S‐100‐positive nerve fibers in hepatocellular carcinoma and intrahepatic cholangiocarcinoma: An immunohistochemical study

AIM: To explore the diagnostic ability of contrast-enhanced ultrasound (CEUS) in distinguishing intrahepatic cholangiocarcinoma (ICC) from hepatocellular carcinoma (HCC). MATERIALS AND METHODS: PubMed, EMBASE, Cochrane Library, and Web of Science were systematically searched for studies reporting the diagnostic accuracy of CEUS in differentiating ICC from HCC. The diagnostic ability of CEUS was assessed based on the pooled sensitivity, specificity, diagnostic odds ratio (DOR), positive likelihood ratio (PLR), negative likelihood ratio (NLR) and area under the curve (AUC) with 95% confidence intervals (CIs). The methodologic quality was assessed by the QUADAS-2 tool. Subgroup analyses, meta-regression and investigation of publication bias were performed to identify the source of heterogeneity. RESULTS: A total of eight studies were included, consisting of 1,116 patients with HCC and 529 with ICC. The general diagnostic performance of CEUS in distinguishing ICC and HCC were as follows: pooled sensitivity, 0.92 (95% CI: 0.84–0.96); pooled specificity, 0.87 (95% CI: 0.79–0.92); pooled PLR, 7.1 (95% CI: 4.1–12.0); pooled NLR, 0.09 (95% CI: 0.05–0.19); pooled DOR, 76 (95% CI: 26–220) and AUC, 0.95(95% CI: 0.93–0.97). Different liver background may be a potential factor that influenced the diagnostic accuracy of CEUS according to the subgroup analysis, with the pooled DOR of 89.67 in the mixed liver background group and 46.87 in the cirrhosis group, respectively. Six informative CEUS features that may help differentiate HCC from ICC were extracted. The three CEUS features favoring HCC were arterial phase hyperenhancement(APHE), mild washout and late washout (>60s); the three CEUS favoring ICC were arterial rim enhancement, marked washout and early washout(<60s). No potential publication bias was observed. CONCLUSION: CEUS showed great diagnostic ability in differentiating ICC from HCC, which may be promising for noninvasive evaluation of these diseases.

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The Presence of Small Nerve Fibers in the Tumor Microenvironment as Predictive Biomarker of Oncological Outcome Following Partial Hepatectomy for Intrahepatic Cholangiocarcinoma

Cancers ◽

10.3390/cancers13153661 ◽

2021 ◽

Vol 13 (15) ◽

pp. 3661

Author(s):

Jan Bednarsch ◽

Xiuxiang Tan ◽

Zoltan Czigany ◽

Dong Liu ◽

Sven Arke Lang ◽

...

Keyword(s):

Tumor Microenvironment ◽

Intrahepatic Cholangiocarcinoma ◽

Cox Regression ◽

Oncological Outcome ◽

Nerve Fibers ◽

Nodal Metastases ◽

Cancer Specific Survival ◽

Curative Intent ◽

Term Outcome ◽

Long Term Outcome

The oncological role of the density of nerve fibers (NFs) in the tumor microenvironment (TME) in intrahepatic cholangiocarcinoma (iCCA) remains to be determined. Therefore, data of 95 iCCA patients who underwent hepatectomy between 2010 and 2019 was analyzed regarding NFs and long-term outcome. Extensive group comparisons were carried out and the association of cancer-specific survival (CSS) and recurrence-free survival (RFS) with NFs were assessed using Cox regression models. Patients with iCCA and NFs showed a median CSS of 51 months (5-year-CSS = 47%) compared to 27 months (5-year-CSS = 21%) in patients without NFs (p = 0.043 log rank). Further, NFs (hazard ratio (HR) = 0.39, p = 0.002) and N-category (HR = 2.36, p = 0.010) were identified as independent predictors of CSS. Patients with NFs and without nodal metastases displayed a mean CSS of 89 months (5-year-CSS = 62%), while patients without NFs or with nodal metastases but not both showed a median CCS of 27 months (5-year-CSS = 25%) and patients with both positive lymph nodes and without NFs showed a median CCS of 10 months (5-year-CSS = 0%, p = 0.001 log rank). NFs in the TME are, therefore, a novel and important prognostic biomarker in iCCA patients. NFs alone and in combination with nodal status is suitable to identify iCCA patients at risk of poor oncological outcomes following curative-intent surgery.

