scholarly journals Immunoistochemical and morphological study of periodont tissues when predicting the results of dental implantation in patients with chronic parodontitis

2019 ◽  
Vol 488 (4) ◽  
pp. 452-456
Author(s):  
A. A. Kulakov ◽  
E. A. Kogan ◽  
T. V. Brailovskaya ◽  
A. P. Vedyaeva ◽  
N. V. Zharkov
Keyword(s):  
Proliferative Activity ◽  
Morphological Study ◽  
Immunohistochemical Study ◽  
Additional Treatment ◽  
Paraffin Sections ◽  
Regenerative Capacity ◽  
Ki 67 ◽  
Dental Implantation ◽  
Growth Zones ◽  
Severe Degree

A morphological and immunohistochemical study of 24 gums biopsies was conducted in 19 patients aged 35-60 years with a diagnosis of partial secondary edentulous, chronic generalized periodontitis of moderate and severe degree (14 patients), and also without pathological changes in the periodontal disease (5 patients), who underwent dental implantation. Immunohistochemical reactions with antibodies to Ki-67, VEGF, SMA were performed on serial paraffin sections. It has been established that chronic periodontitis is characterized by a higher proliferative activity of the epithelium, which reflects its hyperplastic changes, as well as a lower content of SMA positive cells and the practical absence of the formation of privascular couplings from SMA-positive cells that are associated in tissues with growth zones, which indirectly indicates reduced tissue regenerative capacity. Therefore, in the case of the operation of dental implantation requires additional treatment aimed at anti-inflammatory and pro-regenerative effects.

scholarly journals Immunohistochemical Study of Tumor Cells Proliferative Activity at Different Graduations of Prostate Cancer

2019 ◽  
Vol 23 (4) ◽  
pp. 364-372 ◽  
Author(s):  
G. Y. Kudryavtsev ◽  
L. V. Kudryavtseva ◽  
L. M. Mikhaleva ◽  
I. I. Babichenko
Keyword(s):  
Prostate Cancer ◽  
Prostate Specific Antigen ◽  
Proliferative Activity ◽  
Immunohistochemical Study ◽  
Activity Index ◽  
Clinical Stage ◽  
Prostate Gland ◽  
Specific Antigen ◽  
Ki 67 ◽  
Degree Of Differentiation

Prostate cancer is one of the leading causes of men death in developed countries. Modern diagnostic methods, including a puncture biopsy of the prostate gland, make it possible to verify oncology in the early stages, however, routine studies do not always allow to predict the course of the disease and outcome. The aim of our study was to analyze the clinical, morphological and immunohistochemical features of the proliferative activity of adenocarcinoma cells using the Ki-67 marker, to compare the degree of proliferative activity in tumors of various degrees of malignancy (according to Gleason’s classification), as well as to compare this indicator with the clinical stage of the disease, the level of prostate-specific antigen in the blood, the size of the prostate gland. Materials and Methods: On the basis of the City Clinical Hospital No. 31 and the Veteran Hospital No. 2, paraffin blocks with material obtained as a result of prostate biopsy, transurethral resection, and radical prostatectomy were selected. A morphological and immunohistochemical study of the material with the Ki-67 marker and a quantitative assessment of the degree of proliferative activity were carried out. Data were analyzed using the STATISTICA 10.0 program using estimates of the normality of the data distribution according to the Shapiro-Wilk W-test, the significance of differences was estimated using the Mann-Whitney U-test, and correlation relationships using Spearman’s correlation coefficient. Results: Statistically significant differences in the degree of proliferative activity in groups differing in the degree of differentiation were revealed. A statistically significant direct correlation of moderate severity was revealed when comparing proliferative activity with the degree of differentiation according to the Gleason system (rs = 0.523) and the clinical stage of the disease (rs = 0.646). No statistically significant correlation was found between indicators such as prostate-specific antigen level, age, prostate volume, and proliferative activity index. Conclusion: taking into account the proliferative activity index in addition to clinical and morphological studies helps to diagnose and subsequently predict the course of prostate cancer.

scholarly journals Immunohistochemical study of P53 protein expression in different prostate cancer Gleason grading groups

2020 ◽  
Vol 24 (2) ◽  
pp. 145-155 ◽  
Author(s):  
G. Y. Kudryavtsev ◽  
L. V. Kudryavtseva ◽  
L. M. Mikhaleva ◽  
Y. Y. Kudryavtseva ◽  
N. A. Solovyeva ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Proliferative Activity ◽  
Immunohistochemical Study ◽  
P53 Protein ◽  
Public Health Problem ◽  
Developed Countries ◽  
Ki 67 ◽  
P53 Expression ◽  
Gleason Grading ◽  
The Difference

Prostate cancer (PC) remains an urgent public health problem, especially in developed countries. The use of immunohistochemical research methods in addition to the morphological classification of prostate adenocarcinomas allows a more accurate diagnosis and prognosis of the disease. The aim of the study is to identify isoforms of P53 using clones of mouse antibodies (D-07 and Y5; Epitomics, USA) in prostate cancer with different proliferative activity and the degree of malignancy. Materials and Methods: The work included surgical material for prostate resection and prostatectomy, as well as biopsy specimens (56 cases in total). An immunohistochemical study was carried out with the Ki-67 marker, as well as with mouse monoclonal antibodies (D-07 and Y5) to the P53 protein, interacting with its wild and mutant isoforms. The significance of the difference in the samples was determined using the Mann-Whitney U-test, correlation relationships were determined using the Spearman coefficient. Results: Expression of P53 upon interaction with antibodies D-07 and Y5 was determined in 56.3% and 39.6%, respectively. A statistically significant direct correlation was found between the severity of P53 expression when interacting with Y5 antibodies and the degree of tumor differentiation (rs = 0.567, p 0.05), as well as between the expression level of this protein and tumor proliferative activity (rs = 0.698, p 0.05). Conclusion: Antibodies of clone D-07, interacting with both wild and mutant isoforms of P53 protein, show positive expression in adenocarcinomas of all degrees. Expression of the mutant P53 protein is most pronounced in low-differentiated carcinomas and correlates with high proliferative activity of tumor cells, which may be associated with a loss in the induction of P53-dependent apoptosis.

scholarly journals Proliferative activity of intrinsic cell populations in the normal human kidney.

