scholarly journals Immunohistochemical localization of Na+,K+-ATPase in rodent and human salivary and lacrimal glands.

1988 ◽  
Vol 36 (9) ◽  
pp. 1139-1145 ◽  
Author(s):  
D C Winston ◽  
R A Hennigar ◽  
S S Spicer ◽  
J R Garrett ◽  
B A Schulte
Keyword(s):  
Submandibular Gland ◽  
Immunohistochemical Localization ◽  
Secretory Cells ◽  
Mucous Cells ◽  
Duct System ◽  
Lacrimal Glands ◽  
Low Protein ◽  
Rats And Mice ◽  
Rat Submandibular Gland ◽  
Gland Type

The enzyme Na+,K+-ATPase was localized immunohistochemically in major salivary glands of mouse, rat, and human and in exorbital lacrimal glands of the rodents. Immunoreactive Na+,K+-ATPase was abundant in the basolateral membranes of all epithelial cells lining striated and intra- and interlobular ducts of all glands. Reactivity of intercalated ducts varied among gland type and species. Cells lining granular ducts in rodent submandibular gland showed a heterogeneous staining pattern in rat but stained homogeneously in mouse. Secretory cells varied greatly in their content of immunoreactive Na+,K+-ATPase. As with all duct cells, staining was present only at the basolateral surface and was never observed at the luminal surface of reactive secretory cells. Mucous cells failed to show any reactivity in any gland examined. Serous cells showed a gradient of immunostaining intensity ranging from strongly positive in demilunes of human sublingual gland to negative in rat submandibular gland and lacrimal glands of rats and mice. The presence of basolaterally localized Na+,K+-ATPase in most serous cells but not in mucous cells suggests that the enzyme contributes to the ion and water content of copious, low-protein serous secretions. The intense immunostaining of cells in most if not all segments of the duct system supports the idea that the ducts are involved with modification of the primary saliva, and extends this concept to include all segments of the duct system.

Immunohistochemical localization of kallikreins in the rat submandibular gland

Life Sciences ◽  
1975 ◽  
Vol 16 (5) ◽  
pp. 789-790
Author(s):  
Torill Berg Ørstavik ◽  
Per Brandtzaeg ◽  
Kjell Nustad ◽  
Kaare Gautvik
Keyword(s):  
Submandibular Gland ◽  
Immunohistochemical Localization ◽  
Rat Submandibular Gland

scholarly journals Immunohistochemical localization of rat submandibular gland esterase B (homologous to the RSKG-7 kallikrein gene) in relation to other serine proteases of the kallikrein family.

1992 ◽  
Vol 40 (1) ◽  
pp. 83-92 ◽  
Author(s):  
T Berg ◽  
I Wassdal ◽  
K Sletten
Keyword(s):  
Submandibular Gland ◽  
Serine Proteases ◽  
Cross Reactivity ◽  
Immunohistochemical Localization ◽  
Staining Reaction ◽  
Anatomic Distribution ◽  
Family Gene ◽  
Terminal Amino ◽  
Rat Submandibular Gland

The rat submandibular gland contains several members of the kallikrein family. In the present study we purified and raised an antiserum against one of these enzymes, i.e., esterase B, which was first described by Khullar et al. in 1986. N-terminal amino acid analysis revealed complete homology between esterase B and the kallikrein family gene RSKG-7. For characterization of the antiserum, flat-bed isoelectrofocusing with immunoblotting was superior to immunoelectrophoresis and double immunodiffusion in detecting and identifying crossreacting proteins. This was due to the fact that kallikrein-like enzymes were readily separated by isoelectrofocusing, and immunoreactivity was easily detected by the sensitive peroxidase-anti-peroxidase staining after blotting onto nitrocellulose membrane. Immunohistochemical controls were carried out accordingly, including homologous as well as crossreacting antigens. In the submandibular gland, esterase B was detected exclusively in all granular convoluted tubular cells, co-localized with tissue kallikrein and tonin. Some staining was also observed in striated duct cells; however, this staining reaction was induced by cross-reactivity with kallikrein, since staining was abolished by addition of kallikrein as well as esterase B to the primary antiserum. It was therefore concluded that like tonin and antigen gamma, but unlike kallikrein, esterase B was not detected in the striated ducts of the submandibular, parotid, or sublingual glands. This separation in anatomic distribution between esterase B and kallikrein may indicate that prokallikrein activation is not the only biological function of esterase B.

Developmental Distribution of Microperoxisomes in the Rat Submandibular Gland

1977 ◽  
Vol 35 ◽  
pp. 426-427
Author(s):  
B.A. Mooradian ◽  
L.S. Cutler
Keyword(s):  
Postnatal Development ◽  
Submandibular Gland ◽  
Cell Types ◽  
Functional Differentiation ◽  
Secretory Cells ◽  
Ductal Cells ◽  
Size Number ◽  
Rat Submandibular Gland

In the mature rat submandibular gland (SMG) microperoxisomes have been identified in both acinar secretory cells and ductal cells. The present study was undertaken to investigate the size, number and distribution of microperoxisomes during the pre and postnatal development of the SMG in order to determine if there were changes in cellular microperoxisomes during the functional differentiation of the various cell types.

