scholarly journals Genetic variation responsible for mouse strain differences in integrin α2 expression is associated with altered platelet responses to collagen

Blood ◽  
2004 ◽  
Vol 103 (9) ◽  
pp. 3396-3402 ◽  
Author(s):  
Tong-Tong Li ◽  
Susana Larrucea ◽  
Shiloe Souza ◽  
Suzanne M. Leal ◽  
José A. López ◽  
...  
Keyword(s):  
Human Platelet ◽  
Strain Differences ◽  
Genetic Alterations ◽  
Mouse Strains ◽  
Lag Phase ◽  
Lod Score ◽  
Log Odds ◽  
Integrin Α2 ◽  
Mouse System ◽  
Mouse Strain Differences

Abstract As mouse models have become commonplace for studying hemostasis and thrombosis, we considered whether the mouse system had utility for assessing genetic alterations in platelet receptors. Platelets from 5 mouse strains (C57BL/6 [C57], FVB/N [FVB], BALB/c, C3H/He, and 129Sv) showed only minor differences in the expression of integrin αIIb, integrin β3, glycoprotein (GP) Ibα, or GPVI across strains. However, FVB platelets expressed approximately 50% the level of integrin α2 as platelets from other strains (P < .0001). We bred FVB mice with C57 and assessed α2 expression in FVB/C57xFVB/C57 (F2) offspring. Linkage analysis demonstrated the gene responsible for α2 levels is tightly linked to the D13mit260 marker (log odds [lod] score 6.7) near the α2 gene. FVB platelets showed reduced aggregation and a longer lag phase to collagen. FVB and C57 platelets aggregated similarly to collagen-related peptide, but FVB platelets showed a reduction in rhodocytin-induced Syk and PLCγ2 tyrosine phosphorylation. Thus, FVB platelets express half the level of α2 as other mouse strains, a trait linked to the α2 gene and seemingly responsible for reduced platelet aggregation to collagen. These strain differences serve as a useful model for the 2-fold difference in human platelet α2β1 expression and demonstrate that α2β1 participates in signaling during platelet activation. (Blood. 2004;103:3396-3402)

Mouse strain differences in resident peritoneal cells: a flow cytometric analysis

1990 ◽  
Vol 24 (1) ◽  
pp. 53-62 ◽  
Author(s):  
Michael F. W. Festing ◽  
R. Legg ◽  
T. Eydmann ◽  
A. Brammall
Keyword(s):  
Mouse Strain ◽  
Total Yield ◽  
Flow Cytometric Analysis ◽  
Strain Differences ◽  
Mouse Strains ◽  
Peritoneal Cells ◽  
Flow Cytometric ◽  
Fluorescent Beads ◽  
Flow Cytometric Study ◽  
Mouse Strain Differences

A flow-cytometric study of resident peritoneal cells among 8 mouse strains showed a more than twofold variation in the ratio of macrophages to macrophages plus lymphocytes, ranging from 27% in A/J to 62% in C57B/L10, with significant strain differences in a number of other cellular parameters. There was a particular deficiency of lymphocytes in strain CBA/N, which carries the xid mutation. Studies of the phagocytosis of fluorescent beads also revealed large differences in the number of beads taken up, ranging from 0·99 per cell in MFI to 1·64 per cell in BALB/c mice in a 20-min period. The total number of peritoneal cells collected also varied between strains, ranging from 2·75 × 106 in CBA/Ca to 5·85 × 106 in MFI. The total yield of macrophages per mouse ranged from 0·93 × 106 in A/J to 3·16 × 106 in C57BL/10. These differences should be taken into account when designing experiments which use resident peritoneal cells.

scholarly journals Mouse strain differences in autonomic responses to stress

2006 ◽  
Vol 5 (2) ◽  
pp. 139-149 ◽  
Author(s):  
M. J. V. Van Bogaert ◽  
L. Groenink ◽  
R. S. Oosting ◽  
K. G. C. Westphal ◽  
J. van der Gugten ◽  
...  
Keyword(s):  
Mouse Strain ◽  
Strain Differences ◽  
Autonomic Responses ◽  
Mouse Strain Differences

Ethanol-induced mouse strain differences in locomotor activity

1975 ◽  
Vol 3 (3) ◽  
pp. 533-535 ◽  
Author(s):  
Carrie L. Randall ◽  
John A. Carpenter ◽  
David Lester ◽  
Howard J. Friedman
Keyword(s):  
Locomotor Activity ◽  
Mouse Strain ◽  
Strain Differences ◽  
Mouse Strain Differences

scholarly journals Mouse Strain Differences in Plasmacytoid Dendritic Cell Frequency and Function Revealed by a Novel Monoclonal Antibody

2003 ◽  
Vol 171 (12) ◽  
pp. 6466-6477 ◽  
Author(s):  
Carine Asselin-Paturel ◽  
Géraldine Brizard ◽  
Jean-Jacques Pin ◽  
Francine Brière ◽  
Giorgio Trinchieri
Keyword(s):  
Monoclonal Antibody ◽  
Dendritic Cell ◽  
Mouse Strain ◽  
Plasmacytoid Dendritic Cell ◽  
Strain Differences ◽  
Cell Frequency ◽  
And Function ◽  
Mouse Strain Differences

