Electroacupuncture atZusanli(ST-36) Restores Impaired Interstitial Cells of Cajal and Regulates Stem Cell Factor Pathway in the Colon of Diabetic Rats

2012 ◽  
Vol 17 (2) ◽  
pp. 117-125 ◽  
Author(s):  
Juanjuan Xu ◽  
Yan Chen ◽  
Shi Liu ◽  
Xiaohua Hou
Keyword(s):  
Stem Cell ◽  
High Frequency ◽  
Stem Cell Factor ◽  
Interstitial Cells Of Cajal ◽  
Low Frequency ◽  
Interstitial Cells ◽  
High Frequency Stimulation ◽  
Frequency Stimulation ◽  
Cells Of Cajal

The present study determined the effects of electroacupuncture on interstitial cells of Cajal and investigated whether changes in the stem cell factor pathway were involved. Animals were assigned to normal, diabetic, diabetic plus sham stimulation, diabetic plus low-frequency stimulation, and diabetic plus high-frequency stimulation groups. Electroacupuncture was performed daily for 8 weeks. In vitro contractility of colonic muscle strips were studied. Expression of c-kit (the marker of interstitial cells of Cajal) and stem cell factor were measured. The results showed that (1) contraction of colonic muscle strips was significantly elevated in low- and high-frequency stimulation groups and (2) in contrast to the diabetic group, the expressions of c-kit and stem cell factor were markedly increased in the low- and high-frequency stimulation groups. These results indicate that both low- and high-frequency stimulation can promote the contractility of colonic muscle strips partially through increasing the number of interstitial cells of Cajal, and these effects could be mediated by an elevated endogenous stem cell factor.

scholarly journals Electroacupuncture Regulates Apoptosis/Proliferation of Intramuscular Interstitial Cells of Cajal and Restores Colonic Motility in Diabetic Constipation Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Juanjuan Xu ◽  
Yan Chen ◽  
Shi Liu ◽  
Xiaohua Hou
Keyword(s):  
High Frequency ◽  
Interstitial Cells Of Cajal ◽  
Colonic Motility ◽  
Low Frequency ◽  
Interstitial Cells ◽  
Colonic Transit ◽  
Immunofluorescent Staining ◽  
High Frequency Stimulation ◽  
Frequency Stimulation ◽  
Cells Of Cajal

Injury of interstitial cells of Cajal (ICC) is associated with gut dysmotility in diabetic rats. We have shown an acceleration of the colonic contractility by electroacupuncture stimulation (EAS). However, little is known about potential roles of EAS on colonic transit and ICC. In this study, we evaluate the effect of EAS on colonic transit and investigate whether apoptosis/proliferation of ICC was involved in regulative effect of EAS on colonic transit. Rats were randomly assigned to normal, diabetic, diabetic-plus-sham stimulation, diabetic-plus-low-frequency stimulation, and diabetic-plus-high-frequency stimulation groups. Bead expulsion test was used for measuring the distal colonic transit. The Kit (ICC marker) was detected by western blot. Apoptotic ICC was detected by terminal dUTP nucleotide end labeling. Proliferating ICC was identified by Kit/Ki67 double immunofluorescent staining on whole mount preparations. Ultrastructure changes of ICC were studied using electron microscopy. Results showed that high-frequency stimulation significantly promoted colonic transit. Low- and high-frequency stimulation markedly rescued intramuscular ICC from apoptosis. Abundant proliferating intramuscular ICC was found in low- and high-frequency stimulation groups. Our results indicate that high-frequency EAS has stimulatory effect on the distal colonic transit, which may be mediated by downregulation of the apoptosis and upregulation of the proliferation of intramuscular ICC.

Roles of Stem Cell Factor on Loss of Interstitial Cells of Cajal in Bladder of Diabetic Rats

Urology ◽  
2011 ◽  
Vol 78 (6) ◽  
pp. 1443.e1-1443.e6 ◽  
Author(s):  
Wei Chen ◽  
Chonghe Jiang ◽  
Xiyu Jin ◽  
Wenhao Shen ◽  
Bo Song ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Factor ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Diabetic Rats ◽  
Cells Of Cajal

Electroacupuncture at ST36 Increases Contraction of the Gastric Antrum and Improves the SCF/c-kit Pathway in Diabetic Rats

2013 ◽  
Vol 41 (06) ◽  
pp. 1233-1249 ◽  
Author(s):  
Yan Chen ◽  
Juanjuan Xu ◽  
Shi Liu ◽  
Xiaohua Hou
Keyword(s):  
Stem Cell ◽  
Stem Cell Factor ◽  
Interstitial Cells Of Cajal ◽  
Low Frequency ◽  
Underlying Mechanism ◽  
Diabetic Rats ◽  
Gastric Antrum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Organ Bath ◽  
Cells Of Cajal

Electroacupuncture (EA) at ST36 is effective for improving gastric motility. However, the underlying mechanism remains poorly understood. The aim of this study was to investigate the effects of EA on gastric contraction and to determine whether interstitial cells of Cajal (ICCs) are involved. Rats were randomized into control, diabetic (DM), diabetic with sham EA (DM + SEA), diabetic with low frequency EA (DM + LEA) and diabetic with high frequency EA (DM + HEA) groups. EA was performed everyday for four and eight weeks. Contractions in antrum strips were explored using the organ bath technique. Western blotting was employed to determine c-kit and transmembrane stem cell factor (M-SCF) expression in the gastric antrum, and levels of soluble stem cell factor (S-SCF) in serum were determined by enzyme-linked immunosorbent assay (ELISA). The distribution of ICCs was further assessed by immunohistochemistry. The results were as follows: (1) Contractions in the DM group were attenuated at four and eight weeks, but LEA and HEA restored the attenuated contraction. (2) ICCs were significantly decreased at eight weeks without alteration at four weeks in DM group, but were rescued in the LEA and HEA groups. (3) Whereas M-SCF and S-SCF in the DM group were slightly decreased at four weeks and were dramatically reduced at eight weeks, LEA and HEA markedly enhanced SCF at eight weeks. Collectively, the data suggest that in diabetic rats, LEA and HEA at ST36 could facilitate contraction of the gastric antrum, possibly by involving the SCF/c-kit pathway.

