scholarly journals Graft-vs.-Host Activity of Thymocytes: Relationship to the Role of Thymocytes in Hemopoiesis

Blood ◽  
1972 ◽  
Vol 39 (6) ◽  
pp. 850-861 ◽  
Author(s):  
Joan Wright Goodman ◽  
Kay T. Burch ◽  
Nancy L. Basford
Keyword(s):  
Lymph Node ◽  
F1 Hybrid ◽  
Radiation Chimeras ◽  
Lymph Node Cells ◽  
Per Se ◽  
Uptake Method ◽  
Better Than

Abstract The importance of graft-vs.-host (GVH) activity to the ability of thymocytes to augment hemopoiesis in radiation chimeras was investigated. Parental (P) lymph node cells were found by the 59Fe-uptake method not to have an analogous augmentative effect. When thymus donor, marrow donor, and irradiated recipient were chosen immunogenetically so that GVH could occur in either the presence or absence of graft-vs.-graft (GVG) activity, it was seen that GVH reactivity per se resulted in no improvement of marrow growth. However, when P thymocytes specifically tolerant to an F1 hybrid host were administered with P marrow, augmentation was three times greater than when nontolerant P thymocytes were given. It was concluded that GVH activity not only is not essential but actually is detrimental to augmentation. Ninety-day survival of chimeras given specifically tolerant P thymocytes was better than that of mice given P marrow only and very much better than those given marrow and nontolerant thymocytes.

scholarly journals Clonal analysis of F1 hybrid helper T cells restricted to parental or F1 hybrid major histocompatibility determinants.

1980 ◽  
Vol 152 (4) ◽  
pp. 920-930 ◽  
Author(s):  
J F Sproviero ◽  
M J Imperiale ◽  
M Zauderer
Keyword(s):  
T Cells ◽  
Lymph Node ◽  
Positive Culture ◽  
Keyhole Limpet Hemocyanin ◽  
Helper T Cells ◽  
Major Histocompatibility ◽  
F1 Hybrid ◽  
Helper Activity ◽  
Lymph Node Cells ◽  
Limiting Dilution

Antigen-specific major histocompatibility complex (MHC)-restricted helper T cell precursors were induced to proliferate in cultures of keyhole limpet hemocyanin-primed lymph node cells. Clones of F1 hybrid helper T cells were isolated in limiting-dilution cultures. Each positive culture at a limiting-dilution of lymph node cells gave rise to > 10 helper T cells with a single MHC-restricted specificity. This made it possible to independently assay specific helper activity of isolated clones in secondary cultures with B cells of diverse origin. Different clones with helper activity restricted to either parental or unique F1 hybrid MHC determinants were found to occur at approximately equal frequency. The results are discussed in relation to hybrid Ia specificities and dual-complementing MHC-linked Ir genes.

scholarly journals CELLULAR REQUIREMENTS FOR THE REJECTION OF SKIN ALLOGRAFTS IN RATS

1973 ◽  
Vol 138 (2) ◽  
pp. 331-341 ◽  
Author(s):  
David M. Lubaroff
Keyword(s):  
Bone Marrow ◽  
Lymph Node ◽  
Bone Marrow Cells ◽  
Lymph Node Cell ◽  
Skin Reactions ◽  
Skin Allografts ◽  
Lymph Node Cells ◽  
Bone Marrow Derived Cells ◽  
Transplantation Antigens

The role of bone marrow-derived cells in the rejection of skin allografts in rats was investigated. Lewis rats, rendered tolerant of BN antigens and bearing healthy grafts, were thymectomized, irradiated with 900 rad, and injected with varying doses of either normal isologous bone marrow, normal lymph node cells, and/or lymph node cells presensitized to BN antigens. In some experiments rats were also adoptively sensitized to tuberculin. Results showed that, although necessary for the elicitation of tuberculin skin reactions, bone marrow cells are not needed for the rejection of previously tolerated skin allografts. Rats receiving lymph node cells alone rejected their grafts in about 6–7 days. In addition, rats injected with bone marrow alone also rejected their grafts, although significantly later than did lymph node cell recipients, indicating that rat marrow contains a population of cells capable of reacting to transplantation antigens. These cells were found capable of reacting to major transplantation antigens but not minor as they were ineffective in causing the rejection of Ag-B compatible Fischer skin grafts. From experiments utilizing bone marrow from neonatally thymectomized donors and cells treated with an antiserum to rat T cells, these competent cells in the marrow were shown to be thymus derived.

scholarly journals Evidence for a pathogenic role of a cell-mediated immune mechanism in experimental glomerulonephritis.

