CD34 expression by stromal precursors in normal human adult bone marrow

Abstract Normal bone marrow cells were isolated by fluorescence-activated cell sorting (FACS) on the basis of CD34 antigen expression and then assayed in vitro for colonies of fibroblastic cells (fibroblast colony-forming units [CFU-F]). Greater than 95% of detectable CFU-F were recovered in the CD34+ population, while their numbers were markedly depleted in the CD34- population. Additional experiments showed that the majority of CFU-F exhibited high forward and perpendicular light scatter and low- density CD34 antigen. Growth of sorted cells in medium optimized for long-term marrow culture (LTMC) produced a complex mixture of adherent stromal elements including fibroblasts, adipocytes, smooth muscle cells, and macrophages. Monoclonal antibody STRO-1, which identifies bone marrow stromal cells, reacted with approximately 5% of CD34+ cells, which included all CFU-F and stromal precursors in LTMC. Experiments using soybean agglutinin (SBA) further showed that these stromal elements were restricted to a population of bone marrow cells with the phenotype CD34+/SBA+. These properties of stromal precursors are quite distinct from those of primitive hematopoietic progenitors, showing that although the precursors of the hematopoietic and stromal systems share expression of CD34, they are otherwise phenotypically distinct cell types.

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CD34 expression by stromal precursors in normal human adult bone marrow

Blood ◽

10.1182/blood.v78.11.2848.bloodjournal78112848 ◽

1991 ◽

Vol 78 (11) ◽

pp. 2848-2853 ◽

Cited By ~ 4

Author(s):

PJ Simmons ◽

B Torok-Storb

Keyword(s):

Bone Marrow ◽

Bone Marrow Cells ◽

Complex Mixture ◽

Antigen Expression ◽

Cell Types ◽

Stromal Precursors ◽

Cd34 Antigen ◽

Distinct Cell ◽

Marrow Cells

Normal bone marrow cells were isolated by fluorescence-activated cell sorting (FACS) on the basis of CD34 antigen expression and then assayed in vitro for colonies of fibroblastic cells (fibroblast colony-forming units [CFU-F]). Greater than 95% of detectable CFU-F were recovered in the CD34+ population, while their numbers were markedly depleted in the CD34- population. Additional experiments showed that the majority of CFU-F exhibited high forward and perpendicular light scatter and low- density CD34 antigen. Growth of sorted cells in medium optimized for long-term marrow culture (LTMC) produced a complex mixture of adherent stromal elements including fibroblasts, adipocytes, smooth muscle cells, and macrophages. Monoclonal antibody STRO-1, which identifies bone marrow stromal cells, reacted with approximately 5% of CD34+ cells, which included all CFU-F and stromal precursors in LTMC. Experiments using soybean agglutinin (SBA) further showed that these stromal elements were restricted to a population of bone marrow cells with the phenotype CD34+/SBA+. These properties of stromal precursors are quite distinct from those of primitive hematopoietic progenitors, showing that although the precursors of the hematopoietic and stromal systems share expression of CD34, they are otherwise phenotypically distinct cell types.

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THE ENHANCING EFFECT OF BONE MARROW CELLS ON THE PRIMARY IMMUNE RESPONSE OF THE ISOLATED PERFUSED SPLEEN

Journal of Experimental Medicine ◽

10.1084/jem.131.4.833 ◽

1970 ◽

Vol 131 (4) ◽

pp. 833-842 ◽

Cited By ~ 7

Author(s):

Robert C. Atkins ◽

William A. Robinson ◽

Ben Eiseman

Keyword(s):

Bone Marrow ◽

Antibody Response ◽

Bone Marrow Cells ◽

Cell Types ◽

Primary Immune Response ◽

Peripheral Lymphocytes ◽

Distinct Cell ◽

Primary Antibody Response ◽

Marrow Cells

The addition of bone marrow cells or peripheral lymphocytes to the isolated pig spleen markedly enhanced the primary antibody response after 3-day perfusion and antigenic challenge in vitro. The splenic preparation without added cells or with the addition of marrow cells to an irradiated spleen gave a limited response. Contributory evidence is provided that at least two distinct cell types are needed for antibody production. For optimal antibody response by an isolated perfused spleen, marrow cells or peripheral lymphocytes should be added to the system.

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Regulation of hematopoiesis: helper and suppressor influences of the thymus

Blood ◽

10.1182/blood.v55.3.524.524 ◽

1980 ◽

Vol 55 (3) ◽

pp. 524-527 ◽

Cited By ~ 26

Author(s):

SJ Sharkis ◽

JL Spivak ◽

A Ahmed ◽

J Misiti ◽

RK Stuart ◽

...

