Influence Of Elastase On Blood Coagulation Factors: Studies In Vitro, In Rats And In Göttinger Miniatur Pigs

1981 ◽  
Author(s):  
U Kasten ◽  
U Artmann ◽  
T Kaethner ◽  
H Burchardi ◽  
H Köstering
Keyword(s):  
Blood Coagulation ◽  
Coagulation Factors ◽  
Bleeding Complications ◽  
Thrombin Time ◽  
Normal Range ◽  
Blood Coagulation Factors ◽  
Acute Respiratory Insufficiency ◽  
Coagulation Tests ◽  
Antifibrinolytic Drugs

The influence of blood coagulation factors in pat. with acute respiratory insufficiency of adults, especially of the so called “pancreatitis lungs” is still unknown. In order to find out the effect of elastase, possibly activated by trypsin in pat. with acute pancreatitis, on blood coagulation factors, we performed some studies. In vitro elastase induces in plasma and blood in correlation to the dosages Enhancement of thrombingeneration in the TGT, a shortening of PTT, Thrombin time and of r- and k-time in the TEG, a loss of fibrinogen and an increase of fibrinmono-mercomplexes. In another study, elastase (960 U/ kg b.w.) was injected intravenously in rats. 30 min. later there was found a loss of fibrinogen, number of platelets, Prothrombin and a prolongation of PTT and Thrombin time and an increase of fibrinomonomercomplexes, especially in these rats, which received beside elastase Kalikreininhibitors or antifibrinolytic drugs. After repeated injections (3 times within 30 h) we found histomorpholgically thrombi as well as bleeding complications. In another study we performed (150 min) an infusion of elastase (333 U/kg b.w./h) to 9 pigs. We determined a loss of fibrinogen of platelets, of F. II, F. VII and F. XIII, a prolongation of PTT. F. VIII and F. V remained within the normal range But there was found an enhancement of Thrombin generation in the TGT, too. Compariening the results of blood coagulation tests and of histomorphological findings, elastase induced a DIC. We have to discuss their influence on ARIA and “Pancreatic lungs”.

scholarly journals The effects of fructose diphosphate on routine coagulation tests in vitro

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Tongqing Chen ◽  
Duan Chen ◽  
Lu Chen ◽  
Zhengxu Chen ◽  
Baolong Wang ◽  
...  
Keyword(s):  
Platelet Aggregation ◽  
Blood Coagulation ◽  
Detection System ◽  
Coagulation System ◽  
Thrombin Time ◽  
Aggregation Rate ◽  
Coagulation Testing ◽  
Coagulation Tests ◽  
Routine Coagulation

AbstractTo evaluate the effects of fructose diphosphate (FDP) on routine coagulation tests in vitro, we added FDP into the mixed normal plasma to obtain the final concentration of 0, 1, 2, 3, 4, 5, 6, 10, 15, 20, 25, 30 and 35 mg/mL of drug. Prothrombin time (PT), activated partial thromboplastin time (aPTT), fibrinogen (FBG) and thrombin time (TT) of samples were analyzed with blood coagulation analyzers from four different manufacturers(Sysmex, Stago, SEKISUI and Werfen) and their corresponding reagents, respectively. Before the experiment, we also observed whether there were significant differences in coagulation test results of different lots of reagents produced by each manufacturer. At the same time as the four routine clotting tests, the Sysmex blood coagulation analyzer and its proprietary analysis software were used to detect the change of maximum platelet aggregation rate in platelet-rich plasma after adding FDP (0, 1, 2, 3, 4, 5 and 6 mg/mL). The results of PT, aPTT and TT showed a FDP (0–35 mg/mL) concentration-dependent increase and a FBG concentration-dependent decrease. The degree of change (increase or decrease) varied depending on the assay system, with PT and aPTT being more affected by the Sysmex blood coagulation testing instrument reagent system and less affected by CEKISUI, TT less affected by CEKISUI and more affected by Stago, and FBG less affected by Stago and more affected by Sysmex. The results of PT, aPTT and TT were statistically positively correlated with their FDP concentrations, while FBG was negatively correlated. The correlation coefficients between FDP and the coagulation testing systems of Sysmex, Stago, Werfen and SEKISUI were 0.975, 0.988, 0.967, 0.986 for PT, and 0.993, 0.989, 0.990 and 0.962 for aPTT, 0.994, 0.960, 0.977 and 0.982 for TT, − 0.990, − 0.983, − 0.989 and − 0.954 for FBG, respectively. Different concentrations of FDP (0, 1, 2, 3, 4, 5 and 6 mg/mL) had different effects on the maximum aggregation rate of platelet induced by the agonists of adenosine diphosphate (ADP, 5 µmol/L), arachidonic acid (Ara, 1 mmol/L), collagen (Col, 2.5 µg/mL) and epinephrine (Epi,10 µmol/L), but the overall downward trend was consistent, that is, with the increase of FDP concentration, the platelet aggregation rate decreased significantly. Our experimental study demonstrated a possible effect of FDP on the assays of coagulation and Platelet aggregation, which may arise because the drug interferes with the coagulation and platelet aggregation detection system, or it may affect our in vivo coagulation system and Platelet aggregation function, the real mechanism of which remains to be further verified and studied.

