scholarly journals Optimization of heterologous protein production in Chinese hamster ovary cells under overexpression of spliced form of human X-box binding protein

2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Galina Gulis ◽  
Kelly Cristina Rodrigues Simi ◽  
Renata Rodrigues de Toledo ◽  
Andrea Queiroz Maranhao ◽  
Marcelo Macedo Brigido
Keyword(s):  
Chinese Hamster Ovary ◽  
Protein Production ◽  
Heterologous Protein ◽  
Binding Protein ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Heterologous Protein Production ◽  
Ovary Cells

Reduction of endogenous GRP78 levels improves secretion of a heterologous protein in CHO cells

1988 ◽  
Vol 8 (10) ◽  
pp. 4063-4070
Author(s):  
A J Dorner ◽  
M G Krane ◽  
R J Kaufman
Keyword(s):  
Endoplasmic Reticulum ◽  
Chinese Hamster Ovary ◽  
Heterologous Protein ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Glycosylation Site ◽  
Ovary Cells ◽  
Tissue Plasminogen ◽  
Stable Association

GRP78 is localized in the endoplasmic reticulum and associates with improperly folded or underglycosylated proteins. The role of GRP78 in secretion was studied in Chinese hamster ovary cells expressing a tissue plasminogen activator (tPA) variant which lacks potential N-linked glycosylation site sequences because of mutagenesis. The expression of variant tPA resulted in elevated levels of GRP78 and its stable association with tPA. The introduction of antisense GRP78 genes resulted in a two- to threefold reduction in GRP78 levels compared with those of the original cells. Cells with reduced levels of GRP78 secreted two- to threefold-higher levels of tPA activity. tPA expressed in these cells displayed reduced association with GRP78, and a greater proportion was processed to the mature form and secreted. These results demonstrate that reduction of GRP78 level can improve the secretion of an associated protein.

A strategy to accelerate protein production from a pool of clones in Chinese hamster ovary cells for toxicology studies

2017 ◽  
Vol 33 (6) ◽  
pp. 1449-1455 ◽  
Author(s):  
Zhilan Hu ◽  
Wendy Hsu ◽  
Abby Pynn ◽  
Domingos Ng ◽  
Donna Quicho ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Protein Production ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells
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