Keyword(s):

Bone Marrow ◽

Bone Marrow Cells ◽

Cell Types ◽

Regulatory Cells ◽

Plasma Clot ◽

Erythroid Progenitor ◽

Genetically Determined ◽

Regulatory Functions ◽

Marrow Cells

Abstract Thymocytes from normal mice strains as well as from genetically determined stem cell defective W/Wv anemic mice were cocultured with syngeneic (or congeneic) bone marrow cells. We assayed these cocultures for the proliferation of erythroid progenitor cell types (BFU-E and CFU- E) using the plasma clot technique. Results indicate that when concentrations of thymocytes were lower than bone marrow cells, significant suppression of erythroid growth was observed. However, when the concentration of thymocytes exceeded that of the bone marrow cells in culture (greater than 1:1), significant enhancement of erythroid growth was demonstrated. The W/Wv anemic bone marrow appears to respond to this interaction by enhancement at all concentrations of added normal thymocytes. The regulatory functions observed can be diminished by treatment of the thymocytes in vitro with anti-theta serum plus complement. Thus, we establish regulatory functions for anti-theta- sensitive regulatory cells (TSRC) with both positive (enhancement) and negative (suppression) components.

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Regulation of hematopoiesis: helper and suppressor influences of the thymus

Blood ◽

10.1182/blood.v55.3.524.bloodjournal553524 ◽

1980 ◽

Vol 55 (3) ◽

pp. 524-527

Author(s):

SJ Sharkis ◽

JL Spivak ◽

A Ahmed ◽

J Misiti ◽

RK Stuart ◽

...

Keyword(s):

Bone Marrow ◽

Bone Marrow Cells ◽

Cell Types ◽

Regulatory Cells ◽

Plasma Clot ◽

Erythroid Progenitor ◽

Genetically Determined ◽

Regulatory Functions ◽

Marrow Cells

Thymocytes from normal mice strains as well as from genetically determined stem cell defective W/Wv anemic mice were cocultured with syngeneic (or congeneic) bone marrow cells. We assayed these cocultures for the proliferation of erythroid progenitor cell types (BFU-E and CFU- E) using the plasma clot technique. Results indicate that when concentrations of thymocytes were lower than bone marrow cells, significant suppression of erythroid growth was observed. However, when the concentration of thymocytes exceeded that of the bone marrow cells in culture (greater than 1:1), significant enhancement of erythroid growth was demonstrated. The W/Wv anemic bone marrow appears to respond to this interaction by enhancement at all concentrations of added normal thymocytes. The regulatory functions observed can be diminished by treatment of the thymocytes in vitro with anti-theta serum plus complement. Thus, we establish regulatory functions for anti-theta- sensitive regulatory cells (TSRC) with both positive (enhancement) and negative (suppression) components.

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Parathyroid hormone activates adhesion in bone marrow stromal precursor cells

Journal of Endocrinology ◽

10.1677/joe.0.1800505 ◽

2004 ◽

Vol 180 (3) ◽

pp. 505-513 ◽

Cited By ~ 14

Author(s):

J Davies ◽

TJ Chambers

Keyword(s):

Bone Marrow ◽

Parathyroid Hormone ◽

Interleukin 6 ◽

Bone Marrow Cells ◽

Early Event ◽

Adherent Cells ◽

Anabolic Response ◽

Stromal Precursors ◽

Marrow Cells

The ability of parathyroid hormone (PTH) to enhance bone formation has recently been exploited in the treatment of osteoporosis. However, the underlying mechanisms are unknown. Osteoblasts, the bone-forming cells, derive from multipotential bone marrow stromal precursors called colony-forming units-fibroblastic (CFU-F) upon culture ex vivo. Adhesion of such stromal precursors to bone is likely to be an early event in the anabolic response of bone to PTH. To test this, we measured the number of CFU-F that could be extracted from murine bone marrow after administration of an anabolic dose of PTH. We found that a very early response is a dramatic reduction, starting within 2 h, in the number of CFU-F that could be extracted from their bone marrow. We then tested whether PTH has the ability to activate adhesion of CFU-F in vitro. For this, bone marrow cells were incubated in PTH for varying times. Non-adherent cells were then removed, and the adherent cells were incubated in PTH-free medium for 14 days to assess, as colony formation, the number of CFU-F that had adhered in the preceding period. We found that incubation in PTH caused a substantial increase in the number of CFU-F that adhered within 24 h. This increase was abrogated by peptidic inhibitors of integrins. The increase did not seem to be mediated through a PTH-induced increase in interleukin-6, since interleukin-6 had no effect on CFU-F numbers when substituted for PTH. Similarly, adhesion was unaffected by incubation of bone marrow cells in dibutyryl cyclic AMP, nor by inhibitors or donors of nitric oxide. However, activation of CFU-F in vitro by PTH was strongly inhibited by indomethacin and mimicked by prostaglandin E(2), and indomethacin reversed the PTH-mediated reduction of CFU-F that could be extracted from mouse bone marrow. These results suggested that PTH rapidly activates adhesion of CFU-F to plastic or bone surfaces. This activation may represent an early event in the anabolic response of bone cells to PTH.

