scholarly journals Immunogold-silver staining: new method of immunostaining with enhanced sensitivity.

1983 ◽  
Vol 31 (7) ◽  
pp. 938-944 ◽  
Author(s):  
C S Holgate ◽  
P Jackson ◽  
P N Cowen ◽  
C C Bird
Keyword(s):  
Silver Staining ◽  
New Method ◽  
Precipitation Reaction ◽  
Human Tonsil ◽  
Fixed Tissue ◽  
Immunogold Staining ◽  
Enhanced Sensitivity ◽  
Secondary Antiserum ◽  
Silver Staining Method ◽  
Staining Methods

A new method for demonstrating antigens in paraffin sections of formol sublimate-fixed tissue is described that utilizes an "indirect" immunohistological technique employing immunoglobulin adsorbed to colloidal gold as the secondary antiserum. The gold particles introduced to antigenic sites are revealed by a silver precipitation reaction. This technique, the immunogold-silver staining method, is of much enhanced sensitivity (up to 200-fold) as compared with standard immunoperoxidase and immunogold staining methods. The results have been confirmed in a study of immunoglobulins in reactive human tonsil. The use of this new method for double immunolabeling is also described.

An acidic silver staining method for synaptonemal complexes under light microscopy

1984 ◽  
Vol 133 (1) ◽  
pp. 99-102 ◽  
Author(s):  
José Fernandez-Piqueras ◽  
Carlos Sentis Castaño ◽  
E. Rojo Garcia ◽  
A. Rodriguez Campos
Keyword(s):  
Light Microscopy ◽  
Silver Staining ◽  
Staining Method ◽  
Synaptonemal Complexes ◽  
Silver Staining Method

scholarly journals Characterization and immunocytochemical distribution of calmodulin in higher plant endosperm cells: localization in the mitotic apparatus.

1985 ◽  
Vol 101 (2) ◽  
pp. 488-499 ◽  
Author(s):  
M Vantard ◽  
A M Lambert ◽  
J De Mey ◽  
P Picquot ◽  
L J Van Eldik
Keyword(s):  
Animal Cell ◽  
Regulatory Mechanisms ◽  
Present Observation ◽  
Chromosome Movement ◽  
Animal Cells ◽  
Higher Plant ◽  
Mitotic Apparatus ◽  
Immunogold Staining ◽  
Staining Methods ◽  
Spindle Development

In this study we have examined the immunocytochemical distribution of calmodulin during mitosis of higher plant endosperm cells. Spindle development in these cells occurs without centrioles. Instead, asterlike microtubule converging centers appear to be involved in establishing spindle polarity. By indirect immunofluorescence and immunogold staining methods with anti-calmodulin antibodies, we found endosperm calmodulin to be associated with the mitotic apparatus, particularly with asterlike and/or polar microtubule converging centers and kinetochore microtubules, in an immunocytochemical pattern distinct from that of tubulin. In addition, endosperm calmodulin and calcium showed analogous distribution profiles during mitosis. Previous reports have demonstrated that calmodulin is associated with the mitotic apparatus in animal cells. The present observation that calmodulin is also associated with the mitotic apparatus in acentriolar, higher plant endosperm cells suggests that some of the regulatory mechanisms involved in spindle formation, microtubule disassembly, and chromosome movement in plant cells may be similar to those in animal cells. However, unlike animal cell calmodulin, endosperm calmodulin appears to associate with kinetochore microtubules throughout mitosis, which suggests a specialized role for higher plant calmodulin in the regulation of kinetochore microtubule dynamics.

Some new methods for studying intestinal amoebae and other protozoa

Parasitology ◽  
1942 ◽  
Vol 34 (1) ◽  
pp. 101-112 ◽  
Author(s):  
Clifford Dobell
Keyword(s):  
New Method ◽  
New Methods ◽  
General Utility ◽  
Staining Methods

During the last few years I have successfully used a new method for investigating intestinal amoebae in cultures, and in the hope that it may also prove serviceable to other workers I now propose to describe it. At the same time I take the opportunity of noting two new staining methods which I have used especially in conjunction with this technique, but which are of general utility in protozoology and cytology.

Selective silver-staining methods for RNA and proteins in the same polyacrylamide gels

1988 ◽  
Vol 169 (2) ◽  
pp. 234-238 ◽  
Author(s):  
A. Paleologue ◽  
J.P. Reboud ◽  
A.M. Reboud
Keyword(s):  
Silver Staining ◽  
Polyacrylamide Gels ◽  
Staining Methods

scholarly journals Ultrastructural cytochemical localizations by back-scattered electron imaging of white blood cells.

