scholarly journals Ultrastructural detection of calcium and magnesium in the chromatoid body of mouse spermatids by electron spectroscopic imaging and electron energy loss spectroscopy.

1993 ◽  
Vol 41 (8) ◽  
pp. 1155-1162 ◽  
Author(s):  
V B Rouelle-Rossier ◽  
M Biggiogera ◽  
S Fakan
Keyword(s):  
Energy Loss ◽  
Electron Energy ◽  
Spectroscopic Imaging ◽  
Ultrastructural Level ◽  
Electron Spectroscopic Imaging ◽  
Chromatoid Body ◽  
In Situ Immobilization ◽  
Calcium And Magnesium ◽  
Electron Energy Loss

We studied at the ultrastructural level the presence of calcium and magnesium in the chromatoid body (CB) in mouse spermatids. In addition, the presence of these two cations was also examined in the nucleolus. By electron spectroscopic imaging (ESI) and electron energy loss spectroscopic (EELS) analyses on glutaraldehyde/pyroantimonate-fixed material, we showed the association of pyroantimonate precipitate granules containing calcium and/or magnesium with the CB. The granules in both active (primary spermatocytes) and inactive (spermatids) nucleoli contain mainly calcium. Our results confirm that although the pyroantimonate technique used alone suffers from a lack of specificity, it can be useful for in situ immobilization of different cations that are further resolved with methods of elemental analysis. Without pyroantimonate fixation, only very few spots containing calcium can be localized by ESI and EELS.

scholarly journals Ultrastructural distribution of terbium across capillary endothelium: detection by electron spectroscopic imaging and electron energy loss spectroscopy.

1990 ◽  
Vol 38 (2) ◽  
pp. 275-282 ◽  
Author(s):  
R C Wagner ◽  
S C Chen
Keyword(s):  
Energy Loss ◽  
Electron Energy ◽  
Electron Energy Loss Spectroscopy ◽  
Spectroscopic Imaging ◽  
Capillary Endothelium ◽  
Electron Spectroscopic Imaging ◽  
Thin Sections ◽  
Rete Mirabile ◽  
Heavy Electron ◽  
Electron Energy Loss

We used terbium as an intravital tracer of permeability pathways across the walls of capillaries in the rete mirabile of the eel swimbladder and in frog mesentery. Terbium was detected in unstained ultra-thin sections by electron density using electron spectroscopic imaging (ESI) and by electron energy loss spectroscopy (EELS). Enhancement of intrinsic contrast in zero loss images (elastically scattered electrons) permitted imaging of membrane-bound compartments and terbium within them which might otherwise have been undetected in counterstained sections. Element-selective imaging with EELS indicated that terbium was associated with heavy electron-dense deposits, but the terbium mass:volume of sections in areas of lighter deposition was insufficient to obtain a terbium signal. In the rete capillaries, terbium was deposited on the luminal surface, throughout vesicular profiles, and in the interstitium, but could not be traced through interendothelial junctions. Fine terbium deposits were detectable throughout apparent vesicular connections across the endothelium. In the frog mesentery, terbium penetrated some but not all interendothelial clefts, and was detectable in small quantities within luminal and abluminal vesicular profiles and in the interstitium. The results indicate that in the rete capillaries, terbium permeates the capillary via a transcellular route. This route may be provided by transient fusions of luminal and abluminal vesicular compartments.

scholarly journals Electron spectroscopic imaging (ESI) and electron energy loss spectroscopy (EELS) of multilamellar bodies and multilamellar body-like structures in tannic acid-treated alveolar septal cells.

1994 ◽  
Vol 42 (6) ◽  
pp. 805-809 ◽  
Author(s):  
M Ochs ◽  
H Fehrenbach ◽  
J Richter
Keyword(s):  
Energy Loss ◽  
Electron Energy ◽  
Tannic Acid ◽  
Electron Energy Loss Spectroscopy ◽  
Spectroscopic Imaging ◽  
Structural Similarity ◽  
Alveolar Epithelial Cells ◽  
Electron Spectroscopic Imaging ◽  
Multilamellar Bodies ◽  
Electron Energy Loss

We used electron spectroscopic imaging (ESI) and electron energy loss spectroscopy (EELS) to compare multilamellar bodies (MLB) of Type II alveolar epithelial cells with MLB-like structures that are present in various alveolar septal cells after fixation with tannic acid. Despite their structural similarity in conventional transmission electron microscopy, the phosphorus signal recorded by both ESI and EELS was considerably higher in multilamellar bodies than in MLB-like structures. This indicates that they are different in chemical composition.

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