Development of antithrombin antibodies following surgery in patients with prosthetic cardiac valves [see comments]

Blood ◽  
1988 ◽  
Vol 72 (4) ◽  
pp. 1375-1380
Author(s):  
RB Stricker ◽  
PK Lane ◽  
JD Leffert ◽  
GM Rodgers ◽  
MA Shuman ◽  
...  
Keyword(s):  
Blood Coagulation ◽  
Coagulation Factors ◽  
Thrombin Time ◽  
Cardiac Valves ◽  
Blood Coagulation Factors ◽  
Bovine Thrombin ◽  
Autoantibody Formation

Although antibody inhibitors directed against blood coagulation factors are well known, antibody inhibitors directed against thrombin are rare. We describe three postsurgical patients with prosthetic cardiac valves who developed serum autoantibodies reactive with human and bovine thrombin, as demonstrated by coagulation studies and immunoblotting. Despite marked prolongation of the thrombin time in these patients, the inhibitors were not associated with significant clinical bleeding. The mechanism of antithrombin autoantibody formation following surgery in patients with prosthetic cardiac valves remains to be determined.

Influence of Anticoagulant? Antiaggregant and Antifibrinolytic Drugs on the Walker-256-Carcinosarcoma of Rats

1979 ◽  
Author(s):  
H. Köpetering ◽  
R. Hampe ◽  
B. Ludwig ◽  
J. Ganaainski ◽  
H. Ruakowaki
Keyword(s):  
Blood Coagulation ◽  
Coagulation Factors ◽  
Blood Coagulation Factors ◽  
Walker 256 Carcinosarcoma ◽  
Anticoagulant Drugs ◽  
Walker 256 Tumour ◽  
Antifibrinolytic Drugs ◽  
Walker 256 ◽  
Different Doses

In order to find out, if there ia en influence of blood coagulation factors on tumura we determinad the effects of various anticoagulant drugs (Phenprocoumon, Arvinr , Acet.-salicyl.-acid) and the antifibrinolytics (EACA) on the development, metastasia formation and the ratxa of survival of the walker-256-tumour. Tumorcells were the subcutanously injected upon the back of the tail of female Whistar rats in a total number of 850. We tested the effect of Marcoumar by weekly controls of Quick’s test of every single rat. 52 % of the control survived, while up to 96 % of the rats which were treated with different doses of Marcoumar survived. (n=23 in each group). In another series only 25 % of control-anmals sunaved, but 64 % of the rats, which were treated with EACA and with ASA, and 33 % of the rats which were treated with Arvin, survived (n= 2-7 ,in every group) The spread of the tumour in Arvin-treated rats was most invasible. The effects of clotting mechanism on the four stages in the spreding tumour are being discussed.

scholarly journals Development of antithrombin antibodies following surgery in patients with prosthetic cardiac valves [see comments]

