Development of antithrombin antibodies following surgery in patients with prosthetic cardiac valves [see comments]

Although antibody inhibitors directed against blood coagulation factors are well known, antibody inhibitors directed against thrombin are rare. We describe three postsurgical patients with prosthetic cardiac valves who developed serum autoantibodies reactive with human and bovine thrombin, as demonstrated by coagulation studies and immunoblotting. Despite marked prolongation of the thrombin time in these patients, the inhibitors were not associated with significant clinical bleeding. The mechanism of antithrombin autoantibody formation following surgery in patients with prosthetic cardiac valves remains to be determined.

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Development of antithrombin antibodies following surgery in patients with prosthetic cardiac valves [see comments]

Blood ◽

10.1182/blood.v72.4.1375.1375 ◽

1988 ◽

Vol 72 (4) ◽

pp. 1375-1380 ◽

Cited By ~ 55

Author(s):

RB Stricker ◽

PK Lane ◽

JD Leffert ◽

GM Rodgers ◽

MA Shuman ◽

...

Keyword(s):

Blood Coagulation ◽

Coagulation Factors ◽

Thrombin Time ◽

Cardiac Valves ◽

Blood Coagulation Factors ◽

Bovine Thrombin ◽

Autoantibody Formation

Abstract Although antibody inhibitors directed against blood coagulation factors are well known, antibody inhibitors directed against thrombin are rare. We describe three postsurgical patients with prosthetic cardiac valves who developed serum autoantibodies reactive with human and bovine thrombin, as demonstrated by coagulation studies and immunoblotting. Despite marked prolongation of the thrombin time in these patients, the inhibitors were not associated with significant clinical bleeding. The mechanism of antithrombin autoantibody formation following surgery in patients with prosthetic cardiac valves remains to be determined.

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Influence Of Elastase On Blood Coagulation Factors: Studies In Vitro, In Rats And In Göttinger Miniatur Pigs

10.1055/s-0038-1653045 ◽

1981 ◽

Author(s):

U Kasten ◽

U Artmann ◽

T Kaethner ◽

H Burchardi ◽

H Köstering

Keyword(s):

Blood Coagulation ◽

Coagulation Factors ◽

Bleeding Complications ◽

Thrombin Time ◽

Normal Range ◽

Blood Coagulation Factors ◽

Acute Respiratory Insufficiency ◽

Coagulation Tests ◽

Antifibrinolytic Drugs

The influence of blood coagulation factors in pat. with acute respiratory insufficiency of adults, especially of the so called “pancreatitis lungs” is still unknown. In order to find out the effect of elastase, possibly activated by trypsin in pat. with acute pancreatitis, on blood coagulation factors, we performed some studies. In vitro elastase induces in plasma and blood in correlation to the dosages Enhancement of thrombingeneration in the TGT, a shortening of PTT, Thrombin time and of r- and k-time in the TEG, a loss of fibrinogen and an increase of fibrinmono-mercomplexes. In another study, elastase (960 U/ kg b.w.) was injected intravenously in rats. 30 min. later there was found a loss of fibrinogen, number of platelets, Prothrombin and a prolongation of PTT and Thrombin time and an increase of fibrinomonomercomplexes, especially in these rats, which received beside elastase Kalikreininhibitors or antifibrinolytic drugs. After repeated injections (3 times within 30 h) we found histomorpholgically thrombi as well as bleeding complications. In another study we performed (150 min) an infusion of elastase (333 U/kg b.w./h) to 9 pigs. We determined a loss of fibrinogen of platelets, of F. II, F. VII and F. XIII, a prolongation of PTT. F. VIII and F. V remained within the normal range But there was found an enhancement of Thrombin generation in the TGT, too. Compariening the results of blood coagulation tests and of histomorphological findings, elastase induced a DIC. We have to discuss their influence on ARIA and “Pancreatic lungs”.

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Alterations of Blood Coagulation in Patients with Pancreatic Disorders

10.1055/s-0039-1682354 ◽

1977 ◽

Author(s):

H. Kästering ◽

M. Hasenbein ◽

C. Wendt ◽

P.G. Lankisch

Keyword(s):