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Melatonin administration provokes the activity of dendritic reticular cells in the seminal vesicle of Soay ram during the non-breeding season

Scientific Reports ◽

10.1038/s41598-020-79529-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Hanan H. Abd-Elhafeez ◽

A. H. S. Hassan ◽

Manal T. Hussein

Keyword(s):

Estrogen Receptor Alpha ◽

Close Contact ◽

Secretory Activity ◽

Treated Group ◽

Nerve Fibers ◽

Stimulatory Action ◽

Transmission Electron ◽

Melatonin Administration ◽

S 100 ◽

Lysosomal System

AbstractDendritic cells (DCs) are innate immune cells which engulf, process and present antigens to the naïve T-lymphocyte cells. However, little is known about the effect of melatonin on the DCs. The present study aimed to investigate the morphology and distribution of the DCs by transmission electron microscopy and Immunohistochemistry after melatonin administration. A total of 8 out of 15 adult ram was randomly selected to receive the melatonin implant and the remaining 7 animals received melatonin free implants. DCs showed positive immunoreactivity for CD117, S-100 protein and CD34. There is an obvious increase in the number of the positive immunoreactive cells to CD3, estrogen receptor alpha and progesterone in the treated groups. The expression of CD56 and MHCII in the DCs was abundant in the treated groups. The ultrastructure study revealed that melatonin exerts a stimulatory effect on the DCs which was associated with increment in the secretory activity of DCs. The secretory activity demarcated by an obvious increase in the number of mitochondria, cisternae of rER and a well-developed Golgi apparatus. The endosomal- lysosomal system was more developed in the treated groups. A rod-shaped Birbeck granule was demonstrated in the cytoplasm of the melatonin treated group. DCs were observed in a close contact to telocytes, T-Lymphocytes, nerve fibers and blood vessels. Taken together, melatonin administration elicits a stimulatory action on the DCs and macrophages through increasing the size, the number and the endosomal compartments which may correlate to increased immunity.

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Mixed hepatocellular cholangiocarcinoma and intrahepatic cholangiocarcinoma in patients undergoing transplantation for hepatocellular carcinoma

Liver Transplantation ◽

10.1002/lt.22307 ◽

2011 ◽

Vol 17 (8) ◽

pp. 934-942 ◽

Cited By ~ 101

Author(s):

Gonzalo Sapisochin ◽

Nicholas Fidelman ◽

John P. Roberts ◽

Francis Y. Yao

Keyword(s):

Hepatocellular Carcinoma ◽

Intrahepatic Cholangiocarcinoma

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An immunohistochemical study on the distribution of histiocytes containing S-100 protein-like antigen in cutaneous T-cell lymphoma/leukemia; a preliminary report

Cellular and Molecular Life Sciences ◽

10.1007/bf01954945 ◽

1982 ◽

Vol 38 (11) ◽

pp. 1349-1351 ◽

Cited By ~ 2

Author(s):

K. Takahashi ◽

T. Akagi ◽

Y. Ohtsuki ◽

H. Sonobe ◽

H. Yamaguchi

Keyword(s):

T Cell ◽

Preliminary Report ◽

Immunohistochemical Study ◽

Cell Lymphoma ◽

T Cell Lymphoma ◽

Cutaneous T Cell Lymphoma ◽

S 100

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Cytokeratin expression in human hepatocellular carcinoma : An Immunohistochemical study