Mouse Strain Differences in in vivo and in vitro Immunosuppressive Effects of Opioids

1995 ◽  
pp. 115-121 ◽  
Author(s):  
Toby K. Eisenstein ◽  
Joseph J. Meissler ◽  
Jeanine L. Bussiere ◽  
Thomas J. Rogers ◽  
Ellen B. Geller ◽  
...  
Keyword(s):  
Mouse Strain ◽  
Strain Differences ◽  
Mouse Strain Differences

Mouse strain differences in operant self-administration of ethanol

1987 ◽  
Vol 17 (5) ◽  
pp. 439-451 ◽  
Author(s):  
Gregory I. Elmer ◽  
Richard A. Meisch ◽  
Frank R. George
Keyword(s):  
Mouse Strain ◽  
Strain Differences ◽  
Self Administration ◽  
Mouse Strain Differences

Mechanism for mouse strain differences in the protective effect of Sudan III against the in vivo genotoxicity of 7,12-dimethylbenz[a]anthracene

1996 ◽  
Vol 89 (3) ◽  
pp. 231-239 ◽  
Author(s):  
Shigeki Hatakeyama ◽  
Yayoi Hayasaki ◽  
Makihiko Masuda ◽  
Akio Kazusaka ◽  
Shoichi Fujita
Keyword(s):  
Protective Effect ◽  
Mouse Strain ◽  
Strain Differences ◽  
Sudan Iii ◽  
Mouse Strain Differences

Endotoxin-Induced Platelet Activation in Human Whole Blood In Vitro

1988 ◽  
Vol 59 (03) ◽  
pp. 378-382 ◽  
Author(s):  
Gyorgy Csako ◽  
Eva A Suba ◽  
Ronald J Elin
Keyword(s):  
Platelet Aggregation ◽  
Whole Blood ◽  
Human Platelet ◽  
Platelet Rich Plasma ◽  
Atp Release ◽  
Human Platelets ◽  
Lag Phase ◽  
Human Whole Blood ◽  
Dose Dependent

SummaryThe effect of purified bacterial endotoxin was studied on human platelets in vitro. In adding up to 1 μg/mL of a highly purified endotoxin, we found neither aggregation nor ATP release in heparinized or citrated human platelet-rich plasma. On the other hand, endotoxin at concentrations as low as a few ng/mL (as may be found in septic patients) caused platelet aggregation in both heparinized and citrated human whole blood, as monitored by change in impedance, free platelet count, and size. Unlike collagen, the platelet aggregation with endotoxin occurred after a long lag phase, developed slowly, and was rarely coupled with measurable release of ATP. The platelet aggregating effect of endotoxin was dose-dependent and modified by exposure of the endotoxin to ionizing radiation. Thus, the activation of human platelets by “solubilized” endotoxin in plasma requires the presence of other blood cells. We propose that the platelet effect is mediated by monocytes and/or neutrophils stimulated by endotoxin.

scholarly journals Age- and Strain-Related Differences in Bone Microstructure and Body Composition During Development in Inbred Male Mouse Strains

2020 ◽  
Vol 106 (4) ◽  
pp. 431-443
Author(s):  
Maria Papageorgiou ◽  
Ursula Föger-Samwald ◽  
Katharina Wahl ◽  
Katharina Kerschan-Schindl ◽  
Peter Pietschmann
Keyword(s):  
Body Composition ◽  
Skeletal Development ◽  
Strain Differences ◽  
Bone Microstructure ◽  
Mouse Strains ◽  
Strain Specificity ◽  
Development Patterns ◽  
Fat Depots ◽  
The Masses ◽  
Early Strain

AbstractWe explored age- and strain-related differences in bone microstructure and body composition in male C57BL/6J, DBA/2JRj and C3H/J mice. Bone microstructure of the femur, tibia and L4 was assessed by μCT at the age of 8, 16 and 24 weeks. The weight of several muscles and fat depots were measured at the same time points. At all timepoints, C3H/J mice had the thickest cortices followed by DBA/2JRj and C57BL/6J mice. Nevertheless, C57BL/6J mice had higher Tb.BV/TV and Tb.N, and lower Tb.Sp than DBA/2JRj and C3H/J mice at least at 24 weeks of age. Skeletal development patterns differed among strains. C57BL/6J and DBA/2JRj mice, but not C3H/J mice, experienced significant increases in the sum of the masses of 6 individual muscles by 24 weeks of age. In C57BL/6J and DBA/2JRj mice, the mass of selected fat depots reached highest values at 24 weeks, whist, in C3H/J mice, the highest values of fat depots masses were achieved at 16 weeks. Early strain differences in muscle and fat masses were largely diminished by 24 weeks of age. C3H/J and C57BL/6J mice displayed the most favorable cortical and trabecular bone parameters, respectively. Strain differences in body composition were less overt than strain specificity in bone microstructure, however, they possibly influenced aspects of skeletal development.

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