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Effect of ligustrazine on CD34 antigen expression of bone marrow cells in immune-induced aplastic anemia mice

Chinese Journal of Integrative Medicine ◽

10.1007/bf02934173 ◽

1998 ◽

Vol 4 (2) ◽

pp. 148-148

Author(s):

Yanjun Shu ◽

Hanying Sun ◽

Lingli Dong ◽

Huizhen Xu ◽

Wu Lu ◽

...

Keyword(s):

Bone Marrow ◽

Aplastic Anemia ◽

Bone Marrow Cells ◽

Antigen Expression ◽

Cd34 Antigen ◽

Marrow Cells

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Influence of the drug of sodium diclofenac in a dose dl100 and dl50 on mechanisms of differentiation of cells of red bone marrow cells of rats in vitro

Problems of Uninterrupted Medical Training and Science ◽

10.31071/promedosvity2020.01.056 ◽

2020 ◽

Vol 2020 (1) ◽

pp. 56-61 ◽

Cited By ~ 1

Author(s):

A. S. Ivanov ◽

◽

S. O. Kondratov ◽

S. I. Skljar ◽

G. V. Zapko ◽

...

Keyword(s):

Bone Marrow ◽

Bone Marrow Cells ◽

Red Bone Marrow ◽

Sodium Diclofenac ◽

Marrow Cells

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Interleukin-3 is significantly more effective than other colony- stimulating factors in long-term maintenance of human bone marrow- derived colony-forming cells in vitro

Blood ◽

10.1182/blood.v73.7.1836.1836 ◽

1989 ◽

Vol 73 (7) ◽

pp. 1836-1841 ◽

Cited By ~ 13

Author(s):

M Kobayashi ◽

BH Van Leeuwen ◽

S Elsbury ◽

ME Martinson ◽

IG Young ◽

...

Keyword(s):

Bone Marrow ◽

Cultured Cells ◽

Bone Marrow Cells ◽

Human Bone ◽

Human Bone Marrow ◽

Interleukin 3 ◽

Gm Csf ◽

Factor G ◽

Marrow Cells

Abstract Human bone marrow cells cultured for 21 days in the presence of recombinant human interleukin-3 (IL-3) produced up to 28 times more colony-forming cells (CFC) than could be obtained from cultures stimulated with granulocyte colony stimulating factor (G-CSF) or granulocyte-macrophage CSF (GM-CSF). IL-3-cultured cells retained a multipotent response to IL-3 in colony assays but were restricted to formation of granulocyte colonies in G-CSF and granulocyte or macrophage colonies in GM-CSF. Culture of bone marrow cells in IL-3 also led to accumulation of large numbers of eosinophils and basophils. These data contrast with the effects of G-CSF, GM-CSF, and IL-3 in seven-day cultures. Here both GM-CSF and IL-3 amplified total CFC that had similar multipotential colony-forming capability in either factor. G-CSF, on the other hand, depleted IL-3-responsive colony-forming cells dramatically, apparently by causing these cells to mature into granulocytes. The data suggest that a large proportion of IL-3- responsive cells in human bone marrow express receptors for G-CSF and can respond to this factor, the majority becoming neutrophils. Furthermore, the CFC maintained for 21 days in IL-3 may be a functionally distinct population from that produced after seven days culture of bone marrow cells in either IL-3 or GM-CSF.

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Antioxidant Fusion Protein SOD1-Tat Increases the Engraftment Efficiency of Total Bone Marrow Cells in Irradiated Mice

Molecules ◽

10.3390/molecules26113395 ◽

2021 ◽

Vol 26 (11) ◽

pp. 3395

Author(s):

Ting Bei ◽

Xusong Cao ◽

Yun Liu ◽

Jinmei Li ◽

Haihua Luo ◽

...

Keyword(s):

Bone Marrow ◽

Fusion Protein ◽

Bone Marrow Cells ◽

Standard Procedure ◽

Severe Infection ◽

Copper Zinc Superoxide Dismutase ◽

Donor Cells ◽

Total Bone Marrow ◽

Marrow Cells

Total body irradiation is a standard procedure of bone marrow transplantation (BMT) which causes a rapid increase in reactive oxygen species (ROS) in the bone marrow microenvironment during BMT. The increase in ROS reduces the engraftment ability of donor cells, thereby affecting the bone marrow recovery of recipients after BMT. In the early weeks following transplantation, recipients are at high risk of severe infection due to weakened hematopoiesis. Thus, it is imperative to improve engraftment capacity and accelerate bone marrow recovery in BMT recipients. In this study, we constructed recombinant copper/zinc superoxide dismutase 1 (SOD1) fused with the cell-penetrating peptide (CPP), the trans-activator of transcription (Tat), and showed that this fusion protein has penetrating ability and antioxidant activity in both RAW264.7 cells and bone marrow cells in vitro. Furthermore, irradiated mice transplanted with SOD1-Tat-treated total bone marrow donor cells showed an increase in total bone marrow engraftment capacity two weeks after transplantation. This study explored an innovative method for enhancing engraftment efficiency and highlights the potential of CPP-SOD1 in ROS manipulation during BMT.

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