Blood ◽  
1988 ◽  
Vol 72 (4) ◽  
pp. 1375-1380 ◽  
Author(s):  
RB Stricker ◽  
PK Lane ◽  
JD Leffert ◽  
GM Rodgers ◽  
MA Shuman ◽  
...  
Keyword(s):  
Blood Coagulation ◽  
Coagulation Factors ◽  
Thrombin Time ◽  
Cardiac Valves ◽  
Blood Coagulation Factors ◽  
Bovine Thrombin ◽  
Autoantibody Formation

Abstract Although antibody inhibitors directed against blood coagulation factors are well known, antibody inhibitors directed against thrombin are rare. We describe three postsurgical patients with prosthetic cardiac valves who developed serum autoantibodies reactive with human and bovine thrombin, as demonstrated by coagulation studies and immunoblotting. Despite marked prolongation of the thrombin time in these patients, the inhibitors were not associated with significant clinical bleeding. The mechanism of antithrombin autoantibody formation following surgery in patients with prosthetic cardiac valves remains to be determined.

scholarly journals Alterations of Blood Coagulation in Patients with Pancreatic Disorders

1977 ◽  
Author(s):  
H. Kästering ◽  
M. Hasenbein ◽  
C. Wendt ◽  
P.G. Lankisch
Keyword(s):  
Blood Coagulation ◽  
Coagulation Factors ◽  
Pancreatic Cysts ◽  
Thrombin Time ◽  
Consumption Coagulopathy ◽  
Blood Coagulation Factors ◽  
The Past ◽  
Α2 Macroglobulin ◽  
Pancreatic Disorders ◽  
Chronic Relapsing Pancreatitis

Hemorrhage as uell as thromboembolism is often found in patients uith pancreatic diseases. Ide systematically examined in the past tuo years blood coagulation factors in Ikpatients, uho uere admitted to our clinic uith elevated levels of amylases or uho were transfered from other hospitals after complications had occured.5B patients uith increased amylases (20 patients uith acute pancreatitis, 25 uith chronic relapsing pancreatitis, 2 uith concomitant pancreatitis, 11 uith pancreatic cysts) shoued no severe complications. In comparison to 26 normal persons ue found significant differences in the follouing results: enhancement of the thrombin generation in the TGT, shortening of the PTT and of the r- and k-time in the TEG, louered levels of α1-antitrypsin, α2-macroglobulin and heparin-like substances (Hiepler Test), increased levels in fibrinogen and fibrin monomere complexes, and decrease in platelets and in Quick’s Test. There uere no changes in the thrombin time, antithrombin III and plasminogen. In the group of 16 patients uith complications there uere only 3 cases of hypercoagulability, all others shoued signs of consumption-coagulopathy. The nine patients uho died all shoued signs Df acute renal failure and pancreatic lungs. Ue believe that the disturbances of blood coagulation in patients uith increased levels of amylases are initially caused by hypercoagulability and lead in some cases to thromboembolism, consumption-coagulopathy and DIC of the lungs and kidneys.

scholarly journals Changes in the blood coagulation system that determine postoperative complications in patients with non-tumor mechanical jaundice

2021 ◽  
Vol 180 (2) ◽  
pp. 12-20
Author(s):  
V. E. Fedorov ◽  
B. S. Kharitonov ◽  
A. D. Aslanov ◽  
O. E. Logvina ◽  
M. S. Narizhnaya
Keyword(s):  
Postoperative Complications ◽  
Blood Coagulation ◽  
Blood Platelets ◽  
Coagulation Factors ◽  
Coagulation System ◽  
Thrombin Time ◽  
Blood Coagulation Factors ◽  
Blood Coagulation System ◽  
Normal Limits ◽  
Prothrombin Index

