scholarly journals Immunolocalization of the protease kallikrein in the colon.

1983 ◽  
Vol 31 (11) ◽  
pp. 1255-1260 ◽  
Author(s):  
M Schachter ◽  
M W Peret ◽  
A G Billing ◽  
G D Wheeler
Keyword(s):  
Gastrointestinal Tract ◽  
Serine Proteases ◽  
Goblet Cells ◽  
Physiological Significance ◽  
Immunocytochemical Techniques ◽  
The Many

Colon kallikrein was localized in the goblet cells of cat and man by a variety of immunocytochemical techniques. No evidence of this enzyme was found in other sites in this organ. The possible physiological significance of kallikrein in the gastrointestinal tract and of the many related serine proteases is discussed.

The many functions of zinc in inflammatory conditions of the gastrointestinal tract

2000 ◽  
Vol 13 (1) ◽  
pp. 33-39 ◽  
Author(s):  
G.C. Sturniolo ◽  
V. Di Leo ◽  
M. Barollo ◽  
W. Fries ◽  
E. Mazzon ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Inflammatory Conditions ◽  
The Many

scholarly journals Expression, localization and potential physiological significance of alcohol dehydrogenase in the gastrointestinal tract

2003 ◽  
Vol 270 (12) ◽  
pp. 2652-2662 ◽  
Author(s):  
Julia Vaglenova ◽  
Susana E. Martinez ◽  
Sergio Porte ◽  
Gregg Duester ◽  
Jaume Farres ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Alcohol Dehydrogenase ◽  
Physiological Significance

Drugs in gastrointestinal care

2021 ◽  
pp. 405-428
Author(s):  
Jennie Burch ◽  
Brigitte Collins
Keyword(s):  
Clinical Practice ◽  
Gastrointestinal Tract ◽  
Side Effects ◽  
Proton Pump Inhibitors ◽  
Nursing Care ◽  
Proton Pump ◽  
Gastrointestinal Diseases ◽  
Ease Of Use ◽  
The Many ◽  
Upper Gastrointestinal

The chapter entitled drugs in gastrointestinal care explores the many drugs that are either used in the care of people with disorders and diseases of the gastrointestinal tract or affect the gut. Medications are often necessary to treat gastrointestinal issues but there may be side effects that should be managed by nurses, Pain might be treated by drugs, such as different types of analgesia; infections are treated by antibiotics, and nausea by antiemetics. Additionally treatment of constipation with the use of laxatives is explored as there are a variety of medications that have dissimilar methods of working. Upper gastrointestinal diseases may be treated by medications, such as proton pump inhibitors. Alternatively, there may be side effects of other drugs that affect the gastrointestinal tract that require nursing care. This chapter is tabulated for ease of use by the nurse in clinical practice, to include the indication for when to use the drug and any side effects.

scholarly journals TURNOVER OF S35-SULFATE IN THE MUCOSA OF THE GASTROINTESTINAL TRACT OF RATS AS SEEN IN AUTORADIOGRAMS

1956 ◽  
Vol 2 (1) ◽  
pp. 29-32 ◽  
Author(s):  
Dominic D. Dziewiatkowski
Keyword(s):  
Gastrointestinal Tract ◽  
Goblet Cells ◽  
Intestinal Wall ◽  
Intestinal Lumen ◽  
Water Contact ◽  
Intense Reaction ◽  
Lower Intestine

Segments of the gastrointestinal tract removed from rats after intervals of time following injection of S35-sulfate were fixed in aqueous formalin and then washed in water. Contact and coated autoradiograms were prepared. The suggestion made by others that more of the labelled sulfate is fixed by the mucosa than by the underlying coats of the gastrointestinal tract is confirmed. In addition it was found that the isotope is fixed to a greater extent in the lower intestine than in the middle or upper portions of it. Coated autoradiograms revealed that 6 hours after administration of S35-sulfate more of the label was present in the goblet cells lying deep in the crypts of the mucosa than in those adjacent to the intestinal lumen. By the 24th hour the concentration of the isotope was strikingly higher and more uniform from cell to cell. The mucus in the intestinal lumen was also highly radioactive. At the end of 48 hours very little of the sulfur-35 remained in the intestinal wall or could be made out in the mucus of the lumen: the autoradiographic reaction was faint and diffuse as contrasted with the punctiform and intense reaction given by the specimens removed at the end of shorter intervals of time.