Biochemical and cytochemical studies on adenylate cyclase activity in the developing rat submandibular gland: differentiation of the acinar secretory compartment

Development ◽  
1976 ◽  
Vol 36 (2) ◽  
pp. 291-303
Author(s):  
Leslie S. Cutler ◽  
Sevgi B. Rodan
Keyword(s):  
Adenylate Cyclase ◽  
Submandibular Gland ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Apical Plasma Membrane ◽  
Secretory Cells ◽  
Cytochemical Localization ◽  
Membrane Changes ◽  
Rat Submandibular Gland ◽  
Developing Rat

To investigate membrane changes in development of the exocrine cells of the rat submandibular gland (SMG), biochemical and cytochemical studies of adenylate cyclase activity were performed on prenatal and postnatal glands. SMG rudiments and glands were studied from 15 days of gestation up to birth and 1, 2, 3, 4 and 24 weeks after birth. Glands were chemically assayed for adenylate cyclase activity using the procedures of Salomon and coworkers and cytochemically studied using a procedure which was verified biochemically. At 15–16 days of gestation basal adenylate cyclase activity was low and no staining could be observed. Adenylate cyclase activity rose six-fold from the 16th to the 18th day of gestation. Adenylate cyclase staining became evident along the surface of most of the cells of the rudiment at this time. Basal adenylate cyclase activity remained relatively constant from the 18th day of gestation up to 24 weeks of age. However, sequential changes were seen in the cytochemical localization, especially in relation to the apical plasma membrane of the developing secretory cells.

scholarly journals EFFECT OF LOW DOSES OF RADIATION ON MORPHO-FUNCTIONAL STATE OF SUBMANDIBULAR SALIVARY GLAND IN RATS

2019 ◽  
Vol 19 (3) ◽  
pp. 121-126
Author(s):  
O. I. Gryschuk ◽  
S. B. Heraschenko ◽  
O. I. Deltsova ◽  
T. I. Anniuk ◽  
I.O. Mykhailiuk
Keyword(s):  
Salivary Gland ◽  
Submandibular Gland ◽  
Prolonged Exposure ◽  
Transport Processes ◽  
Secretory Cells ◽  
Submandibular Salivary Gland ◽  
Low Doses ◽  
Mucous Cells ◽  
Functional Changes ◽  
Morphometric Investigation

The paper presents the study of the morpho-functional changes in the submandibular gland under the exposure to low doses of radiation in experiment. Material and methods. The experiments were conducted on 80 sexually mature random-bred rats, which were kept in the zone of radiation contamination (zone of permanent radiological control). The external radiation at the moment of the experiment constituted 0.40 and 0.36 mSv due to Cesium-137; internal radiation from separate irradiation sources were 0.16 and 0.11 mSv that are considered to be low doses of radiation. The material for the study (submandibular salivary gland) was taken in terms defined by the experiment from animals euthanized with the overdose of ether anaesthesia. Pieces of the gland were fixed in 10% of the neutral formalin; the sections of the gland were stained with Haematoxylin and Eosin and were studied under the luminous microscope. Microscopic sections were examined for the presence of pathohistological changes in the gland and morphometric investigation of acini and ducts were performed. Results and discussion. During 3-6 months, we observed the progressive morphometrically confirmed degenerative changes in the secretory cells of the acini, the reduction of the diameter of the intercalatated and striated ducts in the serous particles of the gland. We also noted the gradual expansion of the ducts of calibers in the mucous particles of the glands, as the epitheliocytes of their wall acquired more mucus. By the end of the 12th months of the study, the area and height of the cells of the serous terminal divisions and the excretory ducts shrinked (due to atrophy). At the same time, the glandular mucous components (acini and ducts) became predominant. Their terminal branches and excreting ducts develop cystic extensions of the lumen. At the same time transport processes such as releasing secretion from the protein and mucous cells of the terminal secretory sections, became impaired. Conclusion. Studying the dynamic changes in the tissues of the submandibular gland in rats demonstrated the data about pathohistological changes causing the functional insufficiency of the gland in case of prolonged exposure to low doses of radiation. Quantitative and qualitative insufficiency of the mucus formation and salivation can result in the development of sialolithiasis with its all inherent manifestations. In conditions of the exposure to low-dose ionizing radiation, adaptive mechanisms in the epitheliocytes of the duct walls were noticed to start developing, with their transformation into mucous cells and the formation of stone structures in case of mucus thickening.

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