T1364 The Effect of Stem Cell Factor On Interstitial Cells of Cajal in the Colon of Diabetic Mice

2008 ◽  
Vol 134 (4) ◽  
pp. A-539-A-540
Author(s):  
Lin Lin ◽  
Liming Xu ◽  
Yurong Tang ◽  
Hongjie Zhang ◽  
Li Xueliang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Factor ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Diabetic Mice ◽  
Cells Of Cajal

scholarly journals Construction of recombinant lentiviral vector containing human stem cell leukemia gene and its expression in interstitial cells of cajal

2020 ◽  
Vol 15 (1) ◽  
pp. 83-91
Author(s):  
Chen Li ◽  
Biao Qian ◽  
Zhao Ni ◽  
Qinzhang Wang ◽  
Zixiong Wang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Interstitial Cells Of Cajal ◽  
Lentiviral Vector ◽  
Transfection Efficiency ◽  
Interstitial Cells ◽  
Human Stem Cell ◽  
Cell Leukemia ◽  
Cells Of Cajal ◽  
Stem Cell Leukemia

AbstractThis study aims to construct recombinant lentiviral vectors containing the human stem cell leukemia (SCL) gene and investigate their in vitro transfection efficiency in Interstitial Cells of Cajal (ICC) of guinea pig bladders. In this study, the human SCL gene was successfully cloned, and the recombinant lentivirus GV287-SCL was successfully constructed. The titer of the recombinant lentivirus was 5 × 108 TU /mL. After transfecting the ICCs with the lentiviral vector at different MOIs, the optimal MOI was determined to be 10.0, and the optimal transfection time was determined to be 3 days. The amplification product of the lentivirus transfection group was consistent with the target fragment, indicating that the SCL gene had been successfully introduced into ICCs. In conclusion, the recombinant lentiviral vector GV287-SCL was successfully constructed and transfected into the in vitro cultured ICCs. The successful expression of SCL in ICCs may provide an experimental basis for the in vivo transfection of the SCL gene.

scholarly journals Effect of stem cell factor on colon interstitial cells of Cajal in murine with diabetes mellitus

2008 ◽  
Vol 16 (12) ◽  
pp. 1294
Author(s):  
Li-Ming Xu ◽  
Lin Lin ◽  
Yu-Rong Tang ◽  
Hong-Jie Zhang ◽  
Xue-Liang Li
Keyword(s):  
Diabetes Mellitus ◽  
Stem Cell ◽  
Stem Cell Factor ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Cells Of Cajal

Cellular Mechanisms Underlying Antiepileptic Effects of Low- and High-Frequency Electrical Stimulation in Acute Epilepsy in Neocortical Brain Slices In Vitro

2007 ◽  
Vol 97 (3) ◽  
pp. 1887-1902 ◽  
Author(s):  
Yitzhak Schiller ◽  
Yael Bankirer
Keyword(s):  
Electrical Stimulation ◽  
High Frequency ◽  
Brain Slices ◽  
Low Frequency ◽  
High Frequency Stimulation ◽  
Dependent Manner ◽  
Cellular Mechanisms ◽  
Epileptiform Discharges ◽  
Frequency Stimulation

Approximately 30% of epilepsy patients suffer from drug-resistant epilepsy. Direct electrical stimulation of the epileptogenic zone is a potential new treatment modality for this devastating disease. In this study, we investigated the effect of two electrical stimulation paradigms, sustained low-frequency stimulation and short trains of high-frequency stimulation, on epileptiform discharges in neocortical brain slices treated with either bicuculline or magnesium-free extracellular solution. Sustained low-frequency stimulation (5–30 min of 0.1- to 5-Hz stimulation) prevented both interictal-like discharges and seizure-like events in an intensity-, frequency-, and distance-dependent manner. Short trains of high-frequency stimulation (1–5 s of 25- to 200-Hz stimulation) prematurely terminated seizure-like events in a frequency-, intensity-, and duration-dependent manner. Roughly one half the seizures terminated within the 100-Hz stimulation train ( P < 0.01 compared with control), whereas the remaining seizures were significantly shortened by 53 ± 21% ( P < 0.01). Regarding the cellular mechanisms underlying the antiepileptic effects of electrical stimulation, both low- and high-frequency stimulation markedly depressed excitatory postsynaptic potentials (EPSPs). The EPSP amplitude decreased by 75 ± 3% after 10-min, 1-Hz stimulation and by 86 ± 6% after 1-s, 100-Hz stimulation. Moreover, partial pharmacological blockade of ionotropic glutamate receptors was sufficient to suppress epileptiform discharges and enhance the antiepileptic effects of stimulation. In conclusion, this study showed that both low- and high-frequency electrical stimulation possessed antiepileptic effects in the neocortex in vitro, established the parameters determining the antiepileptic efficacy of both stimulation paradigms, and suggested that the antiepileptic effects of stimulation were mediated mostly by short-term synaptic depression of excitatory neurotransmission.

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