1981 ◽  
Vol 29 (9) ◽  
pp. 1071-1079 ◽  
Author(s):  
D Soligo ◽  
E de Harven
Keyword(s):  
Acid Phosphatase ◽  
Blood Cells ◽  
Silver Staining ◽  
White Blood Cells ◽  
Scattered Electron ◽  
Cell Surface Structures ◽  
Endogenous Peroxidase ◽  
Silver Staining Method ◽  
Electron Imaging ◽  
Scanning Electron

White blood cells have been studied in the back-scattered electron imaging (BEI) mode of scanning electron microscopy (SEM) with cytochemical methods for endogenous peroxidase, acid phosphatase, and a silver-staining method for nuclei. Peroxidase-positive granules were seen with good contrast and resolution in myeloid precursor cells and acid phosphatase activity was easily detected in macrophages and monoblasts. Silver staining permitted recognition of the shapes and location of the nuclei. In spite of the cytochemical procedures, cell surface structures were reasonably well-preserved in all methods, making direct correlation of BEI and secondary electron imaging (SEI) images an attractive feature in cell research with the scanning electron microscope.

Black mulberries (Morus nigra) as a natural dye for animal tissues staining

2011 ◽  
Vol 61 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Ehab Tousson ◽  
Bahija Al-Behbehani
Keyword(s):  
Staining Method ◽  
Physiological Saline ◽  
Natural Dyes ◽  
New Method ◽  
Beet Root ◽  
Morus Nigra ◽  
Whole Mount ◽  
Physiological Saline Solution ◽  
Staining Methods ◽  
Liver Flukes

AbstractNatural dyes produce an extraordinary diversity of rich and complex colours as well as unexpected results, making them exciting to use. Natural dyes have been used for staining wool, silk, carpet and cotton. Black mulberry (Morus nigra) has strong staining activity and a distinct flavor with juicy and acidic characteristics making them attractive for use in the processing industry in products such as fruit juice, ice cream, jelly, and jam. Aim of this study was to investigate a new staining method using black mulberry for whole mount and transverse sections staining of fasciola. Adult liver flukes (Fasciola sp.) were collected from the livers of naturally infected cows at slaughterhouse, washed with physiological saline solution. Some adult Fasciola were collected, immersed in 10% neutral buffered formalin for fixation, and embedded in paraffin for histological studies. The rest of whole mount of adult worms were collected, and then stained by the new method (dye extracted form beet root) and Carmine staining method for control. Sections, 7-10 micrometer from adult worms were collected, and then stained by the new method and hematoxyllin & eosin staining method for control. By using the dye extracted from beet root, zoologists and parasitologists can make identification and differentiation between different parasites. By using the dye extracted from black mulberry, zoologists and parasitologists can make identification and differentiation between different parasites. This dye method can be an alternative to cost and time consuming current chemical staining methods.

Nucleolar organizer activity at meiosis in wheat–rye hybrid plants

1986 ◽  
Vol 28 (2) ◽  
pp. 227-234 ◽  
Author(s):  
N. Cuñado ◽  
M. C. Cermeño ◽  
J. Orellana
Keyword(s):  
Silver Staining ◽  
Staining Method ◽  
Nucleolar Organizer ◽  
The Other ◽  
Nucleolar Organizer Regions ◽  
Binucleate Cells ◽  
Hybrid Plants ◽  
Nucleolar Organizer Activity ◽  
Silver Staining Method ◽  
Organizer Activity

Nucleoli and nucleolar organizer regions (NORs) have been studied by a silver staining method in all meiotic stages of wheat–rye hybrid plants. The maximum number of nucleoli per cell scored at meiotic prophase and tapetum binucleate cells indicates that only the NORs of 1B, 6B, and 5D wheat chromosomes are active, whereas that of chromosome IR (SAT) of rye is inactive. However, at diakinesis, metaphase and anaphase meiotic stages only chromosomes 1B and 6B show Ag-NOR as was reported previously in somatic metaphase. The absence of Ag-NOR on chromosome 5D has been imputed to its low nucleolar organizer activity, not detectable by silver staining, because of the small number of rDNA clusters it carries. On the other hand, the meiotic behaviour of chromosomes 1B and 6B has been studied at metaphase I and anaphase I, using the Ag-NORs as cytological markers.Key words: nucleolar organizer, Ag-NOR, meiosis, wheat–rye hybrids.

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