HISTOCHEMICAL DEMONSTRATION OF ACID PHOSPHATASE ACTIVITY IN OSTEOCLASTS
1959 ◽
Vol 7
(1)
◽
pp. 39-41
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Keyword(s):
Azo Dye
◽
High acid phosphatase activity was observed in osteoclasts of several species using a reproducible azo-dye technique. High activity of two distinct enzymes, acid and alkaline phosphatase, are associated with osteoclasts and osteoblasts respectivey. Althouth frozen-dried tissues are recommended for definitive studies, the enzyme techniques used give satisfactory results with cold acetone-fixed tissues. The most accurate localization of acid phosphatase in osteoclasts in controlled comparative studies is obtained with double-embedded frozen-dried undecalcified tissues in conjunction with naphthol AS-phosphates.
1966 ◽
Vol 14
(2)
◽
pp. 171-176
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1969 ◽
Vol 17
(12)
◽
pp. 799-806
◽
1978 ◽
Vol 58
(3)
◽
pp. 727-730
◽
2002 ◽
Vol 6
(1)
◽
1966 ◽
Vol 89
(1)
◽
pp. 27-34
◽
1993 ◽
Vol 38
(7)
◽
pp. 611-617
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