The zinc-finger transcription factor Klf4 is required for terminal differentiation of goblet cells in the colon

Development ◽  
2002 ◽  
Vol 129 (11) ◽  
pp. 2619-2628 ◽  
Author(s):  
Jonathan P. Katz ◽  
Nathalie Perreault ◽  
Bree G. Goldstein ◽  
Catherine S. Lee ◽  
Patricia A. Labosky ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Transcription Factor ◽  
Gastrointestinal Tract ◽  
Epithelial Cell ◽  
Zinc Finger ◽  
Goblet Cell ◽  
Goblet Cells ◽  
Specific Marker ◽  
Zinc Finger Transcription Factor

Klf4 (formerly GKLF) is a zinc-finger transcription factor expressed in the epithelia of the skin, lungs, gastrointestinal tract and several other organs. In vitro studies have suggested that Klf4 plays an important role in cell proliferation and/or differentiation. Mice homozygous for a null mutation in Klf4 die within 15 hours of birth and show selective perturbation of late-stage differentiation structures in the epidermis, but the function of Klf4 in the gastrointestinal tract has not been investigated. To address this issue, we have generated Klf4–/– mice by homologous recombination in embryonic stem cells. In this study, we provide the first in vivo evidence that Klf4 is a goblet cell-specific differentiation factor in the colon. Klf4–/– mice exhibit normal cell proliferation and cell death rates in the colon on postnatal day 1. However, Klf4–/– mice demonstrate a 90% decrease in the number of goblet cells in the colon, show abnormal expression of the goblet cell-specific marker Muc2 by in situ hybridization, have abnormal staining of the colonic epithelium with Alcian Blue for acidic mucins, and lack normal goblet cell morphology by ultrastructural analysis. All other epithelial cell types are present in the colon of Klf4–/– mice. In summary, Klf4 plays a crucial role in colonic epithelial cell differentiation in vivo.

scholarly journals Immunocytochemical and enzyme histochemical localization of kallikrein-like enzymes in colon, intestine, and stomach of rat and cat.

1986 ◽  
Vol 34 (7) ◽  
pp. 927-934 ◽  
Author(s):  
M Schachter ◽  
D J Longridge ◽  
G D Wheeler ◽  
J G Mehta ◽  
Y Uchida
Keyword(s):  
Mast Cell ◽  
Small Intestine ◽  
Enzyme Histochemistry ◽  
Goblet Cells ◽  
Rat Colon ◽  
Mucous Cells ◽  
Cell Enzyme ◽  
Immunocytochemical Techniques ◽  
Positive Results ◽  
Histochemical Procedure

Kallikrein was localized in goblet (or mucous) cells of rat colon and in rat and cat small intestine and stomach by two immunocytochemical techniques. A kallikrein-like enzyme was also localized by enzyme histochemistry in mast cells of colon, intestine, and stomach of the cat, where they appeared to be associated with blood vessels in the lamina propria. The mast cell enzyme, however, was not detected by immunocytochemistry using antibodies to kallikrein. Modification in the enzyme histochemical procedure (pH, fixation) yielded positive results for a kallikrein-like protease in goblet cells of the intestine and colon. The possible physiological and pathological significance of kallikrein-like enzyme in the gastrointestinal tract and elsewhere is discussed.

scholarly journals The mucus and mucins of the goblet cells and enterocytes provide the first defense line of the gastrointestinal tract and interact with the immune system

2014 ◽  
Vol 260 (1) ◽  
pp. 8-20 ◽  
Author(s):  
Thaher Pelaseyed ◽  
Joakim H. Bergström ◽  
Jenny K. Gustafsson ◽  
Anna Ermund ◽  
George M. H. Birchenough ◽  
...  
Keyword(s):  
Immune System ◽  
Gastrointestinal Tract ◽  
Goblet Cells

scholarly journals Light microscopic and immunohistochemical study of the trachea of the broad-snouted caiman (Caiman latirostris)

2011 ◽  
Vol 56 (No. 1) ◽  
pp. 49-54 ◽  
Author(s):  
CM Santos ◽  
M. Abidu-Figueiredo ◽  
MJ Teixeira ◽  
AA Nascimento ◽  
A. Sales
Keyword(s):  
Connective Tissue ◽  
Lamina Propria ◽  
Endocrine Cells ◽  
Hyaline Cartilage ◽  
Immunohistochemical Study ◽  
Goblet Cells ◽  
Cytoplasmic Process ◽  
Elastic Fibres ◽  
Immunocytochemical Techniques ◽  
Alpha Actin

The purpose of this study was to examine the tracheal structure of the crocodile Caiman latirostris using light microscopy, histochemical and immunocytochemical techniques. The tracheal epithelium of C. latirostris consists of a ciliated pseudostratified columnar epithelium with goblet cells. The respiratory epithelium also includes endocrine cells immunoreactive to serotonin. The histochemical techniques demonstrated the presence of neutral and sulphated mucins secreted by goblet cells. The lamina propria consists of connective tissue with many reticular fibres. The elastic fibres are interspersed among collagen bundles, forming the border between the mucosa and the submucosa. The submucosal layer consists of connective tissue similar to that found in the lamina propria. Serous or mucous glands were not observed. The predominant characteristic in the adventitia is the presence of an incomplete hyaline cartilage ring, in the form of a circle. Dense connective tissue fills the space between the extremities of each cartilage ring. Serotonin-immunoreactive cells frequently had an apical cytoplasmic process directed towards the lumen, and were therefore classified as open type. The α-actin immunohistochemistry revealed smooth muscle cells only in blood vessel walls, confirming the absence of a tracheal muscle.

Sign in / Sign up

Export Citation Format

Share Document