scholarly journals Immunocytochemical localization of glucocorticoid receptors in midgestation murine embryos and human embryonic cultured cells.

1984 ◽  
Vol 32 (11) ◽  
pp. 1234-1237 ◽  
Author(s):  
C S Kim ◽  
J M Lauder ◽  
T H Joh ◽  
R M Pratt
Keyword(s):  
Cleft Palate ◽  
Glucocorticoid Receptors ◽  
Normal Development ◽  
Cultured Cells ◽  
Mesenchymal Cells ◽  
Immunocytochemical Localization ◽  
Secondary Palate ◽  
Murine Embryos ◽  
Mesenchyme Cells

Glucocorticoid receptors have been localized immunocytochemically in the developing mouse secondary palatal shelves and in cultured human embryonic palatal mesenchyme cells. In the midgestation embryo, receptors are found in the highest concentration in the palatal mesenchymal cells, suggesting that they play a major role in normal development as well as in glucocorticoid-induced cleft palate. The presence of these receptors in cultured human embryonic palatal cells also suggests that development of the human secondary palate may be dependent on glucocorticoids.

Inactivation of Fgfr2 gene in mouse secondary palate mesenchymal cells leads to cleft palate

2018 ◽  
Vol 77 ◽  
pp. 137-142 ◽  
Author(s):  
Jiu-Zhen Jin ◽  
Zhenmin Lei ◽  
Zi-Jian Lan ◽  
Partha Mukhopadhyay ◽  
Jixiang Ding
Keyword(s):  
Cleft Palate ◽  
Mesenchymal Cells ◽  
Fgfr2 Gene ◽  
Secondary Palate

Biochemical and Immunocytochemical Localization of Heat-Shock Proteins in Drosophila Cultured Cells

1985 ◽  
Vol 455 (1 Intermediate) ◽  
pp. 712-714 ◽  
Author(s):  
ROBERT M. TANGUAY ◽  
JEAN-LOUP DUBAND ◽  
FRANCINE LETTRE ◽  
JEAN-PAUL VALET ◽  
ANDRÉ PATRICK ARRIGO ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Cultured Cells ◽  
Immunocytochemical Localization ◽  
Shock Proteins

scholarly journals Nuclear distribution of transcription factors in relation to sites of transcription and RNA polymerase II

1997 ◽  
Vol 110 (15) ◽  
pp. 1781-1791 ◽  
Author(s):  
M.A. Grande ◽  
I. van der Kraan ◽  
L. de Jong ◽  
R. van Driel
Keyword(s):  
Transcription Factors ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Transcription Initiation ◽  
Glucocorticoid Receptors ◽  
Cultured Cells ◽  
Spatial Relationship ◽  
Immunofluorescence Labelling ◽  
Transcription Sites ◽  
Nuclear Domains

We have investigated the spatial relationship between sites containing newly synthesized RNA and domains containing proteins involved in transcription, such as RNA polymerase II and the transcription factors TFIIH, Oct1, BRG1, E2F-1 and glucocorticoid receptors, using dual immunofluorescence labelling followed by confocal microscopy on cultured cells. As expected, a high degree of colocalisation between the RNA polymerase II and sites containing newly synthesised RNA was observed. Like the newly synthesised RNA and the RNA polymerase II, we found that all the transcription factors that we studied are distributed more or less homogeneously throughout the nucleoplasm, occupying numerous small domains. In addition to these small domains, TFIIH was found concentrated in coiled bodies and Oct1 in a single large domain of about 1.5 microm in 30% of the cells in an asynchronous HeLa cell culture. Remarkably, we found little or no relationship between the spatial distribution of the glucocorticoid receptor, Oct1 and E2F-1 on the one hand and RNA polymerase II and transcription sites on the other hand. In contrast, a significant but incomplete overlap was observed between the spatial distributions of transcription sites and BRG1 and TFIIH. These results indicate that many of the transcription factor-rich nuclear domains are not actively involved in transcription. They may represent incomplete transcription initiation complexes, inhibitory complexes, or storage sites.

Effect of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on Cleft Palate-related Genes in Mouse Embryonic Palatal Mesenchymal Cells

2006 ◽  
Vol 18 (2) ◽  
pp. 93-98 ◽  
Author(s):  
Tomohiro Yamada ◽  
Katsuaki Mishima ◽  
Kumiko Fujiwara ◽  
Hideto Imura ◽  
Norifumi Moritani ◽  
...  
Keyword(s):  
Cleft Palate ◽  
Mesenchymal Cells ◽  
Tetrachlorodibenzo P Dioxin

Modified Von Langenbeck Cleft Palate Repair Using an Anterior Triangular Flap: Decreased Incidence of Anterior Oronasal Fistulas

2009 ◽  
Vol 46 (3) ◽  
pp. 299-304 ◽  
Author(s):  
Tara Lynn Stewart ◽  
David M. Fisher ◽  
Jaret L. Olson
Keyword(s):  
Cleft Palate ◽  
Anterior Margin ◽  
Fistula Formation ◽  
Anterior Portion ◽  
Oronasal Fistula ◽  
Triangular Flap ◽  
Secondary Palate ◽  
Nasal Side ◽  
Cleft Palate Repair ◽  
Hypernasal Speech