1994 ◽  
Vol 4 (12) ◽  
pp. 2032-2039 ◽  
Author(s):  
T Nadasdy ◽  
Z Laszik ◽  
K E Blick ◽  
L D Johnson ◽  
F G Silva
Keyword(s):  
Renal Cell ◽  
Proliferative Activity ◽  
Human Kidney ◽  
Capillary Endothelium ◽  
Cell Populations ◽  
Tubular Epithelium ◽  
Paraffin Sections ◽  
Ki 67 ◽  
Collecting Ducts ◽  
Normal Human

The proliferative activity of various normal human renal cell populations is unknown. Recently, antibodies to cell proliferation-associated nuclear proteins, such as proliferating cell nuclear antigen (PCNA) and KI-67, which are applicable to archival paraffin sections, became available. With antibodies to PCNA and Ki-67 after microwave pretreatment of the paraffin sections, the proliferation indexes (ratio of positive nuclei with PCNA and Ki-67 antibodies/all nuclei counted x 100, i.e. percentage of positive cells) of 12 different intrinsic renal cell populations in 20 normal human kidneys have been determined. The following proliferation indexes (percentages of positive cells) were found with the PCNA and the Ki-67 antibodies, respectively: proximal tubular epithelium, 0.22, 0.24; thin limb of Henle, 0.29, 0.30; thick ascending limb of Henle, 0.32, 0.29; distal tubular epithelium (distal convoluted tubules and cortical collecting ducts, 0.33, 0.44; medullary collecting ducts, 0.32, 0.3; glomerular mesangial cells, 0.07, 0.12; glomerular visceral epithelial cells, 0.04, 0.08; glomerular parietal epithelial cells, 0.07, 0.1; glomerular capillary endothelium, 0.42, 0.47; peritubular capillary endothelial cells, 0.38, 0.43; endothelium of large intrarenal vessels (arteries and veins), 0.09, 0.12. Thus, normally capillary endothelium (glomerular and peritubular) appears to have the highest proliferation index in the human kidney by these techniques. These results indicate major variation in the proliferative activity of normal human renal cell populations, along with a significant correlation between PCNA and Ki-67 staining. Furthermore, this study provides normal values for the proliferative activity of different human renal cell populations.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Lateral differences of breast cancer proliferative activity (KI-67)

2016 ◽  
Vol 97 (4) ◽  
pp. 550-555
Author(s):  
A P Dmitrenko
Keyword(s):  
Breast Cancer ◽  
Proliferative Activity ◽  
Immunohistochemical Study ◽  
Progesterone Receptors ◽  
Ki 67 ◽  
Primary Tumors ◽  
Study Protocols ◽  
Tumor Lesion ◽  
Significant Difference ◽  
The Right

Aim. To determine lateral differences of breast cancer proliferative activity Ki-67.Methods. According to immunohistochemical study protocols analysis of material of 500 patients with breast cancer was conducted. In primary tumors estrogen and progesterone receptors expression, Ki-67, C-erbB-2 was studied.Results.Using two-way analysis of variance, it was found that Ki-67 index was significantly influenced by both side of the tumor lesion (p=0.009) and age of patients (p=0.0002). A higher Ki-67 corresponded to right-sided cancer localization. Statistically significant age differences of Ki-67 index are marked only in right-sided cancer (pConclusion. Statistically significant difference of Ki-67 index in right- and left-sided breast cancer was found, significantly higher Ki-67 was detected in the right-sided tumors, Ki-67 are present only in patients before 60 years.

scholarly journals Müllerian adenosarcoma of the uterus with sarcomatous overgrowth following tamoxifen treatment for breast cancer

2000 ◽  
Vol 55 (1) ◽  
pp. 17-20 ◽  
Author(s):  
Filomena Marino Carvalho ◽  
Jesus Paula Carvalho ◽  
Eduardo Vieira da Motta ◽  
Jorge Souen
Keyword(s):  
Breast Cancer ◽  
Breast Carcinoma ◽  
Estrogen Receptors ◽  
Stromal Cells ◽  
Proliferative Activity ◽  
Immunohistochemical Study ◽  
Tamoxifen Treatment ◽  
Ki 67 ◽  
Total Hysterectomy ◽  
Mesenchymal Neoplasms

Müllerian adenosarcoma with sarcomatous overgrowth presented by a 52-year-old female patient after adjuvant tamoxifen treatment for breast carcinoma is described. The diagnosis was made on histological basis after curettage and complementary total hysterectomy with bilateral salpingo-oophorectomy. The immunohistochemical study showed high expression of estrogen receptors in the epithelial component of the lesion and irregularly positive findings in the stroma. The proliferative activity evaluated by Ki-67 immunoexpression was higher in the stroma than the epithelium. Some of the stromal cells showed rhabdomyoblastic differentiation. The association of tamoxifen use and development of mesenchymal neoplasms is discussed.

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