Journal of Hepatology ◽

10.1016/s0168-8278(87)80227-1 ◽

1987 ◽

Vol 5 ◽

pp. S70

Keyword(s):

Hepatocellular Carcinoma ◽

Immunohistochemical Study ◽

Human Hepatocellular Carcinoma ◽

Cytokeratin Expression

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Fibrous papule: An immunohistochemical study with an antibody to S-100 protein

Journal of the American Academy of Dermatology ◽

10.1016/s0190-9622(83)70142-8 ◽

1983 ◽

Vol 9 (3) ◽

pp. 360-362 ◽

Cited By ~ 22

Author(s):

Joan Spiegel ◽

Mehrdad Nadji ◽

Neal S. Penneys

Keyword(s):

Immunohistochemical Study ◽

S 100

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Immunohistochemical study of a canine neurofibroma

Journal of the South African Veterinary Association ◽

10.4102/jsava.v83i1.18 ◽

2012 ◽

Vol 83 (1) ◽

Cited By ~ 3

Author(s):

Hamidreza Fattahian ◽

Pejman Mortazavi ◽

Hamidreza Moosavian ◽

Hamid Mohyeddin ◽

Roozbeh Moridpour

Keyword(s):

Case Report ◽

Diagnostic Imaging ◽

Peripheral Nerve ◽

Schwann Cells ◽

Immunohistochemical Study ◽

Collagen Fibres ◽

Spindle Cells ◽

Left Mandible ◽

S 100 ◽

Diagnostic Approaches

Accurate diagnostic approaches to differentiate peripheral nerve sheet tumours from others have not been firmly established. The aim of this case report was to diagnose neurofibroma using a combination of diagnostic imaging, histopathology and immunohistochemistry, which were applied to a canine neurofibroma arising in the left mandible. The tumour was surgically excised and examined histologically. Round or spindle cells, with elongated, dense and homogenous chromatin and pale cytoplasm typical of Schwann cells in an abundant fibromyxomatous stroma, with ruby collagen fibres were seen. Immunohistochemistry demonstrated that S-100 and vimentin were more than 70% positive. Neurofibroma may therefore be recognisable using markers such as S-100 and vimentin.

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Rare Lipomatous Tumors with Osseous and/or Chondroid Differentiation in the Oral Cavity Report of Two Cases and Review of the Literature

International Journal of Dentistry ◽

10.1155/2009/143460 ◽

2009 ◽

Vol 2009 ◽

pp. 1-6 ◽

Cited By ~ 9

Author(s):

Kayo Kuyama ◽

Sisilia Fusi Fifita ◽

Masamichi Komiya ◽

Yan Sun ◽

Yoshiaki Akimoto ◽

...

Keyword(s):

Oral Cavity ◽

Immunohistochemical Study ◽

Fat Cells ◽

Fatty Tissue ◽

Review Of The Literature ◽

Woven Bone ◽

Osteoid Tissue ◽

Spindle Cells ◽

S 100 ◽

Lipomatous Tumors

The purpose of this study was to determine the clinicopathological and immunohistochemical features of lipoma/fibrolipoma with rare occasions as osseous and/or chondroid differentiation in the oral cavity. Two cases of the tumors, who presented with a painless, relatively hard mass on the oral mucosa, were studied. These were consisted of a well-circumscribed mass of fatty tissue with chondroid and significant fibrous component intermixed with the lobules of fat cells with chondroid and woven bone component, respectively. Immunohistochemical study revealed that peripheral spindle cells around chondroid tissue stained diffusely for S-100 & and Sox-9, though peripheral spindle cells around osteoid tissue only stained for RUNX-2. According to review of the literature, lipoma/fibrolipoma with osseous and/or chondroid differentiation was 18 cases. Also fibrolipoma with osseous and chondroid differentiation is the first to be reported here. These results indicated that the cartilage/bone is produced by differentiation of undifferentiated mesenchymal cells of stroma.

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