Nitrogen species specific phosphorus mineralization in temperate floodplain soils

As an essential component of enzymes, higher N availability from agricultural runoff to forest soils may boost the activity of phosphatase, increasing the bioavailability of phosphate. The objective of this study was to evaluate P mineralization rates in temperate floodplain soils as a function of inorganic N species (i.e., ammonium and nitrate) and amendment rate (1.5–3.5 g N kg−1). Accordingly, the soil was amended with nitrate and ammonium, and P dynamics were monitored during a 40-day incubation. The addition of ammonium significantly boosted acid and alkaline phosphatase activity by 1.39 and 1.44 µmol p-nitrophenol P (pNP) g−1 h−1, respectively. The degree of increase was positively correlated with the amendment rate. Likewise, the P mineralization rate increased by 0.27 mg P kg−1 in the 3.5 g N kg−1 ammonium treatment. 31P nuclear magnetic resonance spectroscopic analysis further supported the reduction in organic orthophosphate diesters on day 30. Meanwhile, the addition of nitrate promoted P mineralization to a lesser degree but did not increase phosphatase activity. While floodplain soils have great potential to sequester anthropogenic P, high availability of inorganic N, especially ammonium, could promote P mineralization, potentially increasing P fertility and/or reducing P the sequestration capacity of floodplain soils.

Analysis of oral health and salivary biochemical parameters of women with anorexia and bulimia nervosa

Análisis de parámetros bioquímicos de salud bucal y salival de mujeres con anorexia y bulimia nerviosa Objective: The aim of this study was to analyze the association between caries, dental erosion and salivary biochemical profile of women with anorexia and bulimia, comparing them with a control group. Methods: The participants were women from the Eating Disorders Program of the Mental Health Clinic. We evaluated caries and dental erosion and collected stimulated saliva samples for the analyses of salivary flow, phosphor, calcium, acid and alkaline phosphatase, amylase, FRAP and uric acid. Data were expressed as means and standard deviations and were analysed using Mann-Whitney test with p<0.05 regarded as significant. Results: 13 women with anorexia and bulimia participated in the study, with an mean age of 28.9 years, and 15 comprised the control group. The DMFT index was 15.5 for the case group and 4.0 for the control group. Erosion was found mostly on the palatal/lingual surface and on incisor teeth of case group. Most biochemical analyzes showed a significant difference between the two groups. Conclusion: We concluded that, in patients with anorexia and bulimia, due to a decreased salivary flow and self-induced vomiting habits, there is an increase in the caries and dental erosion index, as well as salivary changes.

Evaluation of impacts of potato crops and livestock farming in Neotropical high Andean Páramo soils, Colombia

Páramos are Neotropical ecosystems that result from insular formations dominated by tufted hair grasses and shrubs, and are located above the continuous forest track and below the perpetual snowline of the highest peaks in the Northern Andes. The aim of this research was to check the reliability of the hypothesis that the potato cultivation and livestock farming activities modify the quality of Páramo soils. For this, soil samples were taken from soil used with three different purposes at two different heights: Páramo with the least possible intervention, and Páramo where the activities of potato cropping and livestock farming were carried out; from these, and that have been determined to have physicochemical properties, abundance of functional groups of microorganisms (nitrogen-fixing, phosphate solubilizers, cellulolytic microorganisms), and enzymatic activities (urease, protease, β-glucosidase, acid, and alkaline phosphatase). The gravimetric humidity, apparent density, organic carbon, cation exchange capacity, urease, acid phosphatase, and β-glucosidase showed statistically significant differences (P < 0.05), indicating that there is a change in the quality of the soil that is exposed to agricultural activities. As for microorganisms the hypothesis was not confirmed; there are warning signs for the future in the case of continuing with these practices, but there are no significant differences.

Effects of Cowpea-Amaranth Intercropping and Fertiliser Application on Soil Phosphatase Activities, Available Soil Phosphorus, and Crop Growth Response

Low available soil phosphorus (P) is associated with its immobility, which renders it unavailable for plant uptake. In addition, farmers normally apply inorganic fertilisers to legumes to activate soil-bound phosphorus using root exudates. Sufficient soil mineral nutrition is key to sustainable crop production, and hence food and nutritional security. The aim of this study was to quantify the acid and alkaline phosphatase activity as an indicator of P supply and availability under varying levels of nitrogen, phosphorus and potassium (NPK) fertilization and different cropping systems. An intercropping (cowpea and amaranth) and fertiliser (control, 25%, 50%, and 100% of the recommended NPK levels) field trial was laid out in a 2 × 4 factorial treatment structure in a completely randomized design (CRD) with four replications. There was higher acid and alkaline phosphatase activity in the rhizosphere of cowpea and amaranth grown as sole crops compared to those from intercropping. The cowpea and amaranth plants grown without fertiliser or 25% NPK had the highest rhizospheric phosphatase activity, while 100% NPK application exhibited the least. The markedly higher phosphatase activity from the low fertiliser application treatments indicates the possible stimulation of microbial activity to supplement P demands for the crops. The study revealed that the application of lower rates inorganic fertilisers in a legume intercrop stimulates the activity of the phosphatase enzymes, which can subsequently liberate soil-bound phosphorus. Plant tissue phosphorus concentration of cowpea and amaranth plants increased proportionately to the increase in fertiliser application up to 50% of the recommended NPK level. The land equivalent ratio (LER) was greater than 1, indicating that it is more beneficial to intercrop cowpea and amaranth as opposed to growing them as sole crops. Overall, the application of NPK fertilizer to amounts of up to 50%, based on the results of this study, appear to be better than 100% in terms of biomass accumulation and phosphate activity.