New microbiological strategies that enable the selective recovery and recycling of metals from acid mine drainage and mine process waters

2012 ◽  
Vol 76 (7) ◽  
pp. 2683-2692 ◽  
Author(s):  
I. Ňancucheo ◽  
S. Hedrich ◽  
D. B. Johnson
Keyword(s):  
Acid Mine Drainage ◽  
Mine Drainage ◽  
Environmental Pollutants ◽  
Microbial Oxidation ◽  
Mine Waters ◽  
Acid Mine ◽  
Acidophilic Bacteria ◽  
Active Chemical ◽  
Valuable Metals ◽  
Commercial Value

AbstractApproaches currently used for remediating acid mine drainage (chiefly active chemical treatment and passive bioremediation systems) have a number of major detractions, including their failure to recover potentially valuable metals from these waters. Bioremediation strategies that utilize reactor-housed microorganisms can circumvent this problem, but have tended not to be widely used due to their relatively high costs. We have devised innovative approaches for remediating mine waters that use acidophilic bacteria to remove metals either as oxidized or reduced phases, using modular bioreactors that are designed to operate at minimal cost and to generate products that have commercial value. A composite system is described that combines microbial oxidation of ferrous iron with abiotic precipitation of ferric iron as schwertmannite, a mineral that has commercial value as an absorbent of arsenate and other environmental pollutants, and as a pigment. Sulfidogenic bioreactors maintained at acidic pH values are used to selectively precipitate metal sulfides, such as CuS. Tests with synthetic mine drainage containing mixtures of soluble metals confirmed that these systems can generate relatively pure mineral deposits from complex acid waters. The units are designed to be configured differently, according to the nature of the mine water requiring treatment.

Molecular Identification of Iron Oxidizing Bacteria Isolated from Acid Mine Drainages in Peru

2013 ◽  
Vol 825 ◽  
pp. 84-87 ◽  
Author(s):  
Michel Abanto ◽  
Nicolaza Pariona ◽  
Julio Calderon ◽  
Gregory Guerra ◽  
Rina Ramirez ◽  
...  
Keyword(s):  
Acid Mine Drainage ◽  
16S Rrna ◽  
Mine Drainage ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Acid Mine ◽  
The North ◽  
Acidophilic Bacteria ◽  
Iron Oxidizing Bacteria ◽  
Acid Mine Drainage Generation

Acidophilic iron-oxidizing microorganisms are important in both environmental and biotechnological applications. These microorganisms are known to accelerate the dissolution of sulfur minerals such as pyrite (FeS2), leading to the acid mine drainage generation , a serious pollution problem, that makes these microorganisms essential to the commercial processing of minerals and sulfur. In order to answer this question, diversity of native acidophilic bacteria isolated from acid mine drainage of Peru was evaluated. The samples were collected from Yanacocha mining (3000 m.a.s.l.) located in the North of Cajamarca region, Yanamina mining (4440 m.a.s.l.) located in the middle of Huancavelica region; finally, SPCC mining (2000 m.a.s.l.) located in the South of Moquegua region. We isolated 11 strains from which three were identified asAcidithiobacillus ferrooxidans, two asAt. ferrivorans, two asAt. ferridurans,three asLeptospirillum ferrooxidansand one asAcidiphilium sp.by comparative sequencing of PCR-amplified 16S rRNA genes. Phylogenetic analysis of the 16S rRNA genes revealed that some of the strains isolated are closely related to other already known, but there are some with similarities lower than < 95 percent. Our results provide the first study on the diversity of iron-oxidizing bacteria isolated from acid mine drainage of Peru.

scholarly journals Development and Application of Small-Subunit rRNA Probes for Assessment of Selected Thiobacillus Species and Members of the Genus Acidiphilium

2000 ◽  
Vol 66 (7) ◽  
pp. 3065-3072 ◽  
Author(s):  
Jordan Peccia ◽  
Eric A. Marchand ◽  
Joann Silverstein ◽  
Mark Hernandez
Keyword(s):  
Acid Mine Drainage ◽  
Mine Drainage ◽  
Small Subunit ◽  
Rrna Genes ◽  
Ribosomal Database Project ◽  
Oligonucleotide Probes ◽  
Small Subunit Rrna ◽  
Variable Regions ◽  
Acid Mine ◽  
Acidophilic Bacteria