Objective: A complication following cleft palate surgery is the development of oronasal fistulas. Despite recent advances aimed at addressing this concern, rates of postoperative fistulas have remained unchanged and are reported at between 3% and 60%. Oronasal fistulas commonly occur between the hard and soft palate and at the anterior portion of the cleft. These fistulas lead to functional problems with nasal emission, hypernasal speech, and food regurgitation through the nose. For clefts of the secondary palate, we developed a modification of the Von Langenbeck technique in which an anterior triangular flap is used to decrease the incidence of postoperative fistulas. Method: A triangular flap composed of oromucosa was designed for isolated clefts of the secondary palate only. It is based at the anterior margin of the cleft and is used as a turnover flap to allow closure of the often very tight anterior nasal side. A retrospective chart analysis was performed from 2000 to 2007. All patients who had isolated clefts of the secondary palate and had undergone a modified Von Langenbeck procedure were included in the study. Patients were evaluated 4 to 8 weeks postoperatively for the presence of oronasal fistulas. Results: With the introduction of the anterior triangular flap, we show that 0 of 182 patients developed a postoperative oronasal fistula. Conclusions: This modification of the standard Von Langenbeck uses an anterior triangular flap and confers the advantage of assisting in nasal side closure of the anterior margin of the cleft; in doing so, it reduces the rate of fistula formation.

scholarly journals Ectopic Hedgehog Signaling Causes Cleft Palate and Defective Osteogenesis

2018 ◽  
Vol 97 (13) ◽  
pp. 1485-1493 ◽  
Author(s):  
N.L. Hammond ◽  
K.J. Brookes ◽  
M.J. Dixon
Keyword(s):  
Cleft Palate ◽  
Hedgehog Signaling ◽  
Regulatory Networks ◽  
Neural Crest Cell ◽  
Bmp Signaling ◽  
The Novel ◽  
Morphogenetic Protein ◽  
Secondary Palate ◽  
Hh Signaling ◽  
Crest Cell

Cleft palate is a common birth defect that frequently occurs in human congenital malformations caused by mutations in components of the Sonic Hedgehog (S HH) signaling cascade. Shh is expressed in dynamic, spatiotemporal domains within epithelial rugae and plays a key role in driving epithelial-mesenchymal interactions that are central to development of the secondary palate. However, the gene regulatory networks downstream of Hedgehog (Hh) signaling are incompletely characterized. Here, we show that ectopic Hh signaling in the palatal mesenchyme disrupts oral-nasal patterning of the neural crest cell–derived ectomesenchyme of the palatal shelves, leading to defective palatine bone formation and fully penetrant cleft palate. We show that a series of Fox transcription factors, including the novel direct target Foxl1, function downstream of Hh signaling in the secondary palate. Furthermore, we demonstrate that Wnt/bone morphogenetic protein (BMP) antagonists, in particular Sostdc1, are positively regulated by Hh signaling, concomitant with downregulation of key regulators of osteogenesis and BMP signaling effectors. Our data demonstrate that ectopic Hh-Smo signaling downregulates Wnt/BMP pathways, at least in part by upregulating Sostdc1, resulting in cleft palate and defective osteogenesis.

Palatal Morphology and Type of Clefting

1996 ◽  
Vol 33 (6) ◽  
pp. 477-482 ◽  
Author(s):  
Pauli V.J. Kilpeläinen ◽  
Maija T. Laine-Alava ◽  
Seppo Lammi
Keyword(s):  
Cleft Palate ◽  
Developmental Stage ◽  
Multiple Regression ◽  
Regression Analyses ◽  
Missing Teeth ◽  
Secondary Palate ◽  
Different Types ◽  
Primary Palate

In this study, the variation in palatal dimensions, particularly for height, width, and depth, were evaluated in subjects with different types of cleft palate using the moiré technique. The sample consisted of 95 subjects with cleft palate and 68 noncleft individuals. Effects of cleft type, gender, developmental stage of the dentition, missing teeth, and 11 skeletal and pharyngeal cephalometric variables on palatal dimensions were assessed using multiple-regression analyses. The typing of clefts had an effect on the all palatal dimensions. The palate was shallower in subjects with clefts involving the secondary palate than in other types of clefts or in none left individuals. The palatal width was decreased in subjects with cleft in the primary palate, and the palatal depth was affected by all cleft types except submucous cleft. Gender, developmental stage of the dentition, missing teeth, and some of the cephalometric variables also had a certain effect on the palatal dimensions.

scholarly journals Mesenchymal cells with leukocyte and lymphendothelial characteristics in murine embryos

2006 ◽  
Vol 235 (6) ◽  
pp. 1554-1562 ◽  
Author(s):  
Kerstin Buttler ◽  
Alice Kreysing ◽  
Constantin S. von Kaisenberg ◽  
Lothar Schweigerer ◽  
Nick Gale ◽  
...  
Keyword(s):  
Mesenchymal Cells ◽  
Murine Embryos

Hemipalate Deformity Caused by a Teratoma

2009 ◽  
Vol 46 (2) ◽  
pp. 212-214 ◽  
Author(s):  
Craig Wittstock ◽  
Efthimios Christofides ◽  
Laurence Chait
Keyword(s):  
Cleft Palate ◽  
Normal Development ◽  
Germ Layers

Congenital intraoral tumors derived from embryological germ layers are very rare. These lesions include teratomas, dermoids, epignathi, and teratoid tumors. These embryological neoplasms may interfere with normal development and elevation of the palatal shelves, resulting in a cleft palate. We report the case of a patient with an intraoral teratoma associated with a hemipalate deformity and describe the treatment that was carried out. The classification and sometimes the confusing terminology associated with these lesions are discussed, as are the principles of managing them.

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