Blood Coagulation ◽

Coagulation Factors ◽

Pancreatic Cysts ◽

Thrombin Time ◽

Consumption Coagulopathy ◽

Blood Coagulation Factors ◽

The Past ◽

Α2 Macroglobulin ◽

Pancreatic Disorders ◽

Chronic Relapsing Pancreatitis

Hemorrhage as uell as thromboembolism is often found in patients uith pancreatic diseases. Ide systematically examined in the past tuo years blood coagulation factors in Ikpatients, uho uere admitted to our clinic uith elevated levels of amylases or uho were transfered from other hospitals after complications had occured.5B patients uith increased amylases (20 patients uith acute pancreatitis, 25 uith chronic relapsing pancreatitis, 2 uith concomitant pancreatitis, 11 uith pancreatic cysts) shoued no severe complications. In comparison to 26 normal persons ue found significant differences in the follouing results: enhancement of the thrombin generation in the TGT, shortening of the PTT and of the r- and k-time in the TEG, louered levels of α1-antitrypsin, α2-macroglobulin and heparin-like substances (Hiepler Test), increased levels in fibrinogen and fibrin monomere complexes, and decrease in platelets and in Quick’s Test. There uere no changes in the thrombin time, antithrombin III and plasminogen. In the group of 16 patients uith complications there uere only 3 cases of hypercoagulability, all others shoued signs of consumption-coagulopathy. The nine patients uho died all shoued signs Df acute renal failure and pancreatic lungs. Ue believe that the disturbances of blood coagulation in patients uith increased levels of amylases are initially caused by hypercoagulability and lead in some cases to thromboembolism, consumption-coagulopathy and DIC of the lungs and kidneys.

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Changes in the blood coagulation system that determine postoperative complications in patients with non-tumor mechanical jaundice

Grekov s Bulletin of Surgery ◽

10.24884/0042-4625-2021-180-2-12-20 ◽

2021 ◽

Vol 180 (2) ◽

pp. 12-20

Author(s):

V. E. Fedorov ◽

B. S. Kharitonov ◽

A. D. Aslanov ◽

O. E. Logvina ◽

M. S. Narizhnaya

Keyword(s):

Postoperative Complications ◽

Blood Coagulation ◽

Blood Platelets ◽

Coagulation Factors ◽

Coagulation System ◽

Thrombin Time ◽

Blood Coagulation Factors ◽

Blood Coagulation System ◽

Normal Limits ◽

Prothrombin Index

The OBJECTIVE was to study the features of changes in the blood coagulation system that contribute to the development of postoperative complications in patients depending on the stage of non-tumor mechanical jaundice at admission.METHODS AND MATERIALS. A total of 537 patients with mechanical jaundice were examined and changes in the blood coagulation system were analyzed. Vascular-platelet hemostasis was characterized by the following tests: capillary resistance, the number of desquamated endothelial cells, the number of blood platelets. Plasma hemostasis was analyzed using activated partial thromboplastin time, plasma soluble fibrin level, thrombin time, prothrombin ratio, prothrombin index, and fibrinogen blood level. Then, XIIa-dependent fibrinolysis in the blood and the level of the fibrin D-dimer in the blood plasma were determined.RESULTS. It was found that in the first stage of mechanical jaundice, with cholestasis, there were no changes in blood coagulation system that go beyond the normal limits. In the second stage, during cytolysis of hepatocytes, hyperbilirubinemia and hypertransaminasemia contribute to the activation of platelet first, and then plasma hemostasis. In the third stage (cholangitis), the death of endotheliocytes increases and there is a deficiency of blood coagulation factors due to their consumption and increased fibrinolysis.CONCLUSION. In the stage of cholestasis in patients with non-tumors mechanical jaundice, the parameters of the coagulation system remain within the reference values. In the stage of cytolysis, as endotheliotoxicosis increases, platelet and plasma hemostasis begins to activate, which can lead to thrombosis and thromboembolism in vital organs. In the stage of cholangitis, further activation of plasma hemostasis causes hemorrhagic syndrome. The occurrence of the described disorders in blood coagulation system with the progression of MJ dictates the need to monitor the changes in the blood coagulation system and their correction for the prevention of intra-and postoperative complications.

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Natural Oestrogen in the Female Climacteric - Influence on Blood Coagulation and Fibrinolysis

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648686 ◽

1978 ◽

Vol 40 (02) ◽

pp. 532-541 ◽

Cited By ~ 2

Author(s):

Anders Lagrelius ◽

Nils-Olov Lunell ◽

Margareta Blombäck

Keyword(s):

Blood Coagulation ◽

Antithrombin Iii ◽

Coagulation Factors ◽

Control Group ◽

Blood Coagulation Factors ◽

Excessive Bleeding ◽

Oestrone Sulphate ◽

Menopausal Women ◽

History Of ◽

Coagulation And Fibrinolysis

SummaryThe aim of the present study was to investigate the effect on blood coagulation and fibrinolysis of a natural oestrogen preparation, piperazine oestrone sulphate, prospectively in menopausal women. Scopolamine was given to the control group.The women were investigated before and during treatment with regard to factors VIII, VII, X, V, fibrinopeptide A, antithrombin III, plasminogen, rapid antiplasmin and α1-antitrypsin. There was no significant change towards hypercoagulability or decreased fibrinolysis in any group. In the oestrogen group, however, a tendency towards an increased level of plasminogen and a decreased level of antiplasmin was demonstrated. In the scopolamine group there was an unexpected fall in factors X and V and also in plasminogen and α1,-antitrypsin. A low level of some blood coagulation factors in some of the women before treatment is somewhat astonishing; none of them had any history of excessive bleeding.