ABSTRACT Culture-dependent studies have implicated sulfur-oxidizing bacteria as the causative agents of acid mine drainage and concrete corrosion in sewers. Thiobacillus species are considered the major representatives of the acid-producing bacteria in these environments. Small-subunit rRNA genes from all of the Thiobacillus andAcidiphilium species catalogued by the Ribosomal Database Project were identified and used to design oligonucleotide DNA probes. Two oligonucleotide probes were synthesized to complement variable regions of 16S rRNA in the following acidophilic bacteria:Thiobacillus ferrooxidans and T. thiooxidans(probe Thio820) and members of the genus Acidiphilium(probe Acdp821). Using 32P radiolabels, probe specificity was characterized by hybridization dissociation temperature (Td ) with membrane-immobilized RNA extracted from a suite of 21 strains representing three groups of bacteria. Fluorochrome-conjugated probes were evaluated for use with fluorescent in situ hybridization (FISH) at the experimentally determinedTd s. FISH was used to identify and enumerate bacteria in laboratory reactors and environmental samples. Probing of laboratory reactors inoculated with a mixed culture of acidophilic bacteria validated the ability of the oligonucleotide probes to track specific cell numbers with time. Additionally, probing of sediments from an active acid mine drainage site in Colorado demonstrated the ability to identify numbers of active bacteria in natural environments that contain high concentrations of metals, associated precipitates, and other mineral debris.

scholarly journals Selective Removal of As(V) Ions from Acid Mine Drainage Using Polymer Inclusion Membranes

Minerals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 909
Author(s):  
Iwona Zawierucha ◽  
Anna Nowik-Zajac ◽  
Grzegorz Malina
Keyword(s):  
Acid Mine Drainage ◽  
Toxic Metals ◽  
Arsenic Removal ◽  
Mine Drainage ◽  
Environmental Pollutants ◽  
Sulfuric Acid Concentration ◽  
Toxic Metal ◽  
Cellulose Triacetate ◽  
Selective Removal ◽  
Acid Mine

Acid mine drainage (AMD) is globally recognized as one of the environmental pollutants of the priority concern due to high concentrations of toxic metals and sulfates. More rigorous environmental legislation requires exploitation of effective technologies to remove toxic metals from contaminated streams. In view of high selectivity, effectiveness, durability, and low energy demands, the separation of toxic metal ions using immobilized membranes with admixed extractants could ameliorate water quality. Cellulose triacetate based polymer inclusion membranes (PIMs), with extractant and plasticizer, were studied for their ability to transport of As(V) ions from synthetic aqueous leachates. The effects of the type and concentration of extractant, plasticizer content, and sulfuric acid concentration in source phase on the arsenic removal efficiency have been assessed. Under the best of applied conditions, PIM with Cyanex 921 as extractant and o-nitrophenyl octyl ether (o-NPOE) as plasticizer showed high repeatability and excellent transport activity for selective removal of As(V) from AMD.

Role of microalgae in treatment of acid mine drainage and recovery of valuable metals

2020 ◽  
Vol 30 ◽  
pp. 346-350 ◽  
Author(s):  
D.P. Krishna Samal ◽  
Lala Behari Sukla ◽  
Archana Pattanaik ◽  
Debabrata Pradhan
Keyword(s):  
Acid Mine Drainage ◽  
Mine Drainage ◽  
Acid Mine ◽  
Valuable Metals

scholarly journals Acid neutralizing capacity minerals in Barani Pit PT Agincourt Resources Martabe, North Sumatera: alternative agent on neutralizing acid mine drainage

2020 ◽  
Vol 202 ◽  
pp. 02008
Author(s):  
Rinal Khaidar Ali ◽  
Lestari Butar-butar ◽  
Nurakhmi Qadaryati ◽  
Narulita Santi
Keyword(s):  
Acid Mine Drainage ◽  
Mine Drainage ◽  
Environmental Pollutants ◽  
Acid Neutralizing Capacity ◽  
Geological Condition ◽  
Bearing Material ◽  
Acid Mine ◽  
Rock Formations ◽  
Neutralizing Capacity ◽  
Alternative Agent

Acid Mine Drainage (AMD) is produced when sulfide-bearing material is exposed to oxygen and water. The mine may accelerate this natural reaction due to the wide and rapid exposure of sulfide minerals. Although economical ores are considered environmental pollutants, there are several elements that act as acid neutralizing capacity (ANC) mineral are associated with the ore, especially silicate minerals. Currently, PT Agincourt Resources Martabe has only relying on the use of carbonates mineral groups to handle the AMD issue. In fact, the geological condition in this area is formed of typically alumina-rich and alkaline minerals which are capable to neutralize the AMD as well. The aim of this study is to determine ANC minerals from the rock formations that has impact to neutralize the AMD in Barani Pit. The methods are geological field observation, mineral determination using spectral analysis, and assay analysis that provides data to sort the recommended ANC minerals. The ANC minerals from the rock formations and wastes within Barani Pit, other than calcite, are muscovite, illite, smectite, kaolinite, dickite and gypsum. The knowledge about these alternative ANC minerals might give contribution to reduce and solve the risk of acid mine drainage polluting the environment.

Recovery of valuable metals from acid mine drainage by selective titration

1983 ◽  
Vol 17 (11) ◽  
pp. 1585-1590 ◽  
Author(s):  
Dennis R. Jenke ◽  
Frank E. Diebold
Keyword(s):  
Acid Mine Drainage ◽  
Mine Drainage ◽  
Acid Mine ◽  
Valuable Metals
Sign in / Sign up

Export Citation Format

Share Document