The OBJECTIVE was to study the features of changes in the blood coagulation system that contribute to the development of postoperative complications in patients depending on the stage of non-tumor mechanical jaundice at admission.METHODS AND MATERIALS. A total of 537 patients with mechanical jaundice were examined and changes in the blood coagulation system were analyzed. Vascular-platelet hemostasis was characterized by the following tests: capillary resistance, the number of desquamated endothelial cells, the number of blood platelets. Plasma hemostasis was analyzed using activated partial thromboplastin time, plasma soluble fibrin level, thrombin time, prothrombin ratio, prothrombin index, and fibrinogen blood level. Then, XIIa-dependent fibrinolysis in the blood and the level of the fibrin D-dimer in the blood plasma were determined.RESULTS. It was found that in the first stage of mechanical jaundice, with cholestasis, there were no changes in blood coagulation system that go beyond the normal limits. In the second stage, during cytolysis of hepatocytes, hyperbilirubinemia and hypertransaminasemia contribute to the activation of platelet first, and then plasma hemostasis. In the third stage (cholangitis), the death of endotheliocytes increases and there is a deficiency of blood coagulation factors due to their consumption and increased fibrinolysis.CONCLUSION. In the stage of cholestasis in patients with non-tumors mechanical jaundice, the parameters of the coagulation system remain within the reference values. In the stage of cytolysis, as endotheliotoxicosis increases, platelet and plasma hemostasis begins to activate, which can lead to thrombosis and thromboembolism in vital organs. In the stage of cholangitis, further activation of plasma hemostasis causes hemorrhagic syndrome. The occurrence of the described disorders in blood coagulation system with the progression of MJ dictates the need to monitor the changes in the blood coagulation system and their correction for the prevention of intra-and postoperative complications.

Natural Oestrogen in the Female Climacteric - Influence on Blood Coagulation and Fibrinolysis

1978 ◽  
Vol 40 (02) ◽  
pp. 532-541 ◽  
Author(s):  
Anders Lagrelius ◽  
Nils-Olov Lunell ◽  
Margareta Blombäck
Keyword(s):  
Blood Coagulation ◽  
Antithrombin Iii ◽  
Coagulation Factors ◽  
Control Group ◽  
Blood Coagulation Factors ◽  
Excessive Bleeding ◽  
Oestrone Sulphate ◽  
Menopausal Women ◽  
History Of ◽  
Coagulation And Fibrinolysis

SummaryThe aim of the present study was to investigate the effect on blood coagulation and fibrinolysis of a natural oestrogen preparation, piperazine oestrone sulphate, prospectively in menopausal women. Scopolamine was given to the control group.The women were investigated before and during treatment with regard to factors VIII, VII, X, V, fibrinopeptide A, antithrombin III, plasminogen, rapid antiplasmin and α1-antitrypsin. There was no significant change towards hypercoagulability or decreased fibrinolysis in any group. In the oestrogen group, however, a tendency towards an increased level of plasminogen and a decreased level of antiplasmin was demonstrated. In the scopolamine group there was an unexpected fall in factors X and V and also in plasminogen and α1,-antitrypsin. A low level of some blood coagulation factors in some of the women before treatment is somewhat astonishing; none of them had any history of excessive bleeding.

Factor II Related Antigen and Antithrombin III Levels as Indicators of Liver Failure in Consumption Coagulopathy

1982 ◽  
Vol 47 (03) ◽  
pp. 218-220 ◽  
Author(s):  
P Sié ◽  
E Letrenne ◽  
C Caranobe ◽  
M Genestal ◽  
B Cathala ◽  
...  
Keyword(s):  
Liver Failure ◽  
Antithrombin Iii ◽  
Coagulation Factors ◽  
Consumption Coagulopathy ◽  
Blood Coagulation Factors ◽  
Factor Ii ◽  
At Iii ◽  
Group A ◽  
Group B

SummaryIn order to detect impaired synthesis of blood coagulation factors associated to consumption coagulopathy, a simultaneous evaluation of factor II-related antigen (II rAg) and of antithrombin III (AT III) was carried out in 16 patients affected with severe defibrination. An in vitro preliminary study on plasma and serum demonstrated that the levels of II rAg and of AT III, assessed by the Laurell technique with Behring antisera, were not reduced by the coagulation process. The patients were, a posteriori, classified into two groups according to the absence (group A) or the presence (group B) of factors predisposing to liver failure such as metastasis, cirrhosis, and prolonged shock. II rAg and AT III levels are significantly correlated; they are in the normal range in group A but reduced in group B. Thus II rAg or AT III level determinations are useful markers in the detection of liver failure associated to the consumption phenomenon. These results also suggest that part of the decreased AT III levels reported in severe cases of disseminated intravascular coagulation may be the consequence of an associated liver failure.

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