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STEROIDSTRUKTUR UND LEBERTOXIZITÄT

Acta Endocrinologica ◽

10.1530/acta.0.0540073 ◽

1967 ◽

Vol 54 (1) ◽

pp. 73-84 ◽

Cited By ~ 7

Author(s):

H. L. Krüskemper ◽

G. Noell

Keyword(s):

Alkaline Phosphatase ◽

Liver Function ◽

Blood Coagulation ◽

Electrophoretic Pattern ◽

Coagulation Factors ◽

Blood Coagulation Factors ◽

Methyl Group ◽

Liver Functions ◽

Male Subjects ◽

Androstane Derivatives

ABSTRACT In male subjects investigations have been carried out regarding the effect of C1- and C17-methylated androstane derivatives (20 mg per day, orally, two weeks) on liver functions (parameters: activities of GPT, GOT, alkaline phosphatase and cholinesterase in serum; electrophoretic pattern; blood coagulation factors V, VII, X and prothrombin; BSP-retention). In addition to the well known hepatotropic action of 17α-alkylated C-19-steroids a quasi-axial 1α-methyl configuration (in 1α-methylandrost-2-en-17β-ol) definitely increased BSP-retention and several coagulation factors. These steroid effects decreased gradually when a methyl group was introduced in C1 equatorially (1-methylandrost-1-en-17β-ol-3-one) or quasi-equatorially (1β-methylandrost-2-en-17β-ol), the latter compound completely lacking from any influence on parameters of liver function under investigation.

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Localization of the structural difference between bovine blood coagulation factors X1 and X2 to tyrosine 18 in the activation peptide.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)35614-4 ◽

1986 ◽

Vol 261 (9) ◽

pp. 4008-4014 ◽

Cited By ~ 2

Author(s):

T Morita ◽

C M Jackson

Keyword(s):

Blood Coagulation ◽

Structural Difference ◽

Coagulation Factors ◽

Bovine Blood ◽

Blood Coagulation Factors ◽

Activation Peptide

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EXPRESSION IN ONTOGENESIS OF HUMAN BLOOD COAGULATION FACTORS

10.1055/s-0038-1644610 ◽

1987 ◽

Author(s):

H J Hassan ◽

A Leonardi ◽

C Chelucci ◽

R Guerriero ◽

P M Mannucci ◽

...

Keyword(s):

Blood Coagulation ◽

Protein C ◽

Antithrombin Iii ◽

Liver Homogenate ◽

Coagulation Factor ◽

Coagulation Factors ◽

Factor Ix ◽

Adult Liver ◽

Blood Coagulation Factors ◽

Guanidine Isothiocyanate

We have analyzed the expression of several blood coagulation factors (IX, VIII, X, fibrinogen chains) and inhibitors (antithrombin III, protein C) in human embryonic and fetal livers, obtained from legal abortions at 6-11 week post-conception. The age was established by morphologic staging and particularly crown-rump lenght measurement.Total cellular RNA was isolated from partially purified hepatocytes or total liver homogenate using the guanidine isothiocyanate method. Poly(A)+ RNA was selected by oligodT cellulose chromatography. The size and the number of the embryonic and fetal transcripts are equivalent to those observed in adult liver, as evaluated by Northern blot analysis of total or poly(A)+ RNA hybridized to human cDNA probes.The level of coagulation factor transcripts in embryonic and fetal liver was evaluated by dot hybridization of total RNA (0.5-10 ug), as compared to RNA extracted from normal adult liver biopsies. The expression of blood coagulation factors in embryos is generally reduced for all factors, but at a different degree. In 5-11 wk liver, the level of factor IX is 5-10% of that observed in adults, while fibrinogen, protein C, antithrombin III RNA level rises from 25 to 50% and factor X is expressed at a level comparable to that observed in adult liver.We conclude that during these stages of development blood coagulation factors are expressed according to three different time, curves, possibly due to the effect of different types of regulatory mechanisms.

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