 (2) ◽  
pp. 199-204 ◽  
Author(s):  
Ming-Hua Yang ◽  
Jun-Song Wang ◽  
Jian-Guang Luo ◽  
Xiao-Bing Wang ◽  
Ling-Yi Kong
Keyword(s):  
Spectroscopic Data ◽  
In Vitro Model ◽  
2D Nmr ◽  
No Production ◽  
Moderate Activity ◽  
Inhibitory Effects ◽  
Raw264.7 Macrophages ◽  
Anti Inflammatory ◽  
Vitro Model

As part of our continuing effort to discover new potential anti-inflammatory agents, four new triterpenoids, chisopanins L-O (1–4), were isolated from the twigs of Chisocheton paniculatus. Their structures were established on the basis of detailed analysis of spectroscopic data, including IR, NMR (1D and 2D NMR), and HR-ESI-MS. Their inhibitory effects against nitric oxide (NO) production were evaluated in an in vitro model of lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Compounds 1, 3, and 4 were found to exhibit moderate activity with IC50 values less than 10 μmol/L.

Anti-inflammatory effects of pomegranate (Punica granatum L.) husk ellagitannins in Caco-2 cells, an in vitro model of human intestine

2012 ◽  
Vol 3 (8) ◽  
pp. 875 ◽  
Author(s):  
Sylvie Hollebeeck ◽  
Julie Winand ◽  
Marie-France Hérent ◽  
Alexandrine During ◽  
Joëlle Leclercq ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Punica Granatum ◽  
Human Intestine ◽  
Anti Inflammatory ◽  
Vitro Model ◽  
Punica Granatum L
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