scholarly journals Expression of 20 KD homologous restriction factor of complement on myocardial cells: an immunohistochemical study using acetone-fixed and paraffin-embedded tissues.

1993 ◽  
Vol 41 (1) ◽  
pp. 119-122 ◽  
Author(s):  
T Tada ◽  
H Okada ◽  
N Okada ◽  
H Tateyama ◽  
T Eimoto
Keyword(s):  
Immunohistochemical Study ◽  
Surface Membrane ◽  
Nerve Fibers ◽  
Restriction Factor ◽  
Myocardial Cells ◽  
Paraffin Sections ◽  
Intercalated Discs ◽  
Normal Human ◽  
Cell Sheath ◽  
Complement Deposition

Expression of 20 KD homologous restriction factor of complement (HRF20) (CD59) in normal human heart was immunohistochemically examined with monoclonal antibody (MAb) 1F5. HRF20 was clearly demonstrated on the cell surface membrane and intercalated discs of myocardial cells throughout the ventricular walls. Therefore, this factor may protect normal cardiomyocytes from complement deposition, which has been demonstrated on infarcted cardiomyocytes. Expression of HRF20 was also observed on endothelial cells of the endocardium and on blood vessels, including arteries, capillaries, and veins, and on the Schwann-cell sheath of peripheral nerve fibers. In the present study, we found that the epitopes of HRF20 were well preserved for immunohistochemistry even in acetone-fixed and paraffin-embedded tissues, as well as in frozen tissues that have been conventionally used for HRF20. This method using paraffin sections allows for easier staining and greater accuracy in histological examination.

scholarly journals Immunoistochemical and morphological study of periodont tissues when predicting the results of dental implantation in patients with chronic parodontitis

2019 ◽  
Vol 488 (4) ◽  
pp. 452-456
Author(s):  
A. A. Kulakov ◽  
E. A. Kogan ◽  
T. V. Brailovskaya ◽  
A. P. Vedyaeva ◽  
N. V. Zharkov
Keyword(s):  
Proliferative Activity ◽  
Morphological Study ◽  
Immunohistochemical Study ◽  
Additional Treatment ◽  
Paraffin Sections ◽  
Regenerative Capacity ◽  
Ki 67 ◽  
Dental Implantation ◽  
Growth Zones ◽  
Severe Degree

A morphological and immunohistochemical study of 24 gums biopsies was conducted in 19 patients aged 35-60 years with a diagnosis of partial secondary edentulous, chronic generalized periodontitis of moderate and severe degree (14 patients), and also without pathological changes in the periodontal disease (5 patients), who underwent dental implantation. Immunohistochemical reactions with antibodies to Ki-67, VEGF, SMA were performed on serial paraffin sections. It has been established that chronic periodontitis is characterized by a higher proliferative activity of the epithelium, which reflects its hyperplastic changes, as well as a lower content of SMA positive cells and the practical absence of the formation of privascular couplings from SMA-positive cells that are associated in tissues with growth zones, which indirectly indicates reduced tissue regenerative capacity. Therefore, in the case of the operation of dental implantation requires additional treatment aimed at anti-inflammatory and pro-regenerative effects.

scholarly journals C4d Deposition in Fetal Vessels of the Placenta in Neonatal Lupus Syndrome

2019 ◽  
Vol 2019 ◽  
pp. 1-4
Author(s):  
Yuichiro Sato ◽  
Tomoko Yamaguchi ◽  
Jyunsuke Muraoka ◽  
Hajime Taniguchi ◽  
Atsushi Kisanuki ◽  
...  
Keyword(s):  
Immunohistochemical Study ◽  
Neonatal Lupus ◽  
Chorionic Villi ◽  
Autoimmune Syndrome ◽  
Emergency Cesarean ◽  
Upper And Lower Extremities ◽  
The Face ◽  
Lupus Syndrome ◽  
Complement Deposition ◽  
Fetal Vessels

Neonatal lupus syndrome (NLS) is a rare, passively acquired autoimmune syndrome caused by maternal autoantibodies. We describe a case of a newborn with NLS and the accompanying placental findings. A female neonate was born by emergency cesarean delivery due to non-reassuring fetal status at 35 weeks and 3 days. This neonate had congenital erythematous and scar lesions on the face, back, and upper and lower extremities. Maternal and fetal anti-SSA and SSB antibodies were elevated and this baby was diagnosed as NLS. Histologically, the chorionic villi demonstrated capillary shrinkage. An immunohistochemical study revealed complement deposition (C4d) in the capillaries of the villi and umbilical vessels. Our findings suggest that maternal autoantibodies affect the inflammatory response of the fetus through the placenta and that C4d deposition may be useful for diagnosing NLS.

Protein phosphatase 2Cα expression in normal human tissues: an immunohistochemical study

2001 ◽  
Vol 116 (1) ◽  
pp. 31-39 ◽  
Author(s):  
Beatriz Lifschitz-Mercer ◽  
Yuri Sheinin ◽  
Daniella Ben-Meir ◽  
Letizia Bramante-Schreiber ◽  
Leonor Leider-Trejo ◽  
...  
Keyword(s):  
Protein Phosphatase ◽  
Immunohistochemical Study ◽  
Human Tissues ◽  
Normal Human
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