1978 ◽  
Vol 148 (1) ◽  
pp. 246-260 ◽  
Author(s):  
A K Bhan ◽  
E E Schneeberger ◽  
A B Collins ◽  
R T McCluskey
Keyword(s):  
Endothelial Cells ◽  
Lymph Node ◽  
Gamma Globulin ◽  
Mononuclear Cells ◽  
Immune Mechanism ◽  
Pathogenic Role ◽  
Glomerular Lesions ◽  
A Cell ◽  
Lymph Node Cells

Lewis rats were injected intravenously with rabbit anti-rat glomerular basement membrane (GBM) antisera in doses that were sufficient to cause glomerular fixation of rabbit gamma globulin (RGG) detectable by immunofluorescence, but which failed to induce histologically detectable lesions. 24 h later, groups of rats received lymph node cells or serum from syngeneic donors that had been immunized with either RGG or ovalbumin; they were injected with [3H]thymidine three times during the next 2 days, and sacrificed 48 or 96 h after transfer. Only the rats given anti-GBM antiserum plus lymph node cells from donors sensitized to RGG showed histological glomerular lesions, in the form of segmental hypercellularly and necrosis. Autoradiographs revealed the greatest number of labeled cells in glomeruli in the same group. In analogous experiments, it was shown that T-cell-enriched populations could induce hypercellular glomerular reactions. On the basis of electronmicroscopic and autoradiographic observations, it appears that the glomerular hypercellularity resulted from both infiltration of mononuclear cells and proliferation of endothelial cells. The findings indicate that interaction of specifically sensitized lymphocytes with glomerular-bound antigen can induce a cell-mediated (delayed-type) reaction in glomeruli.

scholarly journals THE LYMPHATIC STATUS OF HAMSTER CHEEK POUCH TISSUE IN RELATION TO ITS PROPERTIES AS A GRAFT AND AS A GRAFT SITE

1971 ◽  
Vol 133 (3) ◽  
pp. 620-639 ◽  
Author(s):  
Clyde F. Barker ◽  
R. E. Billingham
Keyword(s):  
Lymph Node ◽  
Normal Skin ◽  
Cheek Pouch ◽  
Donor Strain ◽  
Skin Grafts ◽  
Hamster Cheek Pouch ◽  
Regional Lymph Nodes ◽  
F1 Hybrid ◽  
Lymph Node Cells ◽  
Trunk Skin

Hamster cheek pouch skin, transplanted to the side of an isogenic host's chest wall, retains its immunologically privileged status as evidenced by the prolonged survival of inlaid homografts of ordinary skin. Various findings sustain the premise that exemption from rejection by otherwise susceptible homografts in both intact pouch tissue and in established pouch skin isografts is due to an impediment in the afferent pathway of the immunologic reflex, i.e., to deficient lymphatic drainage. Although lymphatics were not apparent when dye was injected into pouch skin grafts or into grafts of ordinary skin sustained by them, lymph vessels were readily and consistently revealed by dye injected into intact trunk skin or established isografts of trunk skin. When suspensions of viable lymph node cells from specifically sensitized parental strain donors were injected superficially into either the intact skin or established grafts of normal skin on F1 hybrid hamsters, a striking hypertrophy of the regional lymph nodes occurred, due to graft-versus-host reactivity. However, similar cell suspensions inoculated into intact pouch tissue or into pouch skin grafts on F1 hamsters incited no regional lymphadenopathy, indicating the lack of appropriate pathways to the nodes. When skin homografts were inlaid eccentrically into pouch skin isografts, so that they were in contact with host skin at one edge, rejection occurred. Furthermore, rejection of long-established intrapouch skin homografts resulted if the hosts received: (a) small homografts of ordinary skin transplanted to conventional beds; (b) suspensions of donor strain pouch skin epidermal cells, injected intracutaneously; (c) lymph node cells from specifically sensitized donors of the same strain, i.e. adoptive immunization; or, (d) if a portion of the target homograft's perimeter was surgically approximated to body skin. Treatment of normal hamsters with two closely spaced pulses of ALS, although only marginally effective in prolonging the lives of homografts of trunk skin, enabled pouch skin homografts to survive for very long periods. The influence of this brief treatment with immunosuppressant was still demonstrable if challenge of hosts with the weakly immunogenic pouch skin homografts was delayed for 100 days.

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